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P25237

- T2K1_KLEPN

UniProt

P25237 - T2K1_KLEPN

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Protein

Type-2 restriction enzyme KpnI

Gene
kpnIR
Organism
Klebsiella pneumoniae
Status
Reviewed - Annotation score: 2 out of 5 - Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence GGTACC and cleaves after C-5.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi1180. KpnI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme KpnI (EC:3.1.21.4)
Short name:
R.KpnI
Alternative name(s):
Endonuclease KpnI
Type II restriction enzyme KpnI
Gene namesi
Name:kpnIR
OrganismiKlebsiella pneumoniae
Taxonomic identifieri573 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 218218Type-2 restriction enzyme KpnIPRO_0000077327Add
BLAST

Sequencei

Sequence statusi: Complete.

P25237-1 [UniParc]FASTAAdd to Basket

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MDVFDKVYSD DNNSYDQKTV SQRIEALFLN NLGKVVTRQQ IIRAATDPKT    50
GKQPENWHQR LSELRTDKGY TILSWRDMKV LAPQEYIMPH ATRRPKAAKR 100
VLPTKETWEQ VLDRANYSCE WQEDGQHCGL VEGDIDPIGG GTVKLTPDHM 150
TPHSIDPATD VNDPKMWQAL CGRHQVMKKN YWDSNNGKIN VIGILQSVNE 200
KQKNDALEFL LNYYGLKR 218
Length:218
Mass (Da):25,112
Last modified:May 1, 1992 - v1
Checksum:i857240D500E26C50
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M76435 Genomic DNA. Translation: AAA25089.1.
X61796 Genomic DNA. Translation: CAA43897.1.
PIRiS34431.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M76435 Genomic DNA. Translation: AAA25089.1 .
X61796 Genomic DNA. Translation: CAA43897.1 .
PIRi S34431.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 1180. KpnI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

ProtoNeti Search...

Publicationsi

  1. "Genetic organization of the KpnI restriction-modification system."
    Chatterjee D.K., Hammond A.W., Blakesley R.W., Adams S.M., Gerard G.F.
    Nucleic Acids Res. 19:6505-6509(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-12.
    Strain: OK8.

Entry informationi

Entry nameiT2K1_KLEPN
AccessioniPrimary (citable) accession number: P25237
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: April 16, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

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