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P25170 (TRPG_PHACH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Multifunctional tryptophan biosynthesis protein

Including the following 3 domains:

  1. Anthranilate synthase component 2
    Short name=AS
    EC=4.1.3.27
    Alternative name(s):
    Anthranilate synthase, glutamine amidotransferase component
  2. Indole-3-glycerol phosphate synthase
    Short name=IGPS
    EC=4.1.1.48
  3. N-(5'-phosphoribosyl)anthranilate isomerase
    Short name=PRAI
    EC=5.3.1.24
Gene names
Name:TRPC
OrganismPhanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
Taxonomic identifier5306 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesCorticialesCorticiaceaePhanerochaete

Protein attributes

Sequence length788 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Trifunctional enzyme bearing the Gln amidotransferase (GATase) domain of anthranilate synthase, indole-glycerolphosphate synthase, and phosphoribosylanthranilate isomerase activities. HAMAP-Rule MF_00134_B

Catalytic activity

N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate. HAMAP-Rule MF_00134_B

1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO2 + H2O. HAMAP-Rule MF_00134_B

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate. HAMAP-Rule MF_00134_B

Pathway

Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5. HAMAP-Rule MF_00134_B

Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 3/5.

Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 4/5.

Sequence similarities

Contains 1 glutamine amidotransferase type-1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 788788Multifunctional tryptophan biosynthesis protein HAMAP-Rule MF_00134_B
PRO_0000056863

Regions

Domain12 – 207196Glutamine amidotransferase type-1
Region238 – 503266Indole-3-glycerol phosphate synthase HAMAP-Rule MF_00134_B
Region520 – 788269N-(5'-phosphoribosyl)anthranilate isomerase HAMAP-Rule MF_00134_B

Sites

Active site911For GATase activity By similarity
Active site1811For GATase activity By similarity
Active site1831For GATase activity By similarity

Sequences

Sequence LengthMass (Da)Tools
P25170 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: 20108D088803D787

FASTA78885,345
        10         20         30         40         50         60 
MSLPPHLCVP IDILMIDNFD SFTWNLYQSL CLLGADVTVI RNDAIPRSAI PQLRIKRLIV 

        70         80         90        100        110        120 
SPGPGHPQTD SGISREAIKY FAGKVPIMGV CMGLECVVDV FGGQIAYAGE IMHGKVSGIR 

       130        140        150        160        170        180 
HDARGCFKDL PQGIQSTRYH SLSAGVKTLP DELAVTAVTE HERVIMGIRH RKYTVEAVQY 

       190        200        210        220        230        240 
HPESILSESG DDLLRHFMKL KGGTWEENPE FRVLDPSLPA FEIGSQPAAS TSAKIPTILE 

       250        260        270        280        290        300 
KICAQRQKDV DQAKATPGTT PEDLKTLLSM KLSPPQISFP DRLKAAEVKP ALMAEVKRAS 

       310        320        330        340        350        360 
PSKGPIAMNG NAAQQALTYA LAGASVISVL TEPTWFKGSL LDMRLARQAI DNLPHRPAIL 

       370        380        390        400        410        420 
RKDFIIDEYQ IDEARLHGAD TVLLIVATLT EKRLEELYAY SQSLGMEPLV EVNNAKEMEI 

       430        440        450        460        470        480 
ALKLGAKVIG VNNRNLHDFN VDMGTTSRLA EMVRERDVIL CALSGIKDAQ DVNTYVEQGV 

       490        500        510        520        530        540 
GAVLVGESLM RAPDTRAFIR QLLSIPESEA KGKGKETTPL SRSCGIRTEE EALAAAEAGA 

       550        560        570        580        590        600 
DMLGLMFVPK SKRYVSLEKA QQIAAAIHSR RLSKPVLTSG KTLENEPWFT AQQPPSRSFV 

       610        620        630        640        650        660 
WRFAARPLLV GVFQNQPLSV YLTPCVPQLD LVQLHGSEPA ELAKHYPVPV IRVFHVSADG 

       670        680        690        700        710        720 
RGLADLARPG LHQFALLDAV LPGSASALSG GTGTTVDWAL ARDAVRSGEV RVARSAQGDG 

       730        740        750        760        770        780 
PVLSLNSSEA LYPMPVILAG GPQPENVSEA VDTVAPWAVD VSGGVELEDG SGKDLEKVRA 


FVKAAKKL 

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References

[1]"The trpC gene of Phanerochaete chrysosporium is unique in containing an intron but nevertheless maintains the order of functional domains seen in other fungi."
Schrank A., Tempelaars C., Sims P.F.G., Oliver S.G., Broda P.
Mol. Microbiol. 5:467-476(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 34541 / NBRC 31249 / ME-446 / PRL 2750.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X56047 Genomic DNA. Translation: CAA39518.1.
PIRS15239.

3D structure databases

ProteinModelPortalP25170.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5306.JGI27842.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGCOG0134.

Enzyme and pathway databases

UniPathwayUPA00035; UER00040.
UPA00035; UER00042.
UPA00035; UER00043.

Family and domain databases

Gene3D3.20.20.70. 3 hits.
3.40.50.880. 1 hit.
HAMAPMF_00134_B. IGPS_B.
MF_00135. PRAI.
InterProIPR013785. Aldolase_TIM.
IPR016302. Anthranilate_synth_II.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR013798. Indole-3-glycerol_P_synth.
IPR001468. Indole-3-GlycerolPSynthase_CS.
IPR001240. PRAI_dom.
IPR011060. RibuloseP-bd_barrel.
IPR006221. TrpG/PapA_dom.
[Graphical view]
PfamPF00117. GATase. 1 hit.
PF00218. IGPS. 1 hit.
PF00697. PRAI. 1 hit.
[Graphical view]
PIRSFPIRSF001382. TrpG-trpC-trpF. 1 hit.
SUPFAMSSF51366. SSF51366. 3 hits.
SSF52317. SSF52317. 1 hit.
TIGRFAMsTIGR00566. trpG_papA. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
PS00614. IGPS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRPG_PHACH
AccessionPrimary (citable) accession number: P25170
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: June 11, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways