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P25105 (PTAFR_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 144. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (7) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Platelet-activating factor receptor

Short name=PAF-R
Short name=PAFr
Gene names
Name:PTAFR
Synonyms:PAFR
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length342 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for platelet activating factor, a chemotactic phospholipid mediator that possesses potent inflammatory, smooth-muscle contractile and hypotensive activity. Seems to mediate its action via a G protein that activates a phosphatidylinositol-calcium second messenger system. Ref.1 Ref.2 Ref.3 Ref.5

Subunit structure

Interacts with ARRB1. Ref.13

Subcellular location

Cell membrane; Multi-pass membrane protein Ref.3.

Tissue specificity

Expressed in the placenta, lung, left and right heart ventricles, heart atrium, leukocytes and differentiated HL-60 granulocytes. Ref.1 Ref.2 Ref.5

Induction

By CSF2/GM-CSF, IL5/interleukin-5 and n-butyrate. Ref.2

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
   Biological processChemotaxis
   Cellular componentCell membrane
Membrane
   Coding sequence diversityPolymorphism
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled receptor signaling pathway

Inferred from mutant phenotype Ref.2. Source: UniProtKB

chemotaxis

Traceable author statement PubMed 10601267. Source: ProtInc

cytokine production

Inferred from electronic annotation. Source: Ensembl

cytokine-mediated signaling pathway

Traceable author statement. Source: Reactome

immune response

Traceable author statement PubMed 8892648. Source: ProtInc

inflammatory response

Traceable author statement PubMed 8978777. Source: ProtInc

inositol trisphosphate biosynthetic process

Inferred from electronic annotation. Source: Ensembl

interferon-gamma-mediated signaling pathway

Traceable author statement. Source: Reactome

phosphatidylinositol-mediated signaling

Inferred from direct assay Ref.2. Source: UniProtKB

   Cellular_componentintegral component of membrane

Non-traceable author statement Ref.5Ref.1. Source: UniProtKB

integral component of plasma membrane

Traceable author statement PubMed 8168510. Source: ProtInc

membrane

Inferred from direct assay Ref.3. Source: UniProtKB

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functionG-protein coupled receptor activity

Traceable author statement Ref.4. Source: ProtInc

lipopolysaccharide binding

Inferred from electronic annotation. Source: Ensembl

lipopolysaccharide receptor activity

Inferred from electronic annotation. Source: Ensembl

phospholipid binding

Inferred from direct assay Ref.1Ref.2. Source: UniProtKB

platelet activating factor receptor activity

Inferred from direct assay Ref.5Ref.1Ref.2. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 342342Platelet-activating factor receptor
PRO_0000070092

Regions

Topological domain1 – 1616Extracellular Potential
Transmembrane17 – 3822Helical; Name=1; Potential
Topological domain39 – 5416Cytoplasmic Potential
Transmembrane55 – 7420Helical; Name=2; Potential
Topological domain75 – 9117Extracellular Potential
Transmembrane92 – 11322Helical; Name=3; Potential
Topological domain114 – 13320Cytoplasmic Potential
Transmembrane134 – 15522Helical; Name=4; Potential
Topological domain156 – 18429Extracellular Potential
Transmembrane185 – 20521Helical; Name=5; Potential
Topological domain206 – 23328Cytoplasmic Potential
Transmembrane234 – 25421Helical; Name=6; Potential
Topological domain255 – 27622Extracellular Potential
Transmembrane277 – 29620Helical; Name=7; Potential
Topological domain297 – 34246Cytoplasmic Potential

Amino acid modifications

Glycosylation1691N-linked (GlcNAc...) Potential
Disulfide bond90 ↔ 173 By similarity

Natural variations

Natural variant2241A → D. Ref.14
Corresponds to variant rs5938 [ dbSNP | Ensembl ].
VAR_011851
Natural variant3381N → S. Ref.14
Corresponds to variant rs5939 [ dbSNP | Ensembl ].
VAR_011852

Experimental info

Sequence conflict281L → P in AAA60108. Ref.6
Sequence conflict661F → L in AAA60108. Ref.6
Sequence conflict951C → R in AAA60108. Ref.6
Sequence conflict227 – 2282KR → TG in AAA60214. Ref.4
Sequence conflict227 – 2282KR → TT in AAA60108. Ref.6
Sequence conflict2471P → A in AAA60108. Ref.6
Sequence conflict3161K → N in AAB24695. Ref.5

Sequences

Sequence LengthMass (Da)Tools
P25105 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: 890073C9EBA79228

FASTA34239,203
        10         20         30         40         50         60 
MEPHDSSHMD SEFRYTLFPI VYSIIFVLGV IANGYVLWVF ARLYPCKKFN EIKIFMVNLT 

        70         80         90        100        110        120 
MADMLFLITL PLWIVYYQNQ GNWILPKFLC NVAGCLFFIN TYCSVAFLGV ITYNRFQAVT 

       130        140        150        160        170        180 
RPIKTAQANT RKRGISLSLV IWVAIVGAAS YFLILDSTNT VPDSAGSGNV TRCFEHYEKG 

       190        200        210        220        230        240 
SVPVLIIHIF IVFSFFLVFL IILFCNLVII RTLLMQPVQQ QRNAEVKRRA LWMVCTVLAV 

       250        260        270        280        290        300 
FIICFVPHHV VQLPWTLAEL GFQDSKFHQA INDAHQVTLC LLSTNCVLDP VIYCFLTKKF 

       310        320        330        340 
RKHLTEKFYS MRSSRKCSRA TTDTVTEVVV PFNQIPGNSL KN 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of a human cDNA that encodes a functional receptor for platelet activating factor."
Ye R.D., Prossnitz E.R., Zou A., Cochrane C.G.
Biochem. Biophys. Res. Commun. 180:105-111(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
Tissue: Granulocyte.
[2]"Molecular cloning and expression of platelet-activating factor receptor from human leukocytes."
Nakamura M., Honda Z., Izumi T., Sakanaka C., Mutoh H., Minami M., Bito H., Seyama Y., Matsumoto T., Noma M., Shimizu T.
J. Biol. Chem. 266:20400-20405(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION.
Tissue: Leukocyte.
[3]"The human leukocyte platelet-activating factor receptor. cDNA cloning, cell surface expression, and construction of a novel epitope-bearing analog."
Kunz D., Gerard N.P., Gerard C.
J. Biol. Chem. 267:9101-9106(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
Tissue: Leukocyte.
[4]"The human platelet-activating factor receptor gene (PTAFR) contains no introns and maps to chromosome 1."
Seyfried C.E., Schweickart V.L., Godiska R., Gray P.W.
Genomics 13:832-834(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Fetal liver.
[5]"Molecular cloning and characterization of the platelet-activating factor receptor gene expressed in the human heart."
Sugimoto T., Tsuchimochi H., McGregor C.G.A., Mutoh H., Shimizu T., Kurachi Y.
Biochem. Biophys. Res. Commun. 189:617-624(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
Tissue: Heart ventricle.
[6]"Nucleotide sequence of platelet-activating-factor receptor derived from HeLa cell genomic DNA and Rhesus monkey genomic DNA."
Behal R.H., Debuysere M.S., Olson M.S.
Submitted (DEC-1992) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Cervix carcinoma.
[7]"Cloning of a human platelet-activating factor receptor gene: evidence for an intron in the 5'-untranslated region."
Chase P.B., Halonen M., Regan J.W.
Am. J. Respir. Cell Mol. Biol. 8:240-244(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[8]"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
Warren C.N., Aronstam R.S., Sharma S.V.
Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Placenta.
[9]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[10]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Umbilical cord blood.
[11]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[12]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain and Lymph.
[13]"Platelet-activating factor-induced clathrin-mediated endocytosis requires beta-arrestin-1 recruitment and activation of the p38 MAPK signalosome at the plasma membrane for actin bundle formation."
McLaughlin N.J., Banerjee A., Kelher M.R., Gamboni-Robertson F., Hamiel C., Sheppard F.R., Moore E.E., Silliman C.C.
J. Immunol. 176:7039-7050(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ARRB1.
[14]"Characterization of single-nucleotide polymorphisms in coding regions of human genes."
Cargill M., Altshuler D., Ireland J., Sklar P., Ardlie K., Patil N., Shaw N., Lane C.R., Lim E.P., Kalyanaraman N., Nemesh J., Ziaugra L., Friedland L., Rolfe A., Warrington J., Lipshutz R., Daley G.Q., Lander E.S.
Nat. Genet. 22:231-238(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS ASP-224 AND SER-338.
[15]Erratum
Cargill M., Altshuler D., Ireland J., Sklar P., Ardlie K., Patil N., Shaw N., Lane C.R., Lim E.P., Kalyanaraman N., Nemesh J., Ziaugra L., Friedland L., Rolfe A., Warrington J., Lipshutz R., Daley G.Q., Lander E.S.
Nat. Genet. 23:373-373(1999)
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M80436 mRNA. Translation: AAA60001.1.
D10202 mRNA. Translation: BAA01050.1.
M76674 mRNA. Translation: AAA60002.1.
M88177 Genomic DNA. Translation: AAA60214.1.
S52624 mRNA. Translation: AAB24695.2.
L07334 mRNA. Translation: AAA60108.1.
S56396 Genomic DNA. Translation: AAB25755.1.
AY275466 mRNA. Translation: AAP32298.1.
BT009801 mRNA. Translation: AAP88803.1.
AK290240 mRNA. Translation: BAF82929.1.
CH471059 Genomic DNA. Translation: EAX07711.1.
BC013816 mRNA. Translation: AAH13816.1.
BC063000 mRNA. Translation: AAH63000.1.
CCDSCCDS318.1.
PIRA40191.
RefSeqNP_000943.1. NM_000952.4.
NP_001158193.1. NM_001164721.1.
NP_001158194.1. NM_001164722.2.
NP_001158195.1. NM_001164723.2.
UniGeneHs.77542.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2B0Xmodel-A1-342[»]
ProteinModelPortalP25105.
SMRP25105. Positions 6-304.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid111696. 12 interactions.
IntActP25105. 4 interactions.
STRING9606.ENSP00000301974.

Chemistry

BindingDBP25105.
ChEMBLCHEMBL250.
GuidetoPHARMACOLOGY334.

Protein family/group databases

TCDB9.A.14.13.3. the g-protein-coupled receptor (gpcr) family.
GPCRDBSearch...

PTM databases

PhosphoSiteP25105.

Polymorphism databases

DMDM129557.

Proteomic databases

PRIDEP25105.

Protocols and materials databases

DNASU5724.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000305392; ENSP00000301974; ENSG00000169403.
ENST00000373857; ENSP00000362965; ENSG00000169403.
ENST00000539896; ENSP00000442658; ENSG00000169403.
GeneID5724.
KEGGhsa:5724.
UCSCuc001bpl.3. human.

Organism-specific databases

CTD5724.
GeneCardsGC01M028473.
HGNCHGNC:9582. PTAFR.
HPAHPA027543.
MIM173393. gene.
neXtProtNX_P25105.
PharmGKBPA33933.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG149133.
HOGENOMHOG000013041.
HOVERGENHBG101106.
InParanoidP25105.
KOK04279.
OMAFLCNLAG.
OrthoDBEOG7QVM3C.
PhylomeDBP25105.
TreeFamTF350009.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.
REACT_6900. Immune System.

Gene expression databases

BgeeP25105.
CleanExHS_PTAFR.
GenevestigatorP25105.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR002282. PAF_rcpt.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PR01153. PAFRECEPTOR.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiPlatelet-activating_factor_receptor.
GenomeRNAi5724.
NextBio22248.
PROP25105.
SOURCESearch...

Entry information

Entry namePTAFR_HUMAN
AccessionPrimary (citable) accession number: P25105
Secondary accession number(s): A3KMC8, A8K2H5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: July 9, 2014
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries