P25087 (ERG6_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sterol 24-C-methyltransferase
EC=2.1.1.41

Alternative name(s):
Delta(24)-sterol C-methyltransferase

Gene names

Name:	ERG6
Synonyms:	ISE1, LIS1, SED6
Ordered Locus Names:	YML008C
ORF Names:	YM9571.10C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	383 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the methyl transfer from S-adenosyl-methionine to the C-24 of zymosterol to form fecosterol.
Catalytic activity	S-adenosyl-L-methionine + 5-alpha-cholesta-8,24-dien-3-beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5-alpha-cholest-8-en-3-beta-ol.
Pathway	Steroid metabolism; ergosterol biosynthesis; ergosterol from zymosterol: step 1/5.
Subunit structure	Interacts with ERG28. Ref.9 Ref.10
Miscellaneous	Present with 53800 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the class I-like SAM-binding methyltransferase superfamily. ERG6 family.

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism Steroid biosynthesis Steroid metabolism Sterol biosynthesis Sterol metabolism
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ergosterol biosynthetic process Inferred from mutant phenotype PubMed 2677674PubMed 6363386. Source: SGD
Cellular_component	endoplasmic reticulum Inferred from direct assay PubMed 11298754PubMed 9450962. Source: SGD lipid particle Inferred from direct assay PubMed 10515935. Source: SGD mitochondrial outer membrane Inferred from direct assay PubMed 16407407. Source: SGD
Molecular_function	sterol 24-C-methyltransferase activity Inferred from direct assay PubMed 18563465. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
YDL089W	Q12066	1	EBI-6567,EBI-35877
YOR097C	Q12274	1	EBI-6567,EBI-33978

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 383	382	Sterol 24-C-methyltransferase		PRO_0000124799
Amino acid modifications
	Modified residue	2	1	N-acetylserine Ref.6
	Modified residue	99	1	Phosphoserine Ref.11
	Modified residue	373	1	Phosphothreonine Ref.11
	Modified residue	375	1	Phosphoserine Ref.7
	Modified residue	378	1	Phosphoserine Ref.7
Experimental info
	Sequence conflict	380	1	E → EE in AAB31378. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P25087 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: C3A2B6DF2C14ED63	FASTA	383	43,431
	10 20 30 40 50 60 MSETELRKRQ AQFTRELHGD DIGKKTGLSA LMSKNNSAQK EAVQKYLRNW DGRTDKDAEE 70 80 90 100 110 120 RRLEDYNEAT HSYYNVVTDF YEYGWGSSFH FSRFYKGESF AASIARHEHY LAYKAGIQRG 130 140 150 160 170 180 DLVLDVGCGV GGPAREIARF TGCNVIGLNN NDYQIAKAKY YAKKYNLSDQ MDFVKGDFMK 190 200 210 220 230 240 MDFEENTFDK VYAIEATCHA PKLEGVYSEI YKVLKPGGTF AVYEWVMTDK YDENNPEHRK 250 260 270 280 290 300 IAYEIELGDG IPKMFHVDVA RKALKNCGFE VLVSEDLADN DDEIPWYYPL TGEWKYVQNL 310 320 330 340 350 360 ANLATFFRTS YLGRQFTTAM VTVMEKLGLA PEGSKEVTAA LENAAVGLVA GGKSKLFTPM 370 380 MLFVARKPEN AETPSQTSQE ATQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"SED6 is identical to ERG6, and encodes a putative methyltransferase required for ergosterol synthesis." Hardwick K.G., Pelham H.R.B. Yeast 10:265-269(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Mutations in LIS1 (ERG6) gene confer increased sodium and lithium uptake in Saccharomyces cerevisiae." Welihinda A.A., Beavis A.D., Trumbly R.J. Biochim. Biophys. Acta 1193:107-117(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Characterization of PDR4, a Saccharomyces cerevisiae gene that confers pleiotropic drug resistance in high-copy number: identity with YAP1, encoding a transcriptional activator." Hussain M., Lenard J. Gene 101:149-152(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-258.
[6]	"Proteome studies of Saccharomyces cerevisiae: identification and characterization of abundant proteins." Garrels J.I., McLaughlin C.S., Warner J.R., Futcher B., Latter G.I., Kobayashi R., Schwender B., Volpe T., Anderson D.S., Mesquita-Fuentes R., Payne W.E. Electrophoresis 18:1347-1360(1997) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION AT SER-2.
[7]	"Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M. Nat. Biotechnol. 20:301-305(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-375 AND SER-378, MASS SPECTROMETRY. Strain: 2124.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"The ERG28-encoded protein, Erg28p, interacts with both the sterol C-4 demethylation enzyme complex as well as the late biosynthetic protein, the C-24 sterol methyltransferase (Erg6p)." Mo C., Valachovic M., Bard M. Biochim. Biophys. Acta 1686:30-36(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ERG28.
[10]	"Erg28p is a key protein in the yeast sterol biosynthetic enzyme complex." Mo C., Bard M. J. Lipid Res. 46:1991-1998(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ERG28.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-99 AND THR-373, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X74249 Genomic DNA. Translation: CAA52308.1. S72460 Genomic DNA. Translation: AAB31378.1. Z49810 Genomic DNA. Translation: CAA89944.1. X53830 Genomic DNA. Translation: CAA37826.1. BK006946 Genomic DNA. Translation: DAA09890.1.
PIR	S42003.
RefSeq	NP_013706.1. NM_001182363.1.
3D structure databases
ProteinModelPortal	P25087.
SMR	P25087. Positions 80-272.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3816N.
IntAct	P25087. 15 interactions.
MINT	MINT-537879.
STRING	4932.YML008C.
2D gel databases
SWISS-2DPAGE	P25087.
Proteomic databases
PaxDb	P25087.
PeptideAtlas	P25087.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YML008C; YML008C; YML008C.
GeneID	855003.
KEGG	sce:YML008C.
Organism-specific databases
SGD	S000004467. ERG6.
Phylogenomic databases
eggNOG	COG0500.
GeneTree	ENSGT00530000069303.
HOGENOM	HOG000171097.
KO	K00559.
OMA	KLFTPMM.
OrthoDB	EOG4HHSB5.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER3O-188.
SABIO-RK	P25087.
UniPathway	UPA00768; UER00760.
Gene expression databases
Genevestigator	P25087.
GermOnline	YML008C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR025810. ERG6. IPR013216. Methyltransf_11. IPR013705. Sterol_MeTrfase_C. [Graphical view]
Pfam	PF08241. Methyltransf_11. 1 hit. PF08498. Sterol_MT_C. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	978157.

Entry information

Entry name

ERG6_YEAST

Accession

Primary (citable) accession number: P25087
Secondary accession number(s): D6VZG6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 124 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program