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P25195

- NODM_RHIML

UniProt

P25195 - NODM_RHIML

Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing]

Gene

nodM

Organism
Rhizobium meliloti (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Involved in the production of the root hair deformation (HAD) factor specifically on medicago.

    Catalytic activityi

    L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21Nucleophile; for GATase activityBy similarity
    Active sitei600 – 6001For Fru-6P isomerization activityBy similarity

    GO - Molecular functioni

    1. carbohydrate binding Source: InterPro
    2. glutamine-fructose-6-phosphate transaminase (isomerizing) activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. carbohydrate biosynthetic process Source: InterPro
    2. glutamine metabolic process Source: UniProtKB-HAMAP
    3. nodulation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Aminotransferase, Transferase

    Keywords - Biological processi

    Nodulation

    Protein family/group databases

    MEROPSiC44.971.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC:2.6.1.16)
    Short name:
    GFAT
    Alternative name(s):
    Nodulation protein M
    Gene namesi
    Name:nodM
    Encoded oniPlasmid pSymA (megaplasmid 1)0 Publication
    OrganismiRhizobium meliloti (Ensifer meliloti) (Sinorhizobium meliloti)
    Taxonomic identifieri382 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 605604Glutamine--fructose-6-phosphate aminotransferase [isomerizing]PRO_0000135438Add
    BLAST

    Expressioni

    Inductioni

    In response to a plant signal such as luteolin via the regulatory gene NodD.

    Structurei

    3D structure databases

    ProteinModelPortaliP25195.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 216215Glutamine amidotransferase type-2Add
    BLAST
    Domaini280 – 420141SIS 1Add
    BLAST
    Domaini454 – 595142SIS 2Add
    BLAST

    Sequence similaritiesi

    Contains 2 SIS domains.Curated

    Keywords - Domaini

    Glutamine amidotransferase, Repeat

    Family and domain databases

    Gene3Di3.60.20.10. 1 hit.
    HAMAPiMF_00164. GlmS.
    InterProiIPR017932. GATase_2_dom.
    IPR005855. GlmS_trans.
    IPR029055. Ntn_hydrolases_N.
    IPR001347. SIS.
    [Graphical view]
    PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
    PfamiPF01380. SIS. 2 hits.
    [Graphical view]
    SUPFAMiSSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR01135. glmS. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    PS51464. SIS. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P25195-1 [UniParc]FASTAAdd to Basket

    « Hide

    MCGIVGIVGH QPVSERLVEA LEPLEYRGYD SAGVATMDAG TLQRRRAEGK    50
    LGNLREKLKE APLSGTIGIA HTRWATHGAP TERNAHPHFT EGVAVVHNGI 100
    IENFAELKDE LAAGGAEFQT ETDTEVVAHL LAKYRRDGLG RREAMHAMLK 150
    RVKGAYALAV LFEDDPSTIM AARTGPLAIG HGNGEMFLGS DAIALAPFTN 200
    EITYLIDGDW AVIGKTGVHI FDFDGNVVER PRQISTAAAF LVDKGNHRHF 250
    MEKEIYEQPE VIAIALGHYV NVIDKSCRSD SDAIDFAGVE SLAISCCGTA 300
    YLAGLIGKYW FERYARLPVE IAVASEFRYR EIPLSPQSAL FISQSGETAD 350
    TLASLRYCKA HGLRIGAVVN ARESTMARES DAVFPILAGP EIGVARTKAF 400
    TCQLAVLAAL RAGAGKARGT ISGDEEQALI KSLAEMPAIM GQVLNSIQPE 450
    IEVLSRELSN CRDVLYLGRG TSFPLAMEGA LKLKEISYIQ PKSYAAGQLK 500
    HGPYALIDEN MPVIVIAPHD RFFDKTVTNM QEVARGGRII LITDEKGAAA 550
    SKLDTMHTIV LPEVDEIIAP MIFSLPLQLL AYHTAVFMGT DVDQPRNLAK 600
    SVTVE 605
    Length:605
    Mass (Da):65,606
    Last modified:January 23, 2007 - v3
    Checksum:iCA21C431400AC731
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X58632 Genomic DNA. Translation: CAA41485.1.
    PIRiS16561.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X58632 Genomic DNA. Translation: CAA41485.1 .
    PIRi S16561.

    3D structure databases

    ProteinModelPortali P25195.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C44.971.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.60.20.10. 1 hit.
    HAMAPi MF_00164. GlmS.
    InterProi IPR017932. GATase_2_dom.
    IPR005855. GlmS_trans.
    IPR029055. Ntn_hydrolases_N.
    IPR001347. SIS.
    [Graphical view ]
    PANTHERi PTHR10937:SF0. PTHR10937:SF0. 1 hit.
    Pfami PF01380. SIS. 2 hits.
    [Graphical view ]
    SUPFAMi SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR01135. glmS. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    PS51464. SIS. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Six nodulation genes of nod box locus 4 in Rhizobium meliloti are involved in nodulation signal production: nodM codes for D-glucosamine synthetase."
      Baev N., Endre G., Petrovics G., Banfalvi Z., Kondorosi A.
      Mol. Gen. Genet. 228:113-124(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: AK631.

    Entry informationi

    Entry nameiNODM_RHIML
    AccessioniPrimary (citable) accession number: P25195
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 79 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Plasmid

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3