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Protein

Interleukin-1 receptor antagonist protein

Gene

Il1rn

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity.

GO - Molecular functioni

  1. cytokine activity Source: GO_Central
  2. interleukin-1, Type II receptor binding Source: MGI
  3. interleukin-1, Type I receptor binding Source: MGI
  4. interleukin-1 receptor antagonist activity Source: MGI
  5. interleukin-1 receptor binding Source: MGI
  6. interleukin-1 Type II receptor antagonist activity Source: MGI
  7. interleukin-1 Type I receptor antagonist activity Source: MGI

GO - Biological processi

  1. acute-phase response Source: MGI
  2. carboxylic acid metabolic process Source: Ensembl
  3. cellular response to norepinephrine stimulus Source: Ensembl
  4. chronic inflammatory response to antigenic stimulus Source: Ensembl
  5. cytokine-mediated signaling pathway Source: GO_Central
  6. female pregnancy Source: Ensembl
  7. fever generation Source: Ensembl
  8. insulin secretion Source: MGI
  9. lipid metabolic process Source: MGI
  10. memory Source: Ensembl
  11. negative regulation of apoptotic process Source: Ensembl
  12. negative regulation of cell migration Source: Ensembl
  13. negative regulation of cytokine-mediated signaling pathway Source: MGI
  14. negative regulation of glutamate secretion Source: Ensembl
  15. negative regulation of heterotypic cell-cell adhesion Source: MGI
  16. negative regulation of interleukin-1-mediated signaling pathway Source: MGI
  17. negative regulation of membrane potential Source: Ensembl
  18. positive regulation of JUN kinase activity Source: Ensembl
  19. response to activity Source: Ensembl
  20. response to drug Source: Ensembl
  21. response to glucocorticoid Source: MGI
  22. response to interleukin-1 Source: Ensembl
  23. response to interleukin-4 Source: Ensembl
  24. response to lipopolysaccharide Source: Ensembl
  25. response to starvation Source: Ensembl
  26. sensory perception of pain Source: Ensembl
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_337033. Interleukin-1 signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Interleukin-1 receptor antagonist protein
Short name:
IL-1RN
Short name:
IL-1ra
Short name:
IRAP
Alternative name(s):
IL1 inhibitor
Gene namesi
Name:Il1rn
Synonyms:Il-1ra
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:96547. Il1rn.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. extracellular region Source: MGI
  3. extracellular space Source: MGI
  4. extracellular vesicular exosome Source: MGI
  5. vesicle Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626By similarityAdd
BLAST
Chaini27 – 178152Interleukin-1 receptor antagonist proteinPRO_0000015330Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi92 ↔ 142By similarity
Glycosylationi110 – 1101N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP25085.
PaxDbiP25085.
PRIDEiP25085.

Expressioni

Gene expression databases

BgeeiP25085.
CleanExiMM_IL1RN.
ExpressionAtlasiP25085. baseline and differential.
GenevestigatoriP25085.

Structurei

3D structure databases

ProteinModelPortaliP25085.
SMRiP25085. Positions 35-177.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the IL-1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG46710.
HOGENOMiHOG000231506.
HOVERGENiHBG052099.
InParanoidiP25085.
KOiK05481.
OMAiIHGGKLC.
OrthoDBiEOG7X6M1M.
PhylomeDBiP25085.
TreeFamiTF300203.

Family and domain databases

InterProiIPR008996. Cytokine_IL1-like.
IPR020877. IL-1_CS.
IPR000975. IL-1_fam.
IPR027166. IL-1RA.
IPR003297. IL-1RA/IL-36.
[Graphical view]
PANTHERiPTHR10078:SF6. PTHR10078:SF6. 1 hit.
PfamiPF00340. IL1. 1 hit.
[Graphical view]
PRINTSiPR00264. INTERLEUKIN1.
PR01360. INTRLEUKIN1X.
SUPFAMiSSF50353. SSF50353. 1 hit.
PROSITEiPS00253. INTERLEUKIN_1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P25085-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEICWGPYSH LISLLLILLF HSEAACRPSG KRPCKMQAFR IWDTNQKTFY
60 70 80 90 100
LRNNQLIAGY LQGPNIKLEE KIDMVPIDLH SVFLGIHGGK LCLSCAKSGD
110 120 130 140 150
DIKLQLEEVN ITDLSKNKEE DKRFTFIRSE KGPTTSFESA ACPGWFLCTT
160 170
LEADRPVSLT NTPEEPLIVT KFYFQEDQ
Length:178
Mass (Da):20,274
Last modified:May 1, 1992 - v1
Checksum:i84AA002A3119C024
GO
Isoform 2 (identifier: P25085-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-21: MEICWGPYSHLISLLLILLFH → MA

Show »
Length:159
Mass (Da):17,996
Checksum:iA7CD35D137846222
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2121MEICW…ILLFH → MA in isoform 2. 1 PublicationVSP_002652Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74294 mRNA. Translation: AAA39309.1.
M64404 mRNA. Translation: AAA39277.1.
L32838 Genomic DNA. Translation: AAA20576.1.
AF001795 mRNA. Translation: AAC15251.1.
M57525 mRNA. Translation: AAA39278.1.
M63100 Genomic DNA. Translation: AAA39310.1.
S64082 mRNA. Translation: AAB20265.1.
CCDSiCCDS15736.1. [P25085-2]
CCDS38064.1. [P25085-1]
PIRiA44610.
RefSeqiNP_001034790.1. NM_001039701.3. [P25085-1]
NP_112444.1. NM_031167.5. [P25085-2]
UniGeneiMm.882.

Genome annotation databases

EnsembliENSMUST00000114482; ENSMUSP00000110126; ENSMUSG00000026981. [P25085-1]
ENSMUST00000114487; ENSMUSP00000110131; ENSMUSG00000026981. [P25085-2]
GeneIDi16181.
KEGGimmu:16181.
UCSCiuc008iox.2. mouse. [P25085-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74294 mRNA. Translation: AAA39309.1.
M64404 mRNA. Translation: AAA39277.1.
L32838 Genomic DNA. Translation: AAA20576.1.
AF001795 mRNA. Translation: AAC15251.1.
M57525 mRNA. Translation: AAA39278.1.
M63100 Genomic DNA. Translation: AAA39310.1.
S64082 mRNA. Translation: AAB20265.1.
CCDSiCCDS15736.1. [P25085-2]
CCDS38064.1. [P25085-1]
PIRiA44610.
RefSeqiNP_001034790.1. NM_001039701.3. [P25085-1]
NP_112444.1. NM_031167.5. [P25085-2]
UniGeneiMm.882.

3D structure databases

ProteinModelPortaliP25085.
SMRiP25085. Positions 35-177.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

MaxQBiP25085.
PaxDbiP25085.
PRIDEiP25085.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000114482; ENSMUSP00000110126; ENSMUSG00000026981. [P25085-1]
ENSMUST00000114487; ENSMUSP00000110131; ENSMUSG00000026981. [P25085-2]
GeneIDi16181.
KEGGimmu:16181.
UCSCiuc008iox.2. mouse. [P25085-1]

Organism-specific databases

CTDi3557.
MGIiMGI:96547. Il1rn.

Phylogenomic databases

eggNOGiNOG46710.
HOGENOMiHOG000231506.
HOVERGENiHBG052099.
InParanoidiP25085.
KOiK05481.
OMAiIHGGKLC.
OrthoDBiEOG7X6M1M.
PhylomeDBiP25085.
TreeFamiTF300203.

Enzyme and pathway databases

ReactomeiREACT_337033. Interleukin-1 signaling.

Miscellaneous databases

NextBioi289059.
PROiP25085.
SOURCEiSearch...

Gene expression databases

BgeeiP25085.
CleanExiMM_IL1RN.
ExpressionAtlasiP25085. baseline and differential.
GenevestigatoriP25085.

Family and domain databases

InterProiIPR008996. Cytokine_IL1-like.
IPR020877. IL-1_CS.
IPR000975. IL-1_fam.
IPR027166. IL-1RA.
IPR003297. IL-1RA/IL-36.
[Graphical view]
PANTHERiPTHR10078:SF6. PTHR10078:SF6. 1 hit.
PfamiPF00340. IL1. 1 hit.
[Graphical view]
PRINTSiPR00264. INTERLEUKIN1.
PR01360. INTRLEUKIN1X.
SUPFAMiSSF50353. SSF50353. 1 hit.
PROSITEiPS00253. INTERLEUKIN_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Mouse IL-1 receptor antagonist protein. Molecular characterization, gene mapping, and expression of mRNA in vitro and in vivo."
    Zahedi K., Seldin M.F., Rits M., Ezekowitz R.A., Whitehead A.S.
    J. Immunol. 146:4228-4233(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1).
  2. "Cloning and expression of murine interleukin-1 receptor antagonist in macrophages stimulated by colony-stimulating factor 1."
    Matsushime H., Roussel M.F., Matsushima K., Hishinuma A., Sherr C.J.
    Blood 78:616-623(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. "The mouse interleukin 1 receptor antagonist protein: gene structure and regulation in vitro."
    Zahedi K.A., Uhlar C.M., Rits M., Prada A.E., Whitehead A.S.
    Cytokine 6:1-9(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
    Strain: Swiss.
  4. "Mouse IL-1 receptor antagonist isoforms: complementary DNA cloning and protein expression of intracellular isoform and tissue distribution of secreted and intracellular IL-1 receptor antagonist in vivo."
    Gabay C., Porter B., Fantuzzi G., Arend W.P.
    J. Immunol. 159:5905-5913(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Strain: FVB X DBA/1 LACJ.
  5. "Interleukin 1 receptor antagonist is a member of the interleukin 1 gene family: evolution of a cytokine control mechanism."
    Eisenberg S.P., Brewer M.T., Verderber E., Heimdal P., Brandhuber B.J., Thompson R.C.
    Proc. Natl. Acad. Sci. U.S.A. 88:5232-5236(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 7-178.
  6. "Cloning, heterologous expression and characterization of murine interleukin 1 receptor antagonist protein."
    Shuck M.E., Eessalu T.E., Tracey D.E., Bienkowski M.J.
    Eur. J. Immunol. 21:2775-2780(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 23-178.

Entry informationi

Entry nameiIL1RA_MOUSE
AccessioniPrimary (citable) accession number: P25085
Secondary accession number(s): O70207
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: April 1, 2015
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.