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Protein

Multifunctional tryptophan biosynthesis protein

Gene

TRPC

Organism
Phanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Trifunctional enzyme bearing the Gln amidotransferase (GATase) domain of anthranilate synthase, indole-glycerolphosphate synthase, and phosphoribosylanthranilate isomerase activities.

Catalytic activityi

N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate.
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO2 + H2O.
Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 1, 3 and 4 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Multifunctional tryptophan biosynthesis protein (TRPC)
  2. no protein annotated in this organism
  3. Multifunctional tryptophan biosynthesis protein (TRPC)
  4. Multifunctional tryptophan biosynthesis protein (TRPC)
  5. no protein annotated in this organism
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei91Nucleophile; for GATase activityBy similarity1
Active sitei181For GATase activityBy similarity1
Active sitei183For GATase activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylase, Isomerase, Lyase, Multifunctional enzyme
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040
UPA00035; UER00042
UPA00035; UER00043

Names & Taxonomyi

Protein namesi
Recommended name:
Multifunctional tryptophan biosynthesis protein
Including the following 3 domains:
Anthranilate synthase component 2 (EC:4.1.3.27)
Short name:
AS
Alternative name(s):
Anthranilate synthase, glutamine amidotransferase component
Indole-3-glycerol phosphate synthase (EC:4.1.1.48)
Short name:
IGPS
N-(5'-phosphoribosyl)anthranilate isomerase (EC:5.3.1.24)
Short name:
PRAI
Gene namesi
Name:TRPC
OrganismiPhanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
Taxonomic identifieri5306 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesPolyporalesPhanerochaetaceaePhanerochaete

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000568631 – 788Multifunctional tryptophan biosynthesis proteinAdd BLAST788

Proteomic databases

PRIDEiP25170

Structurei

3D structure databases

ProteinModelPortaliP25170
SMRiP25170
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 207Glutamine amidotransferase type-1Add BLAST196

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni63 – 65Glutamine bindingBy similarity3
Regioni141 – 142Glutamine bindingBy similarity2
Regioni238 – 503Indole-3-glycerol phosphate synthaseAdd BLAST266
Regioni520 – 788N-(5'-phosphoribosyl)anthranilate isomeraseAdd BLAST269

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiKOG0026 Eukaryota
KOG4201 Eukaryota
KOG4202 Eukaryota
COG0134 LUCA
COG0135 LUCA
COG0512 LUCA

Family and domain databases

CDDicd00331 IGPS, 1 hit
cd00405 PRAI, 1 hit
Gene3Di3.20.20.70, 3 hits
3.40.50.880, 1 hit
HAMAPiMF_00134_B IGPS_B, 1 hit
MF_00135 PRAI, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR016302 Anthranilate_synth_II
IPR029062 Class_I_gatase-like
IPR017926 GATASE
IPR013798 Indole-3-glycerol_P_synth
IPR001468 Indole-3-GlycerolPSynthase_CS
IPR001240 PRAI
IPR011060 RibuloseP-bd_barrel
IPR006221 TrpG/PapA_dom
PfamiView protein in Pfam
PF00117 GATase, 1 hit
PF00218 IGPS, 1 hit
PF00697 PRAI, 1 hit
PIRSFiPIRSF001382 TrpG-trpC-trpF, 1 hit
SUPFAMiSSF51366 SSF51366, 3 hits
SSF52317 SSF52317, 1 hit
TIGRFAMsiTIGR00566 trpG_papA, 1 hit
PROSITEiView protein in PROSITE
PS51273 GATASE_TYPE_1, 1 hit
PS00614 IGPS, 1 hit

Sequencei

Sequence statusi: Complete.

P25170-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLPPHLCVP IDILMIDNFD SFTWNLYQSL CLLGADVTVI RNDAIPRSAI
60 70 80 90 100
PQLRIKRLIV SPGPGHPQTD SGISREAIKY FAGKVPIMGV CMGLECVVDV
110 120 130 140 150
FGGQIAYAGE IMHGKVSGIR HDARGCFKDL PQGIQSTRYH SLSAGVKTLP
160 170 180 190 200
DELAVTAVTE HERVIMGIRH RKYTVEAVQY HPESILSESG DDLLRHFMKL
210 220 230 240 250
KGGTWEENPE FRVLDPSLPA FEIGSQPAAS TSAKIPTILE KICAQRQKDV
260 270 280 290 300
DQAKATPGTT PEDLKTLLSM KLSPPQISFP DRLKAAEVKP ALMAEVKRAS
310 320 330 340 350
PSKGPIAMNG NAAQQALTYA LAGASVISVL TEPTWFKGSL LDMRLARQAI
360 370 380 390 400
DNLPHRPAIL RKDFIIDEYQ IDEARLHGAD TVLLIVATLT EKRLEELYAY
410 420 430 440 450
SQSLGMEPLV EVNNAKEMEI ALKLGAKVIG VNNRNLHDFN VDMGTTSRLA
460 470 480 490 500
EMVRERDVIL CALSGIKDAQ DVNTYVEQGV GAVLVGESLM RAPDTRAFIR
510 520 530 540 550
QLLSIPESEA KGKGKETTPL SRSCGIRTEE EALAAAEAGA DMLGLMFVPK
560 570 580 590 600
SKRYVSLEKA QQIAAAIHSR RLSKPVLTSG KTLENEPWFT AQQPPSRSFV
610 620 630 640 650
WRFAARPLLV GVFQNQPLSV YLTPCVPQLD LVQLHGSEPA ELAKHYPVPV
660 670 680 690 700
IRVFHVSADG RGLADLARPG LHQFALLDAV LPGSASALSG GTGTTVDWAL
710 720 730 740 750
ARDAVRSGEV RVARSAQGDG PVLSLNSSEA LYPMPVILAG GPQPENVSEA
760 770 780
VDTVAPWAVD VSGGVELEDG SGKDLEKVRA FVKAAKKL
Length:788
Mass (Da):85,345
Last modified:May 1, 1992 - v1
Checksum:i20108D088803D787
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56047 Genomic DNA Translation: CAA39518.1
PIRiS15239

Similar proteinsi

Entry informationi

Entry nameiTRPG_PHACH
AccessioniPrimary (citable) accession number: P25170
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: April 25, 2018
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health