P25171 (RCC1_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Regulator of chromosome condensation

Alternative name(s):
Chromatin-binding protein Bj1

Gene names

Name:	Bj1
ORF Names:	CG10480

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	547 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds to the chromatin.
Subcellular location	Nucleus. Cytoplasm. Note: Becomes dispersed throughout the cytoplasm during mitosis. Is associated with chromatin throughout mitosis. Ref.5 Ref.6
Sequence similarities	Contains 7 RCC1 repeats.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Cytoplasm Nucleus
Domain	Repeat
Molecular function	Guanine-nucleotide releasing factor
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell division Inferred from electronic annotation. Source: UniProtKB-KW central nervous system development Inferred from direct assay Ref.1. Source: UniProtKB mitosis Inferred from electronic annotation. Source: UniProtKB-KW regulation of mitotic cell cycle Inferred from direct assay Ref.6. Source: UniProtKB regulation of neurogenesis Inferred from mutant phenotype. Source: FlyBase regulation of nucleocytoplasmic transport Inferred from mutant phenotype. Source: FlyBase
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay Ref.1. Source: UniProtKB
Molecular function	chromatin binding Inferred from direct assay Ref.6. Source: UniProtKB guanyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 547

547

Regulator of chromosome condensation

PRO_0000206633

Regions

Repeat

41 – 91

51

RCC1 1

Repeat

92 – 143

52

RCC1 2

Repeat

145 – 195

51

RCC1 3

Repeat

197 – 257

61

RCC1 4

Repeat

260 – 310

51

RCC1 5

Repeat

311 – 362

52

RCC1 6

Repeat

363 – 416

54

RCC1 7

Repeat

417 – 454

38

Bj1 1

Repeat

455 – 489

35

Bj1 2

Repeat

490 – 519

30

Bj1 3

Compositional bias

520 – 547

28

Glu/Lys-rich

Amino acid modifications

Modified residue

286

1

Phosphothreonine Ref.7

Modified residue

512

1

Phosphothreonine Ref.7

Modified residue

526

1

Phosphoserine Ref.7

Modified residue

531

1

Phosphothreonine Ref.7

Experimental info

Sequence conflict

490

1

V → A in CAA41417. Ref.1

Secondary structure

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547

Helix Strand Turn

Details...

Beta strand

44 – 47

4

Beta strand

75 – 80

6

Beta strand

86 – 90

5

Beta strand

95 – 98

4

Beta strand

128 – 132

5

Beta strand

138 – 142

5

Beta strand

147 – 150

4

Beta strand

172 – 174

3

Beta strand

180 – 184

5

Beta strand

190 – 194

5

Beta strand

199 – 202

4

Turn

215 – 217

3

Turn

227 – 229

3

Beta strand

233 – 235

3

Beta strand

243 – 249

7

Beta strand

252 – 257

6

Turn

258 – 260

3

Beta strand

263 – 266

4

Beta strand

288 – 290

3

Beta strand

294 – 299

6

Beta strand

305 – 309

5

Beta strand

314 – 317

4

Beta strand

347 – 349

3

Beta strand

357 – 361

5

Beta strand

366 – 369

4

Turn

394 – 396

3

Beta strand

401 – 405

5

Beta strand

411 – 415

5

Sequences

	Sequence		Length	Mass (Da)
	P25171 [UniParc]. Last modified June 1, 2001. Version 2. Checksum: 77D9744EBAF4597A	FASTA	547	58,851
	10 20 30 40 50 60 MPRRKALTNN NNAGEAEQQP PKAKRARIAF HLELPKRRTV LGNVLVCGNG DVGQLGLGED 70 80 90 100 110 120 ILERKRLSPV AGIPDAVDIS AGGMHNLVLT KSGDIYSFGC NDEGALGRDT SEDGSESKPD 130 140 150 160 170 180 LIDLPGKALC ISAGDSHSAC LLEDGRVFAW GSFRDSHGNM GLTIDGNKRT PIDLMEGTVC 190 200 210 220 230 240 CSIASGADHL VILTTAGKVF TVGCAEQGQL GRLSERSISG EGRRGKRDLL RPTQLIITRA 250 260 270 280 290 300 KPFEAIWATN YCTFMRESQT QVIWATGLNN FKQLAHETKG KEFALTPIKT ELKDIRHIAG 310 320 330 340 350 360 GQHHTVILTT DLKCSVVGRP EYGRLGLGDV KDVVEKPTIV KKLTEKIVSV GCGEVCSYAV 370 380 390 400 410 420 TIDGKLYSWG SGVNNQLGVG DGDDELEPIV VVSKNTQGKH MLLASGGGQH AIFLVKADKQ 430 440 450 460 470 480 DQKENVPVKV SGSSSISKKD KTPPQDNVDK EAENVDKQEQ KENLPAKAST SSKKNKTPPQ 490 500 510 520 530 540 DNADKEAENV DKQEQKENLP AKASTSSKKI KTPPQDDAAE EVEEESAQEP TPKKAKKPAA KRGGKKT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The maternally expressed Drosophila gene encoding the chromatin-binding protein BJ1 is a homolog of the vertebrate gene Regulator of Chromatin Condensation, RCC1." Frasch M. EMBO J. 10:1225-1236(1991) [PubMed: 2022188] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[5]	"Mutation of the hamster cell cycle gene RCC1 is complemented by the homologous genes of Drosophila and S.cerevisiae." Ohtsubo M., Yoshida T., Seino H., Nishitani H., Clark K.L., Sprague G.F. Jr., Frasch M., Nishimoto T. EMBO J. 10:1265-1273(1991) [PubMed: 2022190] [Abstract] Cited for: CHARACTERIZATION, SUBCELLULAR LOCATION.
[6]	"Ran localizes around the microtubule spindle in vivo during mitosis in Drosophila embryos." Trieselmann N., Wilde A. Curr. Biol. 12:1124-1129(2002) [PubMed: 12121620] [Abstract] Cited for: SUBCELLULAR LOCATION.
[7]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-286; THR-512; SER-526 AND THR-531, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X58530 Genomic DNA. Translation: CAA41417.1.
AE014296 Genomic DNA. Translation: AAF50726.1.
AE014296 Genomic DNA. Translation: AAF50727.1.
AE014296 Genomic DNA. Translation: AAN12235.1.
AY070540 mRNA. Translation: AAL48011.1.

PIR

S15028.

RefSeq

NP_523943.1. NM_079219.3.
NP_729118.1. NM_168151.2.
NP_729119.1. NM_168152.2.

UniGene

Dm.8023.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3MVD	X-ray	2.90	K/L	2-422	[»]

ProteinModelPortal

P25171.

SMR

P25171. Positions 28-421.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-17766N.

IntAct

P25171. 4 interactions.

MINT

MINT-781282.

STRING

P25171.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblMetazoa

FBtr0077074; FBpp0076782; FBgn0002638.
FBtr0077075; FBpp0076783; FBgn0002638.
FBtr0077076; FBpp0076784; FBgn0002638.

GeneID

38669.

KEGG

dme:Dmel_CG10480.

Organism-specific databases

CTD

38669.

FlyBase

FBgn0002638. Bj1.

Phylogenomic databases

eggNOG

inNOG04463.

GeneTree

EMGT00050000004665.

InParanoid

P25171.

OMA

KNKEVKR.

OrthoDB

EOG437PW4.

PhylomeDB

P25171.

Gene expression databases

ArrayExpress

P25171.

Bgee

P25171.

GermOnline

CG10480. Drosophila melanogaster.

Family and domain databases

InterPro

IPR000408. Reg_chr_condens.
IPR009091. Reg_csome_cond/b-lactamase_inh.
[Graphical view]

Gene3D

G3DSA:2.130.10.30. Reg_csome_cond/b-lactamase_inh. 1 hit.

KO

K11493.

Pfam

PF00415. RCC1. 4 hits.
[Graphical view]

PRINTS

PR00633. RCCNDNSATION.

SUPFAM

SSF50985. RCC1/BLIP-II. 1 hit.

PROSITE

PS00625. RCC1_1. 1 hit.
PS00626. RCC1_2. 2 hits.
PS50012. RCC1_3. 6 hits.
[Graphical view]

ProtoNet

Search...

Other

NextBio

809817.

Entry information

Entry name

RCC1_DROME

Accession

Primary (citable) accession number: P25171
Secondary accession number(s): A4V1J1, Q9VRR5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 109 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project