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Protein

Ras-related protein Rab-3

Gene

Rab3

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in exocytosis by regulating a late step in synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi28 – 36GTPBy similarity9
Nucleotide bindingi47 – 53GTPBy similarity7
Nucleotide bindingi76 – 80GTPBy similarity5
Nucleotide bindingi134 – 137GTPBy similarity4
Nucleotide bindingi164 – 166GTPBy similarity3

GO - Molecular functioni

GO - Biological processi

  • cytoskeletal matrix organization at active zone Source: FlyBase
  • exocytosis Source: UniProtKB-KW
  • maintenance of presynaptic active zone structure Source: FlyBase
  • neurotransmitter secretion Source: FlyBase
  • protein transport Source: UniProtKB-KW
  • Rab protein signal transduction Source: FlyBase
  • regulation of short-term neuronal synaptic plasticity Source: ParkinsonsUK-UCL
  • regulation of synaptic vesicle fusion to presynaptic membrane Source: ParkinsonsUK-UCL
  • vesicle-mediated transport Source: FlyBase
Complete GO annotation...

Keywords - Biological processi

Exocytosis, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-210500. Glutamate Neurotransmitter Release Cycle.
R-DME-264642. Acetylcholine Neurotransmitter Release Cycle.
R-DME-6798695. Neutrophil degranulation.
R-DME-8876198. RAB GEFs exchange GTP for GDP on RABs.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-3
Gene namesi
Name:Rab3
ORF Names:CG7576
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0005586. Rab3.

Subcellular locationi

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • presynaptic active zone Source: FlyBase
  • synapse Source: FlyBase
  • synaptic vesicle Source: FlyBase
  • vesicle Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001210921 – 220Ras-related protein Rab-3Add BLAST220

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi218S-geranylgeranyl cysteine1 Publication1
Modified residuei220Cysteine methyl esterBy similarity1
Lipidationi220S-geranylgeranyl cysteine1 Publication1

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

PaxDbiP25228.
PRIDEiP25228.

Expressioni

Gene expression databases

BgeeiFBgn0005586.
GenevisibleiP25228. DM.

Interactioni

Subunit structurei

Interacts with Rph.1 Publication

Protein-protein interaction databases

BioGridi61936. 6 interactors.
DIPiDIP-20159N.
MINTiMINT-946687.
STRINGi7227.FBpp0087352.

Structurei

Secondary structure

1220
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi19 – 29Combined sources11
Helixi34 – 43Combined sources10
Beta strandi55 – 65Combined sources11
Beta strandi68 – 77Combined sources10
Helixi81 – 83Combined sources3
Helixi84 – 89Combined sources6
Turni90 – 93Combined sources4
Beta strandi95 – 102Combined sources8
Helixi106 – 110Combined sources5
Helixi112 – 122Combined sources11
Beta strandi128 – 134Combined sources7
Helixi139 – 141Combined sources3
Helixi146 – 156Combined sources11
Beta strandi159 – 162Combined sources4
Turni165 – 168Combined sources4
Helixi171 – 185Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4RKFX-ray1.50A/B1-188[»]
ProteinModelPortaliP25228.
SMRiP25228.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi50 – 58Effector regionBy similarity9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0093. Eukaryota.
ENOG410ZZXQ. LUCA.
GeneTreeiENSGT00860000133677.
InParanoidiP25228.
KOiK07883.
OMAiQMASSQD.
OrthoDBiEOG091G0J64.
PhylomeDBiP25228.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25228-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASGGDPKWQ KDAADQNFDY MFKLLIIGNS SVGKTSFLFR YADDSFTSAF
60 70 80 90 100
VSTVGIDFKV KTVFRHDKRV KLQIWDTAGQ ERYRTITTAY YRGAMGFILM
110 120 130 140 150
YDVTNEDSFN SVQDWVTQIK TYSWDNAQVI LVGNKCDMED QRVISFERGR
160 170 180 190 200
QLADQLGVEF FETSAKENVN VKAVFERLVD IICDKMSESL DADPTLVGGG
210 220
QKGQRLTDQP QGTPNANCNC
Length:220
Mass (Da):24,862
Last modified:May 1, 1992 - v1
Checksum:iB324A77DBEF847A1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64621 mRNA. Translation: AAA28843.1.
AB112932 mRNA. Translation: BAD07037.1.
AE013599 Genomic DNA. Translation: AAF58762.1.
AY060449 mRNA. Translation: AAL25488.1.
PIRiJH0425.
RefSeqiNP_523687.1. NM_078963.3.
UniGeneiDm.2584.

Genome annotation databases

EnsemblMetazoaiFBtr0088257; FBpp0087352; FBgn0005586.
GeneIDi36127.
KEGGidme:Dmel_CG7576.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64621 mRNA. Translation: AAA28843.1.
AB112932 mRNA. Translation: BAD07037.1.
AE013599 Genomic DNA. Translation: AAF58762.1.
AY060449 mRNA. Translation: AAL25488.1.
PIRiJH0425.
RefSeqiNP_523687.1. NM_078963.3.
UniGeneiDm.2584.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4RKFX-ray1.50A/B1-188[»]
ProteinModelPortaliP25228.
SMRiP25228.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi61936. 6 interactors.
DIPiDIP-20159N.
MINTiMINT-946687.
STRINGi7227.FBpp0087352.

Proteomic databases

PaxDbiP25228.
PRIDEiP25228.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0088257; FBpp0087352; FBgn0005586.
GeneIDi36127.
KEGGidme:Dmel_CG7576.

Organism-specific databases

CTDi36127.
FlyBaseiFBgn0005586. Rab3.

Phylogenomic databases

eggNOGiKOG0093. Eukaryota.
ENOG410ZZXQ. LUCA.
GeneTreeiENSGT00860000133677.
InParanoidiP25228.
KOiK07883.
OMAiQMASSQD.
OrthoDBiEOG091G0J64.
PhylomeDBiP25228.

Enzyme and pathway databases

ReactomeiR-DME-210500. Glutamate Neurotransmitter Release Cycle.
R-DME-264642. Acetylcholine Neurotransmitter Release Cycle.
R-DME-6798695. Neutrophil degranulation.
R-DME-8876198. RAB GEFs exchange GTP for GDP on RABs.

Miscellaneous databases

ChiTaRSiRab3. fly.
GenomeRNAii36127.
PROiP25228.

Gene expression databases

BgeeiFBgn0005586.
GenevisibleiP25228. DM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRAB3_DROME
AccessioniPrimary (citable) accession number: P25228
Secondary accession number(s): Q9V5N9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: November 30, 2016
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.