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P25195

- NODM_RHIML

UniProt

P25195 - NODM_RHIML

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Protein
Glutamine--fructose-6-phosphate aminotransferase [isomerizing]
Gene
nodM
Organism
Rhizobium meliloti (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Involved in the production of the root hair deformation (HAD) factor specifically on medicago.UniRule annotation

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Nucleophile; for GATase activity By similarity
Active sitei600 – 6001For Fru-6P isomerization activity By similarity

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. glutamine-fructose-6-phosphate transaminase (isomerizing) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate biosynthetic process Source: InterPro
  2. glutamine metabolic process Source: UniProtKB-HAMAP
  3. nodulation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Biological processi

Nodulation

Protein family/group databases

MEROPSiC44.971.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC:2.6.1.16)
Short name:
GFAT
Alternative name(s):
Nodulation protein M
Gene namesi
Name:nodM
Encoded oniPlasmid pSymA (megaplasmid 1)0 Publication
OrganismiRhizobium meliloti (Ensifer meliloti) (Sinorhizobium meliloti)
Taxonomic identifieri382 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 605604Glutamine--fructose-6-phosphate aminotransferase [isomerizing]UniRule annotation
PRO_0000135438Add
BLAST

Expressioni

Inductioni

In response to a plant signal such as luteolin via the regulatory gene NodD.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP25195.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 216215Glutamine amidotransferase type-2
Add
BLAST
Domaini280 – 420141SIS 1
Add
BLAST
Domaini454 – 595142SIS 2
Add
BLAST

Sequence similaritiesi

Contains 2 SIS domains.

Keywords - Domaini

Glutamine amidotransferase, Repeat

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_00164. GlmS.
InterProiIPR017932. GATase_2_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
PfamiPF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25195-1 [UniParc]FASTAAdd to Basket

« Hide

MCGIVGIVGH QPVSERLVEA LEPLEYRGYD SAGVATMDAG TLQRRRAEGK    50
LGNLREKLKE APLSGTIGIA HTRWATHGAP TERNAHPHFT EGVAVVHNGI 100
IENFAELKDE LAAGGAEFQT ETDTEVVAHL LAKYRRDGLG RREAMHAMLK 150
RVKGAYALAV LFEDDPSTIM AARTGPLAIG HGNGEMFLGS DAIALAPFTN 200
EITYLIDGDW AVIGKTGVHI FDFDGNVVER PRQISTAAAF LVDKGNHRHF 250
MEKEIYEQPE VIAIALGHYV NVIDKSCRSD SDAIDFAGVE SLAISCCGTA 300
YLAGLIGKYW FERYARLPVE IAVASEFRYR EIPLSPQSAL FISQSGETAD 350
TLASLRYCKA HGLRIGAVVN ARESTMARES DAVFPILAGP EIGVARTKAF 400
TCQLAVLAAL RAGAGKARGT ISGDEEQALI KSLAEMPAIM GQVLNSIQPE 450
IEVLSRELSN CRDVLYLGRG TSFPLAMEGA LKLKEISYIQ PKSYAAGQLK 500
HGPYALIDEN MPVIVIAPHD RFFDKTVTNM QEVARGGRII LITDEKGAAA 550
SKLDTMHTIV LPEVDEIIAP MIFSLPLQLL AYHTAVFMGT DVDQPRNLAK 600
SVTVE 605
Length:605
Mass (Da):65,606
Last modified:January 23, 2007 - v3
Checksum:iCA21C431400AC731
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X58632 Genomic DNA. Translation: CAA41485.1.
PIRiS16561.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X58632 Genomic DNA. Translation: CAA41485.1 .
PIRi S16561.

3D structure databases

ProteinModelPortali P25195.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C44.971.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.60.20.10. 1 hit.
HAMAPi MF_00164. GlmS.
InterProi IPR017932. GATase_2_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view ]
PANTHERi PTHR10937:SF0. PTHR10937:SF0. 1 hit.
Pfami PF01380. SIS. 2 hits.
[Graphical view ]
SUPFAMi SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01135. glmS. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Six nodulation genes of nod box locus 4 in Rhizobium meliloti are involved in nodulation signal production: nodM codes for D-glucosamine synthetase."
    Baev N., Endre G., Petrovics G., Banfalvi Z., Kondorosi A.
    Mol. Gen. Genet. 228:113-124(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: AK631.

Entry informationi

Entry nameiNODM_RHIML
AccessioniPrimary (citable) accession number: P25195
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 78 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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