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Protein

Fumarylacetoacetase

Gene

Fah

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

4-fumarylacetoacetate + H2O = acetoacetate + fumarate.By similarity

Cofactori

Protein has several cofactor binding sites:
  • Ca2+By similarity
  • Mg2+By similarity

Pathwayi: L-phenylalanine degradation

This protein is involved in step 6 of the subpathway that synthesizes acetoacetate and fumarate from L-phenylalanine.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Phenylalanine-4-hydroxylase (Pah)
  2. Tyrosine aminotransferase (Tat)
  3. 4-hydroxyphenylpyruvate dioxygenase (Hpd)
  4. no protein annotated in this organism
  5. Maleylacetoacetate isomerase (Gstz1)
  6. Fumarylacetoacetase (Fah)
This subpathway is part of the pathway L-phenylalanine degradation, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetoacetate and fumarate from L-phenylalanine, the pathway L-phenylalanine degradation and in Amino-acid degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi126 – 1261CalciumBy similarity
Binding sitei128 – 1281SubstrateBy similarity
Active sitei133 – 1331Proton acceptorCurated
Binding sitei142 – 1421SubstrateBy similarity
Metal bindingi199 – 1991CalciumBy similarity
Metal bindingi201 – 2011CalciumBy similarity
Metal bindingi233 – 2331CalciumBy similarity
Metal bindingi233 – 2331MagnesiumBy similarity
Binding sitei240 – 2401SubstrateBy similarity
Binding sitei244 – 2441SubstrateBy similarity
Metal bindingi253 – 2531MagnesiumBy similarity
Metal bindingi257 – 2571MagnesiumBy similarity
Binding sitei350 – 3501SubstrateBy similarity

GO - Molecular functioni

  • fumarylacetoacetase activity Source: RGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Phenylalanine catabolism, Tyrosine catabolism

Keywords - Ligandi

Calcium, Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00139; UER00341.

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarylacetoacetase (EC:3.7.1.2By similarity)
Short name:
FAA
Alternative name(s):
Beta-diketonase
Fumarylacetoacetate hydrolase
Gene namesi
Name:Fah
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi61932. Fah.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 419418FumarylacetoacetasePRO_0000156827Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei84 – 841PhosphoserineCombined sources
Modified residuei92 – 921PhosphoserineCombined sources
Modified residuei309 – 3091PhosphoserineBy similarity
Modified residuei417 – 4171PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiP25093.

PTM databases

iPTMnetiP25093.
PhosphoSiteiP25093.

Expressioni

Tissue specificityi

Mainly in liver and kidney.1 Publication

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP25093.
SMRiP25093. Positions 1-417.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the FAH family.Curated

Phylogenomic databases

HOGENOMiHOG000256845.
HOVERGENiHBG001919.
InParanoidiP25093.
KOiK01555.
PhylomeDBiP25093.

Family and domain databases

Gene3Di2.30.30.230. 1 hit.
3.90.850.10. 1 hit.
InterProiIPR005959. Fumarylacetoacetase.
IPR011234. Fumarylacetoacetase_C-rel.
IPR015377. Fumarylacetoacetase_N.
[Graphical view]
PANTHERiPTHR11820:SF1. PTHR11820:SF1. 2 hits.
PfamiPF01557. FAA_hydrolase. 1 hit.
PF09298. FAA_hydrolase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56529. SSF56529. 1 hit.
SSF63433. SSF63433. 1 hit.
TIGRFAMsiTIGR01266. fum_ac_acetase. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25093-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFIPVAEDS DFPIQNLPYG VFSTQSNPKP RIGVAIGDQI LDLSVIKHLF
60 70 80 90 100
TGPVLSKHQH VFDETTLNSF MGLGQAAWKE ARASLQNLLS ASQAQLRDDK
110 120 130 140 150
ELRQRAFTSQ ASATMHLPAT IGDYTDFYSS LQHATNVGIM FRGKENALLP
160 170 180 190 200
NWLHLPVGYH GRASSVVVSG TPIRRPMGQM RPDNSKPPVY GASKRLDMEL
210 220 230 240 250
EMAFFVGPGN RFGEPIPISK AQEHIFGMVL MNDWSARDIQ QWEYVPLGPF
260 270 280 290 300
LGKSFGTTIS PWVVPMDALM PFVVPNPKQD PKPLPYLCHS QPYTFDINLS
310 320 330 340 350
VALKGEGMSQ AATICRSNFK HMYWTILQQL THHSVNGCNL RPGDLLASGT
360 370 380 390 400
ISGSDPESFG SMLELSWKGT KAIDVGQGQT RTFLLDGDEV IITGHCQGDG
410
YRVGFGQCAG KVLPALSPA
Length:419
Mass (Da):45,976
Last modified:May 1, 1992 - v1
Checksum:iAC099689591C6176
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77694 mRNA. Translation: AAA41142.1.
BC076381 mRNA. Translation: AAH76381.1.
PIRiJH0467.
RefSeqiNP_058877.1. NM_017181.2.
UniGeneiRn.9195.

Genome annotation databases

GeneIDi29383.
KEGGirno:29383.
UCSCiRGD:61932. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77694 mRNA. Translation: AAA41142.1.
BC076381 mRNA. Translation: AAH76381.1.
PIRiJH0467.
RefSeqiNP_058877.1. NM_017181.2.
UniGeneiRn.9195.

3D structure databases

ProteinModelPortaliP25093.
SMRiP25093. Positions 1-417.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiP25093.
PhosphoSiteiP25093.

Proteomic databases

PRIDEiP25093.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29383.
KEGGirno:29383.
UCSCiRGD:61932. rat.

Organism-specific databases

CTDi2184.
RGDi61932. Fah.

Phylogenomic databases

HOGENOMiHOG000256845.
HOVERGENiHBG001919.
InParanoidiP25093.
KOiK01555.
PhylomeDBiP25093.

Enzyme and pathway databases

UniPathwayiUPA00139; UER00341.

Miscellaneous databases

PROiP25093.

Family and domain databases

Gene3Di2.30.30.230. 1 hit.
3.90.850.10. 1 hit.
InterProiIPR005959. Fumarylacetoacetase.
IPR011234. Fumarylacetoacetase_C-rel.
IPR015377. Fumarylacetoacetase_N.
[Graphical view]
PANTHERiPTHR11820:SF1. PTHR11820:SF1. 2 hits.
PfamiPF01557. FAA_hydrolase. 1 hit.
PF09298. FAA_hydrolase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56529. SSF56529. 1 hit.
SSF63433. SSF63433. 1 hit.
TIGRFAMsiTIGR01266. fum_ac_acetase. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFAAA_RAT
AccessioniPrimary (citable) accession number: P25093
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: June 8, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.