P25169 (AT1B1_ARTSF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Sodium/potassium-transporting ATPase subunit beta Alternative name(s): Sodium/potassium-dependent ATPase beta subunit
Organism	Artemia franciscana (Brine shrimp) (Artemia sanfranciscana)
Taxonomic identifier	6661 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Crustacea › Branchiopoda › Anostraca › Artemiidae › Artemia

Protein attributes

Sequence length	315 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.
Subunit structure	Composed of three subunits: alpha (catalytic), beta and gamma.
Subcellular location	Membrane; Single-pass type II membrane protein.
Sequence similarities	Belongs to the X(+)/potassium ATPases subunit beta family.

Ontologies

Keywords
Biological process	Ion transport Potassium transport Sodium transport Sodium/potassium transport Transport
Cellular component	Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Potassium Sodium
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	sodium:potassium-exchanging ATPase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 315	315	Sodium/potassium-transporting ATPase subunit beta		PRO_0000219113
Regions
	Topological domain	1 – 47	47	Cytoplasmic Potential
	Transmembrane	48 – 68	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	69 – 315	247	Extracellular Potential
Amino acid modifications
	Glycosylation	198	1	N-linked (GlcNAc...) Potential
	Glycosylation	222	1	N-linked (GlcNAc...) Potential
	Glycosylation	296	1	N-linked (GlcNAc...) Potential
	Disulfide bond	145 ↔ 157		By similarity
	Disulfide bond	168 ↔ 182		By similarity
	Disulfide bond	233 ↔ 290		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P25169 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 787BAA38F99566E8	FASTA	315	35,955
	10 20 30 40 50 60 MADKKPDEQF VGSGPKETKW QSFKGFVWNS ETSQFMGRTA GSWAKITIFY VIFYTLLAGF 70 80 90 100 110 120 FAGMLMIFYQ TLDFKIPKWQ NKDSLIGANP GLGFRPMPPE AQVDSTLIQF KHGIKGDWQY 130 140 150 160 170 180 WVHSLTEFLE PYETLTSSGQ EFTNCDFDKP PQEGKACNFN VELLGDHCTK ENNFGYELGK 190 200 210 220 230 240 PCVLIKLTDF GWRPEVYNSS AEVPEDMPAD LKSYIKDIET GNKTHMNMVW LSCEGETAND 250 260 270 280 290 300 KEKIGTITYT PFRGFPAYYY PYLNVPGYLT PVVALQFGSL QNGQAVNVEC KAWANNISRD 310 RQRRLGSVHF EIRMD

References

[1]

"Molecular cloning of the beta-subunit of the Na,K-ATPase in the brine shrimp, Artemia. The cDNA-derived amino acid sequence shows low homology with the beta-subunits of vertebrates except in the single transmembrane and the carboxy-terminal domains." Bhattacharyya K.K., Bergstroem E.E., Hokin L.E. FEBS Lett. 269:233-238(1990) [PubMed: 2167242] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X55780 mRNA. Translation: CAA39301.1.
3D structure databases
ProteinModelPortal	P25169.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000402. ATPase_P-typ_cation-exchng_bsu. [Graphical view]
PANTHER	PTHR11523. ATPase_H_Na/K_b. 1 hit.
Pfam	PF00287. Na_K-ATPase. 1 hit. [Graphical view]
PROSITE	PS00390. ATPASE_NA_K_BETA_1. 1 hit. PS00391. ATPASE_NA_K_BETA_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AT1B1_ARTSF

Accession

Primary (citable) accession number: P25169

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	September 21, 2011
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)