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P25206

- MCM3_MOUSE

UniProt

P25206 - MCM3_MOUSE

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Protein

DNA replication licensing factor MCM3

Gene

Mcm3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi345 – 3528ATPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA helicase activity Source: InterPro

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. DNA replication initiation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Cell cycle, DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_217374. Unwinding of DNA.
REACT_230841. Assembly of the pre-replicative complex.
REACT_232236. Activation of ATR in response to replication stress.
REACT_236939. Removal of licensing factors from origins.
REACT_245230. Orc1 removal from chromatin.
REACT_254983. Activation of the pre-replicative complex.
REACT_262530. Switching of origins to a post-replicative state.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor MCM3 (EC:3.6.4.12)
Alternative name(s):
DNA polymerase alpha holoenzyme-associated protein P1
P1-MCM3
Gene namesi
Name:Mcm3
Synonyms:Mcmd, Mcmd3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:101845. Mcm3.

Subcellular locationi

GO - Cellular componenti

  1. centrosome Source: Ensembl
  2. cytoplasm Source: MGI
  3. MCM complex Source: UniProtKB
  4. nucleus Source: MGI
  5. perinuclear region of cytoplasm Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 812811DNA replication licensing factor MCM3PRO_0000194094Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei160 – 1601PhosphoserineBy similarity
Modified residuei293 – 2931N6-acetyllysine1 Publication
Modified residuei547 – 5471N6-acetyllysine1 Publication
Modified residuei668 – 6681Phosphoserine1 Publication
Modified residuei672 – 6721Phosphoserine2 Publications
Modified residuei681 – 6811PhosphoserineBy similarity
Modified residuei705 – 7051PhosphotyrosineBy similarity
Modified residuei708 – 7081PhosphoserineBy similarity
Modified residuei719 – 7191PhosphothreonineBy similarity
Modified residuei722 – 7221PhosphothreonineBy similarity

Post-translational modificationi

O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.By similarity

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP25206.
PaxDbiP25206.
PRIDEiP25206.

PTM databases

PhosphoSiteiP25206.

Miscellaneous databases

PMAP-CutDBP25206.

Expressioni

Gene expression databases

BgeeiP25206.
ExpressionAtlasiP25206. baseline and differential.
GenevestigatoriP25206.

Interactioni

Subunit structurei

Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5. Associated with the replication-specific DNA polymerase alpha. Interacts with MCMBP (By simililarity).

Protein-protein interaction databases

BioGridi201345. 4 interactions.
DIPiDIP-45878N.
IntActiP25206. 1 interaction.
MINTiMINT-4101604.

Structurei

3D structure databases

ProteinModelPortaliP25206.
SMRiP25206. Positions 10-650.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini295 – 502208MCMAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi477 – 4804Arginine finger

Sequence similaritiesi

Belongs to the MCM family.Curated
Contains 1 MCM domain.Curated

Phylogenomic databases

eggNOGiCOG1241.
HOGENOMiHOG000224126.
HOVERGENiHBG104962.
InParanoidiP25206.
KOiK02541.
OMAiRMISEHV.
OrthoDBiEOG7V1FQ3.
PhylomeDBiP25206.
TreeFamiTF106459.

Family and domain databases

Gene3Di2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR008046. Mcm3.
IPR018525. MCM_CS.
IPR001208. MCM_DNA-dep_ATPase.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
PR01659. MCMPROTEIN3.
SMARTiSM00382. AAA. 1 hit.
SM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25206-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAGTVVLDDV ELREAQRDYL DFLDDEEDQG IYQNKVRELI SDNQYRLIVS
60 70 80 90 100
VNDLRRKNEK RANRLLNNAF EELVAFQRAL KDFVASIDAT YAKQYEEFYI
110 120 130 140 150
GLEGSFGSKH VSPRTLTSCF LSCVVCVEGI VTKCSLVRPK VVRSVHYCPA
160 170 180 190 200
TKKTIERRYS DLTTLVAFPS SSVYPTKDEE NNPLETEYGL SVYKDHQTIT
210 220 230 240 250
IQEMPEKAPA GQLPRSVDVI LDDDLVDKVK PGDRIQVVGT YRCLPGKKGC
260 270 280 290 300
YTSGTFRTVL IACNVKQMSK DIQPAFSADD IAKIKKFSKT RSKDVFEQLA
310 320 330 340 350
RSLAPSIHGH DYVKKAILCL LLGGVERELE NGSHIRGDIN ILLIGDPSVA
360 370 380 390 400
KSQLLRYVLC TAPRAIPTTG RGSSGVGLTA AVTTDQETGE RRLEAGAMVL
410 420 430 440 450
ADRGVVCIDE FDKMSDMDRT AIHEVMEQGR VTIAKAGIHA RLNARCSVLA
460 470 480 490 500
AANPVYGRYD QYKTPMENIG LQDSLLSRFD LLFIMLDQMD PEQDREISDH
510 520 530 540 550
VLRMHQYRAP GEQDGDALPL GSSVDILATD DPDFTQDDQQ DTRIYEKHDS
560 570 580 590 600
LLHGTKKKKE KMVSAAFMKK YIHVAKIIKP TLTQESAAYI AEEYSRLRSQ
610 620 630 640 650
DSMSSDTART SPVTARTLET LIRLATAHAK ARMSKTVDLQ DAEEAVELVQ
660 670 680 690 700
YAYFKKVLEK EKKRKKASED ESDLEDEEEK SQEDTEQKRK RRKTHAKDGE
710 720 730 740 750
SYDPYDFSEA ETQMPQVHTP KTDDSQEKTD DSQETQDSQK VELSEPRLKA
760 770 780 790 800
FKAALLEVFQ EAHEQSVGML HLTESINRNR EEPFSSEEIQ ACLSRMQDDN
810
QVMVSEGIVF LI
Length:812
Mass (Da):91,546
Last modified:November 1, 1997 - v2
Checksum:i3C8653CF13D0F140
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti18 – 225DYLDF → RLLGL in CAA44079. (PubMed:1549468)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC031700 mRNA. Translation: AAH31700.1.
X62154 mRNA. Translation: CAA44079.1.
D26088 Genomic DNA. Translation: BAA05081.1.
CCDSiCCDS14843.1.
PIRiS22804.
S51615.
RefSeqiNP_032589.1. NM_008563.2.
UniGeneiMm.4502.

Genome annotation databases

EnsembliENSMUST00000053266; ENSMUSP00000059192; ENSMUSG00000041859.
GeneIDi17215.
KEGGimmu:17215.
UCSCiuc007alc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC031700 mRNA. Translation: AAH31700.1 .
X62154 mRNA. Translation: CAA44079.1 .
D26088 Genomic DNA. Translation: BAA05081.1 .
CCDSi CCDS14843.1.
PIRi S22804.
S51615.
RefSeqi NP_032589.1. NM_008563.2.
UniGenei Mm.4502.

3D structure databases

ProteinModelPortali P25206.
SMRi P25206. Positions 10-650.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 201345. 4 interactions.
DIPi DIP-45878N.
IntActi P25206. 1 interaction.
MINTi MINT-4101604.

PTM databases

PhosphoSitei P25206.

Proteomic databases

MaxQBi P25206.
PaxDbi P25206.
PRIDEi P25206.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000053266 ; ENSMUSP00000059192 ; ENSMUSG00000041859 .
GeneIDi 17215.
KEGGi mmu:17215.
UCSCi uc007alc.1. mouse.

Organism-specific databases

CTDi 4172.
MGIi MGI:101845. Mcm3.

Phylogenomic databases

eggNOGi COG1241.
HOGENOMi HOG000224126.
HOVERGENi HBG104962.
InParanoidi P25206.
KOi K02541.
OMAi RMISEHV.
OrthoDBi EOG7V1FQ3.
PhylomeDBi P25206.
TreeFami TF106459.

Enzyme and pathway databases

Reactomei REACT_217374. Unwinding of DNA.
REACT_230841. Assembly of the pre-replicative complex.
REACT_232236. Activation of ATR in response to replication stress.
REACT_236939. Removal of licensing factors from origins.
REACT_245230. Orc1 removal from chromatin.
REACT_254983. Activation of the pre-replicative complex.
REACT_262530. Switching of origins to a post-replicative state.

Miscellaneous databases

ChiTaRSi Mcm3. mouse.
NextBioi 291602.
PMAP-CutDB P25206.
PROi P25206.
SOURCEi Search...

Gene expression databases

Bgeei P25206.
ExpressionAtlasi P25206. baseline and differential.
Genevestigatori P25206.

Family and domain databases

Gene3Di 2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProi IPR003593. AAA+_ATPase.
IPR008046. Mcm3.
IPR018525. MCM_CS.
IPR001208. MCM_DNA-dep_ATPase.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view ]
PRINTSi PR01657. MCMFAMILY.
PR01659. MCMPROTEIN3.
SMARTi SM00382. AAA. 1 hit.
SM00350. MCM. 1 hit.
[Graphical view ]
SUPFAMi SSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEi PS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N-3.
    Tissue: Mammary gland.
  2. "Properties of the nuclear P1 protein, a mammalian homologue of the yeast Mcm3 replication protein."
    Thoemmes P., Fett R., Schray B., Burkhart R., Barnes M., Kennedy C., Brown N.C., Knippers R.
    Nucleic Acids Res. 20:1069-1074(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 18-812.
  3. "DNA polymerase alpha associated protein P1, a murine homolog of yeast MCM3, changes its intranuclear distribution during the DNA synthetic period."
    Kimura H., Nozaki N., Sugimoto K.
    EMBO J. 13:4311-4320(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-26.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-668 AND SER-672, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  5. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-672, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  6. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-293 AND LYS-547, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiMCM3_MOUSE
AccessioniPrimary (citable) accession number: P25206
Secondary accession number(s): Q61492
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: November 1, 1997
Last modified: November 26, 2014
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3