Reviewed, UniProtKB/Swiss-Prot P25201 (MTA1_ACICA)

Names and origin

Protein names

Recommended name:
    Modification methylase AccI
      Short name=M.AccI
    EC=2.1.1.72
Alternative name(s):
    Adenine-specific methyltransferase AccI

Gene names

Name:

accIM

Organism

Acinetobacter calcoaceticus

Taxonomic identifier

471 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	540 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GTMKAC, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the AccI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Subunit structure	Monomer.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW methylation Inferred from electronic annotation. Source: InterPro
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 540	540	Modification methylase AccI		PRO_0000087941

Sequences

	Sequence		Length	Mass (Da)
	P25201-1 [UniParc]. Last modified July 1, 1993. Version 2. Checksum: A2569693712E0F59	FASTA	540	63,093
	10 20 30 40 50 60 MIKTTEHIVN NSIERDYSKS ISLEHRKKFA QFFTPFPIAY AMAKWILGNK QLKTVLEPAF 70 80 90 100 110 120 GLGVFSRAIL SQQKEINIKA FEVDETIFEN AKEYFDDFEN VNILLQDYMY NDWKNKYDGI 130 140 150 160 170 180 ICNPPYFKFH DYDNKNILKE IETNLKCKLN GFTNLYTLFL LKSIHQLSQN GRCAYIIPSE 190 200 210 220 230 240 FLNSDYGKLV KTYLIKSKTL RHIIVIDFEE NVFDDALTTA SIILCANDNI TDKVQFNNIQ 250 260 270 280 290 300 SLQDLSKIDE IINKYPNFLE TEQTYNFSDL NPEIKWKAYY QKQNSIKFKN LVPFSTYAKV 310 320 330 340 350 360 VRGIATGSNE YFTFNLSKAK EFNIDEQYLL PCICSAKDAK TSFFTKQDFE ELKKSDKSVF 370 380 390 400 410 420 LFNAQNSTDK NISSYIQKGE SEEINKRFLT ASRTPWYSLE NRKPAPIWVS VFNRSGLRFI 430 440 450 460 470 480 RNEANISNLT SYHCIIQNKQ VVSEIDIDLL FAYLLTDTAK QIFEDNSRQY GNGLQKFEPN 490 500 510 520 530 540 DLNKGMMLDL GLLDKQTSDE ILNLYKEYKY LILDNKNGDE IINKIDKILT DKYSEKKHWA

References

[1]	"Cloning and nucleotide sequences of the AccI restriction-modification genes in Acinetobacter calcoaceticus." Kawakami B., Hilzheber C., Nagatomo M., Oka M. Agric. Biol. Chem. 55:1553-1559(1991) [PubMed: 1368703] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 49823.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D10671 Genomic DNA. Translation: BAA01523.1.
PIR	JU0470.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	3271. M.AccI.
Enzyme and pathway databases
BRENDA	2.1.1.72. 412.
Family and domain databases
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR002296. N12N6_MeTrfase. [Graphical view]
PRINTS	PR00507. N12N6MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTA1_ACICA

Accession

Primary (citable) accession number: P25201

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	July 1, 1993
Last modified:	September 22, 2009
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)