P25146 (INLA_LISMO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Internalin-A

Gene names

Name:	inlA
Ordered Locus Names:	lmo0433

Organism

Listeria monocytogenes

Taxonomic identifier

1639 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	800 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mediates the entry of Listeria monocytogenes into cells.
Subcellular location	Secreted › cell wall; Peptidoglycan-anchor Ref.8.
Post-translational modification	Proteolytically cleaved between Thr-770 and Gly-771 by sortase A (SrtA). Ref.8 Ref.9
Miscellaneous	The formation of the complex between internalin and human host receptor (CDH1) is calcium-dependent.
Sequence similarities	Contains 15 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 LRRNT domain.
Sequence caution	The sequence AAA25289.1 differs from that shown. Reason: Frameshift at position 56.

Ontologies

Keywords
Cellular component	Cell wall Secreted
Domain	Leucine-rich repeat Repeat Signal
PTM	Peptidoglycan-anchor
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cell surface Inferred from electronic annotation. Source: InterPro cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 35

35

Potential

Chain

36 – 770

735

Internalin-A

PRO_0000005609

Propeptide

771 – 800

30

Removed by sortase A

PRO_0000005610

Regions

Domain

36 – 76

41

LRRNT

Repeat

77 – 98

22

LRR 1

Repeat

99 – 120

22

LRR 2

Repeat

121 – 142

22

LRR 3

Repeat

143 – 164

22

LRR 4

Repeat

165 – 186

22

LRR 5

Repeat

187 – 207

21

LRR 6

Repeat

208 – 229

22

LRR 7

Repeat

230 – 251

22

LRR 8

Repeat

252 – 273

22

LRR 9

Repeat

274 – 295

22

LRR 10

Repeat

296 – 317

22

LRR 11

Repeat

318 – 339

22

LRR 12

Repeat

340 – 361

22

LRR 13

Repeat

362 – 383

22

LRR 14

Repeat

384 – 405

22

LRR 15

Domain

416 – 505

90

LRRCT

Repeat

518 – 587

70

B-1

Repeat

588 – 657

70

B-2

Repeat

658 – 706

49

B-3

Region

518 – 706

189

3 X approximate tandem repeats, type B

Motif

767 – 771

5

LPXTG sorting signal

Amino acid modifications

Modified residue

770

1

Pentaglycyl murein peptidoglycan amidated threonine

Natural variations

Natural variant

3

1

K → R in strain: H4.

Natural variant

19

1

F → L in strain: H4.

Natural variant

51

1

T → A in strain: EGD-SmR / Serovar 1/2a and 10403S.

Natural variant

94

1

V → L in strain: 10403S, EGD-SmR / Serovar 1/2a, F4233 / Serotype 1/2b, F6789 / Serotype 1/2b, F5782 / Serotype 4b and H4.

Natural variant

118

1

N → D in strain: 10403S, EGD-SmR / Serovar 1/2a, F4233 / Serotype 1/2b, F6789 / Serotype 1/2b, F5782 / Serotype 4b and H4.

Natural variant

142

1

T → S in strain: F5782 / Serotype 4b and H4.

Natural variant

187

1

S → N in strain: 10403S, EGD-SmR / Serovar 1/ 2a, F4233 / Serotype 1/2b, F6789 / Serotype 1/2b and H4.

Natural variant

193

1

F → L in strain: F5782 / Serotype 4b, F4233 / Serotype 1/2b and F6789 / Serotype 1/2b.

Natural variant

253

1

L → W in strain: F4233 / Serotype 1/2b and F6789 / Serotype 1/2b.

Natural variant

292

1

S → P in strain: F4233 / Serotype 1/2b and F5782 / Serotype 4b.

Natural variant

292

1

S → R in strain: F6789 / Serotype 1/2b.

Natural variant

321

1

N → S in strain: H4.

Natural variant

381

1

N → S in strain: H4.

Natural variant

416

1

A → E in strain: F4233 / Serotype 1/2b, F6789 / Serotype 1/2b and F5782 / Serotype 4b.

Natural variant

454

1

T → A in strain: EGD-SmR / Serovar 1/2a, 10403S and H4.

Natural variant

474

1

S → N in strain: H4.

Natural variant

476

1

P → S in strain: H4.

Natural variant

500

1

V → A in strain: EGD-SmR / Serovar 1/2a, 10403S and H4.

Natural variant

530

1

H → Y in strain: H4.

Natural variant

558

1

N → D in strain: H4.

Natural variant

573

1

D → E in strain: EGD-SmR / Serovar 1/2a and 10403S.

Natural variant

594

1

A → P in strain: EGD-SmR / Serovar 1/2a and 10403S.

Natural variant

648

1

S → T in strain: H4.

Natural variant

664

1

A → T in strain: H4.

Natural variant

729

1

P → S in strain: H4.

Natural variant

738

1

D → N in strain: H4.

Natural variant

781

1

L → I in strain: H4.

Natural variant

790

1

M → V in strain: H4.

Natural variant

791

1

A → V in strain: 10403S.

Secondary structure

1

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800

Helix Strand Turn

Details...

Helix

44 – 47

4

Helix

51 – 60

10

Helix

72 – 75

4

Beta strand

80 – 82

3

Helix

94 – 96

3

Beta strand

102 – 104

3

Helix

114 – 116

3

Beta strand

124 – 126

3

Helix

136 – 138

3

Beta strand

145 – 148

4

Helix

158 – 160

3

Beta strand

167 – 176

10

Helix

180 – 182

3

Beta strand

190 – 196

7

Helix

201 – 203

3

Beta strand

211 – 213

3

Helix

223 – 227

5

Beta strand

233 – 235

3

Helix

245 – 249

5

Beta strand

255 – 257

3

Helix

267 – 271

5

Beta strand

277 – 279

3

Helix

289 – 291

3

Beta strand

298 – 301

4

Helix

311 – 313

3

Beta strand

320 – 323

4

Helix

333 – 337

5

Beta strand

342 – 345

4

Helix

355 – 359

5

Beta strand

365 – 367

3

Helix

377 – 381

5

Beta strand

387 – 389

3

Helix

399 – 401

3

Beta strand

409 – 411

3

Beta strand

415 – 418

4

Beta strand

429 – 431

3

Beta strand

445 – 447

3

Helix

448 – 450

3

Beta strand

452 – 454

3

Beta strand

457 – 459

3

Beta strand

467 – 479

13

Beta strand

482 – 494

13

Sequences

	Sequence		Length	Mass (Da)
	P25146 [UniParc]. Last modified December 5, 2001. Version 3. Checksum: 4D1B03A5CF7CCDE3	FASTA	800	86,493
	10 20 30 40 50 60 MRKKRYVWLK SILVAILVFG SGVWINTSNG TNAQAATITQ DTPINQIFTD TALAEKMKTV 70 80 90 100 110 120 LGKTNVTDTV SQTDLDQVTT LQADRLGIKS IDGVEYLNNL TQINFSNNQL TDITPLKNLT 130 140 150 160 170 180 KLVDILMNNN QIADITPLAN LTNLTGLTLF NNQITDIDPL KNLTNLNRLE LSSNTISDIS 190 200 210 220 230 240 ALSGLTSLQQ LSFGNQVTDL KPLANLTTLE RLDISSNKVS DISVLAKLTN LESLIATNNQ 250 260 270 280 290 300 ISDITPLGIL TNLDELSLNG NQLKDIGTLA SLTNLTDLDL ANNQISNLAP LSGLTKLTEL 310 320 330 340 350 360 KLGANQISNI SPLAGLTALT NLELNENQLE DISPISNLKN LTYLTLYFNN ISDISPVSSL 370 380 390 400 410 420 TKLQRLFFYN NKVSDVSSLA NLTNINWLSA GHNQISDLTP LANLTRITQL GLNDQAWTNA 430 440 450 460 470 480 PVNYKANVSI PNTVKNVTGA LIAPATISDG GSYTEPDITW NLPSYTNEVS YTFSQPVTIG 490 500 510 520 530 540 KGTTTFSGTV TQPLKAIFNV KFHVDGKETT KEVEAGNLLT EPAKPVKEGH TFVGWFDAQT 550 560 570 580 590 600 GGTKWNFSTD KMPTNDINLY AQFSINSYTA TFDNDGVTTS QTVDYQGLLQ EPTAPTKEGY 610 620 630 640 650 660 TFKGWYDAKT GGDKWDFATS KMPAKNITLY AQYSANSYTA TFDVDGKSTT QAVDYQGLLK 670 680 690 700 710 720 EPKAPTKAGY TFKGWYDEKT DGKKWDFATD KMPANDITLY AQFTKNPVAP PTTGGNTPPT 730 740 750 760 770 780 TNNGGNTTPP SANIPGSDTS NTSTGNSAST TSTMNAYDPY NSKEASLPTT GDSDNALYLL 790 800 LGLLAVGTAM ALTKKARASK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Entry of L. monocytogenes into cells is mediated by internalin, a repeat protein reminiscent of surface antigens from Gram-positive cocci." Gaillard J.-L., Berche P., Frehel C., Gouin E., Cossart P. Cell 65:1127-1141(1991) [PubMed: 1905979] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: EGD-SmR / Serovar 1/2a.
[2]	"Common features of Gram-positive bacterial proteins involved in cell recognition." Dramsi S., Dehoux P., Cossart P. Mol. Microbiol. 9:1119-1121(1993) [PubMed: 7934917] [Abstract] Cited for: SEQUENCE REVISION TO N-TERMINUS. Strain: EGD-SmR / Serovar 1/2a.
[3]	"Nucleotide sequence of the internalin operon from Listeria monocytogenes EGD." Hain T., Pashalidis P., Hudel M., Chakraborty T., Domann E. Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: EGD / Mackaness / Serovar 1/2a.
[4]	Jiang L., Xu J., Chen N., Chen X., Fang W. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 10403S / Serovar 1/2a and H4.
[5]	"Comparative genomics of Listeria species." Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F., Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A., Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E., Dominguez-Bernal G., Duchaud E. , Durant L., Dussurget O., Entian K.-D., Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W., Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U., Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A., Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B., Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N., Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P. Science 294:849-852(2001) [PubMed: 11679669] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-679 / EGD-e / Serovar 1/2a.
[6]	"Identification and characterization of nucleotide sequence differences in three virulence-associated genes of Listeria monocytogenes strains representing clinically important serotypes." Vines A., Swaminathan B. Curr. Microbiol. 36:309-318(1998) [PubMed: 9541569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 73-418. Strain: F4233 / Serotype 1/2b, F5782 / Serotype 4b and F6789 / Serotype 1/2b.
[7]	"Anchor structure of cell wall surface proteins in Listeria monocytogenes." Dhar G., Faull K.F., Schneewind O. Biochemistry 39:3725-3733(2000) [PubMed: 10736172] [Abstract] Cited for: SORTING SIGNAL, CELL WALL ANCHORING. Strain: EGD / Mackaness / Serovar 1/2a.
[8]	"Inactivation of the srtA gene in Listeria monocytogenes inhibits anchoring of surface proteins and affects virulence." Bierne H., Mazmanian S.K., Trost M., Pucciarelli M.G., Liu G., Dehoux P., Jansch L., Garcia-del Portillo F., Schneewind O., Cossart P. Mol. Microbiol. 43:869-881(2002) [PubMed: 11929538] [Abstract] Cited for: PROTEOLYTIC PROCESSING BY SRTA, SUBCELLULAR LOCATION. Strain: ATCC BAA-679 / EGD-e / Serovar 1/2a.
[9]	"Identification of substrates of the Listeria monocytogenes sortases A and B by a non-gel proteomic analysis." Pucciarelli M.G., Calvo E., Sabet C., Bierne H., Cossart P., Garcia-del Portillo F. Proteomics 5:4808-4817(2005) [PubMed: 16247833] [Abstract] Cited for: PROTEOLYTIC PROCESSING BY SRTA. Strain: ATCC BAA-679 / EGD-e / Serovar 1/2a.
[10]	"Structure of internalin, a major invasion protein of Listeria monocytogenes, in complex with its human receptor E-cadherin." Schubert W.-D., Urbanke C., Ziehm T., Beier V., Machner M.P., Domann E., Wehland J., Chakraborty T., Heinz D.W. Cell 111:825-836(2002) [PubMed: 12526809] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 36-496 ALONE AND IN COMPLEX WITH HUMAN CDH1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M67471 Genomic DNA. Translation: AAA25289.1. Frameshift.
AJ012346 Genomic DNA. Translation: CAC20628.1.
DQ132794 Genomic DNA. Translation: AAZ53235.1.
DQ132795 Genomic DNA. Translation: AAZ53236.1.
AL591975 Genomic DNA. Translation: CAC98512.1.
U25448 Genomic DNA. Translation: AAA69530.1.
U25451 Genomic DNA. Translation: AAA69533.1.
U25454 Genomic DNA. Translation: AAA69536.1.

PIR

AB1129.

RefSeq

NP_463962.1. NC_003210.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1O6S	X-ray	1.80	A	36-496	[»]
1O6T	X-ray	1.60	A	36-496	[»]
1O6V	X-ray	1.50	A/B	36-496	[»]
2OMT	X-ray	2.00	A	36-496	[»]
2OMU	X-ray	1.80	A	36-496	[»]
2OMV	X-ray	1.90	A	36-496	[»]
2OMW	X-ray	1.85	A	36-496	[»]
2OMX	X-ray	1.70	A	36-496	[»]
2OMY	X-ray	1.70	A	36-496	[»]
2OMZ	X-ray	1.60	A	36-495	[»]

ProteinModelPortal

P25146.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-35738N.

IntAct

P25146. 1 interaction.

MINT

MINT-254818.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

985151.

GenomeReviews

Gene locus lmo0433 in contig AL591824_GR.

KEGG

lmo:lmo0433.

PATRIC

20309921. VBILisMon69206_0446.

Organism-specific databases

GenoList

LMO0433.

Phylogenomic databases

OMA

WINTSNG.

ProtClustDB

CLSK225140.

Enzyme and pathway databases

BioCyc

LMON169963:LMO0433-MONOMER.

Family and domain databases

InterPro

IPR014755. Cu-Rt/internalin_Ig-like.
IPR019948. Gram-positive_anchor.
IPR014756. Ig_E-set.
IPR001611. Leu-rich_rpt.
IPR013378. Listeria/Bacterioides_rpt.
IPR019931. LPXTG-motif_cell_wall_anchor.
IPR012569. LRR-contain_adjacent_dom.
IPR001899. Surface_protein_Gram_pos_cocci.
[Graphical view]

Gene3D

G3DSA:2.60.40.1220. Cu-resist/internalin_Ig-like. 1 hit.

KO

K13730.

Pfam

PF09479. Flg_new. 3 hits.
PF00746. Gram_pos_anchor. 1 hit.
PF08191. LRR_adjacent. 1 hit.
[Graphical view]

SUPFAM

SSF81296. Ig_E-set. 1 hit.

TIGRFAMs

TIGR02543. List_Bact_rpt. 3 hits.
TIGR01167. LPXTG_anchor. 1 hit.

PROSITE

PS50847. GRAM_POS_ANCHORING. 1 hit.
PS51450. LRR. 14 hits.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

INLA_LISMO

Accession

Primary (citable) accession number: P25146
Secondary accession number(s): Q45GD5 , Q45GD6, Q48748, Q48749, Q48750, Q48752, Q9EXG2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	December 5, 2001
Last modified:	January 25, 2012
This is version 109 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program