P25118 (TNR1A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tumor necrosis factor receptor superfamily member 1A

Alternative name(s):
Tumor necrosis factor receptor 1
Short name=TNF-R1
Tumor necrosis factor receptor type I
Short name=TNF-RI
Short name=TNFR-I
p55
p60
CD_antigen=CD120a

Gene names

Name:	Tnfrsf1a
Synonyms:	Tnfr-1, Tnfr1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	454 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis By similarity.
Subunit structure	Binding of TNF to the extracellular domain leads to homotrimerization. The aggregated death domains provide a novel molecular interface that interacts specifically with the death domain of TRADD. Various TRADD-interacting proteins such as TRAFS, RIPK1 and possibly FADD, are recruited to the complex by their association with TRADD. This complex activates at least two distinct signaling cascades, apoptosis and NF-kappa-B signaling. Interacts with BAG4, BRE, FEM1B, GRB2, SQSTM1 and TRPC4AP. Interacts with DAB2IP By similarity.
Subcellular location	Cell membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein By similarity.
Domain	Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP.
Sequence similarities	Contains 1 death domain. Contains 4 TNFR-Cys repeats.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Cell membrane Golgi apparatus Membrane
Domain	Repeat Signal Transmembrane Transmembrane helix
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW cellular response to estradiol stimulus Inferred from electronic annotation. Source: Compara cellular response to mechanical stimulus Inferred from electronic annotation. Source: Compara defense response to bacterium Inferred from mutant phenotype PubMed 9324362. Source: MGI diterpenoid metabolic process Inferred from electronic annotation. Source: Compara flavonoid metabolic process Inferred from electronic annotation. Source: Compara inflammatory response Inferred from mutant phenotype PubMed 9551933PubMed 9843922. Source: MGI negative regulation of gene expression Inferred from electronic annotation. Source: Compara negative regulation of inflammatory response Inferred from electronic annotation. Source: Compara negative regulation of interleukin-6 production Inferred from electronic annotation. Source: Compara positive regulation of I-kappaB kinase/NF-kappaB cascade Inferred from electronic annotation. Source: Compara positive regulation of angiogenesis Inferred from electronic annotation. Source: Compara positive regulation of inflammatory response Inferred from mutant phenotype PubMed 11588035. Source: MGI positive regulation of protein import into nucleus, translocation Inferred from electronic annotation. Source: Compara positive regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype PubMed 11588035. Source: MGI positive regulation of tumor necrosis factor production Inferred from electronic annotation. Source: Compara positive regulation of tyrosine phosphorylation of Stat1 protein Inferred from electronic annotation. Source: Compara prostaglandin metabolic process Traceable author statement PubMed 8387893. Source: MGI protein heterooligomerization Inferred from electronic annotation. Source: Compara regulation of apoptotic process Inferred from electronic annotation. Source: Compara response to alkaloid Inferred from electronic annotation. Source: Compara response to amino acid stimulus Inferred from electronic annotation. Source: Compara response to ethanol Inferred from electronic annotation. Source: Compara response to hypoxia Inferred from electronic annotation. Source: Compara response to lipopolysaccharide Inferred from electronic annotation. Source: Compara tetrapyrrole metabolic process Inferred from electronic annotation. Source: Compara
Cellular_component	Golgi membrane Inferred from sequence or structural similarity. Source: UniProtKB axon Inferred from electronic annotation. Source: Compara cell surface Inferred from direct assay PubMed 21410936. Source: BHF-UCL extracellular space Inferred from electronic annotation. Source: Compara integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane raft Inferred from direct assay PubMed 17010968. Source: BHF-UCL nucleus Inferred from electronic annotation. Source: Compara plasma membrane Traceable author statement PubMed 9551933. Source: MGI protein complex Inferred from electronic annotation. Source: Compara
Molecular_function	tumor necrosis factor-activated receptor activity Inferred from mutant phenotype PubMed 11588035. Source: MGI
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Slc39a7	Q31125	3	EBI-518014,EBI-644519

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 454	433	Tumor necrosis factor receptor superfamily member 1A		PRO_0000034545
Regions
	Topological domain	22 – 212	191	Extracellular Potential
	Transmembrane	213 – 235	23	Helical; Potential
	Topological domain	236 – 454	219	Cytoplasmic Potential
	Repeat	43 – 82	40	TNFR-Cys 1
	Repeat	83 – 125	43	TNFR-Cys 2
	Repeat	126 – 166	41	TNFR-Cys 3
	Repeat	167 – 196	30	TNFR-Cys 4
	Domain	356 – 441	86	Death
	Region	339 – 349	11	N-SMase activation domain (NSD)
Amino acid modifications
	Glycosylation	54	1	N-linked (GlcNAc...) Potential
	Glycosylation	151	1	N-linked (GlcNAc...) Potential
	Glycosylation	202	1	N-linked (GlcNAc...) Potential
	Disulfide bond	44 ↔ 58		By similarity
	Disulfide bond	59 ↔ 72		By similarity
	Disulfide bond	62 ↔ 81		By similarity
	Disulfide bond	84 ↔ 99		By similarity
	Disulfide bond	102 ↔ 117		By similarity
	Disulfide bond	105 ↔ 125		By similarity
	Disulfide bond	127 ↔ 143		By similarity
	Disulfide bond	146 ↔ 158		By similarity
	Disulfide bond	149 ↔ 166		By similarity
	Disulfide bond	168 ↔ 179		By similarity
	Disulfide bond	182 ↔ 195		By similarity
	Disulfide bond	185 ↔ 191		By similarity
Experimental info
	Sequence conflict	394	1	R → G in AAA40465. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P25118 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 0710C2E8C3C2B6D9	FASTA	454	50,130
	10 20 30 40 50 60 MGLPTVPGLL LSLVLLALLM GIHPSGVTGL VPSLGDREKR DSLCPQGKYV HSKNNSICCT 70 80 90 100 110 120 KCHKGTYLVS DCPSPGRDTV CRECEKGTFT ASQNYLRQCL SCKTCRKEMS QVEISPCQAD 130 140 150 160 170 180 KDTVCGCKEN QFQRYLSETH FQCVDCSPCF NGTVTIPCKE TQNTVCNCHA GFFLRESECV 190 200 210 220 230 240 PCSHCKKNEE CMKLCLPPPL ANVTNPQDSG TAVLLPLVIL LGLCLLSFIF ISLMCRYPRW 250 260 270 280 290 300 RPEVYSIICR DPVPVKEEKA GKPLTPAPSP AFSPTSGFNP TLGFSTPGFS SPVSSTPISP 310 320 330 340 350 360 IFGPSNWHFM PPVSEVVPTQ GADPLLYESL CSVPAPTSVQ KWEDSAHPQR PDNADLAILY 370 380 390 400 410 420 AVVDGVPPAR WKEFMRFMGL SEHEIERLEM QNGRCLREAQ YSMLEAWRRR TPRHEDTLEV 430 440 450 VGLVLSKMNL AGCLENILEA LRNPAPSSTT RLPR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of cDNAs for two distinct murine tumor necrosis factor receptors demonstrate one receptor is species specific." Lewis M., Tartaglia L.A., Lee A., Bennett G.L., Rice G.C., Wong G.H., Chen E.Y., Goeddel D.V. Proc. Natl. Acad. Sci. U.S.A. 88:2830-2834(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Molecular cloning and expression of the type 1 and type 2 murine receptors for tumor necrosis factor." Goodwin R.G., Anderson D., Jerzy R., Davis T., Brannan C.I., Copeland N.G., Jenkins N.A., Smith C.A. Mol. Cell. Biol. 11:3020-3026(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Cloning, expression and cross-linking analysis of the murine p55 tumor necrosis factor receptor." Barrett K., Taylor-Fishwick D.A., Cope A.P., Kissonerghis A.M., Gray P.W., Feldmann M., Foxwell B.M.J. Eur. J. Immunol. 21:1649-1656(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Molecular cloning and expression of the mouse Tnf receptor type b." Rothe J.G., Brockhaus M., Gentz R., Lesslauer W. Immunogenetics 34:338-340(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Spleen.
[5]	"Nucleotide sequence of the TNF type I receptor from a mouse endothelioma cell line." Bebo B.F., Linthicum D.S. Immunogenetics 39:450-451(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[6]	"Genomic organization and promoter function of the murine tumor necrosis factor receptor beta gene." Rothe J., Bluethmann H., Gentz R., Lesslauer W., Steinmetz M. Mol. Immunol. 30:165-175(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C3H/He. Tissue: Mesenchymal cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M60468 mRNA. Translation: AAA39751.1. M59377 mRNA. Translation: AAA40464.1. X59238 mRNA. Translation: CAA41922.1. X57796 mRNA. Translation: CAA40936.1. L26349 mRNA. Translation: AAA59361.1. M76656, M88067, M76655 Genomic DNA. Translation: AAA40465.1. BC004599 mRNA. Translation: AAH04599.1. BC052675 mRNA. Translation: AAH52675.1.
IPI	IPI00317228.
PIR	GQMST1. A38634.
RefSeq	NP_035739.2. NM_011609.4.
UniGene	Mm.1258. Mm.474976.
3D structure databases
ProteinModelPortal	P25118.
SMR	P25118. Positions 41-197, 357-441.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-34532N.
IntAct	P25118. 19 interactions.
MINT	MINT-2631249.
PTM databases
PhosphoSite	P25118.
Proteomic databases
PaxDb	P25118.
PRIDE	P25118.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000032491; ENSMUSP00000032491; ENSMUSG00000030341.
GeneID	21937.
KEGG	mmu:21937.
Organism-specific databases
CTD	7132.
MGI	MGI:1314884. Tnfrsf1a.
Phylogenomic databases
eggNOG	NOG39168.
HOGENOM	HOG000133001.
HOVERGEN	HBG058842.
InParanoid	P25118.
KO	K03158.
OMA	LCPQGKY.
OrthoDB	EOG4Z8XWT.
Gene expression databases
ArrayExpress	P25118.
Bgee	P25118.
Genevestigator	P25118.
GermOnline	ENSMUSG00000030341. Mus musculus.
Family and domain databases
Gene3D	1.10.533.10. 1 hit.
InterPro	IPR011029. DEATH-like_dom. IPR000488. Death_domain. IPR001368. TNFR/NGFR_Cys_rich_reg. IPR020419. TNFR_1A. [Graphical view]
Pfam	PF00531. Death. 1 hit. PF00020. TNFR_c6. 3 hits. [Graphical view]
PRINTS	PR01918. TNFACTORR1A.
SMART	SM00005. DEATH. 1 hit. SM00208. TNFR. 4 hits. [Graphical view]
SUPFAM	SSF47986. DEATH_like. 1 hit.
PROSITE	PS50017. DEATH_DOMAIN. 1 hit. PS00652. TNFR_NGFR_1. 3 hits. PS50050. TNFR_NGFR_2. 3 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	301546.
SOURCE	Search...

Entry information

Entry name

TNR1A_MOUSE

Accession

Primary (citable) accession number: P25118

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	April 3, 2013
This is version 134 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program