P25123 (GBRB_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-aminobutyric acid receptor subunit beta

Alternative name(s):
GABA(A) receptor subunit beta
Protein cyclodiene resistance

Gene names

Name:	Rdl
ORF Names:	CG10537

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	606 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	GABA, an inhibitory neurotransmitter, mediates neuronal inhibition by binding to the GABA receptor and opening an integral chloride channel.
Subcellular location	Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
Miscellaneous	Resistance is thought to be due to insensitivity of the cyclodiene/picrotoxinin binding site on the GABA(A) receptor-chloride ionophore complex.
Sequence similarities	Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. [View classification]
RNA editing	Edited at positions 122, 283, 294 and 360. Partially edited. Ref.1 Ref.2 Ref.5 Ref.7
Sequence caution	The sequence AAK92842.1 differs from that shown. Reason: Frameshift at position 448.

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Coding sequence diversity	Alternative splicing RNA editing
Domain	Signal Transmembrane Transmembrane helix
Ligand	Chloride
Molecular function	Chloride channel Ion channel Ligand-gated ion channel Receptor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ion transport Inferred from direct assay Ref.2. Source: UniProtKB negative regulation of synaptic transmission Inferred from mutant phenotype PubMed 12805302. Source: FlyBase regulation of circadian sleep/wake cycle Inferred from mutant phenotype PubMed 19230663. Source: FlyBase regulation of olfactory learning Inferred from mutant phenotype PubMed 18093529. Source: FlyBase response to mechanical stimulus Inferred from genetic interaction PubMed 16943550. Source: FlyBase sleep Inferred from mutant phenotype PubMed 19038223. Source: FlyBase
Cellular_component	axon Inferred from direct assay PubMed 18093529. Source: FlyBase cell junction Inferred from electronic annotation. Source: UniProtKB-KW chloride channel complex Inferred from electronic annotation. Source: UniProtKB-KW dendrite Inferred from direct assay PubMed 18093529. Source: FlyBase integral to membrane Non-traceable author statement Ref.2. Source: UniProtKB plasma membrane Inferred from sequence or structural similarity Ref.2. Source: FlyBase postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GABA-A receptor activity Traceable author statement PubMed 12386938. Source: FlyBase chloride channel activity Inferred from electronic annotation. Source: UniProtKB-KW extracellular ligand-gated ion channel activity Inferred from electronic annotation. Source: InterPro neurotransmitter receptor activity Inferred from direct assay Ref.2. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform C (identifier: P25123-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform A (identifier: P25123-2)

The sequence of this isoform differs from the canonical sequence as follows:
     97-101: VSEVL → LSEVK
     216-248: FWRDGLSSVGMSSEVELPQFRVLGHRQRATEIN → KWNEGPNSVGVSSEVSLPQFKVLGHRQRAMEIS

Isoform B (identifier: P25123-3)

The sequence of this isoform differs from the canonical sequence as follows:
     97-101: VSEVL → LSEVK
     449-470: SNRPIGFSNIQQNVGTRGCSIV → M

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 44	44	Potential
	Chain	45 – 606	562	Gamma-aminobutyric acid receptor subunit beta		PRO_0000000452
Regions
	Topological domain	45 – 268	224	Extracellular Potential
	Transmembrane	269 – 291	23	Helical; Potential
	Transmembrane	297 – 316	20	Helical; Potential
	Transmembrane	333 – 356	24	Helical; Potential
	Topological domain	357 – 568	212	Cytoplasmic Potential
	Transmembrane	569 – 590	22	Helical; Potential
	Compositional bias	499 – 535	37	Gly-rich
Amino acid modifications
	Glycosylation	58	1	N-linked (GlcNAc...) Potential
	Glycosylation	253	1	N-linked (GlcNAc...) Potential
	Disulfide bond	185 ↔ 199		By similarity
Natural variations
	Alternative sequence	97 – 101	5	VSEVL → LSEVK in isoform A and isoform B.		VSP_018587
	Alternative sequence	216 – 248	33	FWRDG…ATEIN → KWNEGPNSVGVSSEVSLPQF KVLGHRQRAMEIS in isoform A.		VSP_018588
	Alternative sequence	449 – 470	22	SNRPI…GCSIV → M in isoform B.		VSP_018589
	Natural variant	122	1	R → G in RNA edited version.
	Natural variant	283	1	I → V in RNA edited version.
	Natural variant	294	1	N → D in RNA edited version.
	Natural variant	360	1	M → V in RNA edited version.
Experimental info
	Sequence conflict	38	1	H → N in AAA19249. Ref.2
	Sequence conflict	68	1	V → I in AAA19249. Ref.2
	Sequence conflict	428	1	G → R in AAA19249. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform C [UniParc]. Last modified May 30, 2006. Version 3. Checksum: 9D4905CD066CBA03	FASTA	606	65,762
	10 20 30 40 50 60 MSDSKMDKLA RMAPLPRTPL LTIWLAINMA LIAQETGHKR IHTVQAATGG GSMLGDVNIS 70 80 90 100 110 120 AILDSFSVSY DKRVRPNYGG PPVEVGVTMY VLSISSVSEV LMDFTLDFYF RQFWTDPRLA 130 140 150 160 170 180 YRKRPGVETL SVGSEFIKNI WVPDTFFVNE KQSYFHIATT SNEFIRVHHS GSITRSIRLT 190 200 210 220 230 240 ITASCPMNLQ YFPMDRQLCH IEIESFGYTM RDIRYFWRDG LSSVGMSSEV ELPQFRVLGH 250 260 270 280 290 300 RQRATEINLT TGNYSRLACE IQFVRSMGYY LIQIYIPSGL IVIISWVSFW LNRNATPARV 310 320 330 340 350 360 ALGVTTVLTM TTLMSSTNAA LPKISYVKSI DVYLGTCFVM VFASLLEYAT VGYMAKRIQM 370 380 390 400 410 420 RKQRFMAIQK IAEQKKQQLD GANQQQANPN PNANVGGPGG VGVGPGGPGG PGGGVNVGVG 430 440 450 460 470 480 MGMGPEHGHG HGHHAHSHGH PHAPKQTVSN RPIGFSNIQQ NVGTRGCSIV GPLFQEVRFK 490 500 510 520 530 540 VHDPKAHSKG GTLENTVNGG RGGPQSHGPG PGQGGGPPGG GGGGGGGGGP PEGGGDPEAA 550 560 570 580 590 600 VPAHLLHPGK VKKDINKLLG ITPSDIDKYS RIVFPVCFVC FNLMYWIIYL HVSDVVADDL VLLGEE
	Isoform A [UniParc]. Checksum: 25BDDCB03DAD54F8	FASTA	606	65,656
	10 20 30 40 50 60 MSDSKMDKLA RMAPLPRTPL LTIWLAINMA LIAQETGHKR IHTVQAATGG GSMLGDVNIS 70 80 90 100 110 120 AILDSFSVSY DKRVRPNYGG PPVEVGVTMY VLSISSLSEV KMDFTLDFYF RQFWTDPRLA 130 140 150 160 170 180 YRKRPGVETL SVGSEFIKNI WVPDTFFVNE KQSYFHIATT SNEFIRVHHS GSITRSIRLT 190 200 210 220 230 240 ITASCPMNLQ YFPMDRQLCH IEIESFGYTM RDIRYKWNEG PNSVGVSSEV SLPQFKVLGH 250 260 270 280 290 300 RQRAMEISLT TGNYSRLACE IQFVRSMGYY LIQIYIPSGL IVIISWVSFW LNRNATPARV 310 320 330 340 350 360 ALGVTTVLTM TTLMSSTNAA LPKISYVKSI DVYLGTCFVM VFASLLEYAT VGYMAKRIQM 370 380 390 400 410 420 RKQRFMAIQK IAEQKKQQLD GANQQQANPN PNANVGGPGG VGVGPGGPGG PGGGVNVGVG 430 440 450 460 470 480 MGMGPEHGHG HGHHAHSHGH PHAPKQTVSN RPIGFSNIQQ NVGTRGCSIV GPLFQEVRFK 490 500 510 520 530 540 VHDPKAHSKG GTLENTVNGG RGGPQSHGPG PGQGGGPPGG GGGGGGGGGP PEGGGDPEAA 550 560 570 580 590 600 VPAHLLHPGK VKKDINKLLG ITPSDIDKYS RIVFPVCFVC FNLMYWIIYL HVSDVVADDL VLLGEE
	Isoform B [UniParc]. Checksum: A6A0BAA0BE9B8407	FASTA	585	63,593
	10 20 30 40 50 60 MSDSKMDKLA RMAPLPRTPL LTIWLAINMA LIAQETGHKR IHTVQAATGG GSMLGDVNIS 70 80 90 100 110 120 AILDSFSVSY DKRVRPNYGG PPVEVGVTMY VLSISSLSEV KMDFTLDFYF RQFWTDPRLA 130 140 150 160 170 180 YRKRPGVETL SVGSEFIKNI WVPDTFFVNE KQSYFHIATT SNEFIRVHHS GSITRSIRLT 190 200 210 220 230 240 ITASCPMNLQ YFPMDRQLCH IEIESFGYTM RDIRYFWRDG LSSVGMSSEV ELPQFRVLGH 250 260 270 280 290 300 RQRATEINLT TGNYSRLACE IQFVRSMGYY LIQIYIPSGL IVIISWVSFW LNRNATPARV 310 320 330 340 350 360 ALGVTTVLTM TTLMSSTNAA LPKISYVKSI DVYLGTCFVM VFASLLEYAT VGYMAKRIQM 370 380 390 400 410 420 RKQRFMAIQK IAEQKKQQLD GANQQQANPN PNANVGGPGG VGVGPGGPGG PGGGVNVGVG 430 440 450 460 470 480 MGMGPEHGHG HGHHAHSHGH PHAPKQTVMG PLFQEVRFKV HDPKAHSKGG TLENTVNGGR 490 500 510 520 530 540 GGPQSHGPGP GQGGGPPGGG GGGGGGGGPP EGGGDPEAAV PAHLLHPGKV KKDINKLLGI 550 560 570 580 TPSDIDKYSR IVFPVCFVCF NLMYWIIYLH VSDVVADDLV LLGEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and transformation of cyclodiene resistance in Drosophila: an invertebrate gamma-aminobutyric acid subtype A receptor locus." ffrench-Constant R.H., Mortlock D.P., Shaffer C.D., Macintyre R.J., Roush R.T. Proc. Natl. Acad. Sci. U.S.A. 88:7209-7213(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM C), RNA EDITING OF POSITION 283. Tissue: Embryo.
[2]	"Cloning and functional expression of a Drosophila gamma-aminobutyric acid receptor." Chen R., Belelli D., Reyes A., Lambert J.J., Peters J.A., Lan N.C. Proc. Natl. Acad. Sci. U.S.A. 91:6069-6073(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), RNA EDITING OF POSITIONS 122; 283 AND 360. Tissue: Head.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B), RNA EDITING OF POSITION 283. Strain: Berkeley. Tissue: Head.
[6]	"Drosophila gamma-aminobutyric acid receptor gene Rdl shows extensive alternative splicing." ffrench-Constant R.H., Rocheleau T.A. J. Neurochem. 60:2323-2326(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 70-113 (ISOFORMS A/B).
[7]	"Nervous system targets of RNA editing identified by comparative genomics." Hoopengardner B., Bhalla T., Staber C., Reenan R. Science 301:832-836(2003) [PubMed] [Europe PMC] [Abstract] Cited for: RNA EDITING.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M69057 mRNA. Translation: AAA28556.1. M69057 mRNA. Translation: AAA28557.1. Sequence problems. M69057 mRNA. Translation: AAA28558.1. Sequence problems. U02042 mRNA. Translation: AAA19249.1. AE014296 Genomic DNA. Translation: AAF50311.1. AE014296 Genomic DNA. Translation: AAN11988.1. AE014296 Genomic DNA. Translation: AAN11989.1. AY051418 mRNA. Translation: AAK92842.1. Frameshift. S61113 mRNA. Translation: AAB26669.1.
PIR	A41145.
RefSeq	NP_001261616.1. NM_001274687.1. NP_523991.2. NM_079267.4. NP_729461.1. NM_168321.3. NP_729462.2. NM_168322.3.
3D structure databases
ProteinModelPortal	P25123.
SMR	P25123. Positions 66-386.
ModBase	Search...
Proteomic databases
PaxDb	P25123.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0076536; FBpp0076263; FBgn0004244.
GeneID	39054.
KEGG	dme:Dmel_CG10537.
Organism-specific databases
CTD	39054.
FlyBase	FBgn0004244. Rdl.
Phylogenomic databases
eggNOG	NOG265706.
GeneTree	ENSGT00630000089685.
InParanoid	P25123.
KO	K05195.
OMA	GGMFGDV.
OrthoDB	EOG44B8HM.
PhylomeDB	P25123.
Gene expression databases
Bgee	P25123.
GermOnline	CG10537. Drosophila melanogaster.
Family and domain databases
Gene3D	2.70.170.10. 1 hit.
InterPro	IPR006028. GABAA_rcpt. IPR002289. GABAAb_rcpt. IPR006202. Neur_chan_lig-bd. IPR006201. Neur_channel. IPR006029. Neurotrans-gated_channel_TM. IPR018000. Neurotransmitter_ion_chnl_CS. [Graphical view]
PANTHER	PTHR18945. PTHR18945. 1 hit.
Pfam	PF02931. Neur_chan_LBD. 1 hit. PF02932. Neur_chan_memb. 1 hit. [Graphical view]
PRINTS	PR01160. GABAARBETA. PR00253. GABAARECEPTR. PR00252. NRIONCHANNEL.
SUPFAM	SSF90112. Neu_channel_TM. 1 hit. SSF63712. Neur_chan_LBD. 1 hit.
TIGRFAMs	TIGR00860. LIC. 1 hit.
PROSITE	PS00236. NEUROTR_ION_CHANNEL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	Rdl. drosophila.
GenomeRNAi	39054.
NextBio	811688.

Entry information

Entry name

GBRB_DROME

Accession

Primary (citable) accession number: P25123
Secondary accession number(s): P92138 , Q24561, Q26302, Q7JPX8, Q8IQB5, Q8IQB6, Q961R4, Q9TX51, Q9VSV0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 30, 2006
Last modified:	May 1, 2013
This is version 119 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project