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P25118 (TNR1A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 143. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tumor necrosis factor receptor superfamily member 1A
Alternative name(s):
Tumor necrosis factor receptor 1
Short name=TNF-R1
Tumor necrosis factor receptor type I
Short name=TNF-RI
Short name=TNFR-I
p55
p60
CD_antigen=CD120a
Gene names
Name:Tnfrsf1a
Synonyms:Tnfr-1, Tnfr1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length454 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis By similarity.

Subunit structure

Binding of TNF to the extracellular domain leads to homotrimerization. The aggregated death domains provide a novel molecular interface that interacts specifically with the death domain of TRADD. Various TRADD-interacting proteins such as TRAFS, RIPK1 and possibly FADD, are recruited to the complex by their association with TRADD. This complex activates at least two distinct signaling cascades, apoptosis and NF-kappa-B signaling. Interacts with BAG4, BRE, FEM1B, GRB2, SQSTM1 and TRPC4AP. Interacts with DAB2IP By similarity.

Subcellular location

Cell membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein By similarity.

Domain

Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP.

Sequence similarities

Contains 1 death domain.

Contains 4 TNFR-Cys repeats.

Ontologies

Keywords
   Biological processApoptosis
   Cellular componentCell membrane
Golgi apparatus
Membrane
   DomainRepeat
Signal
Transmembrane
Transmembrane helix
   Molecular functionReceptor
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell surface receptor signaling pathway

Inferred from mutant phenotype PubMed 9551933. Source: MGI

cellular response to estradiol stimulus

Inferred from electronic annotation. Source: Ensembl

cellular response to mechanical stimulus

Inferred from electronic annotation. Source: Ensembl

cytokine-mediated signaling pathway

Inferred from mutant phenotype PubMed 11588035. Source: MGI

defense response

Inferred from mutant phenotype PubMed 8387893. Source: MGI

defense response to bacterium

Inferred from mutant phenotype PubMed 9324362. Source: MGI

inflammatory response

Inferred from mutant phenotype PubMed 9551933PubMed 9843922. Source: MGI

intrinsic apoptotic signaling pathway in response to DNA damage

Inferred from mutant phenotype PubMed 17242187. Source: MGI

negative regulation of apoptotic process

Inferred from electronic annotation. Source: Ensembl

negative regulation of gene expression

Inferred from electronic annotation. Source: Ensembl

negative regulation of inflammatory response

Inferred from electronic annotation. Source: Ensembl

negative regulation of interleukin-6 production

Inferred from electronic annotation. Source: Ensembl

positive regulation of I-kappaB kinase/NF-kappaB signaling

Inferred from electronic annotation. Source: Ensembl

positive regulation of angiogenesis

Inferred from electronic annotation. Source: Ensembl

positive regulation of inflammatory response

Inferred from mutant phenotype PubMed 11588035. Source: MGI

positive regulation of protein import into nucleus, translocation

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype PubMed 11588035. Source: MGI

positive regulation of tumor necrosis factor production

Inferred from electronic annotation. Source: Ensembl

positive regulation of tyrosine phosphorylation of Stat1 protein

Inferred from electronic annotation. Source: Ensembl

prostaglandin metabolic process

Traceable author statement PubMed 8387893. Source: MGI

protein heterooligomerization

Inferred from electronic annotation. Source: Ensembl

response to alkaloid

Inferred from electronic annotation. Source: Ensembl

response to amino acid

Inferred from electronic annotation. Source: Ensembl

response to ethanol

Inferred from electronic annotation. Source: Ensembl

response to hypoxia

Inferred from electronic annotation. Source: Ensembl

response to lipopolysaccharide

Inferred from electronic annotation. Source: Ensembl

tetrapyrrole metabolic process

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentGolgi membrane

Inferred from sequence or structural similarity. Source: UniProtKB

axon

Inferred from electronic annotation. Source: Ensembl

cell surface

Inferred from direct assay PubMed 21410936. Source: BHF-UCL

extracellular space

Inferred from electronic annotation. Source: Ensembl

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

membrane raft

Inferred from direct assay PubMed 17010968. Source: BHF-UCL

nucleus

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Traceable author statement PubMed 9551933. Source: MGI

receptor complex

Inferred from sequence orthology PubMed 23382219. Source: MGI

   Molecular_functiontumor necrosis factor-activated receptor activity

Inferred from mutant phenotype PubMed 11588035. Source: MGI

Complete GO annotation...

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 454433Tumor necrosis factor receptor superfamily member 1A
PRO_0000034545

Regions

Topological domain22 – 212191Extracellular Potential
Transmembrane213 – 23523Helical; Potential
Topological domain236 – 454219Cytoplasmic Potential
Repeat43 – 8240TNFR-Cys 1
Repeat83 – 12543TNFR-Cys 2
Repeat126 – 16641TNFR-Cys 3
Repeat167 – 19630TNFR-Cys 4
Domain356 – 44186Death
Region339 – 34911N-SMase activation domain (NSD)

Amino acid modifications

Glycosylation541N-linked (GlcNAc...) Potential
Glycosylation1511N-linked (GlcNAc...) Potential
Glycosylation2021N-linked (GlcNAc...) Potential
Disulfide bond44 ↔ 58 By similarity
Disulfide bond59 ↔ 72 By similarity
Disulfide bond62 ↔ 81 By similarity
Disulfide bond84 ↔ 99 By similarity
Disulfide bond102 ↔ 117 By similarity
Disulfide bond105 ↔ 125 By similarity
Disulfide bond127 ↔ 143 By similarity
Disulfide bond146 ↔ 158 By similarity
Disulfide bond149 ↔ 166 By similarity
Disulfide bond168 ↔ 179 By similarity
Disulfide bond182 ↔ 195 By similarity
Disulfide bond185 ↔ 191 By similarity

Experimental info

Sequence conflict3941R → G in AAA40465. Ref.6

Sequences

Sequence LengthMass (Da)Tools
P25118 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: 0710C2E8C3C2B6D9

FASTA45450,130
        10         20         30         40         50         60 
MGLPTVPGLL LSLVLLALLM GIHPSGVTGL VPSLGDREKR DSLCPQGKYV HSKNNSICCT 

        70         80         90        100        110        120 
KCHKGTYLVS DCPSPGRDTV CRECEKGTFT ASQNYLRQCL SCKTCRKEMS QVEISPCQAD 

       130        140        150        160        170        180 
KDTVCGCKEN QFQRYLSETH FQCVDCSPCF NGTVTIPCKE TQNTVCNCHA GFFLRESECV 

       190        200        210        220        230        240 
PCSHCKKNEE CMKLCLPPPL ANVTNPQDSG TAVLLPLVIL LGLCLLSFIF ISLMCRYPRW 

       250        260        270        280        290        300 
RPEVYSIICR DPVPVKEEKA GKPLTPAPSP AFSPTSGFNP TLGFSTPGFS SPVSSTPISP 

       310        320        330        340        350        360 
IFGPSNWHFM PPVSEVVPTQ GADPLLYESL CSVPAPTSVQ KWEDSAHPQR PDNADLAILY 

       370        380        390        400        410        420 
AVVDGVPPAR WKEFMRFMGL SEHEIERLEM QNGRCLREAQ YSMLEAWRRR TPRHEDTLEV 

       430        440        450 
VGLVLSKMNL AGCLENILEA LRNPAPSSTT RLPR 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and expression of cDNAs for two distinct murine tumor necrosis factor receptors demonstrate one receptor is species specific."
Lewis M., Tartaglia L.A., Lee A., Bennett G.L., Rice G.C., Wong G.H., Chen E.Y., Goeddel D.V.
Proc. Natl. Acad. Sci. U.S.A. 88:2830-2834(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Molecular cloning and expression of the type 1 and type 2 murine receptors for tumor necrosis factor."
Goodwin R.G., Anderson D., Jerzy R., Davis T., Brannan C.I., Copeland N.G., Jenkins N.A., Smith C.A.
Mol. Cell. Biol. 11:3020-3026(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Cloning, expression and cross-linking analysis of the murine p55 tumor necrosis factor receptor."
Barrett K., Taylor-Fishwick D.A., Cope A.P., Kissonerghis A.M., Gray P.W., Feldmann M., Foxwell B.M.J.
Eur. J. Immunol. 21:1649-1656(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"Molecular cloning and expression of the mouse Tnf receptor type b."
Rothe J.G., Brockhaus M., Gentz R., Lesslauer W.
Immunogenetics 34:338-340(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Spleen.
[5]"Nucleotide sequence of the TNF type I receptor from a mouse endothelioma cell line."
Bebo B.F., Linthicum D.S.
Immunogenetics 39:450-451(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[6]"Genomic organization and promoter function of the murine tumor necrosis factor receptor beta gene."
Rothe J., Bluethmann H., Gentz R., Lesslauer W., Steinmetz M.
Mol. Immunol. 30:165-175(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C3H/He.
Tissue: Mesenchymal cell.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M60468 mRNA. Translation: AAA39751.1.
M59377 mRNA. Translation: AAA40464.1.
X59238 mRNA. Translation: CAA41922.1.
X57796 mRNA. Translation: CAA40936.1.
L26349 mRNA. Translation: AAA59361.1.
M76656, M88067, M76655 Genomic DNA. Translation: AAA40465.1.
BC004599 mRNA. Translation: AAH04599.1.
BC052675 mRNA. Translation: AAH52675.1.
PIRGQMST1. A38634.
RefSeqNP_035739.2. NM_011609.4.
UniGeneMm.1258.
Mm.474976.

3D structure databases

ProteinModelPortalP25118.
SMRP25118. Positions 41-197, 357-441.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204249. 12 interactions.
DIPDIP-34532N.
IntActP25118. 22 interactions.
MINTMINT-2631249.

PTM databases

PhosphoSiteP25118.

Proteomic databases

PaxDbP25118.
PRIDEP25118.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000032491; ENSMUSP00000032491; ENSMUSG00000030341.
GeneID21937.
KEGGmmu:21937.
UCSCuc009dul.2. mouse.

Organism-specific databases

CTD7132.
MGIMGI:1314884. Tnfrsf1a.

Phylogenomic databases

eggNOGNOG39168.
HOGENOMHOG000133001.
HOVERGENHBG058842.
InParanoidP25118.
KOK03158.
OMACLREAHY.
PhylomeDBP25118.
TreeFamTF333916.

Gene expression databases

ArrayExpressP25118.
BgeeP25118.
GenevestigatorP25118.

Family and domain databases

Gene3D1.10.533.10. 1 hit.
InterProIPR011029. DEATH-like_dom.
IPR000488. Death_domain.
IPR001368. TNFR/NGFR_Cys_rich_reg.
IPR020419. TNFR_1A.
[Graphical view]
PfamPF00531. Death. 1 hit.
PF00020. TNFR_c6. 3 hits.
[Graphical view]
PRINTSPR01918. TNFACTORR1A.
SMARTSM00005. DEATH. 1 hit.
SM00208. TNFR. 4 hits.
[Graphical view]
SUPFAMSSF47986. SSF47986. 1 hit.
PROSITEPS50017. DEATH_DOMAIN. 1 hit.
PS00652. TNFR_NGFR_1. 3 hits.
PS50050. TNFR_NGFR_2. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio301546.
PROP25118.
SOURCESearch...

Entry information

Entry nameTNR1A_MOUSE
AccessionPrimary (citable) accession number: P25118
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: April 16, 2014
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot