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P25237 (T2K1_KLEPN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme KpnI

Short name=R.KpnI
EC=3.1.21.4
Alternative name(s):
Endonuclease KpnI
Type II restriction enzyme KpnI
Gene names
Name:kpnIR
OrganismKlebsiella pneumoniae
Taxonomic identifier573 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

Protein attributes

Sequence length218 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Recognizes the double-stranded sequence GGTACC and cleaves after C-5.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 218218Type-2 restriction enzyme KpnI
PRO_0000077327

Sequences

Sequence LengthMass (Da)Tools
P25237 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: 857240D500E26C50

FASTA21825,112
        10         20         30         40         50         60 
MDVFDKVYSD DNNSYDQKTV SQRIEALFLN NLGKVVTRQQ IIRAATDPKT GKQPENWHQR 

        70         80         90        100        110        120 
LSELRTDKGY TILSWRDMKV LAPQEYIMPH ATRRPKAAKR VLPTKETWEQ VLDRANYSCE 

       130        140        150        160        170        180 
WQEDGQHCGL VEGDIDPIGG GTVKLTPDHM TPHSIDPATD VNDPKMWQAL CGRHQVMKKN 

       190        200        210 
YWDSNNGKIN VIGILQSVNE KQKNDALEFL LNYYGLKR 

« Hide

References

[1]"Genetic organization of the KpnI restriction-modification system."
Chatterjee D.K., Hammond A.W., Blakesley R.W., Adams S.M., Gerard G.F.
Nucleic Acids Res. 19:6505-6509(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-12.
Strain: OK8.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M76435 Genomic DNA. Translation: AAA25089.1.
X61796 Genomic DNA. Translation: CAA43897.1.
PIRS34431.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE1180. KpnI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameT2K1_KLEPN
AccessionPrimary (citable) accession number: P25237
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: April 16, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries