P25099 (AA1R_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenosine receptor A1

Gene names

Name:

Adora1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Widely expressed in brain and spinal cord.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	activation of MAPKK activity Inferred from direct assay PubMed 12764156. Source: RGD adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Inferred from mutant phenotype PubMed 16286581. Source: RGD cognition Inferred from mutant phenotype PubMed 19307960. Source: RGD detection of temperature stimulus involved in sensory perception of pain Inferred from mutant phenotype PubMed 20065990. Source: RGD lipid catabolic process Inferred from mutant phenotype PubMed 19093734. Source: RGD negative regulation of acute inflammatory response Inferred from direct assay PubMed 2167912. Source: RGD negative regulation of apoptotic process Inferred from direct assay PubMed 12578815. Source: RGD negative regulation of blood pressure Inferred from mutant phenotype PubMed 16690710. Source: RGD negative regulation of cardiac muscle contraction Inferred from mutant phenotype PubMed 19525381. Source: RGD negative regulation of cell proliferation Inferred from direct assay PubMed 12021208. Source: RGD negative regulation of circadian sleep/wake cycle, non-REM sleep Inferred from mutant phenotype PubMed 19491711. Source: RGD negative regulation of glutamate secretion Inferred from direct assay PubMed 16481441. Source: RGD negative regulation of heart contraction Inferred from mutant phenotype PubMed 16286581. Source: RGD negative regulation of hormone secretion Inferred from mutant phenotype PubMed 15044554. Source: RGD negative regulation of leukocyte migration Inferred from electronic annotation. Source: Compara negative regulation of lipid catabolic process Inferred from direct assay PubMed 15220221. Source: RGD negative regulation of mucus secretion Inferred from electronic annotation. Source: Compara negative regulation of neurotrophin production Inferred from mutant phenotype PubMed 19524026. Source: RGD negative regulation of renal sodium excretion Inferred from mutant phenotype PubMed 14993484. Source: RGD negative regulation of synaptic transmission, GABAergic Inferred from mutant phenotype PubMed 12626609. Source: RGD negative regulation of synaptic transmission, glutamatergic Inferred from direct assay PubMed 16481441. Source: RGD negative regulation of vasodilation Inferred from direct assay PubMed 12100087. Source: RGD positive regulation of blood pressure Inferred from direct assay PubMed 12234813. Source: RGD positive regulation of epidermal growth factor-activated receptor activity Inferred from mutant phenotype PubMed 19574994. Source: RGD positive regulation of nucleoside transport Inferred from direct assay PubMed 15024061. Source: RGD positive regulation of peptide secretion Inferred from mutant phenotype PubMed 16286581. Source: RGD positive regulation of potassium ion transport Inferred from direct assay PubMed 9261808. Source: RGD positive regulation of protein dephosphorylation Inferred from direct assay PubMed 12234781. Source: RGD protein targeting to membrane Inferred from mutant phenotype PubMed 19879903. Source: RGD regulation of excitatory postsynaptic membrane potential Inferred from mutant phenotype PubMed 19942300. Source: RGD regulation of glomerular filtration Inferred from electronic annotation. Source: Compara regulation of respiratory gaseous exchange by neurological system process Inferred from mutant phenotype PubMed 16352472. Source: RGD regulation of synaptic plasticity Inferred from mutant phenotype PubMed 14596853. Source: RGD relaxation of vascular smooth muscle Inferred from mutant phenotype PubMed 19443947. Source: RGD response to hypoxia Inferred from mutant phenotype PubMed 16690710. Source: RGD temperature homeostasis Inferred from mutant phenotype PubMed 16352472. Source: RGD
Cellular_component	asymmetric synapse Inferred from direct assay PubMed 16481441. Source: RGD axolemma Inferred from direct assay PubMed 16481441. Source: RGD basolateral plasma membrane Inferred from direct assay PubMed 15024061. Source: RGD endomembrane system Inferred from direct assay PubMed 16952160. Source: RGD endoplasmic reticulum Inferred from direct assay PubMed 12764156. Source: RGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW neuronal cell body Inferred from direct assay PubMed 15044554. Source: RGD postsynaptic density Inferred from direct assay PubMed 14499945. Source: RGD postsynaptic membrane Inferred from direct assay PubMed 16481441. Source: RGD presynaptic active zone Inferred from direct assay PubMed 16481441. Source: RGD presynaptic membrane Inferred from direct assay PubMed 16481441. Source: RGD terminal bouton Inferred from direct assay PubMed 16952160. Source: RGD
Molecular_function	G-protein beta/gamma-subunit complex binding Inferred from direct assay PubMed 12764156. Source: RGD G-protein coupled adenosine receptor activity Inferred from direct assay PubMed 9261808. Source: RGD heterotrimeric G-protein binding Inferred from direct assay PubMed 12764156. Source: RGD phospholipase C activity Inferred from mutant phenotype PubMed 19525381. Source: RGD purine nucleoside binding Inferred from physical interaction PubMed 11906959. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	Adenosine receptor A1		PRO_0000068994
Regions
	Topological domain	1 – 10	10	Extracellular Potential
	Transmembrane	11 – 33	23	Helical; Name=1; Potential
	Topological domain	34 – 46	13	Cytoplasmic Potential
	Transmembrane	47 – 69	23	Helical; Name=2; Potential
	Topological domain	70 – 80	11	Extracellular Potential
	Transmembrane	81 – 102	22	Helical; Name=3; Potential
	Topological domain	103 – 123	21	Cytoplasmic Potential
	Transmembrane	124 – 146	23	Helical; Name=4; Potential
	Topological domain	147 – 176	30	Extracellular Potential
	Transmembrane	177 – 201	25	Helical; Name=5; Potential
	Topological domain	202 – 235	34	Cytoplasmic Potential
	Transmembrane	236 – 259	24	Helical; Name=6; Potential
	Topological domain	260 – 267	8	Extracellular Potential
	Transmembrane	268 – 292	25	Helical; Name=7; Potential
	Topological domain	293 – 326	34	Cytoplasmic Potential
Amino acid modifications
	Lipidation	309	1	S-palmitoyl cysteine Potential
	Glycosylation	148	1	N-linked (GlcNAc...) Potential
	Glycosylation	159	1	N-linked (GlcNAc...) Potential
	Disulfide bond	80 ↔ 169		By similarity
Experimental info
	Sequence conflict	229 – 230	2	EL → DV Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P25099 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: E43785CA993CFF15	FASTA	326	36,695
	10 20 30 40 50 60 MPPYISAFQA AYIGIEVLIA LVSVPGNVLV IWAVKVNQAL RDATFCFIVS LAVADVAVGA 70 80 90 100 110 120 LVIPLAILIN IGPQTYFHTC LMVACPVLIL TQSSILALLA IAVDRYLRVK IPLRYKTVVT 130 140 150 160 170 180 QRRAAVAIAG CWILSLVVGL TPMFGWNNLS VVEQDWRANG SVGEPVIKCE FEKVISMEYM 190 200 210 220 230 240 VYFNFFVWVL PPLLLMVLIY LEVFYLIRKQ LNKKVSASSG DPQKYYGKEL KIAKSLALIL 250 260 270 280 290 300 FLFALSWLPL HILNCITLFC PTCQKPSILI YIAIFLTHGN SAMNPIVYAF RIHKFRVTFL 310 320 KIWNDHFRCQ PKPPIDEDLP EEKAED

References

[1]	"Molecular cloning and characterization of a rat A1-adenosine receptor that is widely expressed in brain and spinal cord." Reppert S.M., Weaver D.R., Stehle J.H., Rivkees S.A. Mol. Endocrinol. 5:1037-1048(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning and expression of an A1 adenosine receptor from rat brain." Mahan L.C., McVittie L.D., Smyk-Randall E.M., Nakata H., Monsma F.J. Jr., Gerfen C.R., Sibley D.R. Mol. Pharmacol. 40:1-7(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[3]	Hirabatake Y., Takao K., Hagiwara S., Kasanuki H., Hosoda S., Kokubun S. Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Heart.
[4]	Yip L., Mourelatos K., Hewitt J., Kwok Y.N. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M69045 mRNA. Translation: AAB07231.1. M64299 mRNA. Translation: AAA74471.1. AB001089 mRNA. Translation: BAA19231.1. AF042079 mRNA. Translation: AAC03772.1.
IPI	IPI00327968.
PIR	A40376.
RefSeq	NP_058851.2. NM_017155.2.
UniGene	Rn.32078.
3D structure databases
ProteinModelPortal	P25099.
ModBase	Search...
Protein-protein interaction databases
IntAct	P25099. 1 interaction.
MINT	MINT-242252.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P25099.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000004602; ENSRNOP00000004602; ENSRNOG00000003442.
GeneID	29290.
KEGG	rno:29290.
UCSC	RGD:2048. rat.
Organism-specific databases
CTD	134.
RGD	2048. Adora1.
Phylogenomic databases
eggNOG	NOG284456.
GeneTree	ENSGT00550000074524.
HOGENOM	HOG000015770.
HOVERGEN	HBG106962.
InParanoid	P25099.
KO	K04265.
OMA	IPLRYKM.
OrthoDB	EOG4BVRV7.
Gene expression databases
Genevestigator	P25099.
GermOnline	ENSRNOG00000003442. Rattus norvegicus.
Family and domain databases
InterPro	IPR001068. Adeno_A1_rcpt. IPR001634. Adenosn_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00552. ADENOSINEA1R. PR00424. ADENOSINER. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P25099.
ChEMBL	CHEMBL318.
NextBio	608685.

Entry information

Entry name

AA1R_RAT

Accession

Primary (citable) accession number: P25099

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	November 1, 1997
Last modified:	April 3, 2013
This is version 109 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program