P25237 (T2K1_KLEPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme KpnI
Short name=R.KpnI
EC=3.1.21.4

Alternative name(s):
Endonuclease KpnI
Type II restriction enzyme KpnI

Gene names

Name:

kpnIR

Organism

Klebsiella pneumoniae

Taxonomic identifier

573 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	218 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Recognizes the double-stranded sequence GGTACC and cleaves after C-5.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 218	218	Type-2 restriction enzyme KpnI		PRO_0000077327

Sequences

	Sequence		Length	Mass (Da)
	P25237 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 857240D500E26C50	FASTA	218	25,112
	10 20 30 40 50 60 MDVFDKVYSD DNNSYDQKTV SQRIEALFLN NLGKVVTRQQ IIRAATDPKT GKQPENWHQR 70 80 90 100 110 120 LSELRTDKGY TILSWRDMKV LAPQEYIMPH ATRRPKAAKR VLPTKETWEQ VLDRANYSCE 130 140 150 160 170 180 WQEDGQHCGL VEGDIDPIGG GTVKLTPDHM TPHSIDPATD VNDPKMWQAL CGRHQVMKKN 190 200 210 YWDSNNGKIN VIGILQSVNE KQKNDALEFL LNYYGLKR

References

[1]	"Genetic organization of the KpnI restriction-modification system." Chatterjee D.K., Hammond A.W., Blakesley R.W., Adams S.M., Gerard G.F. Nucleic Acids Res. 19:6505-6509(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-12. Strain: OK8.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M76435 Genomic DNA. Translation: AAA25089.1. X61796 Genomic DNA. Translation: CAA43897.1.
PIR	S34431.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	1180. KpnI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

T2K1_KLEPN

Accession

Primary (citable) accession number: P25237

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	April 3, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program