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P25217

- T2BF_BACIU

UniProt

P25217 - T2BF_BACIU

Protein

Type-2 restriction enzyme BsuFI

Gene

hsdFR

Organism
Bacillus subtilis
Status
Reviewed - Annotation score: 2 out of 5- Protein predictedi
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    • History
      Entry version 61 (16 Apr 2014)
      Sequence version 1 (01 May 1992)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence CCGG and cleaves after C-1.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    Cofactori

    Magnesium.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    Magnesium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme BsuFI (EC:3.1.21.4)
    Short name:
    R.BsuFI
    Alternative name(s):
    Endonuclease BsuFI
    Type II restriction enzyme BsuFI
    Gene namesi
    Name:hsdFR
    Synonyms:hsrF
    OrganismiBacillus subtilis
    Taxonomic identifieri1423 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 395395Type-2 restriction enzyme BsuFIPRO_0000077291Add
    BLAST

    Family & Domainsi

    Family and domain databases

    InterProiIPR011335. Restrct_endonuc-II-like.
    IPR015291. Restrct_endonuc_II_MspI.
    [Graphical view]
    PfamiPF09208. Endonuc-MspI. 1 hit.
    [Graphical view]
    SUPFAMiSSF52980. SSF52980. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P25217-1 [UniParc]FASTAAdd to Basket

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    MNKDNQIKNE SGKQAKILVS EIVNNLKNEL GINIEIEEGY SIGYPNQEKQ    50
    FKMDFLVQFT DFDNEQWLIK STNSIRERIY GTEFFAQNIR LIDEKVKNIY 100
    VVVPDSISSA EMKKKRNYSV KINGTTYTSF LTDVLTVNEL RQKIVEKASQ 150
    NIAQGLRANV LGNDAETSIV NLLNDLKNKA LWNDYQNAQQ TIKSSTYKIY 200
    KEILEKIDLK EGFDKILEVT ATNDIPLLSN RGKPKTDVSV TIKTNTKELI 250
    RNISIKNTRE KTVTIHEGSV SDLISRLKLS ETDPLSQALI HFEKVGSKKK 300
    LIAEHPNSDK ILEENLKLYN RELIEFLHSP LLNDKIQMVD LIIFTNKFAV 350
    WNRDDYIKHY IEEYSGKGQF GTPFKWTYPS KKRGQKIQIK GFSNN 395
    Length:395
    Mass (Da):45,540
    Last modified:May 1, 1992 - v1
    Checksum:iC1E7D88FDAAC623D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X62104 Genomic DNA. Translation: CAA44014.1.
    PIRiS20059.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X62104 Genomic DNA. Translation: CAA44014.1 .
    PIRi S20059.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR011335. Restrct_endonuc-II-like.
    IPR015291. Restrct_endonuc_II_MspI.
    [Graphical view ]
    Pfami PF09208. Endonuc-MspI. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52980. SSF52980. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, characterization and evolution of the BsuFI restriction endonuclease gene of Bacillus subtilis and purification of the enzyme."
      Kapfer W., Walter J., Trautner T.A.
      Nucleic Acids Res. 19:6457-6463(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ISF18.

    Entry informationi

    Entry nameiT2BF_BACIU
    AccessioniPrimary (citable) accession number: P25217
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: May 1, 1992
    Last modified: April 16, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3