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Protein

Internalin B

Gene

inlB

Organism
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the entry of Listeria monocytogenes into cells.

Enzyme and pathway databases

BioCyciLMON169963:LMO0434-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Internalin B
Gene namesi
Name:inlB
Ordered Locus Names:lmo0434
OrganismiListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Taxonomic identifieri169963 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
Proteomesi
  • UP000000817 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030Sequence analysisAdd
BLAST
Chaini31 – 630600Internalin BPRO_0000021514Add
BLAST

Proteomic databases

PaxDbiP25147.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
C1QBPQ9MZE03EBI-1379295,EBI-6375765From a different organism.
METP085814EBI-1379295,EBI-1039152From a different organism.

Protein-protein interaction databases

IntActiP25147. 2 interactions.
STRINGi169963.lmo0434.

Structurei

Secondary structure

1
630
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi44 – 474Combined sources
Helixi51 – 6010Combined sources
Beta strandi68 – 703Combined sources
Helixi72 – 765Combined sources
Beta strandi80 – 823Combined sources
Helixi94 – 963Combined sources
Beta strandi102 – 1043Combined sources
Helixi114 – 1163Combined sources
Beta strandi124 – 1263Combined sources
Helixi136 – 1383Combined sources
Beta strandi146 – 1483Combined sources
Helixi158 – 1625Combined sources
Beta strandi168 – 1703Combined sources
Helixi180 – 1845Combined sources
Beta strandi189 – 1924Combined sources
Helixi202 – 2043Combined sources
Beta strandi212 – 2143Combined sources
Helixi224 – 2263Combined sources
Beta strandi233 – 24311Combined sources
Beta strandi251 – 2566Combined sources
Beta strandi262 – 2643Combined sources
Beta strandi270 – 2734Combined sources
Beta strandi277 – 2793Combined sources
Beta strandi282 – 2865Combined sources
Beta strandi288 – 2903Combined sources
Beta strandi292 – 30413Combined sources
Beta strandi307 – 32014Combined sources
Beta strandi322 – 3298Combined sources
Beta strandi332 – 3398Combined sources
Beta strandi355 – 36612Combined sources
Turni372 – 3743Combined sources
Beta strandi382 – 3909Combined sources
Turni406 – 4094Combined sources
Beta strandi411 – 4177Combined sources
Helixi420 – 4223Combined sources
Beta strandi423 – 4275Combined sources
Turni429 – 4324Combined sources
Beta strandi448 – 4514Combined sources
Beta strandi456 – 4583Combined sources
Turni459 – 4613Combined sources
Beta strandi478 – 4836Combined sources
Beta strandi489 – 4979Combined sources
Beta strandi503 – 5053Combined sources
Helixi506 – 5094Combined sources
Beta strandi514 – 5163Combined sources
Beta strandi526 – 5327Combined sources
Beta strandi535 – 5417Combined sources
Turni542 – 5443Combined sources
Beta strandi545 – 5506Combined sources
Helixi551 – 5533Combined sources
Beta strandi555 – 5639Combined sources
Turni565 – 5695Combined sources
Beta strandi570 – 5778Combined sources
Helixi580 – 5823Combined sources
Beta strandi583 – 5864Combined sources
Helixi587 – 5893Combined sources
Beta strandi595 – 6039Combined sources
Beta strandi606 – 6127Combined sources
Beta strandi614 – 6229Combined sources
Helixi623 – 6253Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1D0BX-ray1.86A36-248[»]
1H6TX-ray1.60A36-321[»]
1M9SX-ray2.65A36-630[»]
1OTMX-ray1.93A36-248[»]
1OTNX-ray1.97A36-248[»]
1OTOX-ray1.96A36-248[»]
2UZXX-ray2.80A/C36-321[»]
2UZYX-ray4.00A/C36-321[»]
2WQUX-ray2.60A/B/C/D/E/F36-321[»]
2WQVX-ray2.80A/B36-321[»]
2WQWX-ray2.24A/B36-321[»]
2WQXX-ray2.03A/B36-321[»]
2Y5PX-ray1.30A/B/C/D322-392[»]
2Y5QX-ray3.20A36-392[»]
4AW4X-ray1.93A/B/C36-321[»]
4CILX-ray1.50A93-321[»]
ProteinModelPortaliP25147.
SMRiP25147. Positions 36-320, 391-629.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25147.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 7646LRRNTAdd
BLAST
Repeati77 – 9822LRR 1Add
BLAST
Repeati99 – 12022LRR 2Add
BLAST
Repeati121 – 14222LRR 3Add
BLAST
Repeati143 – 16422LRR 4Add
BLAST
Repeati165 – 18622LRR 5Add
BLAST
Repeati187 – 20822LRR 6Add
BLAST
Repeati209 – 23022LRR 7Add
BLAST
Domaini241 – 33090LRRCTAdd
BLAST
Domaini393 – 46775GW 1PROSITE-ProRule annotationAdd
BLAST
Repeati399 – 465672-1Add
BLAST
Domaini472 – 55079GW 2PROSITE-ProRule annotationAdd
BLAST
Domaini553 – 63078GW 3PROSITE-ProRule annotationAdd
BLAST
Repeati559 – 630722-2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni399 – 6302322 X approximate repeatsAdd
BLAST

Sequence similaritiesi

Contains 3 GW domains.PROSITE-ProRule annotation
Contains 7 LRR (leucine-rich) repeats.Curated
Contains 1 LRRCT domain.Curated
Contains 1 LRRNT domain.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal

Phylogenomic databases

eggNOGiENOG4108NJY. Bacteria.
COG4886. LUCA.
COG5632. LUCA.
KOiK13731.
OMAiKYCLISG.
OrthoDBiEOG6GXTN7.
PhylomeDBiP25147.

Family and domain databases

Gene3Di2.60.40.1220. 1 hit.
3.80.10.10. 1 hit.
InterProiIPR014755. Cu-Rt/internalin_Ig-like.
IPR014756. Ig_E-set.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR025875. Leu-rich_rpt_4.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR013378. Listeria/Bacterioides_rpt.
IPR012569. LRR-contain_adjacent_dom.
IPR025987. SH3-like_dom.
[Graphical view]
PfamiPF09479. Flg_new. 1 hit.
PF12799. LRR_4. 3 hits.
PF08191. LRR_adjacent. 1 hit.
PF13457. SH3_8. 3 hits.
[Graphical view]
SMARTiSM00369. LRR_TYP. 5 hits.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR02543. List_Bact_rpt. 1 hit.
PROSITEiPS51780. GW. 3 hits.
PS51450. LRR. 6 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25147-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKEKHNPRRK YCLISGLAII FSLWIIIGNG AKVQAETITV PTPIKQIFSD
60 70 80 90 100
DAFAETIKDN LKKKSVTDAV TQNELNSIDQ IIANNSDIKS VQGIQYLPNV
110 120 130 140 150
TKLFLNGNKL TDIKPLANLK NLGWLFLDEN KVKDLSSLKD LKKLKSLSLE
160 170 180 190 200
HNGISDINGL VHLPQLESLY LGNNKITDIT VLSRLTKLDT LSLEDNQISD
210 220 230 240 250
IVPLAGLTKL QNLYLSKNHI SDLRALAGLK NLDVLELFSQ ECLNKPINHQ
260 270 280 290 300
SNLVVPNTVK NTDGSLVTPE IISDDGDYEK PNVKWHLPEF TNEVSFIFYQ
310 320 330 340 350
PVTIGKAKAR FHGRVTQPLK EVYTVSYDVD GTVIKTKVEA GTRITAPKPP
360 370 380 390 400
TKQGYVFKGW YTEKNGGHEW NFNTDYMSGN DFTLYAVFKA ETTEKAVNLT
410 420 430 440 450
RYVKYIRGNA GIYKLPREDN SLKQGTLASH RCKALTVDRE ARNGGKLWYR
460 470 480 490 500
LKNIGWTKAE NLSLDRYDKM EYDKGVTAYA RVRNASGNSV WTKPYNTAGA
510 520 530 540 550
KHVNKLSVYQ GKNMRILREA KTPITTWYQF SIGGKVIGWV DTRALNTFYK
560 570 580 590 600
QSMEKPTRLT RYVSANKAGE SYYKVPVADN PVKRGTLAKY KNQKLIVDCQ
610 620 630
ATIEGQLWYR IRTSSTFIGW TKAANLRAQK
Length:630
Mass (Da):71,147
Last modified:December 5, 2001 - v2
Checksum:iCA05F31BEFE9748C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti41 – 411P → S in AAA25290 (PubMed:1905979).Curated
Sequence conflicti49 – 491S → P in AAA25290 (PubMed:1905979).Curated
Sequence conflicti117 – 1171A → T in AAA25290 (PubMed:1905979).Curated
Sequence conflicti132 – 1321V → I in AAA25290 (PubMed:1905979).Curated
Sequence conflicti396 – 3961A → T in AAA25290 (PubMed:1905979).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M67471 Genomic DNA. Translation: AAA25290.1.
AJ012346 Genomic DNA. Translation: CAC20629.1.
AL591975 Genomic DNA. Translation: CAC98513.1.
PIRiAC1129.
C39930.
RefSeqiNP_463963.1. NC_003210.1.
WP_010989463.1. NC_003210.1.

Genome annotation databases

EnsemblBacteriaiCAC98513; CAC98513; CAC98513.
GeneIDi986892.
KEGGilmo:lmo0434.
PATRICi20309923. VBILisMon69206_0447.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M67471 Genomic DNA. Translation: AAA25290.1.
AJ012346 Genomic DNA. Translation: CAC20629.1.
AL591975 Genomic DNA. Translation: CAC98513.1.
PIRiAC1129.
C39930.
RefSeqiNP_463963.1. NC_003210.1.
WP_010989463.1. NC_003210.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1D0BX-ray1.86A36-248[»]
1H6TX-ray1.60A36-321[»]
1M9SX-ray2.65A36-630[»]
1OTMX-ray1.93A36-248[»]
1OTNX-ray1.97A36-248[»]
1OTOX-ray1.96A36-248[»]
2UZXX-ray2.80A/C36-321[»]
2UZYX-ray4.00A/C36-321[»]
2WQUX-ray2.60A/B/C/D/E/F36-321[»]
2WQVX-ray2.80A/B36-321[»]
2WQWX-ray2.24A/B36-321[»]
2WQXX-ray2.03A/B36-321[»]
2Y5PX-ray1.30A/B/C/D322-392[»]
2Y5QX-ray3.20A36-392[»]
4AW4X-ray1.93A/B/C36-321[»]
4CILX-ray1.50A93-321[»]
ProteinModelPortaliP25147.
SMRiP25147. Positions 36-320, 391-629.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP25147. 2 interactions.
STRINGi169963.lmo0434.

Proteomic databases

PaxDbiP25147.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC98513; CAC98513; CAC98513.
GeneIDi986892.
KEGGilmo:lmo0434.
PATRICi20309923. VBILisMon69206_0447.

Phylogenomic databases

eggNOGiENOG4108NJY. Bacteria.
COG4886. LUCA.
COG5632. LUCA.
KOiK13731.
OMAiKYCLISG.
OrthoDBiEOG6GXTN7.
PhylomeDBiP25147.

Enzyme and pathway databases

BioCyciLMON169963:LMO0434-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP25147.

Family and domain databases

Gene3Di2.60.40.1220. 1 hit.
3.80.10.10. 1 hit.
InterProiIPR014755. Cu-Rt/internalin_Ig-like.
IPR014756. Ig_E-set.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR025875. Leu-rich_rpt_4.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR013378. Listeria/Bacterioides_rpt.
IPR012569. LRR-contain_adjacent_dom.
IPR025987. SH3-like_dom.
[Graphical view]
PfamiPF09479. Flg_new. 1 hit.
PF12799. LRR_4. 3 hits.
PF08191. LRR_adjacent. 1 hit.
PF13457. SH3_8. 3 hits.
[Graphical view]
SMARTiSM00369. LRR_TYP. 5 hits.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR02543. List_Bact_rpt. 1 hit.
PROSITEiPS51780. GW. 3 hits.
PS51450. LRR. 6 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Entry of L. monocytogenes into cells is mediated by internalin, a repeat protein reminiscent of surface antigens from Gram-positive cocci."
    Gaillard J.-L., Berche P., Frehel C., Gouin E., Cossart P.
    Cell 65:1127-1141(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: EGD-SmR / Serovar 1/2a.
  2. "Nucleotide sequence of the internalin operon from Listeria monocytogenes EGD."
    Hain T., Pashalidis P., Hudel M., Chakraborty T., Domann E.
    Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: EGD / Serovar 1/2a.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-679 / EGD-e.
  4. "Structure of the inlB leucine-rich repeats, a domain that triggers host cell invasion by the bacterial pathogen L. monocytogenes."
    Marino M., Braun L., Cossart P., Ghosh P.
    Mol. Cell 4:1063-1072(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.86 ANGSTROMS) OF 36-248.
  5. "Structure of the human receptor tyrosine kinase met in complex with the Listeria invasion protein InlB."
    Niemann H.H., Jager V., Butler P.J., van den Heuvel J., Schmidt S., Ferraris D., Gherardi E., Heinz D.W.
    Cell 130:235-246(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 36-321 IN COMPLEX WITH HUMAN MET.

Entry informationi

Entry nameiINLB_LISMO
AccessioniPrimary (citable) accession number: P25147
Secondary accession number(s): Q9EXG1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: December 5, 2001
Last modified: May 11, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.