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Protein

Sterol 24-C-methyltransferase

Gene

ERG6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the methyl transfer from S-adenosyl-methionine to the C-24 of zymosterol to form fecosterol.

Catalytic activityi

S-adenosyl-L-methionine + 5-alpha-cholesta-8,24-dien-3-beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5-alpha-cholest-8-en-3-beta-ol.

Pathwayi: ergosterol biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes ergosterol from zymosterol.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Sterol 24-C-methyltransferase (ERG6)
  2. C-8 sterol isomerase (ERG2)
  3. Delta(7)-sterol 5(6)-desaturase (ERG3)
  4. Cytochrome P450 61 (ERG5)
  5. Delta(24(24(1)))-sterol reductase (ERG4)
This subpathway is part of the pathway ergosterol biosynthesis, which is itself part of Steroid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ergosterol from zymosterol, the pathway ergosterol biosynthesis and in Steroid metabolism.

GO - Molecular functioni

  • sterol 24-C-methyltransferase activity Source: SGD

GO - Biological processi

  • ergosterol biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER3O-188.
YEAST:MONOMER3O-188.
BRENDAi2.1.1.41. 984.
SABIO-RKP25087.
UniPathwayiUPA00768; UER00760.

Names & Taxonomyi

Protein namesi
Recommended name:
Sterol 24-C-methyltransferase (EC:2.1.1.41)
Alternative name(s):
Delta(24)-sterol C-methyltransferase
Gene namesi
Name:ERG6
Synonyms:ISE1, LIS1, SED6
Ordered Locus Names:YML008C
ORF Names:YM9571.10C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YML008C.
SGDiS000004467. ERG6.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum Source: SGD
  • lipid particle Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001247992 – 383Sterol 24-C-methyltransferaseAdd BLAST382

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserine1 Publication1
Modified residuei99PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP25087.
PRIDEiP25087.

2D gel databases

SWISS-2DPAGEP25087.

PTM databases

iPTMnetiP25087.

Interactioni

Subunit structurei

Interacts with ERG28.2 Publications

Protein-protein interaction databases

BioGridi35161. 357 interactors.
DIPiDIP-3816N.
IntActiP25087. 16 interactors.
MINTiMINT-537879.

Structurei

3D structure databases

ProteinModelPortaliP25087.
SMRiP25087.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Erg6/SMT family.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00530000069303.
HOGENOMiHOG000171097.
InParanoidiP25087.
KOiK00559.
OMAiFHFCRFS.
OrthoDBiEOG092C2LI4.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR013216. Methyltransf_11.
IPR030384. MeTrfase_SMT.
IPR029063. SAM-dependent_MTases.
IPR013705. Sterol_MeTrfase_C.
[Graphical view]
PfamiPF08241. Methyltransf_11. 1 hit.
PF08498. Sterol_MT_C. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS51685. SAM_MT_ERG6_SMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25087-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSETELRKRQ AQFTRELHGD DIGKKTGLSA LMSKNNSAQK EAVQKYLRNW
60 70 80 90 100
DGRTDKDAEE RRLEDYNEAT HSYYNVVTDF YEYGWGSSFH FSRFYKGESF
110 120 130 140 150
AASIARHEHY LAYKAGIQRG DLVLDVGCGV GGPAREIARF TGCNVIGLNN
160 170 180 190 200
NDYQIAKAKY YAKKYNLSDQ MDFVKGDFMK MDFEENTFDK VYAIEATCHA
210 220 230 240 250
PKLEGVYSEI YKVLKPGGTF AVYEWVMTDK YDENNPEHRK IAYEIELGDG
260 270 280 290 300
IPKMFHVDVA RKALKNCGFE VLVSEDLADN DDEIPWYYPL TGEWKYVQNL
310 320 330 340 350
ANLATFFRTS YLGRQFTTAM VTVMEKLGLA PEGSKEVTAA LENAAVGLVA
360 370 380
GGKSKLFTPM MLFVARKPEN AETPSQTSQE ATQ
Length:383
Mass (Da):43,431
Last modified:January 23, 2007 - v4
Checksum:iC3A2B6DF2C14ED63
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti380E → EE in AAB31378 (PubMed:8038180).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74249 Genomic DNA. Translation: CAA52308.1.
S72460 Genomic DNA. Translation: AAB31378.1.
Z49810 Genomic DNA. Translation: CAA89944.1.
X53830 Genomic DNA. Translation: CAA37826.1.
BK006946 Genomic DNA. Translation: DAA09890.1.
PIRiS42003.
RefSeqiNP_013706.1. NM_001182363.1.

Genome annotation databases

EnsemblFungiiYML008C; YML008C; YML008C.
GeneIDi855003.
KEGGisce:YML008C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74249 Genomic DNA. Translation: CAA52308.1.
S72460 Genomic DNA. Translation: AAB31378.1.
Z49810 Genomic DNA. Translation: CAA89944.1.
X53830 Genomic DNA. Translation: CAA37826.1.
BK006946 Genomic DNA. Translation: DAA09890.1.
PIRiS42003.
RefSeqiNP_013706.1. NM_001182363.1.

3D structure databases

ProteinModelPortaliP25087.
SMRiP25087.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35161. 357 interactors.
DIPiDIP-3816N.
IntActiP25087. 16 interactors.
MINTiMINT-537879.

PTM databases

iPTMnetiP25087.

2D gel databases

SWISS-2DPAGEP25087.

Proteomic databases

MaxQBiP25087.
PRIDEiP25087.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYML008C; YML008C; YML008C.
GeneIDi855003.
KEGGisce:YML008C.

Organism-specific databases

EuPathDBiFungiDB:YML008C.
SGDiS000004467. ERG6.

Phylogenomic databases

GeneTreeiENSGT00530000069303.
HOGENOMiHOG000171097.
InParanoidiP25087.
KOiK00559.
OMAiFHFCRFS.
OrthoDBiEOG092C2LI4.

Enzyme and pathway databases

UniPathwayiUPA00768; UER00760.
BioCyciMetaCyc:MONOMER3O-188.
YEAST:MONOMER3O-188.
BRENDAi2.1.1.41. 984.
SABIO-RKP25087.

Miscellaneous databases

PROiP25087.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR013216. Methyltransf_11.
IPR030384. MeTrfase_SMT.
IPR029063. SAM-dependent_MTases.
IPR013705. Sterol_MeTrfase_C.
[Graphical view]
PfamiPF08241. Methyltransf_11. 1 hit.
PF08498. Sterol_MT_C. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS51685. SAM_MT_ERG6_SMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiERG6_YEAST
AccessioniPrimary (citable) accession number: P25087
Secondary accession number(s): D6VZG6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 154 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 53800 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.