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P25095 (AGTRA_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 126. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-1A angiotensin II receptor
Alternative name(s):
Angiotensin II type-1A receptor
Short name=AT1A
Gene names
Name:Agtr1
Synonyms:Agtr1a, At1a
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length359 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.

Subunit structure

Interacts with MAS1 By similarity. Interacts with ARRB1. Ref.8

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Is expressed in the liver, kidney, aorta, lung, uterus, ovary, spleen, heart, adrenal gland, and vascular smooth muscle cell.

Post-translational modification

C-terminal Ser or Thr residues may be phosphorylated.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
Lipoprotein
Palmitate
Phosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled receptor signaling pathway

Inferred from mutant phenotype PubMed 12446719PubMed 9092554. Source: RGD

activation of Janus kinase activity

Inferred from mutant phenotype PubMed 9314843. Source: RGD

aging

Inferred from expression pattern PubMed 21318332. Source: RGD

blood vessel development

Inferred from electronic annotation. Source: Ensembl

brain renin-angiotensin system

Inferred from electronic annotation. Source: Ensembl

cellular response to dexamethasone stimulus

Inferred from direct assay PubMed 17537984. Source: UniProtKB

dopamine biosynthetic process

Inferred from mutant phenotype PubMed 20097214. Source: RGD

drinking behavior

Inferred from electronic annotation. Source: Ensembl

heart development

Inferred from electronic annotation. Source: Ensembl

inflammatory response

Inferred from electronic annotation. Source: Ensembl

kidney development

Inferred from electronic annotation. Source: Ensembl

negative regulation of smooth muscle cell apoptotic process

Inferred from electronic annotation. Source: Ensembl

phospholipase C-activating G-protein coupled receptor signaling pathway

Inferred from direct assay PubMed 11906170. Source: RGD

phospholipase C-activating angiotensin-activated signaling pathway

Inferred from direct assay PubMed 11906170. Source: GOC

positive regulation of blood pressure

Inferred from mutant phenotype PubMed 20702798. Source: RGD

positive regulation of branching involved in ureteric bud morphogenesis

Inferred from mutant phenotype PubMed 17537984. Source: UniProtKB

positive regulation of cell proliferation

Inferred from mutant phenotype PubMed 22645419. Source: RGD

positive regulation of cytokine secretion

Inferred from electronic annotation. Source: Ensembl

positive regulation of cytosolic calcium ion concentration

Inferred from direct assay PubMed 12110004. Source: RGD

positive regulation of receptor recycling

Inferred from direct assay PubMed 14559250. Source: UniProtKB

positive regulation of superoxide anion generation

Inferred from mutant phenotype PubMed 21076237. Source: RGD

regulation of blood pressure

Traceable author statement PubMed 15569855. Source: RGD

regulation of pH

Inferred from direct assay PubMed 11906170. Source: RGD

regulation of renal output by angiotensin

Inferred from electronic annotation. Source: Ensembl

regulation of vasoconstriction

Inferred from electronic annotation. Source: InterPro

renin secretion into blood stream

Inferred from electronic annotation. Source: Ensembl

renin-angiotensin regulation of aldosterone production

Inferred from mutant phenotype PubMed 21318332. Source: RGD

response to estrogen

Inferred from direct assay PubMed 12810582. Source: RGD

vasoconstriction

Inferred from mutant phenotype PubMed 20955746. Source: RGD

   Cellular_componentGolgi apparatus

Inferred from direct assay PubMed 14559250. Source: UniProtKB

basolateral plasma membrane

Inferred from direct assay PubMed 12110004. Source: RGD

cytoplasmic vesicle

Inferred from direct assay PubMed 14559250. Source: UniProtKB

dendrite

Inferred from direct assay PubMed 15590154. Source: RGD

endomembrane system

Inferred from direct assay PubMed 15590154. Source: RGD

integral component of membrane

Traceable author statement PubMed 11906170. Source: RGD

membrane

Inferred from direct assay PubMed 12446719. Source: RGD

organelle outer membrane

Inferred from direct assay PubMed 15590154. Source: RGD

plasma membrane

Inferred from direct assay PubMed 14559250PubMed 19023134. Source: UniProtKB

recycling endosome

Inferred from direct assay PubMed 14559250. Source: RGD

vesicle lumen

Inferred from direct assay PubMed 12110004. Source: RGD

vesicle membrane

Inferred from direct assay PubMed 12110004. Source: RGD

   Molecular_functionD1 dopamine receptor binding

Inferred from physical interaction PubMed 22193384. Source: RGD

G-protein alpha-subunit binding

Inferred from mutant phenotype PubMed 9092554. Source: RGD

angiotensin type I receptor activity

Inferred from direct assay PubMed 11906170. Source: RGD

angiotensin type II receptor activity

Inferred from electronic annotation. Source: InterPro

peptide hormone binding

Inferred from mutant phenotype PubMed 12110004PubMed 12446719PubMed 15569855. Source: RGD

protein kinase binding

Inferred from physical interaction PubMed 9287353PubMed 9314843. Source: RGD

protein self-association

Inferred from mutant phenotype PubMed 12446719. Source: RGD

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

AGTP0101910EBI-764979,EBI-751728From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 359359Type-1A angiotensin II receptor
PRO_0000069160

Regions

Topological domain1 – 2727Extracellular Potential
Transmembrane28 – 5225Helical; Name=1; Potential
Topological domain53 – 6412Cytoplasmic Potential
Transmembrane65 – 8723Helical; Name=2; Potential
Topological domain88 – 10215Extracellular Potential
Transmembrane103 – 12422Helical; Name=3; Potential
Topological domain125 – 14218Cytoplasmic Potential
Transmembrane143 – 16220Helical; Name=4; Potential
Topological domain163 – 19230Extracellular Potential
Transmembrane193 – 21422Helical; Name=5; Potential
Topological domain215 – 24026Cytoplasmic Potential
Transmembrane241 – 26222Helical; Name=6; Potential
Topological domain263 – 27513Extracellular Potential
Transmembrane276 – 29621Helical; Name=7; Potential
Topological domain297 – 35963Cytoplasmic Potential

Amino acid modifications

Lipidation3551S-palmitoyl cysteine Potential
Glycosylation41N-linked (GlcNAc...) Potential
Glycosylation1761N-linked (GlcNAc...) Potential
Glycosylation1881N-linked (GlcNAc...) Potential
Disulfide bond101 ↔ 180 By similarity

Experimental info

Sequence conflict811L → C in AAA40738. Ref.2
Sequence conflict1091S → T Ref.2
Sequence conflict1091S → T Ref.5

Sequences

Sequence LengthMass (Da)Tools
P25095 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: 200EAE56DB4DA678

FASTA35940,890
        10         20         30         40         50         60 
MALNSSAEDG IKRIQDDCPK AGRHSYIFVM IPTLYSIIFV VGIFGNSLVV IVIYFYMKLK 

        70         80         90        100        110        120 
TVASVFLLNL ALADLCFLLT LPLWAVYTAM EYRWPFGNHL CKIASASVSF NLYASVFLLT 

       130        140        150        160        170        180 
CLSIDRYLAI VHPMKSRLRR TMLVAKVTCI IIWLMAGLAS LPAVIHRNVY FIENTNITVC 

       190        200        210        220        230        240 
AFHYESRNST LPIGLGLTKN ILGFLFPFLI ILTSYTLIWK ALKKAYEIQK NKPRNDDIFR 

       250        260        270        280        290        300 
IIMAIVLFFF FSWVPHQIFT FLDVLIQLGV IHDCKISDIV DTAMPITICI AYFNNCLNPL 

       310        320        330        340        350 
FYGFLGKKFK KYFLQLLKYI PPKAKSHSSL STKMSTLSYR PSDNMSSSAK KPASCFEVE 

« Hide

References

« Hide 'large scale' references
[1]"Isolation of a cDNA encoding the vascular type-1 angiotensin II receptor."
Murphy T.J., Alexander R.W., Griendling K.K., Runge M.S., Bernstein K.E.
Nature 351:233-236(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Vascular smooth muscle.
[2]"Rat angiotensin II receptor: cDNA sequence and regulation of the gene expression."
Iwai N., Yamano Y., Chaki S., Konishi F., Bardhan S., Tibbetts C., Sasaki K., Hasegawa M., Matsuda Y., Inagami T.
Biochem. Biophys. Res. Commun. 177:299-304(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Kidney.
[3]"The genomic organization of the rat AT1 angiotensin receptor."
Langford K.G., Frenzel K., Martin B.M., Bernstein K.E.
Biochem. Biophys. Res. Commun. 183:1025-1032(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[4]"Identification of two subtypes in the rat type I angiotensin II receptor."
Iwai N., Inagami T.
FEBS Lett. 298:257-260(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Tissue: Adrenal gland.
[5]"Cloning, expression and regulation of angiotensin II receptors."
Inagami T., Iwai N., Sasaki K., Yamano Y., Bardhan S., Chaki S., Guo D.F., Furuta H., Ohyama K., Kambayashi Y., Takahashi K., Ichiki T.
Eur. Heart J. 15:104-107(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
[7]"Characterization of an angiotensin type-1 receptor partial cDNA from rat kidney: evidence for a novel AT1B receptor subtype."
Ye M.Q., Healy D.P.
Biochem. Biophys. Res. Commun. 185:204-210(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 84-259.
Tissue: Kidney.
[8]"Association of beta-Arrestin 1 with the type 1A angiotensin II receptor involves phosphorylation of the receptor carboxyl terminus and correlates with receptor internalization."
Qian H., Pipolo L., Thomas W.G.
Mol. Endocrinol. 15:1706-1719(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ARRB1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X62295 mRNA. Translation: CAA44183.1.
M74054 mRNA. Translation: AAA40738.1.
BC078810 mRNA. Translation: AAH78810.1.
M86912 Genomic DNA. No translation available.
PIRJC2134.
RefSeqNP_112247.2. NM_030985.4.
XP_006253944.1. XM_006253882.1.
UniGeneRn.9814.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2AC6model-A1-359[»]
2AH3model-A1-359[»]
ProteinModelPortalP25095.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid246370. 4 interactions.
IntActP25095. 6 interactions.
MINTMINT-1503145.
STRING10116.ENSRNOP00000034687.

Chemistry

BindingDBP25095.
ChEMBLCHEMBL329.
GuidetoPHARMACOLOGY34.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP25095.

Proteomic databases

PaxDbP25095.
PRIDEP25095.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000038532; ENSRNOP00000034687; ENSRNOG00000018346.
GeneID24180.
KEGGrno:24180.
UCSCRGD:2070. rat.

Organism-specific databases

CTD11607.
RGD2070. Agtr1a.

Phylogenomic databases

eggNOGNOG256015.
GeneTreeENSGT00720000108671.
HOGENOMHOG000234122.
HOVERGENHBG104998.
InParanoidP25095.
KOK04166.
OMACIVIWLL.
OrthoDBEOG7J4473.
PhylomeDBP25095.
TreeFamTF330024.

Gene expression databases

GenevestigatorP25095.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR000190. ATII_AT1_rcpt.
IPR000248. ATII_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00241. ANGIOTENSINR.
PR00635. ANGIOTENSN1R.
PR00237. GPCRRHODOPSN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio602525.
PROP25095.

Entry information

Entry nameAGTRA_RAT
AccessionPrimary (citable) accession number: P25095
Secondary accession number(s): Q9QVS5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: April 16, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries