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P25195

- NODM_RHIML

UniProt

P25195 - NODM_RHIML

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Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing]

Gene

nodM

Organism
Rhizobium meliloti (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Involved in the production of the root hair deformation (HAD) factor specifically on medicago.

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Nucleophile; for GATase activityBy similarity
Active sitei600 – 6001For Fru-6P isomerization activityBy similarity

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. glutamine-fructose-6-phosphate transaminase (isomerizing) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate biosynthetic process Source: InterPro
  2. glutamine metabolic process Source: UniProtKB-HAMAP
  3. nodulation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Biological processi

Nodulation

Protein family/group databases

MEROPSiC44.971.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC:2.6.1.16)
Short name:
GFAT
Alternative name(s):
Nodulation protein M
Gene namesi
Name:nodM
Encoded oniPlasmid pSymA (megaplasmid 1)0 Publication
OrganismiRhizobium meliloti (Ensifer meliloti) (Sinorhizobium meliloti)
Taxonomic identifieri382 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 605604Glutamine--fructose-6-phosphate aminotransferase [isomerizing]PRO_0000135438Add
BLAST

Expressioni

Inductioni

In response to a plant signal such as luteolin via the regulatory gene NodD.

Structurei

3D structure databases

ProteinModelPortaliP25195.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 216215Glutamine amidotransferase type-2Add
BLAST
Domaini280 – 420141SIS 1Add
BLAST
Domaini454 – 595142SIS 2Add
BLAST

Sequence similaritiesi

Contains 2 SIS domains.Curated

Keywords - Domaini

Glutamine amidotransferase, Repeat

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_00164. GlmS.
InterProiIPR017932. GATase_2_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
PfamiPF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25195-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCGIVGIVGH QPVSERLVEA LEPLEYRGYD SAGVATMDAG TLQRRRAEGK
60 70 80 90 100
LGNLREKLKE APLSGTIGIA HTRWATHGAP TERNAHPHFT EGVAVVHNGI
110 120 130 140 150
IENFAELKDE LAAGGAEFQT ETDTEVVAHL LAKYRRDGLG RREAMHAMLK
160 170 180 190 200
RVKGAYALAV LFEDDPSTIM AARTGPLAIG HGNGEMFLGS DAIALAPFTN
210 220 230 240 250
EITYLIDGDW AVIGKTGVHI FDFDGNVVER PRQISTAAAF LVDKGNHRHF
260 270 280 290 300
MEKEIYEQPE VIAIALGHYV NVIDKSCRSD SDAIDFAGVE SLAISCCGTA
310 320 330 340 350
YLAGLIGKYW FERYARLPVE IAVASEFRYR EIPLSPQSAL FISQSGETAD
360 370 380 390 400
TLASLRYCKA HGLRIGAVVN ARESTMARES DAVFPILAGP EIGVARTKAF
410 420 430 440 450
TCQLAVLAAL RAGAGKARGT ISGDEEQALI KSLAEMPAIM GQVLNSIQPE
460 470 480 490 500
IEVLSRELSN CRDVLYLGRG TSFPLAMEGA LKLKEISYIQ PKSYAAGQLK
510 520 530 540 550
HGPYALIDEN MPVIVIAPHD RFFDKTVTNM QEVARGGRII LITDEKGAAA
560 570 580 590 600
SKLDTMHTIV LPEVDEIIAP MIFSLPLQLL AYHTAVFMGT DVDQPRNLAK

SVTVE
Length:605
Mass (Da):65,606
Last modified:January 23, 2007 - v3
Checksum:iCA21C431400AC731
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58632 Genomic DNA. Translation: CAA41485.1.
PIRiS16561.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58632 Genomic DNA. Translation: CAA41485.1 .
PIRi S16561.

3D structure databases

ProteinModelPortali P25195.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C44.971.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.60.20.10. 1 hit.
HAMAPi MF_00164. GlmS.
InterProi IPR017932. GATase_2_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view ]
PANTHERi PTHR10937:SF0. PTHR10937:SF0. 1 hit.
Pfami PF01380. SIS. 2 hits.
[Graphical view ]
SUPFAMi SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01135. glmS. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Six nodulation genes of nod box locus 4 in Rhizobium meliloti are involved in nodulation signal production: nodM codes for D-glucosamine synthetase."
    Baev N., Endre G., Petrovics G., Banfalvi Z., Kondorosi A.
    Mol. Gen. Genet. 228:113-124(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: AK631.

Entry informationi

Entry nameiNODM_RHIML
AccessioniPrimary (citable) accession number: P25195
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 80 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3