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Protein

Sterol 24-C-methyltransferase

Gene

ERG6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the methyl transfer from S-adenosyl-methionine to the C-24 of zymosterol to form fecosterol.

Catalytic activityi

S-adenosyl-L-methionine + 5-alpha-cholesta-8,24-dien-3-beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5-alpha-cholest-8-en-3-beta-ol.

Pathwayi

GO - Molecular functioni

  1. sterol 24-C-methyltransferase activity Source: SGD

GO - Biological processi

  1. ergosterol biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER3O-188.
YEAST:MONOMER3O-188.
BRENDAi2.1.1.41. 984.
SABIO-RKP25087.
UniPathwayiUPA00768; UER00760.

Names & Taxonomyi

Protein namesi
Recommended name:
Sterol 24-C-methyltransferase (EC:2.1.1.41)
Alternative name(s):
Delta(24)-sterol C-methyltransferase
Gene namesi
Name:ERG6
Synonyms:ISE1, LIS1, SED6
Ordered Locus Names:YML008C
ORF Names:YM9571.10C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XIII

Organism-specific databases

SGDiS000004467. ERG6.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: SGD
  2. lipid particle Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 383382Sterol 24-C-methyltransferasePRO_0000124799Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei99 – 991Phosphoserine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP25087.
PaxDbiP25087.
PeptideAtlasiP25087.

2D gel databases

SWISS-2DPAGEP25087.

Expressioni

Gene expression databases

GenevestigatoriP25087.

Interactioni

Subunit structurei

Interacts with ERG28.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
YDL089WQ120661EBI-6567,EBI-35877
YOR097CQ122741EBI-6567,EBI-33978

Protein-protein interaction databases

BioGridi35161. 356 interactions.
DIPiDIP-3816N.
IntActiP25087. 16 interactions.
MINTiMINT-537879.
STRINGi4932.YML008C.

Structurei

3D structure databases

SMRiP25087. Positions 80-279.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Erg6/SMT family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0500.
GeneTreeiENSGT00530000069303.
HOGENOMiHOG000171097.
InParanoidiP25087.
KOiK00559.
OMAiFHFCRFS.
OrthoDBiEOG7DVDMW.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR013216. Methyltransf_11.
IPR030384. MeTrfase_SMT.
IPR029063. SAM-dependent_MTases.
IPR013705. Sterol_MeTrfase_C.
[Graphical view]
PfamiPF08241. Methyltransf_11. 1 hit.
PF08498. Sterol_MT_C. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS51685. SAM_MT_ERG6_SMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25087-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSETELRKRQ AQFTRELHGD DIGKKTGLSA LMSKNNSAQK EAVQKYLRNW
60 70 80 90 100
DGRTDKDAEE RRLEDYNEAT HSYYNVVTDF YEYGWGSSFH FSRFYKGESF
110 120 130 140 150
AASIARHEHY LAYKAGIQRG DLVLDVGCGV GGPAREIARF TGCNVIGLNN
160 170 180 190 200
NDYQIAKAKY YAKKYNLSDQ MDFVKGDFMK MDFEENTFDK VYAIEATCHA
210 220 230 240 250
PKLEGVYSEI YKVLKPGGTF AVYEWVMTDK YDENNPEHRK IAYEIELGDG
260 270 280 290 300
IPKMFHVDVA RKALKNCGFE VLVSEDLADN DDEIPWYYPL TGEWKYVQNL
310 320 330 340 350
ANLATFFRTS YLGRQFTTAM VTVMEKLGLA PEGSKEVTAA LENAAVGLVA
360 370 380
GGKSKLFTPM MLFVARKPEN AETPSQTSQE ATQ
Length:383
Mass (Da):43,431
Last modified:January 23, 2007 - v4
Checksum:iC3A2B6DF2C14ED63
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti380 – 3801E → EE in AAB31378 (PubMed:8038180).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74249 Genomic DNA. Translation: CAA52308.1.
S72460 Genomic DNA. Translation: AAB31378.1.
Z49810 Genomic DNA. Translation: CAA89944.1.
X53830 Genomic DNA. Translation: CAA37826.1.
BK006946 Genomic DNA. Translation: DAA09890.1.
PIRiS42003.
RefSeqiNP_013706.1. NM_001182363.1.

Genome annotation databases

EnsemblFungiiYML008C; YML008C; YML008C.
GeneIDi855003.
KEGGisce:YML008C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74249 Genomic DNA. Translation: CAA52308.1.
S72460 Genomic DNA. Translation: AAB31378.1.
Z49810 Genomic DNA. Translation: CAA89944.1.
X53830 Genomic DNA. Translation: CAA37826.1.
BK006946 Genomic DNA. Translation: DAA09890.1.
PIRiS42003.
RefSeqiNP_013706.1. NM_001182363.1.

3D structure databases

SMRiP25087. Positions 80-279.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35161. 356 interactions.
DIPiDIP-3816N.
IntActiP25087. 16 interactions.
MINTiMINT-537879.
STRINGi4932.YML008C.

2D gel databases

SWISS-2DPAGEP25087.

Proteomic databases

MaxQBiP25087.
PaxDbiP25087.
PeptideAtlasiP25087.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYML008C; YML008C; YML008C.
GeneIDi855003.
KEGGisce:YML008C.

Organism-specific databases

SGDiS000004467. ERG6.

Phylogenomic databases

eggNOGiCOG0500.
GeneTreeiENSGT00530000069303.
HOGENOMiHOG000171097.
InParanoidiP25087.
KOiK00559.
OMAiFHFCRFS.
OrthoDBiEOG7DVDMW.

Enzyme and pathway databases

UniPathwayiUPA00768; UER00760.
BioCyciMetaCyc:MONOMER3O-188.
YEAST:MONOMER3O-188.
BRENDAi2.1.1.41. 984.
SABIO-RKP25087.

Miscellaneous databases

NextBioi978157.

Gene expression databases

GenevestigatoriP25087.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR013216. Methyltransf_11.
IPR030384. MeTrfase_SMT.
IPR029063. SAM-dependent_MTases.
IPR013705. Sterol_MeTrfase_C.
[Graphical view]
PfamiPF08241. Methyltransf_11. 1 hit.
PF08498. Sterol_MT_C. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS51685. SAM_MT_ERG6_SMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "SED6 is identical to ERG6, and encodes a putative methyltransferase required for ergosterol synthesis."
    Hardwick K.G., Pelham H.R.B.
    Yeast 10:265-269(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Mutations in LIS1 (ERG6) gene confer increased sodium and lithium uptake in Saccharomyces cerevisiae."
    Welihinda A.A., Beavis A.D., Trumbly R.J.
    Biochim. Biophys. Acta 1193:107-117(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Characterization of PDR4, a Saccharomyces cerevisiae gene that confers pleiotropic drug resistance in high-copy number: identity with YAP1, encoding a transcriptional activator."
    Hussain M., Lenard J.
    Gene 101:149-152(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-258.
  6. "Proteome studies of Saccharomyces cerevisiae: identification and characterization of abundant proteins."
    Garrels J.I., McLaughlin C.S., Warner J.R., Futcher B., Latter G.I., Kobayashi R., Schwender B., Volpe T., Anderson D.S., Mesquita-Fuentes R., Payne W.E.
    Electrophoresis 18:1347-1360(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION AT SER-2.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "The ERG28-encoded protein, Erg28p, interacts with both the sterol C-4 demethylation enzyme complex as well as the late biosynthetic protein, the C-24 sterol methyltransferase (Erg6p)."
    Mo C., Valachovic M., Bard M.
    Biochim. Biophys. Acta 1686:30-36(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ERG28.
  9. "Erg28p is a key protein in the yeast sterol biosynthetic enzyme complex."
    Mo C., Bard M.
    J. Lipid Res. 46:1991-1998(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ERG28.
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-99, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiERG6_YEAST
AccessioniPrimary (citable) accession number: P25087
Secondary accession number(s): D6VZG6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2007
Last modified: April 1, 2015
This is version 139 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 53800 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.