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Protein

Type-2 restriction enzyme KpnI

Gene

kpnIR

Organism
Klebsiella pneumoniae
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence GGTACC and cleaves after C-5.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processRestriction system

Protein family/group databases

REBASEi1180 KpnI

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme KpnI (EC:3.1.21.4)
Short name:
R.KpnI
Alternative name(s):
Endonuclease KpnI
Type II restriction enzyme KpnI
Gene namesi
Name:kpnIR
OrganismiKlebsiella pneumoniae
Taxonomic identifieri573 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeKlebsiella

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000773271 – 218Type-2 restriction enzyme KpnIAdd BLAST218

Sequencei

Sequence statusi: Complete.

P25237-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVFDKVYSD DNNSYDQKTV SQRIEALFLN NLGKVVTRQQ IIRAATDPKT
60 70 80 90 100
GKQPENWHQR LSELRTDKGY TILSWRDMKV LAPQEYIMPH ATRRPKAAKR
110 120 130 140 150
VLPTKETWEQ VLDRANYSCE WQEDGQHCGL VEGDIDPIGG GTVKLTPDHM
160 170 180 190 200
TPHSIDPATD VNDPKMWQAL CGRHQVMKKN YWDSNNGKIN VIGILQSVNE
210
KQKNDALEFL LNYYGLKR
Length:218
Mass (Da):25,112
Last modified:May 1, 1992 - v1
Checksum:i857240D500E26C50
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M76435 Genomic DNA Translation: AAA25089.1
X61796 Genomic DNA Translation: CAA43897.1
PIRiS34431
RefSeqiWP_004176755.1, NZ_NRJD01000017.1

Genome annotation databases

PATRICifig|573.1567.peg.2312

Similar proteinsi

Entry informationi

Entry nameiT2K1_KLEPN
AccessioniPrimary (citable) accession number: P25237
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: May 23, 2018
This is version 55 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

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