P25148 (GSPA_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
General stress protein A

Gene names

Name:	gspA
Synonyms:	ywaG
Ordered Locus Names:	BSU38430
ORF Names:	ipa-12d

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Induction	By different stresses such as heat shock and salt stress and by starvation. Ref.4
Sequence similarities	Belongs to the glycosyltransferase 8 family.
Sequence caution	The sequence CAA36721.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Stress response
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring glycosyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	General stress protein A		PRO_0000083867
Experimental info
	Sequence conflict	12	1	C → A AA sequence Ref.4
	Sequence conflict	27	1	S → V AA sequence Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P25148 [UniParc]. Last modified February 1, 1995. Version 2. Checksum: A282BE6E3B6518DE	FASTA	286	33,522
	10 20 30 40 50 60 MRKDEIMHIV SCADDNYARH LGGMFVSLLT NMDQEREVKL YVIDGGIKPD NKKRLEETTL 70 80 90 100 110 120 KFGVPIEFLE VDTNMYEHAV ESSHITKAAY YRISIPDLIK DESIKRMIYI DCDALVLEDI 130 140 150 160 170 180 SKLWDLDIAP YTVAAVEDAG QHERLKEMNV TDTGKYFNSG IMIIDFESWR KQNITEKVIN 190 200 210 220 230 240 FINEHPDEDF LVLHDQDALN AILYDQWYEL HPRWNAQTYI MLKLKTPSTL LGRKQYNETR 250 260 270 280 ENPAIVHFCG GEKPWNSNTK HPYRDEYFHY MSYTKWNTIG NPAINQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A gene encoding a tyrosine tRNA synthetase is located near sacS in Bacillus subtilis." Glaser P., Kunst F., Debarbouille M., Vertes A., Danchin A., Dedonder R. DNA Seq. 1:251-261(1991) [PubMed: 1806041] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"Bacillus subtilis genome project: cloning and sequencing of the 97 kb region from 325 degrees to 333 degrees." Glaser P., Kunst F., Arnaud M., Coudart M.P., Gonzales W., Hullo M.-F., Ionescu M., Lubochinsky B., Marcelino L., Moszer I., Presecan E., Santana M., Schneider E., Schweizer J., Vertes A., Rapoport G., Danchin A. Mol. Microbiol. 10:371-384(1993) [PubMed: 7934828] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"A gene at 333 degrees on the Bacillus subtilis chromosome encodes the newly identified sigma B-dependent general stress protein GspA." Antelmann H., Bernhardt J., Schmid R., Hecker M. J. Bacteriol. 177:3540-3545(1995) [PubMed: 7768864] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-28, CHARACTERIZATION, INDUCTION.
[5]	"Nucleotide sequence of the sacS locus of Bacillus subtilis reveals the presence of two regulatory genes." Zukowski M.M., Miller L., Cosgwell P., Chen K., Aymerich S., Steinmetz M. Gene 90:153-155(1990) [PubMed: 2116367] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 110-286. Strain: QB1072.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X52480 Genomic DNA. Translation: CAA36721.1. Different initiation. X73124 Genomic DNA. Translation: CAA51568.1. AL009126 Genomic DNA. Translation: CAB15869.1. M29333 Genomic DNA. Translation: AAA75337.1.
PIR	S16423.
RefSeq	NP_391722.1. NC_000964.3.
3D structure databases
ProteinModelPortal	P25148.
SMR	P25148. Positions 7-274.
ModBase	Search...
Protein family/group databases
CAZy	GT8. Glycosyltransferase Family 8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000001767; EBBACP00000001767; EBBACG00000001765.
GeneID	937339.
GenomeReviews	Gene locus BSU38430 in contig AL009126_GR.
KEGG	bsu:BSU38430.
NMPDR	fig|224308.1.peg.3848.
PATRIC	18979732. VBIBacSub10457_4028.
Organism-specific databases
GenoList	BSU38430. [Micado]
Phylogenomic databases
GeneTree	EBGT00050000001679.
HOGENOM	HBG364570.
OMA	WKEENIF.
PhylomeDB	P25148.
ProtClustDB	CLSK888110.
Enzyme and pathway databases
BioCyc	BSUB:BSU38430-MONOMER.
Family and domain databases
InterPro	IPR002495. Glyco_trans_8. [Graphical view]
Pfam	PF01501. Glyco_transf_8. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSPA_BACSU

Accession

Primary (citable) accession number: P25148

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	February 1, 1995
Last modified:	January 25, 2012
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program