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P25237

- T2K1_KLEPN

UniProt

P25237 - T2K1_KLEPN

Protein

Type-2 restriction enzyme KpnI

Gene

kpnIR

Organism
Klebsiella pneumoniae
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 49 (16 Apr 2014)
      Sequence version 1 (01 May 1992)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence GGTACC and cleaves after C-5.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    GO - Molecular functioni

    1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Protein family/group databases

    REBASEi1180. KpnI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme KpnI (EC:3.1.21.4)
    Short name:
    R.KpnI
    Alternative name(s):
    Endonuclease KpnI
    Type II restriction enzyme KpnI
    Gene namesi
    Name:kpnIR
    OrganismiKlebsiella pneumoniae
    Taxonomic identifieri573 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 218218Type-2 restriction enzyme KpnIPRO_0000077327Add
    BLAST

    Sequencei

    Sequence statusi: Complete.

    P25237-1 [UniParc]FASTAAdd to Basket

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    MDVFDKVYSD DNNSYDQKTV SQRIEALFLN NLGKVVTRQQ IIRAATDPKT    50
    GKQPENWHQR LSELRTDKGY TILSWRDMKV LAPQEYIMPH ATRRPKAAKR 100
    VLPTKETWEQ VLDRANYSCE WQEDGQHCGL VEGDIDPIGG GTVKLTPDHM 150
    TPHSIDPATD VNDPKMWQAL CGRHQVMKKN YWDSNNGKIN VIGILQSVNE 200
    KQKNDALEFL LNYYGLKR 218
    Length:218
    Mass (Da):25,112
    Last modified:May 1, 1992 - v1
    Checksum:i857240D500E26C50
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M76435 Genomic DNA. Translation: AAA25089.1.
    X61796 Genomic DNA. Translation: CAA43897.1.
    PIRiS34431.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M76435 Genomic DNA. Translation: AAA25089.1 .
    X61796 Genomic DNA. Translation: CAA43897.1 .
    PIRi S34431.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 1180. KpnI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Genetic organization of the KpnI restriction-modification system."
      Chatterjee D.K., Hammond A.W., Blakesley R.W., Adams S.M., Gerard G.F.
      Nucleic Acids Res. 19:6505-6509(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-12.
      Strain: OK8.

    Entry informationi

    Entry nameiT2K1_KLEPN
    AccessioniPrimary (citable) accession number: P25237
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: May 1, 1992
    Last modified: April 16, 2014
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3