P25217 (T2BF_BACIU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme BsuFI
Short name=R.BsuFI
EC=3.1.21.4

Alternative name(s):
Endonuclease BsuFI
Type II restriction enzyme BsuFI

Gene names

Name:	hsdFR
Synonyms:	hsrF

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus subtilis group

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Recognizes the double-stranded sequence CCGG and cleaves after C-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.
Cofactor	Magnesium.

Ontologies

Keywords
Biological process	Restriction system
Ligand	Magnesium
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 395	395	Type-2 restriction enzyme BsuFI		PRO_0000077291

Sequences

	Sequence		Length	Mass (Da)
	P25217 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: C1E7D88FDAAC623D	FASTA	395	45,540
	10 20 30 40 50 60 MNKDNQIKNE SGKQAKILVS EIVNNLKNEL GINIEIEEGY SIGYPNQEKQ FKMDFLVQFT 70 80 90 100 110 120 DFDNEQWLIK STNSIRERIY GTEFFAQNIR LIDEKVKNIY VVVPDSISSA EMKKKRNYSV 130 140 150 160 170 180 KINGTTYTSF LTDVLTVNEL RQKIVEKASQ NIAQGLRANV LGNDAETSIV NLLNDLKNKA 190 200 210 220 230 240 LWNDYQNAQQ TIKSSTYKIY KEILEKIDLK EGFDKILEVT ATNDIPLLSN RGKPKTDVSV 250 260 270 280 290 300 TIKTNTKELI RNISIKNTRE KTVTIHEGSV SDLISRLKLS ETDPLSQALI HFEKVGSKKK 310 320 330 340 350 360 LIAEHPNSDK ILEENLKLYN RELIEFLHSP LLNDKIQMVD LIIFTNKFAV WNRDDYIKHY 370 380 390 IEEYSGKGQF GTPFKWTYPS KKRGQKIQIK GFSNN

References

[1]

"Cloning, characterization and evolution of the BsuFI restriction endonuclease gene of Bacillus subtilis and purification of the enzyme." Kapfer W., Walter J., Trautner T.A. Nucleic Acids Res. 19:6457-6463(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ISF18.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X62104 Genomic DNA. Translation: CAA44014.1.
PIR	S20059.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR011335. Restrct_endonuc-II-like. IPR015291. Restrct_endonuc_II_MspI. [Graphical view]
Pfam	PF09208. Endonuc-MspI. 1 hit. [Graphical view]
SUPFAM	SSF52980. Restrict_endonuc_II-like_core. 1 hit.
ProtoNet	Search...

Entry information

Entry name

T2BF_BACIU

Accession

Primary (citable) accession number: P25217

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	April 3, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program