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P25087

- ERG6_YEAST

UniProt

P25087 - ERG6_YEAST

Protein

Sterol 24-C-methyltransferase

Gene

ERG6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 4 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the methyl transfer from S-adenosyl-methionine to the C-24 of zymosterol to form fecosterol.

    Catalytic activityi

    S-adenosyl-L-methionine + 5-alpha-cholesta-8,24-dien-3-beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5-alpha-cholest-8-en-3-beta-ol.

    Pathwayi

    GO - Molecular functioni

    1. sterol 24-C-methyltransferase activity Source: SGD

    GO - Biological processi

    1. ergosterol biosynthetic process Source: SGD

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER3O-188.
    YEAST:MONOMER3O-188.
    SABIO-RKP25087.
    UniPathwayiUPA00768; UER00760.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sterol 24-C-methyltransferase (EC:2.1.1.41)
    Alternative name(s):
    Delta(24)-sterol C-methyltransferase
    Gene namesi
    Name:ERG6
    Synonyms:ISE1, LIS1, SED6
    Ordered Locus Names:YML008C
    ORF Names:YM9571.10C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    SGDiS000004467. ERG6.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: SGD
    2. lipid particle Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 383382Sterol 24-C-methyltransferasePRO_0000124799Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication
    Modified residuei99 – 991Phosphoserine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP25087.
    PaxDbiP25087.
    PeptideAtlasiP25087.

    2D gel databases

    SWISS-2DPAGEP25087.

    Expressioni

    Gene expression databases

    GenevestigatoriP25087.

    Interactioni

    Subunit structurei

    Interacts with ERG28.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    YDL089WQ120661EBI-6567,EBI-35877
    YOR097CQ122741EBI-6567,EBI-33978

    Protein-protein interaction databases

    BioGridi35161. 355 interactions.
    DIPiDIP-3816N.
    IntActiP25087. 16 interactions.
    MINTiMINT-537879.
    STRINGi4932.YML008C.

    Structurei

    3D structure databases

    ProteinModelPortaliP25087.
    SMRiP25087. Positions 80-279.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. Erg6/SMT family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0500.
    GeneTreeiENSGT00530000069303.
    HOGENOMiHOG000171097.
    KOiK00559.
    OMAiFHFCRFS.
    OrthoDBiEOG7DVDMW.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR025810. ERG6.
    IPR013216. Methyltransf_11.
    IPR029063. SAM-dependent_MTases-like.
    IPR013705. Sterol_MeTrfase_C.
    [Graphical view]
    PfamiPF08241. Methyltransf_11. 1 hit.
    PF08498. Sterol_MT_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 2 hits.
    PROSITEiPS51685. SAM_MT_ERG6_SMT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P25087-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSETELRKRQ AQFTRELHGD DIGKKTGLSA LMSKNNSAQK EAVQKYLRNW    50
    DGRTDKDAEE RRLEDYNEAT HSYYNVVTDF YEYGWGSSFH FSRFYKGESF 100
    AASIARHEHY LAYKAGIQRG DLVLDVGCGV GGPAREIARF TGCNVIGLNN 150
    NDYQIAKAKY YAKKYNLSDQ MDFVKGDFMK MDFEENTFDK VYAIEATCHA 200
    PKLEGVYSEI YKVLKPGGTF AVYEWVMTDK YDENNPEHRK IAYEIELGDG 250
    IPKMFHVDVA RKALKNCGFE VLVSEDLADN DDEIPWYYPL TGEWKYVQNL 300
    ANLATFFRTS YLGRQFTTAM VTVMEKLGLA PEGSKEVTAA LENAAVGLVA 350
    GGKSKLFTPM MLFVARKPEN AETPSQTSQE ATQ 383
    Length:383
    Mass (Da):43,431
    Last modified:January 23, 2007 - v4
    Checksum:iC3A2B6DF2C14ED63
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti380 – 3801E → EE in AAB31378. (PubMed:8038180)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X74249 Genomic DNA. Translation: CAA52308.1.
    S72460 Genomic DNA. Translation: AAB31378.1.
    Z49810 Genomic DNA. Translation: CAA89944.1.
    X53830 Genomic DNA. Translation: CAA37826.1.
    BK006946 Genomic DNA. Translation: DAA09890.1.
    PIRiS42003.
    RefSeqiNP_013706.1. NM_001182363.1.

    Genome annotation databases

    EnsemblFungiiYML008C; YML008C; YML008C.
    GeneIDi855003.
    KEGGisce:YML008C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X74249 Genomic DNA. Translation: CAA52308.1 .
    S72460 Genomic DNA. Translation: AAB31378.1 .
    Z49810 Genomic DNA. Translation: CAA89944.1 .
    X53830 Genomic DNA. Translation: CAA37826.1 .
    BK006946 Genomic DNA. Translation: DAA09890.1 .
    PIRi S42003.
    RefSeqi NP_013706.1. NM_001182363.1.

    3D structure databases

    ProteinModelPortali P25087.
    SMRi P25087. Positions 80-279.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35161. 355 interactions.
    DIPi DIP-3816N.
    IntActi P25087. 16 interactions.
    MINTi MINT-537879.
    STRINGi 4932.YML008C.

    2D gel databases

    SWISS-2DPAGE P25087.

    Proteomic databases

    MaxQBi P25087.
    PaxDbi P25087.
    PeptideAtlasi P25087.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YML008C ; YML008C ; YML008C .
    GeneIDi 855003.
    KEGGi sce:YML008C.

    Organism-specific databases

    SGDi S000004467. ERG6.

    Phylogenomic databases

    eggNOGi COG0500.
    GeneTreei ENSGT00530000069303.
    HOGENOMi HOG000171097.
    KOi K00559.
    OMAi FHFCRFS.
    OrthoDBi EOG7DVDMW.

    Enzyme and pathway databases

    UniPathwayi UPA00768 ; UER00760 .
    BioCyci MetaCyc:MONOMER3O-188.
    YEAST:MONOMER3O-188.
    SABIO-RK P25087.

    Miscellaneous databases

    NextBioi 978157.

    Gene expression databases

    Genevestigatori P25087.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR025810. ERG6.
    IPR013216. Methyltransf_11.
    IPR029063. SAM-dependent_MTases-like.
    IPR013705. Sterol_MeTrfase_C.
    [Graphical view ]
    Pfami PF08241. Methyltransf_11. 1 hit.
    PF08498. Sterol_MT_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 2 hits.
    PROSITEi PS51685. SAM_MT_ERG6_SMT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "SED6 is identical to ERG6, and encodes a putative methyltransferase required for ergosterol synthesis."
      Hardwick K.G., Pelham H.R.B.
      Yeast 10:265-269(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Mutations in LIS1 (ERG6) gene confer increased sodium and lithium uptake in Saccharomyces cerevisiae."
      Welihinda A.A., Beavis A.D., Trumbly R.J.
      Biochim. Biophys. Acta 1193:107-117(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Characterization of PDR4, a Saccharomyces cerevisiae gene that confers pleiotropic drug resistance in high-copy number: identity with YAP1, encoding a transcriptional activator."
      Hussain M., Lenard J.
      Gene 101:149-152(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-258.
    6. "Proteome studies of Saccharomyces cerevisiae: identification and characterization of abundant proteins."
      Garrels J.I., McLaughlin C.S., Warner J.R., Futcher B., Latter G.I., Kobayashi R., Schwender B., Volpe T., Anderson D.S., Mesquita-Fuentes R., Payne W.E.
      Electrophoresis 18:1347-1360(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION AT SER-2.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "The ERG28-encoded protein, Erg28p, interacts with both the sterol C-4 demethylation enzyme complex as well as the late biosynthetic protein, the C-24 sterol methyltransferase (Erg6p)."
      Mo C., Valachovic M., Bard M.
      Biochim. Biophys. Acta 1686:30-36(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ERG28.
    9. "Erg28p is a key protein in the yeast sterol biosynthetic enzyme complex."
      Mo C., Bard M.
      J. Lipid Res. 46:1991-1998(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ERG28.
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-99, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiERG6_YEAST
    AccessioniPrimary (citable) accession number: P25087
    Secondary accession number(s): D6VZG6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 136 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 53800 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3