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Protein

Multifunctional tryptophan biosynthesis protein

Gene

TRPC

Organism
Phanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Trifunctional enzyme bearing the Gln amidotransferase (GATase) domain of anthranilate synthase, indole-glycerolphosphate synthase, and phosphoribosylanthranilate isomerase activities.

Catalytic activityi

N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate.
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO2 + H2O.
Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

Pathway: L-tryptophan biosynthesis

This protein is involved in step 1, 3 and 4 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Multifunctional tryptophan biosynthesis protein (TRPC)
  2. no protein annotated in this organism
  3. Multifunctional tryptophan biosynthesis protein (TRPC)
  4. Multifunctional tryptophan biosynthesis protein (TRPC)
  5. no protein annotated in this organism
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei91 – 911For GATase activityBy similarity
Active sitei181 – 1811For GATase activityBy similarity
Active sitei183 – 1831For GATase activityBy similarity

GO - Molecular functioni

GO - Biological processi

Keywords - Molecular functioni

Decarboxylase, Isomerase, Lyase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040.
UPA00035; UER00042.
UPA00035; UER00043.

Names & Taxonomyi

Protein namesi
Recommended name:
Multifunctional tryptophan biosynthesis protein
Including the following 3 domains:
Anthranilate synthase component 2 (EC:4.1.3.27)
Short name:
AS
Alternative name(s):
Anthranilate synthase, glutamine amidotransferase component
Indole-3-glycerol phosphate synthase (EC:4.1.1.48)
Short name:
IGPS
N-(5'-phosphoribosyl)anthranilate isomerase (EC:5.3.1.24)
Short name:
PRAI
Gene namesi
Name:TRPC
OrganismiPhanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
Taxonomic identifieri5306 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesPolyporalesPhanerochaetaceaePhanerochaete

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 788788Multifunctional tryptophan biosynthesis proteinPRO_0000056863Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi5306.JGI27842.

Structurei

3D structure databases

ProteinModelPortaliP25170.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 207196Glutamine amidotransferase type-1Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni238 – 503266Indole-3-glycerol phosphate synthaseAdd
BLAST
Regioni520 – 788269N-(5'-phosphoribosyl)anthranilate isomeraseAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0134.

Family and domain databases

Gene3Di3.20.20.70. 3 hits.
3.40.50.880. 1 hit.
HAMAPiMF_00134_B. IGPS_B.
MF_00135. PRAI.
InterProiIPR013785. Aldolase_TIM.
IPR016302. Anthranilate_synth_II.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR013798. Indole-3-glycerol_P_synth.
IPR001468. Indole-3-GlycerolPSynthase_CS.
IPR001240. PRAI.
IPR011060. RibuloseP-bd_barrel.
IPR006221. TrpG/PapA_dom.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00218. IGPS. 1 hit.
PF00697. PRAI. 1 hit.
[Graphical view]
PIRSFiPIRSF001382. TrpG-trpC-trpF. 1 hit.
SUPFAMiSSF51366. SSF51366. 3 hits.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00566. trpG_papA. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS00614. IGPS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25170-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLPPHLCVP IDILMIDNFD SFTWNLYQSL CLLGADVTVI RNDAIPRSAI
60 70 80 90 100
PQLRIKRLIV SPGPGHPQTD SGISREAIKY FAGKVPIMGV CMGLECVVDV
110 120 130 140 150
FGGQIAYAGE IMHGKVSGIR HDARGCFKDL PQGIQSTRYH SLSAGVKTLP
160 170 180 190 200
DELAVTAVTE HERVIMGIRH RKYTVEAVQY HPESILSESG DDLLRHFMKL
210 220 230 240 250
KGGTWEENPE FRVLDPSLPA FEIGSQPAAS TSAKIPTILE KICAQRQKDV
260 270 280 290 300
DQAKATPGTT PEDLKTLLSM KLSPPQISFP DRLKAAEVKP ALMAEVKRAS
310 320 330 340 350
PSKGPIAMNG NAAQQALTYA LAGASVISVL TEPTWFKGSL LDMRLARQAI
360 370 380 390 400
DNLPHRPAIL RKDFIIDEYQ IDEARLHGAD TVLLIVATLT EKRLEELYAY
410 420 430 440 450
SQSLGMEPLV EVNNAKEMEI ALKLGAKVIG VNNRNLHDFN VDMGTTSRLA
460 470 480 490 500
EMVRERDVIL CALSGIKDAQ DVNTYVEQGV GAVLVGESLM RAPDTRAFIR
510 520 530 540 550
QLLSIPESEA KGKGKETTPL SRSCGIRTEE EALAAAEAGA DMLGLMFVPK
560 570 580 590 600
SKRYVSLEKA QQIAAAIHSR RLSKPVLTSG KTLENEPWFT AQQPPSRSFV
610 620 630 640 650
WRFAARPLLV GVFQNQPLSV YLTPCVPQLD LVQLHGSEPA ELAKHYPVPV
660 670 680 690 700
IRVFHVSADG RGLADLARPG LHQFALLDAV LPGSASALSG GTGTTVDWAL
710 720 730 740 750
ARDAVRSGEV RVARSAQGDG PVLSLNSSEA LYPMPVILAG GPQPENVSEA
760 770 780
VDTVAPWAVD VSGGVELEDG SGKDLEKVRA FVKAAKKL
Length:788
Mass (Da):85,345
Last modified:May 1, 1992 - v1
Checksum:i20108D088803D787
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56047 Genomic DNA. Translation: CAA39518.1.
PIRiS15239.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56047 Genomic DNA. Translation: CAA39518.1.
PIRiS15239.

3D structure databases

ProteinModelPortaliP25170.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5306.JGI27842.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiCOG0134.

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040.
UPA00035; UER00042.
UPA00035; UER00043.

Family and domain databases

Gene3Di3.20.20.70. 3 hits.
3.40.50.880. 1 hit.
HAMAPiMF_00134_B. IGPS_B.
MF_00135. PRAI.
InterProiIPR013785. Aldolase_TIM.
IPR016302. Anthranilate_synth_II.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR013798. Indole-3-glycerol_P_synth.
IPR001468. Indole-3-GlycerolPSynthase_CS.
IPR001240. PRAI.
IPR011060. RibuloseP-bd_barrel.
IPR006221. TrpG/PapA_dom.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00218. IGPS. 1 hit.
PF00697. PRAI. 1 hit.
[Graphical view]
PIRSFiPIRSF001382. TrpG-trpC-trpF. 1 hit.
SUPFAMiSSF51366. SSF51366. 3 hits.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00566. trpG_papA. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS00614. IGPS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The trpC gene of Phanerochaete chrysosporium is unique in containing an intron but nevertheless maintains the order of functional domains seen in other fungi."
    Schrank A., Tempelaars C., Sims P.F.G., Oliver S.G., Broda P.
    Mol. Microbiol. 5:467-476(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 34541 / NBRC 31249 / ME-446 / PRL 2750.

Entry informationi

Entry nameiTRPG_PHACH
AccessioniPrimary (citable) accession number: P25170
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: April 1, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.