Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA replication licensing factor MCM3

Gene

Mcm3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi345 – 352ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Cell cycle, DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-176187. Activation of ATR in response to replication stress.
R-MMU-68867. Assembly of the pre-replicative complex.
R-MMU-68949. Orc1 removal from chromatin.
R-MMU-68962. Activation of the pre-replicative complex.
R-MMU-69052. Switching of origins to a post-replicative state.
R-MMU-69300. Removal of licensing factors from origins.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor MCM3 (EC:3.6.4.12)
Alternative name(s):
DNA polymerase alpha holoenzyme-associated protein P1
P1-MCM3
Gene namesi
Name:Mcm3
Synonyms:Mcmd, Mcmd3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:101845. Mcm3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001940942 – 812DNA replication licensing factor MCM3Add BLAST811

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei160PhosphoserineCombined sources1
Modified residuei293N6-acetyllysineCombined sources1
Modified residuei547N6-acetyllysineCombined sources1
Modified residuei611PhosphoserineBy similarity1
Modified residuei668PhosphoserineCombined sources1
Modified residuei672PhosphoserineCombined sources1
Modified residuei681PhosphoserineBy similarity1
Modified residuei705PhosphotyrosineBy similarity1
Modified residuei708PhosphoserineCombined sources1
Modified residuei719PhosphothreonineCombined sources1
Modified residuei722PhosphothreonineBy similarity1
Modified residuei729PhosphothreonineCombined sources1
Modified residuei732PhosphoserineCombined sources1
Modified residuei738PhosphoserineCombined sources1

Post-translational modificationi

O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.By similarity

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP25206.
PaxDbiP25206.
PeptideAtlasiP25206.
PRIDEiP25206.

PTM databases

iPTMnetiP25206.
PhosphoSitePlusiP25206.
SwissPalmiP25206.

Miscellaneous databases

PMAP-CutDBP25206.

Expressioni

Gene expression databases

BgeeiENSMUSG00000041859.
ExpressionAtlasiP25206. baseline and differential.
GenevisibleiP25206. MM.

Interactioni

Subunit structurei

Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (Probable). Associated with the replication-specific DNA polymerase alpha. Interacts with MCMBP. Interacts with ANKRD17.By similarityCurated

Protein-protein interaction databases

BioGridi201345. 29 interactors.
DIPiDIP-45878N.
IntActiP25206. 26 interactors.
MINTiMINT-4101604.
STRINGi10090.ENSMUSP00000059192.

Structurei

3D structure databases

ProteinModelPortaliP25206.
SMRiP25206.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini295 – 502MCMAdd BLAST208

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi477 – 480Arginine finger4

Sequence similaritiesi

Belongs to the MCM family.Curated
Contains 1 MCM domain.Curated

Phylogenomic databases

eggNOGiKOG0479. Eukaryota.
COG1241. LUCA.
GeneTreeiENSGT00550000075022.
HOGENOMiHOG000224126.
HOVERGENiHBG104962.
InParanoidiP25206.
KOiK02541.
OMAiLGSMVCV.
OrthoDBiEOG091G02B0.
PhylomeDBiP25206.
TreeFamiTF106459.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR031327. MCM.
IPR008046. Mcm3.
IPR018525. MCM_CS.
IPR001208. MCM_dom.
IPR027925. MCM_N.
IPR033762. MCM_OB.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
PF17207. MCM_OB. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
PR01659. MCMPROTEIN3.
SMARTiSM00382. AAA. 1 hit.
SM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25206-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGTVVLDDV ELREAQRDYL DFLDDEEDQG IYQNKVRELI SDNQYRLIVS
60 70 80 90 100
VNDLRRKNEK RANRLLNNAF EELVAFQRAL KDFVASIDAT YAKQYEEFYI
110 120 130 140 150
GLEGSFGSKH VSPRTLTSCF LSCVVCVEGI VTKCSLVRPK VVRSVHYCPA
160 170 180 190 200
TKKTIERRYS DLTTLVAFPS SSVYPTKDEE NNPLETEYGL SVYKDHQTIT
210 220 230 240 250
IQEMPEKAPA GQLPRSVDVI LDDDLVDKVK PGDRIQVVGT YRCLPGKKGC
260 270 280 290 300
YTSGTFRTVL IACNVKQMSK DIQPAFSADD IAKIKKFSKT RSKDVFEQLA
310 320 330 340 350
RSLAPSIHGH DYVKKAILCL LLGGVERELE NGSHIRGDIN ILLIGDPSVA
360 370 380 390 400
KSQLLRYVLC TAPRAIPTTG RGSSGVGLTA AVTTDQETGE RRLEAGAMVL
410 420 430 440 450
ADRGVVCIDE FDKMSDMDRT AIHEVMEQGR VTIAKAGIHA RLNARCSVLA
460 470 480 490 500
AANPVYGRYD QYKTPMENIG LQDSLLSRFD LLFIMLDQMD PEQDREISDH
510 520 530 540 550
VLRMHQYRAP GEQDGDALPL GSSVDILATD DPDFTQDDQQ DTRIYEKHDS
560 570 580 590 600
LLHGTKKKKE KMVSAAFMKK YIHVAKIIKP TLTQESAAYI AEEYSRLRSQ
610 620 630 640 650
DSMSSDTART SPVTARTLET LIRLATAHAK ARMSKTVDLQ DAEEAVELVQ
660 670 680 690 700
YAYFKKVLEK EKKRKKASED ESDLEDEEEK SQEDTEQKRK RRKTHAKDGE
710 720 730 740 750
SYDPYDFSEA ETQMPQVHTP KTDDSQEKTD DSQETQDSQK VELSEPRLKA
760 770 780 790 800
FKAALLEVFQ EAHEQSVGML HLTESINRNR EEPFSSEEIQ ACLSRMQDDN
810
QVMVSEGIVF LI
Length:812
Mass (Da):91,546
Last modified:November 1, 1997 - v2
Checksum:i3C8653CF13D0F140
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti18 – 22DYLDF → RLLGL in CAA44079 (PubMed:1549468).Curated5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC031700 mRNA. Translation: AAH31700.1.
X62154 mRNA. Translation: CAA44079.1.
D26088 Genomic DNA. Translation: BAA05081.1.
CCDSiCCDS14843.1.
PIRiS22804.
S51615.
RefSeqiNP_032589.1. NM_008563.2.
UniGeneiMm.4502.

Genome annotation databases

EnsembliENSMUST00000053266; ENSMUSP00000059192; ENSMUSG00000041859.
GeneIDi17215.
KEGGimmu:17215.
UCSCiuc007alc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC031700 mRNA. Translation: AAH31700.1.
X62154 mRNA. Translation: CAA44079.1.
D26088 Genomic DNA. Translation: BAA05081.1.
CCDSiCCDS14843.1.
PIRiS22804.
S51615.
RefSeqiNP_032589.1. NM_008563.2.
UniGeneiMm.4502.

3D structure databases

ProteinModelPortaliP25206.
SMRiP25206.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201345. 29 interactors.
DIPiDIP-45878N.
IntActiP25206. 26 interactors.
MINTiMINT-4101604.
STRINGi10090.ENSMUSP00000059192.

PTM databases

iPTMnetiP25206.
PhosphoSitePlusiP25206.
SwissPalmiP25206.

Proteomic databases

EPDiP25206.
PaxDbiP25206.
PeptideAtlasiP25206.
PRIDEiP25206.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000053266; ENSMUSP00000059192; ENSMUSG00000041859.
GeneIDi17215.
KEGGimmu:17215.
UCSCiuc007alc.1. mouse.

Organism-specific databases

CTDi4172.
MGIiMGI:101845. Mcm3.

Phylogenomic databases

eggNOGiKOG0479. Eukaryota.
COG1241. LUCA.
GeneTreeiENSGT00550000075022.
HOGENOMiHOG000224126.
HOVERGENiHBG104962.
InParanoidiP25206.
KOiK02541.
OMAiLGSMVCV.
OrthoDBiEOG091G02B0.
PhylomeDBiP25206.
TreeFamiTF106459.

Enzyme and pathway databases

ReactomeiR-MMU-176187. Activation of ATR in response to replication stress.
R-MMU-68867. Assembly of the pre-replicative complex.
R-MMU-68949. Orc1 removal from chromatin.
R-MMU-68962. Activation of the pre-replicative complex.
R-MMU-69052. Switching of origins to a post-replicative state.
R-MMU-69300. Removal of licensing factors from origins.

Miscellaneous databases

ChiTaRSiMcm3. mouse.
PMAP-CutDBP25206.
PROiP25206.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000041859.
ExpressionAtlasiP25206. baseline and differential.
GenevisibleiP25206. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR031327. MCM.
IPR008046. Mcm3.
IPR018525. MCM_CS.
IPR001208. MCM_dom.
IPR027925. MCM_N.
IPR033762. MCM_OB.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
PF17207. MCM_OB. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
PR01659. MCMPROTEIN3.
SMARTiSM00382. AAA. 1 hit.
SM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMCM3_MOUSE
AccessioniPrimary (citable) accession number: P25206
Secondary accession number(s): Q61492
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: November 1, 1997
Last modified: November 30, 2016
This is version 158 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.