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Protein

Ubiquitin-conjugating enzyme E2-17 kDa

Gene

Ubc6

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins. Required for postreplication repair of UV-damaged DNA (PubMed:1902572). Involved in the negative regulation of the Ras/MAPK signaling pathway in the wing by acting with the putative E3 ligases poe, Kcmf1 and Ufd4 to mediate the ubiquitination and proteasomal degradation of rl/MAPK (PubMed:27552662).2 Publications

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei88Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • p53 binding Source: BHF-UCL
  • protein domain specific binding Source: BHF-UCL
  • ubiquitin conjugating enzyme activity Source: BHF-UCL
  • ubiquitin protein ligase activity Source: GO_Central
  • ubiquitin protein ligase binding Source: GO_Central
  • ubiquitin-protein transferase activity Source: FlyBase

GO - Biological processi

  • autophagy of mitochondrion Source: FlyBase
  • centrosome cycle Source: FlyBase
  • centrosome separation Source: FlyBase
  • DNA repair Source: FlyBase
  • endocytic recycling Source: FlyBase
  • histone ubiquitination Source: GO_Central
  • negative regulation of apoptotic process Source: FlyBase
  • negative regulation of DNA damage response, signal transduction by p53 class mediator Source: BHF-UCL
  • negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: BHF-UCL
  • positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: BHF-UCL
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: FlyBase
  • protein polyubiquitination Source: GO_Central
  • ubiquitin-dependent protein catabolic process Source: BHF-UCL

Keywordsi

Molecular functionTransferase
Biological processDNA damage, DNA repair, Ubl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-DME-8866654 E3 ubiquitin ligases ubiquitinate target proteins
R-DME-983168 Antigen processing: Ubiquitination & Proteasome degradation
SignaLinkiP25153
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2-17 kDa (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme 6
Ubiquitin carrier protein
Ubiquitin-protein ligase
Gene namesi
Name:Ubc6Imported
Synonyms:Dhr6, UbcD6Imported
ORF Names:CG2013Imported
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0004436 Ubc6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000825231 – 151Ubiquitin-conjugating enzyme E2-17 kDaAdd BLAST151

Proteomic databases

PaxDbiP25153
PRIDEiP25153

Expressioni

Gene expression databases

BgeeiFBgn0004436
ExpressionAtlasiP25153 baseline and differential
GenevisibleiP25153 DM

Interactioni

GO - Molecular functioni

  • p53 binding Source: BHF-UCL
  • protein domain specific binding Source: BHF-UCL
  • ubiquitin protein ligase binding Source: GO_Central

Protein-protein interaction databases

BioGridi65824, 1 interactor
STRINGi7227.FBpp0078490

Structurei

3D structure databases

ProteinModelPortaliP25153
SMRiP25153
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0419 Eukaryota
COG5078 LUCA
GeneTreeiENSGT00900000140930
InParanoidiP25153
KOiK10573
OMAiRENMKEY
OrthoDBiEOG091G0UM8
PhylomeDBiP25153

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

P25153-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTPARRRLM RDFKRLQEDP PTGVSGAPTD NNIMIWNAVI FGPHDTPFED
60 70 80 90 100
GTFKLTIEFT EEYPNKPPTV RFVSKVFHPN VYADGGICLD ILQNRWSPTY
110 120 130 140 150
DVSAILTSIQ SLLSDPNPNS PANSTAAQLY KENRREYEKR VKACVEQSFI

D
Length:151
Mass (Da):17,152
Last modified:June 1, 2001 - v2
Checksum:iCC4B35992E4A9220
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti99T → R in AAA28308 (PubMed:1902572).Curated1
Sequence conflicti99T → R in AAA28309 (PubMed:1902572).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63792 mRNA Translation: AAA28308.1
M64435, M63791 Genomic DNA Translation: AAA28309.1
AE014297 Genomic DNA Translation: AAF52079.1
BT003481 mRNA Translation: AAO39484.1
PIRiA39392
RefSeqiNP_001246916.1, NM_001259987.3
NP_524230.2, NM_079506.4
UniGeneiDm.2229

Genome annotation databases

EnsemblMetazoaiFBtr0078849; FBpp0078490; FBgn0004436
FBtr0306106; FBpp0297243; FBgn0004436
GeneIDi40610
KEGGidme:Dmel_CG2013

Similar proteinsi

Entry informationi

Entry nameiUBCD6_DROME
AccessioniPrimary (citable) accession number: P25153
Secondary accession number(s): Q9VN70
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: June 1, 2001
Last modified: May 23, 2018
This is version 154 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families
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