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P25087 (ERG6_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sterol 24-C-methyltransferase

EC=2.1.1.41
Alternative name(s):
Delta(24)-sterol C-methyltransferase
Gene names
Name:ERG6
Synonyms:ISE1, LIS1, SED6
Ordered Locus Names:YML008C
ORF Names:YM9571.10C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length383 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the methyl transfer from S-adenosyl-methionine to the C-24 of zymosterol to form fecosterol.

Catalytic activity

S-adenosyl-L-methionine + 5-alpha-cholesta-8,24-dien-3-beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5-alpha-cholest-8-en-3-beta-ol.

Pathway

Steroid metabolism; ergosterol biosynthesis; ergosterol from zymosterol: step 1/5.

Subunit structure

Interacts with ERG28. Ref.8 Ref.9

Miscellaneous

Present with 53800 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. Erg6/SMT family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

YDL089WQ120661EBI-6567,EBI-35877
YOR097CQ122741EBI-6567,EBI-33978

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 383382Sterol 24-C-methyltransferase
PRO_0000124799

Amino acid modifications

Modified residue21N-acetylserine Ref.6
Modified residue991Phosphoserine Ref.10

Experimental info

Sequence conflict3801E → EE in AAB31378. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P25087 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: C3A2B6DF2C14ED63

FASTA38343,431
        10         20         30         40         50         60 
MSETELRKRQ AQFTRELHGD DIGKKTGLSA LMSKNNSAQK EAVQKYLRNW DGRTDKDAEE 

        70         80         90        100        110        120 
RRLEDYNEAT HSYYNVVTDF YEYGWGSSFH FSRFYKGESF AASIARHEHY LAYKAGIQRG 

       130        140        150        160        170        180 
DLVLDVGCGV GGPAREIARF TGCNVIGLNN NDYQIAKAKY YAKKYNLSDQ MDFVKGDFMK 

       190        200        210        220        230        240 
MDFEENTFDK VYAIEATCHA PKLEGVYSEI YKVLKPGGTF AVYEWVMTDK YDENNPEHRK 

       250        260        270        280        290        300 
IAYEIELGDG IPKMFHVDVA RKALKNCGFE VLVSEDLADN DDEIPWYYPL TGEWKYVQNL 

       310        320        330        340        350        360 
ANLATFFRTS YLGRQFTTAM VTVMEKLGLA PEGSKEVTAA LENAAVGLVA GGKSKLFTPM 

       370        380 
MLFVARKPEN AETPSQTSQE ATQ 

« Hide

References

« Hide 'large scale' references
[1]"SED6 is identical to ERG6, and encodes a putative methyltransferase required for ergosterol synthesis."
Hardwick K.G., Pelham H.R.B.
Yeast 10:265-269(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Mutations in LIS1 (ERG6) gene confer increased sodium and lithium uptake in Saccharomyces cerevisiae."
Welihinda A.A., Beavis A.D., Trumbly R.J.
Biochim. Biophys. Acta 1193:107-117(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Characterization of PDR4, a Saccharomyces cerevisiae gene that confers pleiotropic drug resistance in high-copy number: identity with YAP1, encoding a transcriptional activator."
Hussain M., Lenard J.
Gene 101:149-152(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-258.
[6]"Proteome studies of Saccharomyces cerevisiae: identification and characterization of abundant proteins."
Garrels J.I., McLaughlin C.S., Warner J.R., Futcher B., Latter G.I., Kobayashi R., Schwender B., Volpe T., Anderson D.S., Mesquita-Fuentes R., Payne W.E.
Electrophoresis 18:1347-1360(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION AT SER-2.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"The ERG28-encoded protein, Erg28p, interacts with both the sterol C-4 demethylation enzyme complex as well as the late biosynthetic protein, the C-24 sterol methyltransferase (Erg6p)."
Mo C., Valachovic M., Bard M.
Biochim. Biophys. Acta 1686:30-36(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ERG28.
[9]"Erg28p is a key protein in the yeast sterol biosynthetic enzyme complex."
Mo C., Bard M.
J. Lipid Res. 46:1991-1998(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ERG28.
[10]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-99, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X74249 Genomic DNA. Translation: CAA52308.1.
S72460 Genomic DNA. Translation: AAB31378.1.
Z49810 Genomic DNA. Translation: CAA89944.1.
X53830 Genomic DNA. Translation: CAA37826.1.
BK006946 Genomic DNA. Translation: DAA09890.1.
PIRS42003.
RefSeqNP_013706.1. NM_001182363.1.

3D structure databases

ProteinModelPortalP25087.
SMRP25087. Positions 67-370.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35161. 353 interactions.
DIPDIP-3816N.
IntActP25087. 16 interactions.
MINTMINT-537879.
STRING4932.YML008C.

2D gel databases

SWISS-2DPAGEP25087.

Proteomic databases

PaxDbP25087.
PeptideAtlasP25087.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYML008C; YML008C; YML008C.
GeneID855003.
KEGGsce:YML008C.

Organism-specific databases

SGDS000004467. ERG6.

Phylogenomic databases

eggNOGCOG0500.
GeneTreeENSGT00530000069303.
HOGENOMHOG000171097.
KOK00559.
OMAFHFCRFS.
OrthoDBEOG7DVDMW.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER3O-188.
YEAST:MONOMER3O-188.
SABIO-RKP25087.
UniPathwayUPA00768; UER00760.

Gene expression databases

GenevestigatorP25087.

Family and domain databases

InterProIPR025810. ERG6.
IPR013216. Methyltransf_11.
IPR013705. Sterol_MeTrfase_C.
[Graphical view]
PfamPF08241. Methyltransf_11. 1 hit.
PF08498. Sterol_MT_C. 1 hit.
[Graphical view]
PROSITEPS51685. SAM_MT_ERG6_SMT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio978157.

Entry information

Entry nameERG6_YEAST
AccessionPrimary (citable) accession number: P25087
Secondary accession number(s): D6VZG6
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2007
Last modified: March 19, 2014
This is version 132 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways