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P25217

- T2BF_BACIU

UniProt

P25217 - T2BF_BACIU

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Protein

Type-2 restriction enzyme BsuFI

Gene

hsdFR

Organism
Bacillus subtilis
Status
Reviewed - Annotation score: 2 out of 5- Protein predictedi

Functioni

Recognizes the double-stranded sequence CCGG and cleaves after C-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactori

Magnesium.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Keywords - Ligandi

Magnesium

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme BsuFI (EC:3.1.21.4)
Short name:
R.BsuFI
Alternative name(s):
Endonuclease BsuFI
Type II restriction enzyme BsuFI
Gene namesi
Name:hsdFR
Synonyms:hsrF
OrganismiBacillus subtilis
Taxonomic identifieri1423 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 395395Type-2 restriction enzyme BsuFIPRO_0000077291Add
BLAST

Family & Domainsi

Family and domain databases

InterProiIPR011335. Restrct_endonuc-II-like.
IPR015291. Restrct_endonuc_II_MspI.
[Graphical view]
PfamiPF09208. Endonuc-MspI. 1 hit.
[Graphical view]
SUPFAMiSSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

P25217-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNKDNQIKNE SGKQAKILVS EIVNNLKNEL GINIEIEEGY SIGYPNQEKQ
60 70 80 90 100
FKMDFLVQFT DFDNEQWLIK STNSIRERIY GTEFFAQNIR LIDEKVKNIY
110 120 130 140 150
VVVPDSISSA EMKKKRNYSV KINGTTYTSF LTDVLTVNEL RQKIVEKASQ
160 170 180 190 200
NIAQGLRANV LGNDAETSIV NLLNDLKNKA LWNDYQNAQQ TIKSSTYKIY
210 220 230 240 250
KEILEKIDLK EGFDKILEVT ATNDIPLLSN RGKPKTDVSV TIKTNTKELI
260 270 280 290 300
RNISIKNTRE KTVTIHEGSV SDLISRLKLS ETDPLSQALI HFEKVGSKKK
310 320 330 340 350
LIAEHPNSDK ILEENLKLYN RELIEFLHSP LLNDKIQMVD LIIFTNKFAV
360 370 380 390
WNRDDYIKHY IEEYSGKGQF GTPFKWTYPS KKRGQKIQIK GFSNN
Length:395
Mass (Da):45,540
Last modified:May 1, 1992 - v1
Checksum:iC1E7D88FDAAC623D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X62104 Genomic DNA. Translation: CAA44014.1.
PIRiS20059.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X62104 Genomic DNA. Translation: CAA44014.1 .
PIRi S20059.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR011335. Restrct_endonuc-II-like.
IPR015291. Restrct_endonuc_II_MspI.
[Graphical view ]
Pfami PF09208. Endonuc-MspI. 1 hit.
[Graphical view ]
SUPFAMi SSF52980. SSF52980. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning, characterization and evolution of the BsuFI restriction endonuclease gene of Bacillus subtilis and purification of the enzyme."
    Kapfer W., Walter J., Trautner T.A.
    Nucleic Acids Res. 19:6457-6463(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ISF18.

Entry informationi

Entry nameiT2BF_BACIU
AccessioniPrimary (citable) accession number: P25217
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: April 16, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3