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P25217 (T2BF_BACIU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme BsuFI

Short name=R.BsuFI
EC=3.1.21.4
Alternative name(s):
Endonuclease BsuFI
Type II restriction enzyme BsuFI
Gene names
Name:hsdFR
Synonyms:hsrF
OrganismBacillus subtilis
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length395 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence CCGG and cleaves after C-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactor

Magnesium.

Ontologies

Keywords
   Biological processRestriction system
   LigandMagnesium
   Molecular functionEndonuclease
Hydrolase
Nuclease
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

Type II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 395395Type-2 restriction enzyme BsuFI
PRO_0000077291

Sequences

Sequence LengthMass (Da)Tools
P25217 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: C1E7D88FDAAC623D

FASTA39545,540
        10         20         30         40         50         60 
MNKDNQIKNE SGKQAKILVS EIVNNLKNEL GINIEIEEGY SIGYPNQEKQ FKMDFLVQFT 

        70         80         90        100        110        120 
DFDNEQWLIK STNSIRERIY GTEFFAQNIR LIDEKVKNIY VVVPDSISSA EMKKKRNYSV 

       130        140        150        160        170        180 
KINGTTYTSF LTDVLTVNEL RQKIVEKASQ NIAQGLRANV LGNDAETSIV NLLNDLKNKA 

       190        200        210        220        230        240 
LWNDYQNAQQ TIKSSTYKIY KEILEKIDLK EGFDKILEVT ATNDIPLLSN RGKPKTDVSV 

       250        260        270        280        290        300 
TIKTNTKELI RNISIKNTRE KTVTIHEGSV SDLISRLKLS ETDPLSQALI HFEKVGSKKK 

       310        320        330        340        350        360 
LIAEHPNSDK ILEENLKLYN RELIEFLHSP LLNDKIQMVD LIIFTNKFAV WNRDDYIKHY 

       370        380        390 
IEEYSGKGQF GTPFKWTYPS KKRGQKIQIK GFSNN 

« Hide

References

[1]"Cloning, characterization and evolution of the BsuFI restriction endonuclease gene of Bacillus subtilis and purification of the enzyme."
Kapfer W., Walter J., Trautner T.A.
Nucleic Acids Res. 19:6457-6463(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ISF18.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X62104 Genomic DNA. Translation: CAA44014.1.
PIRS20059.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR011335. Restrct_endonuc-II-like.
IPR015291. Restrct_endonuc_II_MspI.
[Graphical view]
PfamPF09208. Endonuc-MspI. 1 hit.
[Graphical view]
SUPFAMSSF52980. SSF52980. 1 hit.
ProtoNetSearch...

Entry information

Entry nameT2BF_BACIU
AccessionPrimary (citable) accession number: P25217
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: April 16, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries