Reviewed, UniProtKB/Swiss-Prot P25154 (ACPH_RABIT)

Names and origin

Protein names

Recommended name:
    Acylamino-acid-releasing enzyme
      Short name=AARE
    EC=3.4.19.1
Alternative name(s):
    Acyl-peptide hydrolase
      Short name=APH
    Acylaminoacyl-peptidase

Gene names

Name:

APEH

Organism

Oryctolagus cuniculus (Rabbit)

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	6 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This enzyme catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid and a peptide with a free N-terminus. It preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser.
Catalytic activity	Cleavage of an N-acetyl or N-formyl amino acid from the N-terminus of a polypeptide.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the peptidase S9C family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›6	›6	Acylamino-acid-releasing enzyme		PRO_0000122433
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine Ref.1
Experimental info
	Non-terminal residue	6	1

Sequences

	Sequence		Length	Mass (Da)
	P25154-1 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 6732D6C40B16F000	FASTA	6	775
	MERQVL

References

[1]	"N-terminal sequence analysis of N alpha-acetylated proteins after unblocking with N-acylaminoacyl-peptide hydrolase." Krishna R.G., Chin C.C.Q., Wold F. Anal. Biochem. 199:45-50(1991) [PubMed: 1807161] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Muscle.

Cross-references

Sequence databases
PIR	A49792.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	P25154.
Enzyme and pathway databases
BRENDA	3.4.19.1. 255.
Family and domain databases
InterPro	IPR002471. Pept_S9_AS. [Graphical view]
PROSITE	PS00708. PRO_ENDOPEP_SER. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACPH_RABIT

Accession

Primary (citable) accession number: P25154

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	June 16, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)