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P25167 (RPC2_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-directed RNA polymerase III subunit RPC2
Short name=RNA polymerase III subunit C2
EC=2.7.7.6
Alternative name(s):
DNA-directed RNA polymerase III 128 kDa polypeptide
Short name=C128
Gene names
Name:RpIII128
Synonyms:RP128
ORF Names:CG8344
OrganismDrosophila melanogaster (Fruit fly)
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length1137 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft By similarity.

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Subunit structure

Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the RNA polymerase beta chain family.

Sequence caution

The sequence AAM51972.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processTranscription
   Cellular componentDNA-directed RNA polymerase
Nucleus
   DomainZinc-finger
   LigandMetal-binding
Zinc
   Molecular functionNucleotidyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionDNA binding

Inferred from electronic annotation. Source: InterPro

DNA-directed RNA polymerase activity

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

ribonucleoside binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11371137DNA-directed RNA polymerase III subunit RPC2
PRO_0000048094

Regions

Zinc finger1084 – 109916C4-type

Sites

Metal binding10861Zinc By similarity
Metal binding10891Zinc By similarity
Metal binding10981Zinc By similarity
Metal binding11011Zinc By similarity

Experimental info

Sequence conflict1501Missing in CAA41631. Ref.1
Sequence conflict4901D → Y in CAA41631. Ref.1
Sequence conflict6681P → T in CAA41631. Ref.1
Sequence conflict708 – 7092QK → HN in CAA41631. Ref.1
Sequence conflict898 – 8992EI → R in CAA41631. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P25167 [UniParc].

Last modified October 25, 2005. Version 2.
Checksum: 043EC430D998E1FD

FASTA1,137128,515
        10         20         30         40         50         60 
MVELKMGDHN VEATTWDPGD SKDWSVPIKP LTEKWKLVPA FLQVKGLVKQ HIDSFNHFIN 

        70         80         90        100        110        120 
VDIKKIVKAN ELVTSGADPL FYLKYLDVRV GKPDIDDGFN ITKATTPHEC RLRDTTYSAP 

       130        140        150        160        170        180 
ITVDIEYTRG TQRIKRNNLL IGRMPLMLRC SNCALTGKSE FELSKLNECP LDPGGYFVVR 

       190        200        210        220        230        240 
GQEKVILIQE QLSWNKMLTE DFNGVVQCQV TSSTHEKKSR TLVLSKHGKY YLKHNSMTDD 

       250        260        270        280        290        300 
IPIVVIFKAL GVVSDQEIQS LIGIDSKSQN RFGASLIDAY NLKVFTQQRA LEYMGSKLVV 

       310        320        330        340        350        360 
KRFQSATTKT PSEEARELLL TTILAHVPVD NFNLQMKAIY VSMMVRRVMA AELDKTLFDD 

       370        380        390        400        410        420 
RDYYGNKRLE LAGSLLSMMF EDLFKRMNWE LKTIADKNIP KVKAAQFDVV KHMRAAQITA 

       430        440        450        460        470        480 
GLESAISSGN WTIKRFKMER AGVTQVLSRL SYISALGMMT RVNSQFEKTR KVSGPRSLQP 

       490        500        510        520        530        540 
SQWGMLCPSD TPEGEACGLV KNLALMTHIT TEVEERPVMI VAFNAGVEDI REVSGNPINN 

       550        560        570        580        590        600 
PNVFLVFING NVLGLTLNHK HLVRNLRYMR RKGRMGSYVS VHTSYTQRCI YIHTDGGRLC 

       610        620        630        640        650        660 
RPYVIVENRR PLVKQHHLDE LNRGIRKFDD FLLDGLIEYL DVNEENDSFI AWNEDQIEDR 

       670        680        690        700        710        720 
TTHLEIEPFT LLGVCAGLVP YPHHNQSPRN TYQCAMGKQA MGMIGYNQKN RIDSLMYNLV 

       730        740        750        760        770        780 
YPHAPMVKSK TIELTNFDKL PAGQNATVAV MSYSGYDIED ALILNKASID RGYGRCLVYK 

       790        800        810        820        830        840 
NSKCTVKRYA NQTFDRIMGP MKDALTNKVI FKHDVLDTDG IVAPGEQVQN KQIMINKEMP 

       850        860        870        880        890        900 
AVTSMNPLQG QSAQVPYTAV PISYKGPEPS YIERVMVSAN AEEDFLIKIL LRQTRIPEIG 

       910        920        930        940        950        960 
DKFSSRHGQK GVTGLIVEQE DMPFNDFGIC PDMIMNPHGF PSRMTVGKTL ELLGGKAGLL 

       970        980        990       1000       1010       1020 
EGKFHYGTAF GGSKVEDIQA ELERHGFNYV GKDFFYSGIT GTPLEAYIYS GPVYYQKLKH 

      1030       1040       1050       1060       1070       1080 
MVQDKMHARA RGPKAVLTRQ PTQGRSREGG LRLGEMERDC LISYGASMLI MERLMISSDA 

      1090       1100       1110       1120       1130 
FEVDVCRTCG RMAYCSWCHF CQSSANVSKI SMPYACKLLF QELTSMNVVP KMILENY

« Hide

References

« Hide 'large scale' references
[1]"Identification of the genes coding for the second-largest subunits of RNA polymerases I and III of Drosophila melanogaster."
Seifarth W., Petersen G., Kontermann R., Riva M., Huet J.-C., Bautz E.K.F.
Mol. Gen. Genet. 228:424-432(1991) [PubMed: 1910149] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Oregon-R.
[2]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[3]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: Berkeley.
[4]"A Drosophila full-length cDNA resource."
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Berkeley.
Tissue: Embryo.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X58826 Genomic DNA. Translation: CAA41631.1.
AE013599 Genomic DNA. Translation: AAF58590.1.
AY121645 mRNA. Translation: AAM51972.1. Different initiation.
PIRRNFF32. S16894.
RefSeqNP_523706.1. NM_078982.2.
UniGeneDm.2692.

3D structure databases

ProteinModelPortalP25167.
SMRP25167. Positions 662-702.
ModBaseSearch...

Protein-protein interaction databases

IntActP25167. 2 interactions.
MINTMINT-285299.
STRINGP25167.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0087977; FBpp0087085; FBgn0004463.
GeneID36289.
KEGGdme:Dmel_CG8344.
NMPDRfig|7227.3.peg.4743.

Organism-specific databases

CTD36289.
FlyBaseFBgn0004463. RpIII128.

Phylogenomic databases

eggNOGinNOG07758.
GeneTreeEMGT00050000009230.
InParanoidP25167.
OMAFKMERAG.
OrthoDBEOG4C2FRC.
PhylomeDBP25167.

Gene expression databases

BgeeP25167.
GermOnlineCG8344. Drosophila melanogaster.

Family and domain databases

InterProIPR015712. DNA-dir_RNA_pol_su2.
IPR007120. DNA-dir_RNA_pol_su2_6.
IPR007121. RNA_pol_bsu_CS.
IPR007644. RNA_pol_bsu_protrusion.
IPR007642. RNA_pol_Rpb2_2.
IPR007645. RNA_pol_Rpb2_3.
IPR007646. RNA_pol_Rpb2_4.
IPR007647. RNA_pol_Rpb2_5.
IPR007641. RNA_pol_Rpb2_7.
IPR014724. RNA_pol_RPB2_OB-fold.
[Graphical view]
Gene3DG3DSA:2.40.270.10. G3DSA:2.40.270.10. 2 hits.
G3DSA:2.40.50.150. Ribosomal_L2. 1 hit.
KOK03021.
PANTHERPTHR20856. RNA_pol_I_sub2. 1 hit.
PfamPF04563. RNA_pol_Rpb2_1. 1 hit.
PF04561. RNA_pol_Rpb2_2. 1 hit.
PF04565. RNA_pol_Rpb2_3. 1 hit.
PF04566. RNA_pol_Rpb2_4. 1 hit.
PF04567. RNA_pol_Rpb2_5. 1 hit.
PF00562. RNA_pol_Rpb2_6. 1 hit.
PF04560. RNA_pol_Rpb2_7. 1 hit.
[Graphical view]
PROSITEPS01166. RNA_POL_BETA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio797763.

Entry information

Entry nameRPC2_DROME
AccessionPrimary (citable) accession number: P25167
Secondary accession number(s): Q8MRD5, Q9V649
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: October 25, 2005
Last modified: January 25, 2012
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents