Reviewed,
UniProtKB/Swiss-Prot P25202 (RPC1_GIALA)
Last modified
June 16, 2009.
Version 56.
History...
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: DNA-directed RNA polymerase III subunit RPC1 Short name=RNA polymerase III subunit C1 EC=2.7.7.6 Alternative name(s): DNA-directed RNA polymerase III largest subunit RNA polymerase III subunit C160 | ||
| Gene names |
| ||
| Organism | Giardia lamblia (Giardia intestinalis) | ||
| Taxonomic identifier | 5741 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Diplomonadida › Hexamitidae › Giardiinae › Giardia |
Protein attributes
| Sequence length | 1741 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition By similarity. |
| Catalytic activity | Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). |
| Subunit structure | Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits By similarity. |
| Subcellular location | Nucleus By similarity. |
| Sequence similarities | Belongs to the RNA polymerase beta' chain family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Transcription |
| Cellular component | DNA-directed RNA polymerase Nucleus |
| Domain | Zinc-finger |
| Ligand | Magnesium Metal-binding Zinc |
| Molecular function | Nucleotidyltransferase Transferase |
| Gene Ontology (GO) | |
| Biological process | transcription Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activityInferred from electronic annotation. Source: UniProtKB-KW magnesium ion bindingInferred from electronic annotation. Source: UniProtKB-KW ribonucleoside bindingInferred from electronic annotation. Source: InterPro zinc ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1741 | 1741 | DNA-directed RNA polymerase III subunit RPC1 | PRO_0000073948 | |||||
Regions | |||||||||
| Zinc finger | 79 – 92 | 14 | C3H1-type Potential | ||||||
| Region | 1099 – 1111 | 13 | Bridging helix By similarity | ||||||
Sites | |||||||||
| Metal binding | 79 | 1 | Zinc 1 By similarity | ||||||
| Metal binding | 82 | 1 | Zinc 1 By similarity | ||||||
| Metal binding | 89 | 1 | Zinc 1 By similarity | ||||||
| Metal binding | 92 | 1 | Zinc 1 By similarity | ||||||
| Metal binding | 119 | 1 | Zinc 2 By similarity | ||||||
| Metal binding | 122 | 1 | Zinc 2 By similarity | ||||||
| Metal binding | 160 | 1 | Zinc 2 By similarity | ||||||
| Metal binding | 722 | 1 | Magnesium; catalytic By similarity | ||||||
| Metal binding | 724 | 1 | Magnesium; catalytic By similarity | ||||||
| Metal binding | 726 | 1 | Magnesium; catalytic By similarity | ||||||
Sequences
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References
| [1] | "Nucleotide sequence of the gene encoding the largest subunit of the DNA-dependent RNA polymerase III of Giardia lamblia." Lanzendoerfer M., Palm P., Grampp B., Peattie D.A., Zillig W. Nucleic Acids Res. 20:1145-1145(1992) [PubMed: 1549483] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SSP. P1. |
Cross-references
Sequence databases | |
|---|---|
| X60325 Genomic DNA. Translation: CAA42895.1. | |
| PIR | S22812. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1I6H based on UniProtKB P04050. |
| ModBase | Search... |
Enzyme and pathway databases | |
| BRENDA | 2.7.7.6. 322. |
Family and domain databases | |
| InterPro | IPR015700. DNA-dir_RNA_pol3_lsu. IPR000722. RNA_pol_asu. IPR006592. RNA_pol_N. IPR007080. RNA_pol_Rpb1_1. IPR007066. RNA_pol_Rpb1_3. IPR007083. RNA_pol_Rpb1_4. IPR007081. RNA_pol_Rpb1_5. [Graphical view] |
| Gene3D | G3DSA:2.40.40.30. RNA_pol_A. 1 hit. G3DSA:3.90.1120.10. RNA_pol_Rpb1_1. 1 hit. |
| PANTHER | PTHR19376:SF15. RNA_pol3. 1 hit. |
| Pfam | PF04997. RNA_pol_Rpb1_1. 1 hit. PF00623. RNA_pol_Rpb1_2. 1 hit. PF04983. RNA_pol_Rpb1_3. 1 hit. PF05000. RNA_pol_Rpb1_4. 1 hit. PF04998. RNA_pol_Rpb1_5. 1 hit. [Graphical view] |
| SMART | SM00663. RPOLA_N. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | RPC1_GIALA | ||||||||
| Accession | Primary (citable) accession number: P25202 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
