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P25206

- MCM3_MOUSE

UniProt

P25206 - MCM3_MOUSE

Protein

DNA replication licensing factor MCM3

Gene

Mcm3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 135 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi345 – 3528ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. DNA binding Source: UniProtKB-KW
    3. DNA helicase activity Source: InterPro

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. DNA replication initiation Source: InterPro

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Biological processi

    Cell cycle, DNA replication

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_217374. Unwinding of DNA.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA replication licensing factor MCM3 (EC:3.6.4.12)
    Alternative name(s):
    DNA polymerase alpha holoenzyme-associated protein P1
    P1-MCM3
    Gene namesi
    Name:Mcm3
    Synonyms:Mcmd, Mcmd3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:101845. Mcm3.

    Subcellular locationi

    GO - Cellular componenti

    1. centrosome Source: Ensembl
    2. cytoplasm Source: MGI
    3. MCM complex Source: UniProtKB
    4. nucleus Source: MGI
    5. perinuclear region of cytoplasm Source: Ensembl

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 812811DNA replication licensing factor MCM3PRO_0000194094Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei160 – 1601PhosphoserineBy similarity
    Modified residuei293 – 2931N6-acetyllysine1 Publication
    Modified residuei547 – 5471N6-acetyllysine1 Publication
    Modified residuei668 – 6681Phosphoserine1 Publication
    Modified residuei672 – 6721Phosphoserine2 Publications
    Modified residuei681 – 6811PhosphoserineBy similarity
    Modified residuei705 – 7051PhosphotyrosineBy similarity
    Modified residuei708 – 7081PhosphoserineBy similarity
    Modified residuei719 – 7191PhosphothreonineBy similarity
    Modified residuei722 – 7221PhosphothreonineBy similarity

    Post-translational modificationi

    O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.By similarity

    Keywords - PTMi

    Acetylation, Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiP25206.
    PaxDbiP25206.
    PRIDEiP25206.

    PTM databases

    PhosphoSiteiP25206.

    Miscellaneous databases

    PMAP-CutDBP25206.

    Expressioni

    Gene expression databases

    ArrayExpressiP25206.
    BgeeiP25206.
    GenevestigatoriP25206.

    Interactioni

    Subunit structurei

    Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5. Associated with the replication-specific DNA polymerase alpha. Interacts with MCMBP (By simililarity).

    Protein-protein interaction databases

    BioGridi201345. 4 interactions.
    DIPiDIP-45878N.
    IntActiP25206. 1 interaction.
    MINTiMINT-4101604.

    Structurei

    3D structure databases

    ProteinModelPortaliP25206.
    SMRiP25206. Positions 10-650.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini295 – 502208MCMAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi477 – 4804Arginine finger

    Sequence similaritiesi

    Belongs to the MCM family.Curated
    Contains 1 MCM domain.Curated

    Phylogenomic databases

    eggNOGiCOG1241.
    HOGENOMiHOG000224126.
    HOVERGENiHBG104962.
    InParanoidiP25206.
    KOiK02541.
    OMAiRMISEHV.
    OrthoDBiEOG7V1FQ3.
    PhylomeDBiP25206.
    TreeFamiTF106459.

    Family and domain databases

    Gene3Di2.40.50.140. 2 hits.
    3.40.50.300. 1 hit.
    InterProiIPR003593. AAA+_ATPase.
    IPR008046. Mcm3.
    IPR018525. MCM_CS.
    IPR001208. MCM_DNA-dep_ATPase.
    IPR027925. MCM_N.
    IPR012340. NA-bd_OB-fold.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00493. MCM. 1 hit.
    PF14551. MCM_N. 1 hit.
    [Graphical view]
    PRINTSiPR01657. MCMFAMILY.
    PR01659. MCMPROTEIN3.
    SMARTiSM00382. AAA. 1 hit.
    SM00350. MCM. 1 hit.
    [Graphical view]
    SUPFAMiSSF50249. SSF50249. 1 hit.
    SSF52540. SSF52540. 2 hits.
    PROSITEiPS00847. MCM_1. 1 hit.
    PS50051. MCM_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P25206-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGTVVLDDV ELREAQRDYL DFLDDEEDQG IYQNKVRELI SDNQYRLIVS    50
    VNDLRRKNEK RANRLLNNAF EELVAFQRAL KDFVASIDAT YAKQYEEFYI 100
    GLEGSFGSKH VSPRTLTSCF LSCVVCVEGI VTKCSLVRPK VVRSVHYCPA 150
    TKKTIERRYS DLTTLVAFPS SSVYPTKDEE NNPLETEYGL SVYKDHQTIT 200
    IQEMPEKAPA GQLPRSVDVI LDDDLVDKVK PGDRIQVVGT YRCLPGKKGC 250
    YTSGTFRTVL IACNVKQMSK DIQPAFSADD IAKIKKFSKT RSKDVFEQLA 300
    RSLAPSIHGH DYVKKAILCL LLGGVERELE NGSHIRGDIN ILLIGDPSVA 350
    KSQLLRYVLC TAPRAIPTTG RGSSGVGLTA AVTTDQETGE RRLEAGAMVL 400
    ADRGVVCIDE FDKMSDMDRT AIHEVMEQGR VTIAKAGIHA RLNARCSVLA 450
    AANPVYGRYD QYKTPMENIG LQDSLLSRFD LLFIMLDQMD PEQDREISDH 500
    VLRMHQYRAP GEQDGDALPL GSSVDILATD DPDFTQDDQQ DTRIYEKHDS 550
    LLHGTKKKKE KMVSAAFMKK YIHVAKIIKP TLTQESAAYI AEEYSRLRSQ 600
    DSMSSDTART SPVTARTLET LIRLATAHAK ARMSKTVDLQ DAEEAVELVQ 650
    YAYFKKVLEK EKKRKKASED ESDLEDEEEK SQEDTEQKRK RRKTHAKDGE 700
    SYDPYDFSEA ETQMPQVHTP KTDDSQEKTD DSQETQDSQK VELSEPRLKA 750
    FKAALLEVFQ EAHEQSVGML HLTESINRNR EEPFSSEEIQ ACLSRMQDDN 800
    QVMVSEGIVF LI 812
    Length:812
    Mass (Da):91,546
    Last modified:November 1, 1997 - v2
    Checksum:i3C8653CF13D0F140
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti18 – 225DYLDF → RLLGL in CAA44079. (PubMed:1549468)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC031700 mRNA. Translation: AAH31700.1.
    X62154 mRNA. Translation: CAA44079.1.
    D26088 Genomic DNA. Translation: BAA05081.1.
    CCDSiCCDS14843.1.
    PIRiS22804.
    S51615.
    RefSeqiNP_032589.1. NM_008563.2.
    UniGeneiMm.4502.

    Genome annotation databases

    EnsembliENSMUST00000053266; ENSMUSP00000059192; ENSMUSG00000041859.
    GeneIDi17215.
    KEGGimmu:17215.
    UCSCiuc007alc.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC031700 mRNA. Translation: AAH31700.1 .
    X62154 mRNA. Translation: CAA44079.1 .
    D26088 Genomic DNA. Translation: BAA05081.1 .
    CCDSi CCDS14843.1.
    PIRi S22804.
    S51615.
    RefSeqi NP_032589.1. NM_008563.2.
    UniGenei Mm.4502.

    3D structure databases

    ProteinModelPortali P25206.
    SMRi P25206. Positions 10-650.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201345. 4 interactions.
    DIPi DIP-45878N.
    IntActi P25206. 1 interaction.
    MINTi MINT-4101604.

    PTM databases

    PhosphoSitei P25206.

    Proteomic databases

    MaxQBi P25206.
    PaxDbi P25206.
    PRIDEi P25206.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000053266 ; ENSMUSP00000059192 ; ENSMUSG00000041859 .
    GeneIDi 17215.
    KEGGi mmu:17215.
    UCSCi uc007alc.1. mouse.

    Organism-specific databases

    CTDi 4172.
    MGIi MGI:101845. Mcm3.

    Phylogenomic databases

    eggNOGi COG1241.
    HOGENOMi HOG000224126.
    HOVERGENi HBG104962.
    InParanoidi P25206.
    KOi K02541.
    OMAi RMISEHV.
    OrthoDBi EOG7V1FQ3.
    PhylomeDBi P25206.
    TreeFami TF106459.

    Enzyme and pathway databases

    Reactomei REACT_217374. Unwinding of DNA.

    Miscellaneous databases

    ChiTaRSi MCM3. mouse.
    NextBioi 291602.
    PMAP-CutDB P25206.
    PROi P25206.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P25206.
    Bgeei P25206.
    Genevestigatori P25206.

    Family and domain databases

    Gene3Di 2.40.50.140. 2 hits.
    3.40.50.300. 1 hit.
    InterProi IPR003593. AAA+_ATPase.
    IPR008046. Mcm3.
    IPR018525. MCM_CS.
    IPR001208. MCM_DNA-dep_ATPase.
    IPR027925. MCM_N.
    IPR012340. NA-bd_OB-fold.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00493. MCM. 1 hit.
    PF14551. MCM_N. 1 hit.
    [Graphical view ]
    PRINTSi PR01657. MCMFAMILY.
    PR01659. MCMPROTEIN3.
    SMARTi SM00382. AAA. 1 hit.
    SM00350. MCM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50249. SSF50249. 1 hit.
    SSF52540. SSF52540. 2 hits.
    PROSITEi PS00847. MCM_1. 1 hit.
    PS50051. MCM_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N-3.
      Tissue: Mammary gland.
    2. "Properties of the nuclear P1 protein, a mammalian homologue of the yeast Mcm3 replication protein."
      Thoemmes P., Fett R., Schray B., Burkhart R., Barnes M., Kennedy C., Brown N.C., Knippers R.
      Nucleic Acids Res. 20:1069-1074(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 18-812.
    3. "DNA polymerase alpha associated protein P1, a murine homolog of yeast MCM3, changes its intranuclear distribution during the DNA synthetic period."
      Kimura H., Nozaki N., Sugimoto K.
      EMBO J. 13:4311-4320(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-26.
    4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-668 AND SER-672, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    5. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
      Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
      Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-672, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.
    6. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-293 AND LYS-547, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiMCM3_MOUSE
    AccessioniPrimary (citable) accession number: P25206
    Secondary accession number(s): Q61492
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 135 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3