Reviewed, UniProtKB/Swiss-Prot P25093 (FAAA_RAT)

Names and origin

Protein names

Recommended name:
    Fumarylacetoacetase
      Short name=FAA
    EC=3.7.1.2
Alternative name(s):
    Fumarylacetoacetate hydrolase
    Beta-diketonase

Gene names

Name:

Fah

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	4-fumarylacetoacetate + H2O = acetoacetate + fumarate.
Cofactor	Calcium By similarity. Magnesium By similarity.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 6/6.
Subunit structure	Homodimer.
Tissue specificity	Mainly in liver and kidney.
Sequence similarities	Belongs to the FAH family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Ligand	Calcium Magnesium Metal-binding
Molecular function	Hydrolase
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: UniProtKB-KW tyrosine catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW fumarylacetoacetase activity Ref.1 Traceable author statement. Source: RGD magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 419	418	Fumarylacetoacetase		PRO_0000156827
Sites
	Active site	133	1	Proton acceptor Probable
	Metal binding	126	1	Calcium By similarity
	Metal binding	199	1	Calcium By similarity
	Metal binding	201	1	Calcium By similarity
	Metal binding	233	1	Calcium By similarity
	Metal binding	233	1	Magnesium By similarity
	Metal binding	253	1	Magnesium By similarity
	Metal binding	257	1	Magnesium By similarity
	Binding site	128	1	Substrate By similarity
	Binding site	142	1	Substrate By similarity
	Binding site	240	1	Substrate By similarity
	Binding site	244	1	Substrate By similarity
	Binding site	350	1	Substrate By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P25093-1 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: AC099689591C6176	FASTA	419	45,976
	10 20 30 40 50 60 MSFIPVAEDS DFPIQNLPYG VFSTQSNPKP RIGVAIGDQI LDLSVIKHLF TGPVLSKHQH 70 80 90 100 110 120 VFDETTLNSF MGLGQAAWKE ARASLQNLLS ASQAQLRDDK ELRQRAFTSQ ASATMHLPAT 130 140 150 160 170 180 IGDYTDFYSS LQHATNVGIM FRGKENALLP NWLHLPVGYH GRASSVVVSG TPIRRPMGQM 190 200 210 220 230 240 RPDNSKPPVY GASKRLDMEL EMAFFVGPGN RFGEPIPISK AQEHIFGMVL MNDWSARDIQ 250 260 270 280 290 300 QWEYVPLGPF LGKSFGTTIS PWVVPMDALM PFVVPNPKQD PKPLPYLCHS QPYTFDINLS 310 320 330 340 350 360 VALKGEGMSQ AATICRSNFK HMYWTILQQL THHSVNGCNL RPGDLLASGT ISGSDPESFG 370 380 390 400 410 SMLELSWKGT KAIDVGQGQT RTFLLDGDEV IITGHCQGDG YRVGFGQCAG KVLPALSPA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression analysis of a cDNA encoding fumarylacetoacetate hydrolase: post-transcriptional modulation in rat liver and kidney." Labelle Y., Phaneuf D., Tanguay R.M. Gene 104:197-202(1991) [PubMed: 1916290] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[3]	Lubec G., Afjehi-Sadat L. Submitted (NOV-2006) to UniProtKB Cited for: PROTEIN SEQUENCE OF 32-47; 145-162 AND 382-402, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Spinal cord.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M77694 mRNA. Translation: AAA41142.1. BC076381 mRNA. Translation: AAH76381.1.
IPI	IPI00214480.
PIR	JH0467.
RefSeq	NP_058877.1.
UniGene	Rn.9195
3D structure databases
SMR	P25093. Positions 1-417.
ModBase	Search...
Protein-protein interaction databases
STRING	P25093.
Proteomic databases
PRIDE	P25093.
Genome annotation databases
Ensembl	ENSRNOT00000068167; ENSRNOP00000061132; ENSRNOG00000013223; Rattus norvegicus. [Genome view]
GeneID	29383.
KEGG	rno:29383.
Organism-specific databases
CTD	29383.
RGD	61932. Fah.
Phylogenomic databases
eggNOG	roNOG15538.
HOVERGEN	P25093.
InParanoid	P25093.
Enzyme and pathway databases
BRENDA	3.7.1.2. 248.
Gene expression databases
ArrayExpress	P25093.
Genevestigator	P25093.
GermOnline	ENSRNOG00000013223. Rattus norvegicus.
Family and domain databases
InterPro	IPR005959. Fumarylacetoacetase. IPR002529. Fumarylacetoacetase_C-like. IPR011234. Fumarylacetoacetase_C-rel. IPR015377. Fumarylacetoacetase_N. [Graphical view]
Gene3D	G3DSA:3.90.850.10. Fumarylacetoacetase_C-rel. 1 hit. G3DSA:2.30.30.230. Fumarylacetoacetase_N. 1 hit.
PANTHER	PTHR11820:SF1. Fum_ac_acetase. 1 hit.
Pfam	PF09298. DUF1969. 1 hit. PF01557. FAA_hydrolase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01266. fum_ac_acetase. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	608975.

Entry information

Entry name

FAAA_RAT

Accession

Primary (citable) accession number: P25093

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	February 9, 2010
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)