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Protein

Acetylcholine receptor subunit alpha

Gene

Chrna1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-629594. Highly calcium permeable postsynaptic nicotinic acetylcholine receptors.
R-RNO-629597. Highly calcium permeable nicotinic acetylcholine receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetylcholine receptor subunit alpha
Gene namesi
Name:Chrna1
Synonyms:Acra
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi69277. Chrna1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini21 – 230210ExtracellularSequence analysisAdd
BLAST
Transmembranei231 – 25525HelicalSequence analysisAdd
BLAST
Transmembranei263 – 28119HelicalSequence analysisAdd
BLAST
Transmembranei297 – 31620HelicalSequence analysisAdd
BLAST
Topological domaini317 – 428112CytoplasmicSequence analysisAdd
BLAST
Transmembranei429 – 44719HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020By similarityAdd
BLAST
Chaini21 – 457437Acetylcholine receptor subunit alphaPRO_0000000307Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi148 ↔ 162By similarity
Glycosylationi161 – 1611N-linked (GlcNAc...)Sequence analysis
Disulfide bondi212 ↔ 213Associated with receptor activationBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP25108.

PTM databases

PhosphoSiteiP25108.

Expressioni

Gene expression databases

GenevisibleiP25108. RN.

Interactioni

Subunit structurei

Pentamer of two alpha chains, and one each of the beta, delta, and gamma (in immature muscle) or epsilon (in mature muscle) chains.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024706.

Chemistry

BindingDBiP25108.

Structurei

3D structure databases

ProteinModelPortaliP25108.
SMRiP25108. Positions 21-335.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3645. Eukaryota.
ENOG410XQGR. LUCA.
GeneTreeiENSGT00760000118930.
HOGENOMiHOG000006756.
HOVERGENiHBG003756.
InParanoidiP25108.
KOiK04803.
OMAiRANKHVS.
OrthoDBiEOG72JWGV.
PhylomeDBiP25108.
TreeFamiTF315605.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25108-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELTAVLLLL GLCSAGTVLG SEHETRLVAK LFKDYSSVVR PVGDHREIVQ
60 70 80 90 100
VTVGLQLIQL INVDEVNQIV TTNVRLKQQW VDYNLKWNPD DYGGVKKIHI
110 120 130 140 150
PSEKIWRPDV VLYNNADGDF AIVKFTKVLL DYTGHITWTP PAIFKSYCEI
160 170 180 190 200
IVTHFPFDEQ NCSMKLGTWT YDGSVVAINP ESDQPDLSNF MESGEWVIKE
210 220 230 240 250
ARGWKHWVFY SCCPNTPYLD ITYHFVMQRL PLYFIVNVII PCLLFSFLTS
260 270 280 290 300
LVFYLPTDSG EKMTLSISVL LSLTVFLLVI VELIPSTSSA VPLIGKYMLF
310 320 330 340 350
TMVFVIASII ITVIVINTHH RSPSTHIMPE WVRKVFIDTI PNIMFFSTMK
360 370 380 390 400
RPSRDKQEKR IFTEDIDISD ISGKPGPPPM GFHSPLIKHP EVKSAIEGVK
410 420 430 440 450
YIAETMKSDQ ESNNASEEWK YVAMVMDHIL LGVFMLVCLI GTLAVFAGRL

IELHQQG
Length:457
Mass (Da):51,867
Last modified:May 1, 1992 - v1
Checksum:i776AE3B8DF8F68F3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74832 mRNA. Translation: CAA52826.1.
PIRiS13872.
RefSeqiNP_077811.1. NM_024485.1.
UniGeneiRn.44633.

Genome annotation databases

EnsembliENSRNOT00000024706; ENSRNOP00000024706; ENSRNOG00000018286.
GeneIDi79557.
KEGGirno:79557.
UCSCiRGD:69277. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74832 mRNA. Translation: CAA52826.1.
PIRiS13872.
RefSeqiNP_077811.1. NM_024485.1.
UniGeneiRn.44633.

3D structure databases

ProteinModelPortaliP25108.
SMRiP25108. Positions 21-335.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024706.

Chemistry

BindingDBiP25108.

PTM databases

PhosphoSiteiP25108.

Proteomic databases

PaxDbiP25108.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000024706; ENSRNOP00000024706; ENSRNOG00000018286.
GeneIDi79557.
KEGGirno:79557.
UCSCiRGD:69277. rat.

Organism-specific databases

CTDi1134.
RGDi69277. Chrna1.

Phylogenomic databases

eggNOGiKOG3645. Eukaryota.
ENOG410XQGR. LUCA.
GeneTreeiENSGT00760000118930.
HOGENOMiHOG000006756.
HOVERGENiHBG003756.
InParanoidiP25108.
KOiK04803.
OMAiRANKHVS.
OrthoDBiEOG72JWGV.
PhylomeDBiP25108.
TreeFamiTF315605.

Enzyme and pathway databases

ReactomeiR-RNO-629594. Highly calcium permeable postsynaptic nicotinic acetylcholine receptors.
R-RNO-629597. Highly calcium permeable nicotinic acetylcholine receptors.

Miscellaneous databases

PROiP25108.

Gene expression databases

GenevisibleiP25108. RN.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Primary structure and functional expression of the alpha-, beta-, gamma-, delta- and epsilon-subunits of the acetylcholine receptor from rat muscle."
    Witzemann V., Stein E., Barg B., Konno T., Koenen M., Kues W., Criado M., Hofmann M., Sakmann B.
    Eur. J. Biochem. 194:437-448(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Muscle.

Entry informationi

Entry nameiACHA_RAT
AccessioniPrimary (citable) accession number: P25108
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: June 8, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.