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Protein

Necdin

Gene

Ndn

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Growth suppressor that facilitates the entry of the cell into cell cycle arrest. Functionally similar to the retinoblastoma protein it binds to and represses the activity of cell-cycle-promoting proteins such as SV40 large T antigen, adenovirus E1A, and the transcription factor E2F. Necdin also interacts with p53 and works in an additive manner to inhibit cell growth. Functions also as transcription factor and binds directly to specific guanosine-rich DNA sequences.

GO - Molecular functioni

GO - Biological processi

  • axonal fasciculation Source: MGI
  • axon extension Source: MGI
  • axonogenesis Source: MGI
  • central nervous system development Source: MGI
  • genetic imprinting Source: MGI
  • glial cell migration Source: MGI
  • multicellular organismal homeostasis Source: MGI
  • negative regulation of cell proliferation Source: InterPro
  • neuron development Source: MGI
  • neuron migration Source: MGI
  • neurotrophin TRK receptor signaling pathway Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • post-embryonic development Source: MGI
  • regulation of growth Source: UniProtKB-KW
  • regulation of transcription, DNA-templated Source: MGI
  • respiratory gaseous exchange Source: MGI
  • respiratory system process Source: MGI
  • sensory perception of pain Source: MGI
  • transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Biological processi

Growth regulation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Necdin
Gene namesi
Name:Ndn
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:97290. Ndn.

Subcellular locationi

GO - Cellular componenti

  • cell projection Source: MGI
  • centrosome Source: MGI
  • cytoplasm Source: MGI
  • cytosol Source: MGI
  • nuclear matrix Source: UniProtKB-SubCell
  • nucleoplasm Source: UniProtKB-SubCell
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 325325NecdinPRO_0000156741Add
BLAST

Proteomic databases

MaxQBiP25233.
PaxDbiP25233.
PRIDEiP25233.

PTM databases

iPTMnetiP25233.
PhosphoSiteiP25233.

Expressioni

Tissue specificityi

Brain specific. Not detected in other tissues. Expressed in postmitotic neurons. In adult brain the highest expression is in hypothalamus. Highly expressed in thalamus and midbrain. Relatively low levels are in cerebral cortex, hippocampus, striatum, olfactory bulb, cerebellum, pons and spinal cord. Also detected in neurally differentiated embryonal carcinoma cells.

Developmental stagei

Expression levels were high during embryonic and neonatal periods (E14 to P7) and decreased thereafter.

Gene expression databases

BgeeiP25233.
CleanExiMM_NDN.
GenevisibleiP25233. MM.

Interactioni

Subunit structurei

Binds to the transactivation domains of E2F1 and p53. Binds also SV40 large T antigen and adenovirus E1A. Interacts with nucleobindin 1 and 2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
E2f1Q615015EBI-1801080,EBI-1025536
EID1Q9Y6B25EBI-1801080,EBI-1049975From a different organism.
Maged1Q9QYH67EBI-1801080,EBI-1801274
Msx1P132972EBI-1801080,EBI-903969
Msx2Q033583EBI-1801080,EBI-1801354
NGFRP081383EBI-1801080,EBI-1387782From a different organism.

GO - Molecular functioni

  • gamma-tubulin binding Source: MGI

Protein-protein interaction databases

BioGridi201712. 75 interactions.
IntActiP25233. 9 interactions.
MINTiMINT-1350583.
STRINGi10090.ENSMUSP00000045369.

Structurei

3D structure databases

ProteinModelPortaliP25233.
SMRiP25233. Positions 95-307.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini102 – 301200MAGEPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 MAGE domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4562. Eukaryota.
ENOG4111S70. LUCA.
GeneTreeiENSGT00760000118824.
HOGENOMiHOG000231161.
HOVERGENiHBG098101.
InParanoidiP25233.
KOiK19482.
OMAiRMIIWFP.
OrthoDBiEOG75F4GM.
PhylomeDBiP25233.
TreeFamiTF328505.

Family and domain databases

InterProiIPR002190. MHD_dom.
IPR030086. Necdin.
[Graphical view]
PANTHERiPTHR11736:SF10. PTHR11736:SF10. 1 hit.
PfamiPF01454. MAGE. 1 hit.
[Graphical view]
SMARTiSM01373. MAGE. 1 hit.
[Graphical view]
PROSITEiPS50838. MAGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25233-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEQSKDLSD PNFAAEVPDC EMQDSDAVPV GIPPPASLAA NLAGPPCAPE
60 70 80 90 100
GPMAAQQASP PPEERIEDVD PKILQQAAEE GRAHQPQSPA RPIPAPPAPA
110 120 130 140 150
QLVQKAHELM WYVLVKDQKR MVLWFPDMVK EVMGSYKKWC RSILRRTSVI
160 170 180 190 200
LARVFGLHLR LTNLHTMEFA LVKALSPEEL DRVALNNRMP MTGLLLMILS
210 220 230 240 250
LIYVKGRGAR EGAVWNVLRI LGLRPWKKHS TFGDVRKIIT EEFVQQNYLK
260 270 280 290 300
YQRVPHIEPP EYEFFWGSRA NREITKMQIM EFLARVFKKD PQAWPSRYRE
310 320
ALEQARALRE ANLAAQAPRS SVSED
Length:325
Mass (Da):36,832
Last modified:May 1, 1992 - v1
Checksum:i777385B0B75E443F
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti17 – 171V → A.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80840 mRNA. Translation: AAA39805.1.
D76440 Genomic DNA. Translation: BAA11183.1.
AK005078 mRNA. Translation: BAB23802.1.
AK075729 mRNA. Translation: BAC35914.1.
AK075735 mRNA. Translation: BAC35917.1.
AK076025 mRNA. Translation: BAC36130.1.
AK135444 mRNA. Translation: BAE22535.1.
AK149086 mRNA. Translation: BAE28734.1.
BC147249 mRNA. Translation: AAI47250.1.
BC147250 mRNA. Translation: AAI47251.1.
CCDSiCCDS21326.1.
PIRiJN0148.
RefSeqiNP_035012.2. NM_010882.3.
UniGeneiMm.400253.

Genome annotation databases

EnsembliENSMUST00000038775; ENSMUSP00000045369; ENSMUSG00000033585.
GeneIDi17984.
KEGGimmu:17984.
UCSCiuc009hff.1. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80840 mRNA. Translation: AAA39805.1.
D76440 Genomic DNA. Translation: BAA11183.1.
AK005078 mRNA. Translation: BAB23802.1.
AK075729 mRNA. Translation: BAC35914.1.
AK075735 mRNA. Translation: BAC35917.1.
AK076025 mRNA. Translation: BAC36130.1.
AK135444 mRNA. Translation: BAE22535.1.
AK149086 mRNA. Translation: BAE28734.1.
BC147249 mRNA. Translation: AAI47250.1.
BC147250 mRNA. Translation: AAI47251.1.
CCDSiCCDS21326.1.
PIRiJN0148.
RefSeqiNP_035012.2. NM_010882.3.
UniGeneiMm.400253.

3D structure databases

ProteinModelPortaliP25233.
SMRiP25233. Positions 95-307.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201712. 75 interactions.
IntActiP25233. 9 interactions.
MINTiMINT-1350583.
STRINGi10090.ENSMUSP00000045369.

PTM databases

iPTMnetiP25233.
PhosphoSiteiP25233.

Proteomic databases

MaxQBiP25233.
PaxDbiP25233.
PRIDEiP25233.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000038775; ENSMUSP00000045369; ENSMUSG00000033585.
GeneIDi17984.
KEGGimmu:17984.
UCSCiuc009hff.1. mouse.

Organism-specific databases

CTDi4692.
MGIiMGI:97290. Ndn.

Phylogenomic databases

eggNOGiKOG4562. Eukaryota.
ENOG4111S70. LUCA.
GeneTreeiENSGT00760000118824.
HOGENOMiHOG000231161.
HOVERGENiHBG098101.
InParanoidiP25233.
KOiK19482.
OMAiRMIIWFP.
OrthoDBiEOG75F4GM.
PhylomeDBiP25233.
TreeFamiTF328505.

Miscellaneous databases

ChiTaRSiNdn. mouse.
PROiP25233.
SOURCEiSearch...

Gene expression databases

BgeeiP25233.
CleanExiMM_NDN.
GenevisibleiP25233. MM.

Family and domain databases

InterProiIPR002190. MHD_dom.
IPR030086. Necdin.
[Graphical view]
PANTHERiPTHR11736:SF10. PTHR11736:SF10. 1 hit.
PfamiPF01454. MAGE. 1 hit.
[Graphical view]
SMARTiSM01373. MAGE. 1 hit.
[Graphical view]
PROSITEiPS50838. MAGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel brain-specific mRNA encoding nuclear protein (necdin) expressed in neurally differentiated embryonal carcinoma cells."
    Maruyama K., Usami M., Aizawa T., Yoshikawa K.
    Biochem. Biophys. Res. Commun. 178:291-296(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Embryonic carcinoma.
  2. "Structure and expression of the mouse necdin gene. Identification of a postmitotic neuron-restrictive core promoter."
    Uetsuki T., Takagi K., Sugiura H., Yoshikawa K.
    J. Biol. Chem. 271:918-924(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ALA-17.
    Strain: 129/Sv.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Cerebellum, Muellerian duct and Sympathetic ganglion.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Brain.
  5. "Necdin, a postmitotic neuron-specific growth suppressor, interacts with viral transforming proteins and cellular transcription factor E2F1."
    Taniura H., Taniguchi N., Hara M., Yoshikawa K.
    J. Biol. Chem. 273:720-728(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH VIRAL TRANSFORMING PROTEINS AND EF21.
  6. "Physical and functional interactions of neuronal growth suppressor necdin with p53."
    Taniura H., Matsumoto K., Yoshikawa K.
    J. Biol. Chem. 274:16242-16248(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TP53.
  7. "Cellular and subcellular localization of necdin in fetal and adult mouse brain."
    Niinobe M., Koyama K., Yoshikawa K.
    Dev. Neurosci. 22:310-319(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiNECD_MOUSE
AccessioniPrimary (citable) accession number: P25233
Secondary accession number(s): B9EJJ5, Q542W7, Q61951
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: June 8, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.