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P25201

- MTA1_ACICA

UniProt

P25201 - MTA1_ACICA

Protein

Modification methylase AccI

Gene

accIM

Organism
Acinetobacter calcoaceticus
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 2 (01 Jul 1993)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence GTMKAC, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the AccI endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3271. M.AccI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase AccI (EC:2.1.1.72)
    Short name:
    M.AccI
    Alternative name(s):
    Adenine-specific methyltransferase AccI
    Gene namesi
    Name:accIM
    OrganismiAcinetobacter calcoaceticus
    Taxonomic identifieri471 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacterAcinetobacter calcoaceticus/baumannii complex

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 540540Modification methylase AccIPRO_0000087941Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.

    Structurei

    3D structure databases

    ProteinModelPortaliP25201.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR002052. DNA_methylase_N6_adenine_CS.
    IPR002296. N12N6_MeTrfase.
    IPR011639. RM_methylase_Eco57I.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF07669. Eco57I. 1 hit.
    [Graphical view]
    PRINTSiPR00507. N12N6MTFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS00092. N6_MTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P25201-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIKTTEHIVN NSIERDYSKS ISLEHRKKFA QFFTPFPIAY AMAKWILGNK    50
    QLKTVLEPAF GLGVFSRAIL SQQKEINIKA FEVDETIFEN AKEYFDDFEN 100
    VNILLQDYMY NDWKNKYDGI ICNPPYFKFH DYDNKNILKE IETNLKCKLN 150
    GFTNLYTLFL LKSIHQLSQN GRCAYIIPSE FLNSDYGKLV KTYLIKSKTL 200
    RHIIVIDFEE NVFDDALTTA SIILCANDNI TDKVQFNNIQ SLQDLSKIDE 250
    IINKYPNFLE TEQTYNFSDL NPEIKWKAYY QKQNSIKFKN LVPFSTYAKV 300
    VRGIATGSNE YFTFNLSKAK EFNIDEQYLL PCICSAKDAK TSFFTKQDFE 350
    ELKKSDKSVF LFNAQNSTDK NISSYIQKGE SEEINKRFLT ASRTPWYSLE 400
    NRKPAPIWVS VFNRSGLRFI RNEANISNLT SYHCIIQNKQ VVSEIDIDLL 450
    FAYLLTDTAK QIFEDNSRQY GNGLQKFEPN DLNKGMMLDL GLLDKQTSDE 500
    ILNLYKEYKY LILDNKNGDE IINKIDKILT DKYSEKKHWA 540
    Length:540
    Mass (Da):63,093
    Last modified:July 1, 1993 - v2
    Checksum:iA2569693712E0F59
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10671 Genomic DNA. Translation: BAA01523.1.
    PIRiJU0470.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10671 Genomic DNA. Translation: BAA01523.1 .
    PIRi JU0470.

    3D structure databases

    ProteinModelPortali P25201.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3271. M.AccI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR002052. DNA_methylase_N6_adenine_CS.
    IPR002296. N12N6_MeTrfase.
    IPR011639. RM_methylase_Eco57I.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF07669. Eco57I. 1 hit.
    [Graphical view ]
    PRINTSi PR00507. N12N6MTFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS00092. N6_MTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and nucleotide sequences of the AccI restriction-modification genes in Acinetobacter calcoaceticus."
      Kawakami B., Hilzheber C., Nagatomo M., Oka M.
      Agric. Biol. Chem. 55:1553-1559(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 49823.

    Entry informationi

    Entry nameiMTA1_ACICA
    AccessioniPrimary (citable) accession number: P25201
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: July 1, 1993
    Last modified: October 1, 2014
    This is version 68 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3