Reviewed, UniProtKB/Swiss-Prot P25202 (RPC1_GIALA)

Names and origin

Protein names

Recommended name:
    DNA-directed RNA polymerase III subunit RPC1
      Short name=RNA polymerase III subunit C1
    EC=2.7.7.6
Alternative name(s):
    DNA-directed RNA polymerase III largest subunit
    RNA polymerase III subunit C160

Gene names

Name:

RPOA3

Organism

Giardia lamblia (Giardia intestinalis)

Taxonomic identifier

5741 [NCBI]

Taxonomic lineage

Eukaryota › Diplomonadida › Hexamitidae › Giardiinae › Giardia

Protein attributes

Sequence length	1741 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the RNA polymerase beta' chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase Nucleus
Ligand	Magnesium Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
Gene Ontology (GO)
Biological process	transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1741	1741	DNA-directed RNA polymerase III subunit RPC1		PRO_0000073948
Regions
	Region	1099 – 1111	13	Bridging helix By similarity
Sites
	Metal binding	79	1	Zinc 1 By similarity
	Metal binding	82	1	Zinc 1 By similarity
	Metal binding	89	1	Zinc 1 By similarity
	Metal binding	92	1	Zinc 1 By similarity
	Metal binding	119	1	Zinc 2 By similarity
	Metal binding	122	1	Zinc 2 By similarity
	Metal binding	160	1	Zinc 2 By similarity
	Metal binding	722	1	Magnesium; catalytic By similarity
	Metal binding	724	1	Magnesium; catalytic By similarity
	Metal binding	726	1	Magnesium; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	P25202-1 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: DE2EF212CBCF6CD8	FASTA	1,741	193,929
	10 20 30 40 50 60 MEPNRRLRYF RSEIPQPVTE HRFPKRVEKL VFSVLSSNAL MKLSEVQITT PILYEHYPSP 70 80 90 100 110 120 ATNGVLDKRL GLSDKVGKCA TCGKDTKMCI GHFGTISLAL PVFHPGLIND ARTMLSIVCP 130 140 150 160 170 180 FCSRVKISEA DRLKYKVLNH KFSLAIVKEA QEQAKKQVNC PFCHAPSTIC KKSQGFRVLR 190 200 210 220 230 240 EMHYEERATK ILADVIKHQK DIEKIQELDQ RLRNHVVDPI QALHILQKVP ECDYPYLGIP 250 260 270 280 290 300 WQSRNVYASQ QTPDSIASND ASQLFSRGSS KLLARTASRF IAQHQGISDR ATRLTSHAFL 310 320 330 340 350 360 TRPQDVILTH IPVPPSCLRP SVQSGSGAGT TEDNLTSHLV RILTINDKLK EAMVSGAVAT 370 380 390 400 410 420 SKVYAKWDQL NMDVSLLYIG KIPGGVQPVK NKTDSGTKEG TGIIDRLKGK AGRFRSNLSG 430 440 450 460 470 480 KRVNFSGRTV ISPNPYLSMQ QVAIPRLIAQ NLTFPEIVTS RNIRFLRELI LNGKTYPGAN 490 500 510 520 530 540 EVLLLNDTIR YNPALKRLGI DVKQSRDMEA KAMELVSRCV DTFNLNSLCA DLPELQAAIQ 550 560 570 580 590 600 HLVSESSNAI NSELKAEDLP QNQPLFAKEV YDPAQKGESN NIDNNVCEID ELKLEDTADY 610 620 630 640 650 660 QVGESAGQAS TVAVEQPPES RTVLKLNSIA SLQLTNASSQ HLSTFKLNTV SLLQKDRRGR 670 680 690 700 710 720 QAIANSLRPN DIVYRHLLPN DTLLFNRQPS LHRMSIMQFN AVIHENRTFS FNPVVCSPFN 730 740 750 760 770 780 ADFDGDEMNI FYMQGQEARA EAGILMGSHE NIISPRHGEC MIGLTQDFLT GIYLLSGKGI 790 800 810 820 830 840 FMTRQEYCQH VSYGCDGFGD ATYGVSLYNY GREFIKSIHD RRGTEEDTVG FVKVPCLSQP 850 860 870 880 890 900 CIVYPRQLYS GKQVLSVLMK GNDFDSVNIN LEHGDKTYKK DSDRRALSVN DDYIIIQNSE 910 920 930 940 950 960 HLVGRLTKTF LASSKNCIFY FLVQNYGPVS AARIMLRFAK VAARFLMNYG FTIGIDDVMP 970 980 990 1000 1010 1020 SQRVLGKKEV IVQEGYEKAQ EKIRDYESGK LEAIPGSTVQ ETLEATLNQI LSNVRESCAQ 1030 1040 1050 1060 1070 1080 IALKELHFTN KPLIMSLCGS KGSPINIAQM IIILGQQSFG GSRAPDDFYT RSTPYFYHYS 1090 1100 1110 1120 1130 1140 KEPNAKGFIL NSFYTGLLPF EFLAHARAGR DGVIDSACKT ADTGYLQRRL VKCLEDLSVS 1150 1160 1170 1180 1190 1200 YDFTVRNSKK SVVQFRFGHD GFDPIKLEVG TGQCFDLDTL LRHIILLNRS QDLAEGAFPV 1210 1220 1230 1240 1250 1260 LLDRYKAEAE LDVLLNKDFY GQEACLIERS NVTSYFQQSI LPKLTTNTHI ATTVAPALAA 1270 1280 1290 1300 1310 1320 SFLQTTLTKK DIDDFLRQVR RKYIRLNLEP GSPCGAVAAQ SVGEPSTQMT LKSFHHAGLA 1330 1340 1350 1360 1370 1380 SMNITQGVPR LKEIVDGVVK ISTPITTVEL HVDVDKEELA ATVTEKYLEK ARMMKNIIEC 1390 1400 1410 1420 1430 1440 TYLGQISASI IECYSQSVCH IEVNLDMKII ADMGLAGIIT VETVVASILH NDKAKKLVGQ 1450 1460 1470 1480 1490 1500 DQSSGSVSIH SATRFSVRPL TQSRDDLLFD IQSLKLLLPM IPVSGISTCS RAVINEYKEF 1510 1520 1530 1540 1550 1560 CQGATSSAPL TKYNLLVEGI GLQNILNVSG IDFTRTLSNN IVEVANTLGI EAAVATISNE 1570 1580 1590 1600 1610 1620 IKACMDSHGM AVDMRHIRLL ADIMCFRGRV LGFTRFGLTK MKADSVIMLA SFEKTGEHLF 1630 1640 1650 1660 1670 1680 NAALGNKVDE ANGVTESIIL GKPMSMGTGS FSLLQAPYFD EKTGKTIEYQ PKQTTRFLGE 1690 1700 1710 1720 1730 1740 VLNKQYDEEV DAIVNAFWYD EKLYLTGAEA KAKLLRGRRH ANKRSWSRGK ERHASLKPKN R

References

[1]

"Nucleotide sequence of the gene encoding the largest subunit of the DNA-dependent RNA polymerase III of Giardia lamblia." Lanzendoerfer M., Palm P., Grampp B., Peattie D.A., Zillig W. Nucleic Acids Res. 20:1145-1145(1992) [PubMed: 1549483] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SSP. P1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X60325 Genomic DNA. Translation: CAA42895.1.
PIR	S22812.
3D structure databases
SMR	P25202. Positions 1267-1658.
ModBase	Search...
Phylogenomic databases
OMA	HENIISP.
Enzyme and pathway databases
BRENDA	2.7.7.6. 322.
Family and domain databases
InterPro	IPR015700. DNA-dir_RNA_pol3_lsu. IPR000722. RNA_pol_asu. IPR006592. RNA_pol_N. IPR007080. RNA_pol_Rpb1_1. IPR007066. RNA_pol_Rpb1_3. IPR007083. RNA_pol_Rpb1_4. IPR007081. RNA_pol_Rpb1_5. [Graphical view]
Gene3D	G3DSA:2.40.40.30. RNA_pol_A. 1 hit. G3DSA:3.90.1120.10. RNA_pol_Rpb1_1. 1 hit.
PANTHER	PTHR19376:SF15. RNA_pol3. 1 hit.
Pfam	PF04997. RNA_pol_Rpb1_1. 2 hits. PF00623. RNA_pol_Rpb1_2. 2 hits. PF04983. RNA_pol_Rpb1_3. 1 hit. PF05000. RNA_pol_Rpb1_4. 1 hit. PF04998. RNA_pol_Rpb1_5. 1 hit. [Graphical view]
SMART	SM00663. RPOLA_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPC1_GIALA

Accession

Primary (citable) accession number: P25202

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	January 19, 2010
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)