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P25201

- MTA1_ACICA

UniProt

P25201 - MTA1_ACICA

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Protein

Modification methylase AccI

Gene
accIM
Organism
Acinetobacter calcoaceticus
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GTMKAC, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the AccI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3271. M.AccI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase AccI (EC:2.1.1.72)
Short name:
M.AccI
Alternative name(s):
Adenine-specific methyltransferase AccI
Gene namesi
Name:accIM
OrganismiAcinetobacter calcoaceticus
Taxonomic identifieri471 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacterAcinetobacter calcoaceticus/baumannii complex

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 540540Modification methylase AccIPRO_0000087941Add
BLAST

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP25201.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR011639. RM_methylase_Eco57I.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF07669. Eco57I. 1 hit.
[Graphical view]
PRINTSiPR00507. N12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25201-1 [UniParc]FASTAAdd to Basket

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MIKTTEHIVN NSIERDYSKS ISLEHRKKFA QFFTPFPIAY AMAKWILGNK    50
QLKTVLEPAF GLGVFSRAIL SQQKEINIKA FEVDETIFEN AKEYFDDFEN 100
VNILLQDYMY NDWKNKYDGI ICNPPYFKFH DYDNKNILKE IETNLKCKLN 150
GFTNLYTLFL LKSIHQLSQN GRCAYIIPSE FLNSDYGKLV KTYLIKSKTL 200
RHIIVIDFEE NVFDDALTTA SIILCANDNI TDKVQFNNIQ SLQDLSKIDE 250
IINKYPNFLE TEQTYNFSDL NPEIKWKAYY QKQNSIKFKN LVPFSTYAKV 300
VRGIATGSNE YFTFNLSKAK EFNIDEQYLL PCICSAKDAK TSFFTKQDFE 350
ELKKSDKSVF LFNAQNSTDK NISSYIQKGE SEEINKRFLT ASRTPWYSLE 400
NRKPAPIWVS VFNRSGLRFI RNEANISNLT SYHCIIQNKQ VVSEIDIDLL 450
FAYLLTDTAK QIFEDNSRQY GNGLQKFEPN DLNKGMMLDL GLLDKQTSDE 500
ILNLYKEYKY LILDNKNGDE IINKIDKILT DKYSEKKHWA 540
Length:540
Mass (Da):63,093
Last modified:July 1, 1993 - v2
Checksum:iA2569693712E0F59
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10671 Genomic DNA. Translation: BAA01523.1.
PIRiJU0470.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10671 Genomic DNA. Translation: BAA01523.1 .
PIRi JU0470.

3D structure databases

ProteinModelPortali P25201.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3271. M.AccI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR011639. RM_methylase_Eco57I.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF07669. Eco57I. 1 hit.
[Graphical view ]
PRINTSi PR00507. N12N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and nucleotide sequences of the AccI restriction-modification genes in Acinetobacter calcoaceticus."
    Kawakami B., Hilzheber C., Nagatomo M., Oka M.
    Agric. Biol. Chem. 55:1553-1559(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 49823.

Entry informationi

Entry nameiMTA1_ACICA
AccessioniPrimary (citable) accession number: P25201
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: July 1, 1993
Last modified: June 11, 2014
This is version 67 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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