Reviewed,
UniProtKB/Swiss-Prot P25103 (NK1R_HUMAN)
Last modified
January 19, 2010.
Version 108.
History...
Clusters with 100%,
90%,
50% identity |
Documents (6) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Substance-P receptor Short name=SPR Alternative name(s): NK-1 receptor Short name=NK-1R Tachykinin receptor 1 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 407 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | This is a receptor for the tachykinin neuropeptide substance P. It is probably associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinity of this receptor to tachykinins is: substance P > substance K > neuromedin-K. |
| Subunit structure | Interacts with ARRB1 By similarity. |
| Subcellular location | |
| Sequence similarities | Belongs to the G-protein coupled receptor 1 family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cell membrane Membrane |
| Coding sequence diversity | Polymorphism |
| Domain | Transmembrane |
| Molecular function | G-protein coupled receptor Receptor Transducer |
| PTM | Disulfide bond Glycoprotein Lipoprotein Palmitate Phosphoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | activation of phospholipase C activity by G-protein coupled receptor protein signaling pathway coupled to IP3 second messenger Ref.3 Traceable author statement. Source: ProtInc detection of abiotic stimulusTraceable author statement. Source: ProtInc mechanosensory behaviorTraceable author statement. Source: ProtInc tachykinin receptor signaling pathwayInferred from direct assay. Source: UniProtKB |
| Cellular component | integral to plasma membrane Ref.3 Traceable author statement. Source: ProtInc |
| Molecular function | protein binding Inferred from physical interaction. Source: UniProtKB |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 407 | 407 | Substance-P receptor | PRO_0000069885 | |||||||
Regions | |||||||||||
| Topological domain | 1 – 31 | 31 | Extracellular Potential | ||||||||
| Transmembrane | 32 – 54 | 23 | 1 Potential | ||||||||
| Topological domain | 55 – 64 | 10 | Cytoplasmic Potential | ||||||||
| Transmembrane | 65 – 86 | 22 | 2 Potential | ||||||||
| Topological domain | 87 – 106 | 20 | Extracellular Potential | ||||||||
| Transmembrane | 107 – 128 | 22 | 3 Potential | ||||||||
| Topological domain | 129 – 148 | 20 | Cytoplasmic Potential | ||||||||
| Transmembrane | 149 – 169 | 21 | 4 Potential | ||||||||
| Topological domain | 170 – 194 | 25 | Extracellular Potential | ||||||||
| Transmembrane | 195 – 219 | 25 | 5 Potential | ||||||||
| Topological domain | 220 – 248 | 29 | Cytoplasmic Potential | ||||||||
| Transmembrane | 249 – 270 | 22 | 6 Potential | ||||||||
| Topological domain | 271 – 283 | 13 | Extracellular Potential | ||||||||
| Transmembrane | 284 – 308 | 25 | 7 Potential | ||||||||
| Topological domain | 309 – 407 | 99 | Cytoplasmic Potential | ||||||||
Sites | |||||||||||
| Binding site | 197 | 1 | Antagonist CP 96345 | ||||||||
Amino acid modifications | |||||||||||
| Lipidation | 322 | 1 | S-palmitoyl cysteine Potential | ||||||||
| Glycosylation | 14 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 18 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Disulfide bond | 105 ↔ 180 | By similarity | |||||||||
Natural variations | |||||||||||
| Natural variant | 192 | 1 | Y → H Display properties similar to those of the wild-type receptor. Ref.12 | VAR_026826 | |||||||
Experimental info | |||||||||||
| Sequence conflict | 116 | 1 | V → C in AAA59936. Ref.3 | ||||||||
| Sequence conflict | 218 | 1 | V → I in AAA59936. Ref.3 | ||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Molecular cloning, structural characterization and functional expression of the human substance P receptor." Takeda Y., Chou K.B., Takeda J., Sachais B.S., Krause J.E. Biochem. Biophys. Res. Commun. 179:1232-1240(1991) [PubMed: 1718267] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "Isolation and characterisation of the human lung NK-1 receptor cDNA." Hopkins B., Powell S.J., Danks P., Briggs I., Graham A. Biochem. Biophys. Res. Commun. 180:1110-1117(1991) [PubMed: 1659396] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lung. |
| [3] | "Human substance P receptor (NK-1): organization of the gene, chromosome localization, and functional expression of cDNA clones." Gerard N.P., Garraway L.A., Eddy R.L. Jr., Shows T.B., Iijima H., Paquet J.L., Gerard C. Biochemistry 30:10640-10646(1991) [PubMed: 1657150] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [4] | "The primary structure and gene organization of human substance P and neuromedin K receptors." Takahashi K., Tanaka A., Hara M., Nakanishi S. Eur. J. Biochem. 204:1025-1033(1992) [PubMed: 1312928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Placenta. |
| [5] | "Differential activation of intracellular effector by two isoforms of human neurokinin-1 receptor." Fong T.M., Anderson S.A., Yu H., Huang R.-R.C., Strader C.D. Mol. Pharmacol. 41:24-30(1992) [PubMed: 1310144] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [6] | "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Kopatz S.A., Aronstam R.S., Sharma S.V. Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain. |
| [7] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain. |
| [8] | Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [9] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [10] | "The human tachykinin NK1 (short form) and tachykinin NK4 receptor: a reappraisal." Page N.M., Bell N.J. Eur. J. Pharmacol. 437:27-30(2002) [PubMed: 11864635] [Abstract] Cited for: SHOWS THAT THERE IS NO SHORT SPLICED ISOFORM. |
| [11] | "Amino-aromatic interaction between histidine 197 of the neurokinin-1 receptor and CP 96345." Fong T.M., Cascieri M.A., Yu H., Bansai A., Swain C., Strader C.D. Nature 362:350-353(1993) [PubMed: 8384323] [Abstract] Cited for: BINDING TO ANTAGONIST CP 96345. |
| [12] | "Identification of single-nucleotide polymorphisms of the human neurokinin 1 receptor gene and pharmacological characterization of a Y192H variant." Randolph G.P., Simon J.S., Arreaza M.G., Qiu P., Lachowicz J.E., Duffy R.A. Pharmacogenomics J. 4:394-402(2004) [PubMed: 15452552] [Abstract] Cited for: VARIANT HIS-192, CHARACTERIZATION OF VARIANT HIS-192. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | S62045 mRNA. Translation: AAB20168.2. M74290 mRNA. Translation: AAA60601.1. M81797 mRNA. Translation: AAA59933.1. M76675 mRNA. Translation: AAA59936.1. X65177 X65181 Genomic DNA. Translation: CAA46292.1. M84425 mRNA. Translation: AAA36641.1. M84426 mRNA. Translation: AAA36644.1. AY462098 mRNA. Translation: AAR23925.1. AK289765 mRNA. Translation: BAF82454.1. CH471053 Genomic DNA. Translation: EAW99596.1. BC074911 mRNA. Translation: AAH74911.1. BC074912 mRNA. Translation: AAH74912.1. |
| IPI | IPI00012729. |
| PIR | JQ1274. A41134. |
| RefSeq | NP_001049.1. NP_056542.1. |
| UniGene | Hs.633301 |
3D structure databases | |
| SMR | P25103. Positions 22-324. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | P25103. |
Protein family/group databases | |
| GPCRDB | Search... |
Proteomic databases | |
| PRIDE | P25103. |
Genome annotation databases | |
| Ensembl | ENST00000305249; ENSP00000303522; ENSG00000115353; Homo sapiens. [Genome view] |
| GeneID | 6869. |
| KEGG | hsa:6869. |
| UCSC | uc002sng.1. human. |
Organism-specific databases | |
| CTD | 6869. |
| GeneCards | GC02M075187. |
| H-InvDB | HIX0029906. |
| HGNC | HGNC:11526. TACR1. |
| MIM | 162323. gene. |
| PharmGKB | PA36302. |
| GenAtlas | Search... |
Phylogenomic databases | |
| eggNOG | prNOG17440. |
| HOGENOM | HBG443644. |
| HOVERGEN | P25103. |
| InParanoid | P25103. |
| OMA | PVDSDLF. |
| OrthoDB | EOG9W6RFC. |
| PhylomeDB | P25103. |
Enzyme and pathway databases | |
| Reactome | REACT_14797. Signaling by GPCR. |
Gene expression databases | |
| ArrayExpress | P25103. |
| Bgee | P25103. |
| CleanEx | HS_TACR1. |
| Genevestigator | P25103. |
| GermOnline | ENSG00000115353. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR000276. 7TM_GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. IPR001681. Neurokn_rcpt. IPR000046. NK1_rcpt. [Graphical view] |
| Pfam | PF00001. 7tm_1. 1 hit. [Graphical view] |
| PRINTS | PR00237. GPCRRHODOPSN. PR01024. NEUROKININ1R. PR00244. NEUROKININR. |
| PROSITE | PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| DrugBank | DB00673. Aprepitant. DB01221. Ketamine. DB04894. Vapreotide. |
| NextBio | 26811. |
| SOURCE | Search... |
Entry information
| Entry name | NK1R_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P25103 Secondary accession number(s): A8K150 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| 7-transmembrane G-linked receptors List of 7-transmembrane G-linked receptor entries |
| Human chromosome 2 Human chromosome 2: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


