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Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing]

Gene

nodM

Organism
Rhizobium meliloti (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the production of the root hair deformation (HAD) factor specifically on medicago.

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2Nucleophile; for GATase activityBy similarity1
Active sitei600For Fru-6P isomerization activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminotransferase, Transferase
Biological processNodulation

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC:2.6.1.16)
Short name:
GFAT
Alternative name(s):
Nodulation protein M
Gene namesi
Name:nodM
Encoded oniPlasmid pSymA (megaplasmid 1)0 Publication
OrganismiRhizobium meliloti (Ensifer meliloti) (Sinorhizobium meliloti)
Taxonomic identifieri382 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium

Subcellular locationi

P25195:

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001354382 – 605Glutamine--fructose-6-phosphate aminotransferase [isomerizing]Add BLAST604

Expressioni

Inductioni

In response to a plant signal such as luteolin via the regulatory gene NodD.

Structurei

3D structure databases

ProteinModelPortaliP25195.
SMRiP25195.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 216Glutamine amidotransferase type-2Add BLAST215
Domaini280 – 420SIS 1Add BLAST141
Domaini454 – 595SIS 2Add BLAST142

Keywords - Domaini

Glutamine amidotransferase, Repeat

Family and domain databases

CDDicd05008. SIS_GlmS_GlmD_1. 1 hit.
cd05009. SIS_GlmS_GlmD_2. 1 hit.
Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_00164. GlmS. 1 hit.
InterProiView protein in InterPro
IPR017932. GATase_2_dom.
IPR035466. GlmS/AgaS_SIS.
IPR035490. GlmS/FrlB_SIS.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
PfamiView protein in Pfam
PF01380. SIS. 2 hits.
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiView protein in PROSITE
PS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25195-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGIVGIVGH QPVSERLVEA LEPLEYRGYD SAGVATMDAG TLQRRRAEGK
60 70 80 90 100
LGNLREKLKE APLSGTIGIA HTRWATHGAP TERNAHPHFT EGVAVVHNGI
110 120 130 140 150
IENFAELKDE LAAGGAEFQT ETDTEVVAHL LAKYRRDGLG RREAMHAMLK
160 170 180 190 200
RVKGAYALAV LFEDDPSTIM AARTGPLAIG HGNGEMFLGS DAIALAPFTN
210 220 230 240 250
EITYLIDGDW AVIGKTGVHI FDFDGNVVER PRQISTAAAF LVDKGNHRHF
260 270 280 290 300
MEKEIYEQPE VIAIALGHYV NVIDKSCRSD SDAIDFAGVE SLAISCCGTA
310 320 330 340 350
YLAGLIGKYW FERYARLPVE IAVASEFRYR EIPLSPQSAL FISQSGETAD
360 370 380 390 400
TLASLRYCKA HGLRIGAVVN ARESTMARES DAVFPILAGP EIGVARTKAF
410 420 430 440 450
TCQLAVLAAL RAGAGKARGT ISGDEEQALI KSLAEMPAIM GQVLNSIQPE
460 470 480 490 500
IEVLSRELSN CRDVLYLGRG TSFPLAMEGA LKLKEISYIQ PKSYAAGQLK
510 520 530 540 550
HGPYALIDEN MPVIVIAPHD RFFDKTVTNM QEVARGGRII LITDEKGAAA
560 570 580 590 600
SKLDTMHTIV LPEVDEIIAP MIFSLPLQLL AYHTAVFMGT DVDQPRNLAK

SVTVE
Length:605
Mass (Da):65,606
Last modified:January 23, 2007 - v3
Checksum:iCA21C431400AC731
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58632 Genomic DNA. Translation: CAA41485.1.
PIRiS16561.

Similar proteinsi

Entry informationi

Entry nameiNODM_RHIML
AccessioniPrimary (citable) accession number: P25195
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2007
Last modified: October 25, 2017
This is version 90 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid