Reviewed,
UniProtKB/Swiss-Prot P25087 (ERG6_YEAST)
Last modified
June 16, 2009.
Version 88.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Sterol 24-C-methyltransferase EC=2.1.1.41 Alternative name(s): Delta(24)-sterol C-methyltransferase | ||||||||
| Gene names |
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| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||||||
| Taxonomic identifier | 4932 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 383 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Catalyzes the methyl transfer from S-adenosyl-methionine to the C-24 of zymosterol to form fecosterol. |
| Catalytic activity | S-adenosyl-L-methionine + 5-alpha-cholesta-8,24-dien-3-beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5-alpha-cholest-8-en-3-beta-ol. |
| Pathway | Steroid metabolism; ergosterol biosynthesis; ergosterol from zymosterol: step 1/5. |
| Subunit structure | |
| Miscellaneous | Present with 53800 molecules/cell in log phase SD medium. Ref.7 |
| Sequence similarities | Belongs to the methyltransferase superfamily. ERG6 family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Lipid synthesis Steroid biosynthesis Sterol biosynthesis |
| Ligand | S-adenosyl-L-methionine |
| Molecular function | Methyltransferase Transferase |
| PTM | Acetylation Phosphoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | lipid particle Inferred from direct assay. Source: SGD mitochondrial outer membraneInferred from direct assay. Source: SGD |
| Molecular function | protein binding Inferred from physical interaction. Source: IntAct sterol 24-C-methyltransferase activityInferred from direct assay. Source: SGD |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| Q12066 | 1 | EBI-6567,EBI-35877 | ||
| Q12274 | 1 | EBI-6567,EBI-33978 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed | ||||||
| Chain | 2 – 383 | 382 | Sterol 24-C-methyltransferase | PRO_0000124799 | |||||
Amino acid modifications | |||||||||
| Modified residue | 2 | 1 | N-acetylserine Ref.5 | ||||||
| Modified residue | 99 | 1 | Phosphoserine Ref.10 | ||||||
| Modified residue | 373 | 1 | Phosphothreonine Ref.10 | ||||||
| Modified residue | 375 | 1 | Phosphoserine Ref.6 | ||||||
| Modified residue | 378 | 1 | Phosphoserine Ref.6 | ||||||
Experimental info | |||||||||
| Sequence conflict | 380 | 1 | E → EE in AAB31378. Ref.2 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "SED6 is identical to ERG6, and encodes a putative methyltransferase required for ergosterol synthesis." Hardwick K.G., Pelham H.R.B. Yeast 10:265-269(1994) [PubMed: 8203167] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "Mutations in LIS1 (ERG6) gene confer increased sodium and lithium uptake in Saccharomyces cerevisiae." Welihinda A.A., Beavis A.D., Trumbly R.J. Biochim. Biophys. Acta 1193:107-117(1994) [PubMed: 8038180] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [3] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G.Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972. |
| [4] | "Characterization of PDR4, a Saccharomyces cerevisiae gene that confers pleiotropic drug resistance in high-copy number: identity with YAP1, encoding a transcriptional activator." Hussain M., Lenard J. Gene 101:149-152(1991) [PubMed: 2060792] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-258. |
| [5] | "Proteome studies of Saccharomyces cerevisiae: identification and characterization of abundant proteins." Garrels J.I., McLaughlin C.S., Warner J.R., Futcher B., Latter G.I., Kobayashi R., Schwender B., Volpe T., Anderson D.S., Mesquita-Fuentes R., Payne W.E. Electrophoresis 18:1347-1360(1997) [PubMed: 9298649] [Abstract] Cited for: ACETYLATION AT SER-2. |
| [6] | "Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M. Nat. Biotechnol. 20:301-305(2002) [PubMed: 11875433] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-375 AND SER-378, MASS SPECTROMETRY. |
| [7] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [8] | "The ERG28-encoded protein, Erg28p, interacts with both the sterol C-4 demethylation enzyme complex as well as the late biosynthetic protein, the C-24 sterol methyltransferase (Erg6p)." Mo C., Valachovic M., Bard M. Biochim. Biophys. Acta 1686:30-36(2004) [PubMed: 15522820] [Abstract] Cited for: INTERACTION WITH ERG28. |
| [9] | "Erg28p is a key protein in the yeast sterol biosynthetic enzyme complex." Mo C., Bard M. J. Lipid Res. 46:1991-1998(2005) [PubMed: 15995173] [Abstract] Cited for: INTERACTION WITH ERG28. |
| [10] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-99 AND THR-373, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| X74249 Genomic DNA. Translation: CAA52308.1. S72460 Genomic DNA. Translation: AAB31378.1. Z49810 Genomic DNA. Translation: CAA89944.1. X53830 Genomic DNA. Translation: CAA37826.1. | |
| PIR | S42003. |
| RefSeq | NP_013706.1. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP:3816N. |
| IntAct | P25087. 20 interactions. |
2-D gel databases | |
| SWISS-2DPAGE | P25087. |
Proteomic databases | |
| PeptideAtlas | P25087. |
| PRIDE | P25087. |
Genome annotation databases | |
| Ensembl | YML008C. Saccharomyces cerevisiae. [Contig view] |
| GeneID | 855003. |
| GenomeReviews | Gene locus YML008C in contig Z71257_GR. |
| KEGG | sce:YML008C. |
| NMPDR | fig|4932.3.peg.4748. |
Organism-specific databases | |
| CYGD | YML008c. |
| SGD | S000004467. ERG6. |
| Yeast-GFP | Search... |
Phylogenomic databases | |
| HOGENOM | P25087. |
| OMA | P25087. FVKGDFM. |
Enzyme and pathway databases | |
| BRENDA | 2.1.1.41. 250. |
Gene expression databases | |
| GermOnline | YML008C. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR013216. Methyltransf_11. IPR013705. Sterol_MeTrfase_C. [Graphical view] |
| Pfam | PF08241. Methyltransf_11. 1 hit. PF08498. Sterol_MT_C. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 978157. |
Entry information
| Entry name | ERG6_YEAST | ||||||||
| Accession | Primary (citable) accession number: P25087 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome XIII Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names |

Clusters with


