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Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2

Gene

Rpn2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.By similarity

Catalytic activityi

Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • ribosome binding Source: RGD
  • transferase activity, transferring glycosyl groups Source: UniProtKB-KW

GO - Biological processi

  • aging Source: RGD
  • protein N-linked glycosylation Source: GO_Central
  • response to drug Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 (EC:2.4.99.18)
Alternative name(s):
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit
Ribophorin II
Short name:
RPN-II
Ribophorin-2
Gene namesi
Name:Rpn2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi62075. Rpn2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 540LumenalSequence analysisAdd BLAST518
Transmembranei541 – 561HelicalSequence analysisAdd BLAST21
Topological domaini562 – 571CytoplasmicSequence analysis10
Transmembranei572 – 592HelicalSequence analysisAdd BLAST21
Topological domaini593 – 596LumenalSequence analysis4
Transmembranei597 – 617HelicalSequence analysisAdd BLAST21
Topological domaini618 – 631CytoplasmicSequence analysisAdd BLAST14

GO - Cellular componenti

  • autophagosome membrane Source: RGD
  • integral component of membrane Source: UniProtKB-KW
  • nucleus Source: Ensembl
  • oligosaccharyltransferase complex Source: GO_Central
  • rough endoplasmic reticulum Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Add BLAST22
ChainiPRO_000002224523 – 631Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2Add BLAST609

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi106N-linked (GlcNAc...)Curated1
Cross-linki154Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Keywords - PTMi

Glycoprotein, Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiP25235.
PRIDEiP25235.

PTM databases

iPTMnetiP25235.
PhosphoSitePlusiP25235.
SwissPalmiP25235.

Expressioni

Gene expression databases

BgeeiENSRNOG00000007492.
ExpressionAtlasiP25235. differential.
GenevisibleiP25235. RN.

Interactioni

Subunit structurei

Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes. Also identified as part of a complex which includes CANX, DERL1, DERL2, DDOST/OST48, RPN1, RPN2, SELK, VIMP, STT3A AND VCP. This contains known members of the OST complex and may be a form of this complex (By similarity).By similarity

Protein-protein interaction databases

BioGridi249196. 2 interactors.
IntActiP25235. 2 interactors.
MINTiMINT-4996562.
STRINGi10116.ENSRNOP00000063207.

Family & Domainsi

Sequence similaritiesi

Belongs to the SWP1 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2447. Eukaryota.
ENOG410XRF2. LUCA.
GeneTreeiENSGT00390000002635.
HOGENOMiHOG000231261.
HOVERGENiHBG002365.
InParanoidiP25235.
KOiK12667.
OMAiVKKACAF.
OrthoDBiEOG091G0H3U.
PhylomeDBiP25235.

Family and domain databases

InterProiIPR008814. Swp1.
[Graphical view]
PANTHERiPTHR12640:SF0. PTHR12640:SF0. 1 hit.
PfamiPF05817. Ribophorin_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25235-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPPGSSAVF LLALTITAST QALTPTHYLT KHDVERLKAS LDRPFTSLES
60 70 80 90 100
AFYSIVGLNS LGAQVPDVKK ACAFIKSNLD PSNVDSLFYA AQSSQVLSGC
110 120 130 140 150
EISVSNETRD LLLAAVSEDS SVAQIYHAVA ALSGFGLPLA SHEALGALTA
160 170 180 190 200
RLSKEETVLA TVQALHTASH LSQQADLRNI VEEIEDLVAR LDELGGVYLQ
210 220 230 240 250
FEEGLELTAL FVAATYKLMD HVGTEPSIKE DQVIQLMNTI FSKKNFESLS
260 270 280 290 300
EAFSVASAAA ALSQNRYHVP VVVVPEGSAS DTQEQAILRL QVSSVLSQPL
310 320 330 340 350
AQAAVKLEHA KSVASRATVL QKMPFSLVGD VFELNFKNVK LPSGYYDFSV
360 370 380 390 400
RVEGDNRYIA NTVELRVKIS TEVGITNVDL STVDKDQSIA PKTTRVTYPA
410 420 430 440 450
KAKGTFIADS HQNFALFFQL VDVNTGAELT PHQTFVRLHN QKTGQEVVFV
460 470 480 490 500
AEPDNKNVYK FELDTSERKI EFDSASGTYT LYLIIGDATL KNPILWNVAD
510 520 530 540 550
VVIKFPEEEA PSTVLSQNLF TPKQEIQHLF REPEKRPPTV VSNTFTALIL
560 570 580 590 600
SPLLLLFALW IRIGANVSNF TFAPSTVIFH LGHAAMLGLM YVYWTQLNMF
610 620 630
QTLKYLAVLG TVTFLAGNRM LAQQAVKRTA H
Length:631
Mass (Da):69,078
Last modified:April 12, 2005 - v2
Checksum:i9BC0486C9C8469CD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti298 – 299QP → HA in CAB56805 (PubMed:1710116).Curated2
Sequence conflicti378V → A in CAB56805 (PubMed:1710116).Curated1
Sequence conflicti390A → P in CAB56805 (PubMed:1710116).Curated1
Sequence conflicti459Y → H in CAB56805 (PubMed:1710116).Curated1
Sequence conflicti575 – 577STV → TTI in CAB56805 (PubMed:1710116).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55298 mRNA. Translation: CAB56805.1.
BC060556 mRNA. Translation: AAH60556.1.
PIRiJN0065.
RefSeqiNP_113886.1. NM_031698.1.
UniGeneiRn.2879.

Genome annotation databases

EnsembliENSRNOT00000068135; ENSRNOP00000063207; ENSRNOG00000007492.
GeneIDi64701.
KEGGirno:64701.
UCSCiRGD:62075. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55298 mRNA. Translation: CAB56805.1.
BC060556 mRNA. Translation: AAH60556.1.
PIRiJN0065.
RefSeqiNP_113886.1. NM_031698.1.
UniGeneiRn.2879.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249196. 2 interactors.
IntActiP25235. 2 interactors.
MINTiMINT-4996562.
STRINGi10116.ENSRNOP00000063207.

PTM databases

iPTMnetiP25235.
PhosphoSitePlusiP25235.
SwissPalmiP25235.

Proteomic databases

PaxDbiP25235.
PRIDEiP25235.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000068135; ENSRNOP00000063207; ENSRNOG00000007492.
GeneIDi64701.
KEGGirno:64701.
UCSCiRGD:62075. rat.

Organism-specific databases

CTDi6185.
RGDi62075. Rpn2.

Phylogenomic databases

eggNOGiKOG2447. Eukaryota.
ENOG410XRF2. LUCA.
GeneTreeiENSGT00390000002635.
HOGENOMiHOG000231261.
HOVERGENiHBG002365.
InParanoidiP25235.
KOiK12667.
OMAiVKKACAF.
OrthoDBiEOG091G0H3U.
PhylomeDBiP25235.

Enzyme and pathway databases

UniPathwayiUPA00378.

Miscellaneous databases

PROiP25235.

Gene expression databases

BgeeiENSRNOG00000007492.
ExpressionAtlasiP25235. differential.
GenevisibleiP25235. RN.

Family and domain databases

InterProiIPR008814. Swp1.
[Graphical view]
PANTHERiPTHR12640:SF0. PTHR12640:SF0. 1 hit.
PfamiPF05817. Ribophorin_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRPN2_RAT
AccessioniPrimary (citable) accession number: P25235
Secondary accession number(s): Q6P9X0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: April 12, 2005
Last modified: November 30, 2016
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.