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Protein

Type-2 restriction enzyme KpnI

Gene

kpnIR

Organism
Klebsiella pneumoniae
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence GGTACC and cleaves after C-5.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi1180. KpnI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme KpnI (EC:3.1.21.4)
Short name:
R.KpnI
Alternative name(s):
Endonuclease KpnI
Type II restriction enzyme KpnI
Gene namesi
Name:kpnIR
OrganismiKlebsiella pneumoniae
Taxonomic identifieri573 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 218218Type-2 restriction enzyme KpnIPRO_0000077327Add
BLAST

Sequencei

Sequence statusi: Complete.

P25237-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVFDKVYSD DNNSYDQKTV SQRIEALFLN NLGKVVTRQQ IIRAATDPKT
60 70 80 90 100
GKQPENWHQR LSELRTDKGY TILSWRDMKV LAPQEYIMPH ATRRPKAAKR
110 120 130 140 150
VLPTKETWEQ VLDRANYSCE WQEDGQHCGL VEGDIDPIGG GTVKLTPDHM
160 170 180 190 200
TPHSIDPATD VNDPKMWQAL CGRHQVMKKN YWDSNNGKIN VIGILQSVNE
210
KQKNDALEFL LNYYGLKR
Length:218
Mass (Da):25,112
Last modified:May 1, 1992 - v1
Checksum:i857240D500E26C50
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M76435 Genomic DNA. Translation: AAA25089.1.
X61796 Genomic DNA. Translation: CAA43897.1.
PIRiS34431.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M76435 Genomic DNA. Translation: AAA25089.1.
X61796 Genomic DNA. Translation: CAA43897.1.
PIRiS34431.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi1180. KpnI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

ProtoNetiSearch...

Publicationsi

  1. "Genetic organization of the KpnI restriction-modification system."
    Chatterjee D.K., Hammond A.W., Blakesley R.W., Adams S.M., Gerard G.F.
    Nucleic Acids Res. 19:6505-6509(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-12.
    Strain: OK8.

Entry informationi

Entry nameiT2K1_KLEPN
AccessioniPrimary (citable) accession number: P25237
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: April 16, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.