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P25217

- T2BF_BACIU

UniProt

P25217 - T2BF_BACIU

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Protein
Type-2 restriction enzyme BsuFI
Gene
hsdFR, hsrF
Organism
Bacillus subtilis
Status
Reviewed - Annotation score: 3 out of 5 - Protein predictedi

Functioni

Recognizes the double-stranded sequence CCGG and cleaves after C-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactori

Magnesium.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Keywords - Ligandi

Magnesium

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme BsuFI (EC:3.1.21.4)
Short name:
R.BsuFI
Alternative name(s):
Endonuclease BsuFI
Type II restriction enzyme BsuFI
Gene namesi
Name:hsdFR
Synonyms:hsrF
OrganismiBacillus subtilis
Taxonomic identifieri1423 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 395395Type-2 restriction enzyme BsuFI
PRO_0000077291Add
BLAST

Family & Domainsi

Family and domain databases

InterProiIPR011335. Restrct_endonuc-II-like.
IPR015291. Restrct_endonuc_II_MspI.
[Graphical view]
PfamiPF09208. Endonuc-MspI. 1 hit.
[Graphical view]
SUPFAMiSSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

P25217-1 [UniParc]FASTAAdd to Basket

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MNKDNQIKNE SGKQAKILVS EIVNNLKNEL GINIEIEEGY SIGYPNQEKQ    50
FKMDFLVQFT DFDNEQWLIK STNSIRERIY GTEFFAQNIR LIDEKVKNIY 100
VVVPDSISSA EMKKKRNYSV KINGTTYTSF LTDVLTVNEL RQKIVEKASQ 150
NIAQGLRANV LGNDAETSIV NLLNDLKNKA LWNDYQNAQQ TIKSSTYKIY 200
KEILEKIDLK EGFDKILEVT ATNDIPLLSN RGKPKTDVSV TIKTNTKELI 250
RNISIKNTRE KTVTIHEGSV SDLISRLKLS ETDPLSQALI HFEKVGSKKK 300
LIAEHPNSDK ILEENLKLYN RELIEFLHSP LLNDKIQMVD LIIFTNKFAV 350
WNRDDYIKHY IEEYSGKGQF GTPFKWTYPS KKRGQKIQIK GFSNN 395
Length:395
Mass (Da):45,540
Last modified:May 1, 1992 - v1
Checksum:iC1E7D88FDAAC623D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X62104 Genomic DNA. Translation: CAA44014.1.
PIRiS20059.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X62104 Genomic DNA. Translation: CAA44014.1 .
PIRi S20059.

3D structure databases

ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR011335. Restrct_endonuc-II-like.
IPR015291. Restrct_endonuc_II_MspI.
[Graphical view ]
Pfami PF09208. Endonuc-MspI. 1 hit.
[Graphical view ]
SUPFAMi SSF52980. SSF52980. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning, characterization and evolution of the BsuFI restriction endonuclease gene of Bacillus subtilis and purification of the enzyme."
    Kapfer W., Walter J., Trautner T.A.
    Nucleic Acids Res. 19:6457-6463(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ISF18.

Entry informationi

Entry nameiT2BF_BACIU
AccessioniPrimary (citable) accession number: P25217
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: April 16, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

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