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Protein

Necdin

Gene

Ndn

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Growth suppressor that facilitates the entry of the cell into cell cycle arrest. Functionally similar to the retinoblastoma protein it binds to and represses the activity of cell-cycle-promoting proteins such as SV40 large T antigen, adenovirus E1A, and the transcription factor E2F. Necdin also interacts with p53 and works in an additive manner to inhibit cell growth. Functions also as transcription factor and binds directly to specific guanosine-rich DNA sequences.

GO - Molecular functioni

  • gamma-tubulin binding Source: MGI
  • RNA polymerase II proximal promoter sequence-specific DNA binding Source: MGI
  • transcriptional activator activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: MGI

GO - Biological processi

  • axonal fasciculation Source: MGI
  • axon extension Source: MGI
  • axonogenesis Source: MGI
  • central nervous system development Source: MGI
  • genetic imprinting Source: MGI
  • glial cell migration Source: MGI
  • multicellular organismal homeostasis Source: MGI
  • negative regulation of cell proliferation Source: InterPro
  • neuron development Source: MGI
  • neuron migration Source: MGI
  • neurotrophin TRK receptor signaling pathway Source: MGI
  • positive regulation of transcription by RNA polymerase II Source: MGI
  • post-embryonic development Source: MGI
  • regulation of growth Source: UniProtKB-KW
  • regulation of transcription, DNA-templated Source: MGI
  • respiratory gaseous exchange Source: MGI
  • respiratory system process Source: MGI
  • sensory perception of pain Source: MGI

Keywordsi

Molecular functionDNA-binding
Biological processGrowth regulation, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Necdin
Gene namesi
Name:Ndn
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:97290 Ndn

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001567411 – 325NecdinAdd BLAST325

Proteomic databases

MaxQBiP25233
PaxDbiP25233
PRIDEiP25233

PTM databases

iPTMnetiP25233
PhosphoSitePlusiP25233

Expressioni

Tissue specificityi

Brain specific. Not detected in other tissues. Expressed in postmitotic neurons. In adult brain the highest expression is in hypothalamus. Highly expressed in thalamus and midbrain. Relatively low levels are in cerebral cortex, hippocampus, striatum, olfactory bulb, cerebellum, pons and spinal cord. Also detected in neurally differentiated embryonal carcinoma cells.

Developmental stagei

Expression levels were high during embryonic and neonatal periods (E14 to P7) and decreased thereafter.

Gene expression databases

BgeeiENSMUSG00000033585
CleanExiMM_NDN
GenevisibleiP25233 MM

Interactioni

Subunit structurei

Binds to the transactivation domains of E2F1 and p53. Binds also SV40 large T antigen and adenovirus E1A. Interacts with nucleobindin 1 and 2.2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • gamma-tubulin binding Source: MGI

Protein-protein interaction databases

BioGridi201712, 79 interactors
IntActiP25233, 9 interactors
MINTiP25233
STRINGi10090.ENSMUSP00000045369

Structurei

3D structure databases

ProteinModelPortaliP25233
SMRiP25233
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini102 – 301MAGEPROSITE-ProRule annotationAdd BLAST200

Phylogenomic databases

eggNOGiKOG4562 Eukaryota
ENOG4111S70 LUCA
GeneTreeiENSGT00760000118824
HOGENOMiHOG000231161
HOVERGENiHBG098101
InParanoidiP25233
KOiK19482
OMAiRMIIWFP
OrthoDBiEOG091G0TJ0
PhylomeDBiP25233
TreeFamiTF328505

Family and domain databases

InterProiView protein in InterPro
IPR037445 MAGE
IPR002190 MHD_dom
IPR030086 Necdin
PANTHERiPTHR11736 PTHR11736, 1 hit
PTHR11736:SF10 PTHR11736:SF10, 1 hit
PfamiView protein in Pfam
PF01454 MAGE, 1 hit
SMARTiView protein in SMART
SM01373 MAGE, 1 hit
PROSITEiView protein in PROSITE
PS50838 MAGE, 1 hit

Sequencei

Sequence statusi: Complete.

P25233-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEQSKDLSD PNFAAEVPDC EMQDSDAVPV GIPPPASLAA NLAGPPCAPE
60 70 80 90 100
GPMAAQQASP PPEERIEDVD PKILQQAAEE GRAHQPQSPA RPIPAPPAPA
110 120 130 140 150
QLVQKAHELM WYVLVKDQKR MVLWFPDMVK EVMGSYKKWC RSILRRTSVI
160 170 180 190 200
LARVFGLHLR LTNLHTMEFA LVKALSPEEL DRVALNNRMP MTGLLLMILS
210 220 230 240 250
LIYVKGRGAR EGAVWNVLRI LGLRPWKKHS TFGDVRKIIT EEFVQQNYLK
260 270 280 290 300
YQRVPHIEPP EYEFFWGSRA NREITKMQIM EFLARVFKKD PQAWPSRYRE
310 320
ALEQARALRE ANLAAQAPRS SVSED
Length:325
Mass (Da):36,832
Last modified:May 1, 1992 - v1
Checksum:i777385B0B75E443F
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti17V → A1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80840 mRNA Translation: AAA39805.1
D76440 Genomic DNA Translation: BAA11183.1
AK005078 mRNA Translation: BAB23802.1
AK075729 mRNA Translation: BAC35914.1
AK075735 mRNA Translation: BAC35917.1
AK076025 mRNA Translation: BAC36130.1
AK135444 mRNA Translation: BAE22535.1
AK149086 mRNA Translation: BAE28734.1
BC147249 mRNA Translation: AAI47250.1
BC147250 mRNA Translation: AAI47251.1
CCDSiCCDS21326.1
PIRiJN0148
RefSeqiNP_035012.2, NM_010882.3
UniGeneiMm.400253

Genome annotation databases

EnsembliENSMUST00000038775; ENSMUSP00000045369; ENSMUSG00000033585
GeneIDi17984
KEGGimmu:17984
UCSCiuc009hff.1 mouse

Keywords - Coding sequence diversityi

Polymorphism

Entry informationi

Entry nameiNECD_MOUSE
AccessioniPrimary (citable) accession number: P25233
Secondary accession number(s): B9EJJ5, Q542W7, Q61951
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: March 28, 2018
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health