Reviewed, UniProtKB/Swiss-Prot P25235 (RPN2_RAT)

Names and origin

Protein names

Recommended name:
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2
    EC=2.4.1.119
Alternative name(s):
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit
    Ribophorin-2
    Ribophorin II
      Short name=RPN-II

Gene names

Name:

Rpn2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	631 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains By similarity.
Catalytic activity	Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.
Subunit structure	Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes By similarity.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the SWP1 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal Transmembrane
Molecular function	Transferase
PTM	Glycoprotein Isopeptide bond Phosphoprotein Ubl conjugation
Gene Ontology (GO)
Biological process	protein amino acid N-linked glycosylation via asparagine Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell oligosaccharyltransferase complex Inferred from electronic annotation. Source: InterPro rough microsome Inferred from direct assay. Source: RGD
Molecular function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22
	Chain	23 – 631	609	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2		PRO_0000022245
Regions
	Topological domain	23 – 540	518	Lumenal Potential
	Transmembrane	541 – 561	21	Potential
	Transmembrane	572 – 592	21	Potential
	Transmembrane	597 – 617	21	Potential
	Topological domain	618 – 631	14	Cytoplasmic Potential
Amino acid modifications
	Modified residue	282	1	Phosphothreonine By similarity
	Glycosylation	106	1	N-linked (GlcNAc...) Probable
	Cross-link	154		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Experimental info
	Sequence conflict	298 – 299	2	QP → HA in CAB56805. Ref.1
	Sequence conflict	378	1	V → A in CAB56805. Ref.1
	Sequence conflict	390	1	A → P in CAB56805. Ref.1
	Sequence conflict	459	1	Y → H in CAB56805. Ref.1
	Sequence conflict	575 – 577	3	STV → TTI in CAB56805. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P25235-1 [UniParc]. Last modified April 12, 2005. Version 2. Checksum: 9BC0486C9C8469CD	FASTA	631	69,078
	10 20 30 40 50 60 MAPPGSSAVF LLALTITAST QALTPTHYLT KHDVERLKAS LDRPFTSLES AFYSIVGLNS 70 80 90 100 110 120 LGAQVPDVKK ACAFIKSNLD PSNVDSLFYA AQSSQVLSGC EISVSNETRD LLLAAVSEDS 130 140 150 160 170 180 SVAQIYHAVA ALSGFGLPLA SHEALGALTA RLSKEETVLA TVQALHTASH LSQQADLRNI 190 200 210 220 230 240 VEEIEDLVAR LDELGGVYLQ FEEGLELTAL FVAATYKLMD HVGTEPSIKE DQVIQLMNTI 250 260 270 280 290 300 FSKKNFESLS EAFSVASAAA ALSQNRYHVP VVVVPEGSAS DTQEQAILRL QVSSVLSQPL 310 320 330 340 350 360 AQAAVKLEHA KSVASRATVL QKMPFSLVGD VFELNFKNVK LPSGYYDFSV RVEGDNRYIA 370 380 390 400 410 420 NTVELRVKIS TEVGITNVDL STVDKDQSIA PKTTRVTYPA KAKGTFIADS HQNFALFFQL 430 440 450 460 470 480 VDVNTGAELT PHQTFVRLHN QKTGQEVVFV AEPDNKNVYK FELDTSERKI EFDSASGTYT 490 500 510 520 530 540 LYLIIGDATL KNPILWNVAD VVIKFPEEEA PSTVLSQNLF TPKQEIQHLF REPEKRPPTV 550 560 570 580 590 600 VSNTFTALIL SPLLLLFALW IRIGANVSNF TFAPSTVIFH LGHAAMLGLM YVYWTQLNMF 610 620 630 QTLKYLAVLG TVTFLAGNRM LAQQAVKRTA H

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rat ribophorin II: molecular cloning and chromosomal localization of a highly conserved transmembrane glycoprotein of the rough endoplasmic reticulum." Pirozzi G., Zhou Z., D'Eustachio P., Sabatini D.D., Kreibich G. Biochem. Biophys. Res. Commun. 176:1482-1486(1991) [PubMed: 1710116] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pituitary.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X55298 mRNA. Translation: CAB56805.1. BC060556 mRNA. Translation: AAH60556.1.
IPI	IPI00188059.
PIR	JN0065.
RefSeq	NP_113886.1.
UniGene	Rn.2879
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P25235.
Proteomic databases
PRIDE	P25235.
Genome annotation databases
Ensembl	ENSRNOT00000068135; ENSRNOP00000063207; ENSRNOG00000007492; Rattus norvegicus. [Genome view]
GeneID	64701.
KEGG	rno:64701.
UCSC	NM_031698. rat.
Organism-specific databases
CTD	64701.
RGD	62075. Rpn2.
Phylogenomic databases
eggNOG	roNOG05216.
HOVERGEN	P25235.
InParanoid	P25235.
PhylomeDB	P25235.
Enzyme and pathway databases
BRENDA	2.4.1.119. 248.
Gene expression databases
ArrayExpress	P25235.
Genevestigator	P25235.
GermOnline	ENSRNOG00000007492. Rattus norvegicus.
Family and domain databases
InterPro	IPR008814. Ribophorin_II. [Graphical view]
PANTHER	PTHR12640. Ribophorin_II. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	613702.

Entry information

Entry name

RPN2_RAT

Accession

Primary (citable) accession number: P25235
Secondary accession number(s): Q6P9X0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	April 12, 2005
Last modified:	February 9, 2010
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)