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P25235 (RPN2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2
EC=2.4.1.119
Alternative name(s):
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit
Ribophorin II
Short name=RPN-II
Ribophorin-2
Gene names
Name:Rpn2
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length631 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains By similarity.

Catalytic activity

Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.

Subunit structure

Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes By similarity.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein Potential.

Sequence similarities

Belongs to the SWP1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222
Chain23 – 631609Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2
PRO_0000022245

Regions

Topological domain23 – 540518Lumenal Potential
Transmembrane541 – 56121Helical; Potential
Transmembrane572 – 59221Helical; Potential
Transmembrane597 – 61721Helical; Potential
Topological domain618 – 63114Cytoplasmic Potential

Amino acid modifications

Modified residue2821Phosphothreonine By similarity
Glycosylation1061N-linked (GlcNAc...) Probable
Cross-link154Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Experimental info

Sequence conflict298 – 2992QP → HA in CAB56805. Ref.1
Sequence conflict3781V → A in CAB56805. Ref.1
Sequence conflict3901A → P in CAB56805. Ref.1
Sequence conflict4591Y → H in CAB56805. Ref.1
Sequence conflict575 – 5773STV → TTI in CAB56805. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P25235 [UniParc].

Last modified April 12, 2005. Version 2.
Checksum: 9BC0486C9C8469CD

FASTA63169,078
        10         20         30         40         50         60 
MAPPGSSAVF LLALTITAST QALTPTHYLT KHDVERLKAS LDRPFTSLES AFYSIVGLNS 

        70         80         90        100        110        120 
LGAQVPDVKK ACAFIKSNLD PSNVDSLFYA AQSSQVLSGC EISVSNETRD LLLAAVSEDS 

       130        140        150        160        170        180 
SVAQIYHAVA ALSGFGLPLA SHEALGALTA RLSKEETVLA TVQALHTASH LSQQADLRNI 

       190        200        210        220        230        240 
VEEIEDLVAR LDELGGVYLQ FEEGLELTAL FVAATYKLMD HVGTEPSIKE DQVIQLMNTI 

       250        260        270        280        290        300 
FSKKNFESLS EAFSVASAAA ALSQNRYHVP VVVVPEGSAS DTQEQAILRL QVSSVLSQPL 

       310        320        330        340        350        360 
AQAAVKLEHA KSVASRATVL QKMPFSLVGD VFELNFKNVK LPSGYYDFSV RVEGDNRYIA 

       370        380        390        400        410        420 
NTVELRVKIS TEVGITNVDL STVDKDQSIA PKTTRVTYPA KAKGTFIADS HQNFALFFQL 

       430        440        450        460        470        480 
VDVNTGAELT PHQTFVRLHN QKTGQEVVFV AEPDNKNVYK FELDTSERKI EFDSASGTYT 

       490        500        510        520        530        540 
LYLIIGDATL KNPILWNVAD VVIKFPEEEA PSTVLSQNLF TPKQEIQHLF REPEKRPPTV 

       550        560        570        580        590        600 
VSNTFTALIL SPLLLLFALW IRIGANVSNF TFAPSTVIFH LGHAAMLGLM YVYWTQLNMF 

       610        620        630 
QTLKYLAVLG TVTFLAGNRM LAQQAVKRTA H

« Hide

References

« Hide 'large scale' references
[1]"Rat ribophorin II: molecular cloning and chromosomal localization of a highly conserved transmembrane glycoprotein of the rough endoplasmic reticulum."
Pirozzi G., Zhou Z., D'Eustachio P., Sabatini D.D., Kreibich G.
Biochem. Biophys. Res. Commun. 176:1482-1486(1991) [PubMed: 1710116] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Pituitary.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X55298 mRNA. Translation: CAB56805.1.
BC060556 mRNA. Translation: AAH60556.1.
IPIIPI00188059.
PIRJN0065.
RefSeqNP_113886.1. NM_031698.1.
UniGeneRn.2879.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

IntActP25235. 1 interaction.
MINTMINT-4996562.
STRINGP25235.

Proteomic databases

PRIDEP25235.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000068135; ENSRNOP00000063207; ENSRNOG00000007492.
GeneID64701.
KEGGrno:64701.
UCSCNM_031698. rat.

Organism-specific databases

CTD6185.
RGD62075. Rpn2.

Phylogenomic databases

eggNOGroNOG05216.
GeneTreeENSGT00390000002635.
HOVERGENHBG002365.
InParanoidP25235.
PhylomeDBP25235.

Gene expression databases

ArrayExpressP25235.
GenevestigatorP25235.
GermOnlineENSRNOG00000007492. Rattus norvegicus.

Family and domain databases

InterProIPR008814. Ribophorin_II.
[Graphical view]
KOK12667.
PANTHERPTHR12640. Ribophorin_II. 1 hit.
PfamPF05817. Ribophorin_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio613702.

Entry information

Entry nameRPN2_RAT
AccessionPrimary (citable) accession number: P25235
Secondary accession number(s): Q6P9X0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: April 12, 2005
Last modified: November 16, 2011
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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