P25188 (MBP_CAVPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Myelin basic protein
Short name=MBP

Gene names

Name:

MBP

Organism

Cavia porcellus (Guinea pig)

Taxonomic identifier

10141 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Hystricognathi › Caviidae › Cavia

Protein attributes

Sequence length	167 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Is, with PLP, the most abundant protein component of the myelin membrane in the CNS. Has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. Each splice variant and charge isomer may have a specialized function in the assembly of an optimized, biochemically functional myelin membrane By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Myelin membrane; Peripheral membrane protein; Cytoplasmic side. Note: Cytoplasmic side of myelin.
Tissue specificity	Found in both the central and the peripheral nervous system.
Post-translational modification	At least 5 charge isomers; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4 and C5 (the least cationic form); are produced as a result of optional post-translational modifications such as phosphorylation of serine or threonine residues, deamidation of glutamine or asparagine residues, citrullination and methylation of arginine residues. C1 and C2 are unphosphorylated, C3 and C4 are monophosphorylated and C5 is phosphorylated at two positions. Phosphorylated by TAOK2, VRK2, MAPK11, MAPK12, MAPK14 and MINK1 By similarity.
Sequence similarities	Belongs to the myelin basic protein family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Disease	Autoimmune encephalomyelitis
PTM	Acetylation Citrullination Methylation Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	myelin sheath Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	structural constituent of myelin sheath Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 167	167	Myelin basic protein		PRO_0000158988
Regions
	Region	45 – 87	43	Induces experimental autoimmune encephalomyelitis (EAE) 1
	Region	114 – 122	9	Induces experimental autoimmune encephalomyelitis (EAE) 2
Amino acid modifications
	Modified residue	1	1	N-acetylalanine
	Modified residue	7	1	Phosphoserine By similarity
	Modified residue	12	1	Phosphoserine By similarity
	Modified residue	25	1	Citrulline By similarity
	Modified residue	31	1	Citrulline By similarity
	Modified residue	35	1	Phosphothreonine By similarity
	Modified residue	40	1	Phosphoserine By similarity
	Modified residue	56	1	Phosphoserine By similarity
	Modified residue	69	1	Phosphotyrosine By similarity
	Modified residue	71	1	Phosphoserine By similarity
	Modified residue	94	1	Phosphothreonine By similarity
	Modified residue	97	1	Phosphothreonine By similarity
	Modified residue	102	1	Deamidated glutamine By similarity
	Modified residue	106	1	Symmetric dimethylarginine By similarity
	Modified residue	114	1	Phosphoserine By similarity
	Modified residue	129	1	Citrulline By similarity
	Modified residue	144	1	Deamidated glutamine By similarity
	Modified residue	156	1	Citrulline By similarity
	Modified residue	158	1	Phosphoserine By similarity
	Modified residue	162	1	Phosphoserine By similarity
	Modified residue	167	1	Citrulline By similarity

Sequences

	Sequence		Length	Mass (Da)
	P25188 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 866D31F1E5ACFEA6	FASTA	167	18,213
	10 20 30 40 50 60 ASQKRPSQRH GSKYLATAST MDHARHGFLP RHRDTGILDS IGRFFGSDRA APKRGSGKDS 70 80 90 100 110 120 HHAARTTHYG SLPQKSQRSQ DENPVVHFFK NIVTPRTPPP SQGKGRGLSL SRFSWGAEGQ 130 140 150 160 KPGFGYGGRA DYKSKGFKGA HDAQGTLSKI FKLGGRDSRS GSPMARR

References

[1]	"Sequence of guinea pig myelin basic protein." Deibler G.E., Martenson R.E., Krutzsch H.C., Kies M.W. J. Neurochem. 43:100-105(1984) [PubMed: 6202840] [Abstract] Cited for: PROTEIN SEQUENCE.
[2]	"DNA vaccination using Guinea pig myelin basic protein coding region in experimental autoimmune encephalomyelitis." Kim G., Tanuma N., Matsumoto Y. Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 7-156. Strain: Hartley. Tissue: Spinal cord.
[3]	"Encephalitogenic fragment of myelin basic protein. Amino acid sequence of bovine, rabbit, guinea pig, monkey, and human fragments." Shapira R., McKneally S.S., Chou F., Kibler R.F. J. Biol. Chem. 246:4630-4640(1971) Cited for: PROTEIN SEQUENCE OF 45-87.
[4]	"The contribution of phosphorylation and loss of COOH-terminal arginine to the microheterogeneity of myelin basic protein." Deibler G.E., Martenson R.E., Kramer A.J., Kies M.W. J. Biol. Chem. 250:7931-7938(1975) [PubMed: 51849] [Abstract] Cited for: POST-TRANSLATIONAL MODIFICATIONS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF074337 mRNA. Translation: AAC26130.1.
PIR	A37246.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P25188.
Proteomic databases
PRIDE	P25188.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSCPOT00000008505; ENSCPOP00000007579; ENSCPOG00000008430. ENSCPOT00000008506; ENSCPOP00000007580; ENSCPOG00000008430.
Phylogenomic databases
eggNOG	roNOG05523.
GeneTree	ENSGT00390000014772.
HOVERGEN	HBG008347.
InParanoid	P25188.
OrthoDB	EOG4S1T86.
Family and domain databases
InterPro	IPR000548. Myelin_BP. [Graphical view]
PANTHER	PTHR11429. Myelin_BP. 1 hit.
Pfam	PF01669. Myelin_MBP. 1 hit. [Graphical view]
PRINTS	PR00212. MYELINMBP.
PROSITE	PS00569. MYELIN_MBP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MBP_CAVPO

Accession

Primary (citable) accession number: P25188

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program