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Protein

Transcriptional activator protein LasR

Gene

lasR

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator of elastase structural gene (LasB). Binds to the PAI autoinducer.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi194 – 213H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • transcription factor activity, sequence-specific DNA binding Source: PseudoCAP

GO - Biological processi

  • pathogenesis Source: PseudoCAP
  • positive regulation of elastin biosynthetic process Source: PseudoCAP
  • positive regulation of transcription, DNA-templated Source: CACAO
  • quorum sensing Source: UniProtKB-KW
  • regulation of elastin catabolic process Source: PseudoCAP
  • regulation of gene expression Source: CACAO
  • regulation of transcription, DNA-templated Source: PseudoCAP
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Quorum sensing, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional activator protein LasR
Gene namesi
Name:lasR
Ordered Locus Names:PA1430
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA1430.

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1075207.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001841601 – 239Transcriptional activator protein LasRAdd BLAST239

Proteomic databases

PaxDbiP25084.
PRIDEiP25084.

Expressioni

Gene expression databases

CollecTFiEXPREG_000009b0.

Interactioni

Protein-protein interaction databases

STRINGi208964.PA1430.

Chemistry databases

BindingDBiP25084.

Structurei

Secondary structure

1239
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 12Combined sources5
Helixi15 – 29Combined sources15
Beta strandi33 – 40Combined sources8
Helixi47 – 49Combined sources3
Beta strandi50 – 54Combined sources5
Helixi58 – 66Combined sources9
Helixi69 – 71Combined sources3
Helixi74 – 81Combined sources8
Helixi90 – 92Combined sources3
Helixi96 – 107Combined sources12
Beta strandi112 – 119Combined sources8
Beta strandi125 – 131Combined sources7
Helixi137 – 145Combined sources9
Helixi148 – 167Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2UV0X-ray1.80E/F/G/H1-173[»]
3IX3X-ray1.40A/B1-173[»]
3IX4X-ray1.80A/B/C/D/E/F/G/H1-173[»]
3IX8X-ray1.80A/B/C/D1-173[»]
3JPUX-ray2.30A/B/C/D/E1-173[»]
4NG2X-ray2.41A/B/C/D1-170[»]
ProteinModelPortaliP25084.
SMRiP25084.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25084.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini170 – 235HTH luxR-typePROSITE-ProRule annotationAdd BLAST66

Sequence similaritiesi

Contains 1 HTH luxR-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105VJY. Bacteria.
COG2771. LUCA.
HOGENOMiHOG000111053.
InParanoidiP25084.
KOiK18304.
OMAiHAWHSIT.
PhylomeDBiP25084.

Family and domain databases

CDDicd06170. LuxR_C_like. 1 hit.
Gene3Di1.10.10.10. 1 hit.
3.30.450.80. 1 hit.
InterProiIPR016032. Sig_transdc_resp-reg_C-effctor.
IPR005143. TF_LuxR_autoind-bd_dom.
IPR000792. Tscrpt_reg_LuxR_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF03472. Autoind_bind. 1 hit.
PF00196. GerE. 1 hit.
[Graphical view]
PRINTSiPR00038. HTHLUXR.
SMARTiSM00421. HTH_LUXR. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
SSF75516. SSF75516. 1 hit.
PROSITEiPS00622. HTH_LUXR_1. 1 hit.
PS50043. HTH_LUXR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25084-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALVDGFLEL ERSSGKLEWS AILQKMASDL GFSKILFGLL PKDSQDYENA
60 70 80 90 100
FIVGNYPAAW REHYDRAGYA RVDPTVSHCT QSVLPIFWEP SIYQTRKQHE
110 120 130 140 150
FFEEASAAGL VYGLTMPLHG ARGELGALSL SVEAENRAEA NRFMESVLPT
160 170 180 190 200
LWMLKDYALQ SGAGLAFEHP VSKPVVLTSR EKEVLQWCAI GKTSWEISVI
210 220 230
CNCSEANVNF HMGNIRRKFG VTSRRVAAIM AVNLGLITL
Length:239
Mass (Da):26,619
Last modified:May 1, 1992 - v1
Checksum:i28B6E06E9187924A
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti144M → I in strain: IFO 3455 and PA103. 1
Natural varianti180R → W in strain: PA103. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59425 Genomic DNA. Translation: AAA25874.1.
D30813 Genomic DNA. Translation: BAA06490.1.
D30812 Genomic DNA. Translation: BAA06489.1.
AE004091 Genomic DNA. Translation: AAG04819.1.
PIRiA43660.
S53692.
S53693.
RefSeqiNP_250121.1. NC_002516.2.
WP_003082999.1. NZ_ASJY01000215.1.

Genome annotation databases

EnsemblBacteriaiAAG04819; AAG04819; PA1430.
GeneIDi881789.
KEGGipae:PA1430.
PATRICi19837188. VBIPseAer58763_1480.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59425 Genomic DNA. Translation: AAA25874.1.
D30813 Genomic DNA. Translation: BAA06490.1.
D30812 Genomic DNA. Translation: BAA06489.1.
AE004091 Genomic DNA. Translation: AAG04819.1.
PIRiA43660.
S53692.
S53693.
RefSeqiNP_250121.1. NC_002516.2.
WP_003082999.1. NZ_ASJY01000215.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2UV0X-ray1.80E/F/G/H1-173[»]
3IX3X-ray1.40A/B1-173[»]
3IX4X-ray1.80A/B/C/D/E/F/G/H1-173[»]
3IX8X-ray1.80A/B/C/D1-173[»]
3JPUX-ray2.30A/B/C/D/E1-173[»]
4NG2X-ray2.41A/B/C/D1-170[»]
ProteinModelPortaliP25084.
SMRiP25084.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA1430.

Chemistry databases

BindingDBiP25084.
ChEMBLiCHEMBL1075207.

Proteomic databases

PaxDbiP25084.
PRIDEiP25084.

Protocols and materials databases

DNASUi881789.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG04819; AAG04819; PA1430.
GeneIDi881789.
KEGGipae:PA1430.
PATRICi19837188. VBIPseAer58763_1480.

Organism-specific databases

PseudoCAPiPA1430.

Phylogenomic databases

eggNOGiENOG4105VJY. Bacteria.
COG2771. LUCA.
HOGENOMiHOG000111053.
InParanoidiP25084.
KOiK18304.
OMAiHAWHSIT.
PhylomeDBiP25084.

Miscellaneous databases

EvolutionaryTraceiP25084.
PROiP25084.

Gene expression databases

CollecTFiEXPREG_000009b0.

Family and domain databases

CDDicd06170. LuxR_C_like. 1 hit.
Gene3Di1.10.10.10. 1 hit.
3.30.450.80. 1 hit.
InterProiIPR016032. Sig_transdc_resp-reg_C-effctor.
IPR005143. TF_LuxR_autoind-bd_dom.
IPR000792. Tscrpt_reg_LuxR_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF03472. Autoind_bind. 1 hit.
PF00196. GerE. 1 hit.
[Graphical view]
PRINTSiPR00038. HTHLUXR.
SMARTiSM00421. HTH_LUXR. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
SSF75516. SSF75516. 1 hit.
PROSITEiPS00622. HTH_LUXR_1. 1 hit.
PS50043. HTH_LUXR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLASR_PSEAE
AccessioniPrimary (citable) accession number: P25084
Secondary accession number(s): Q51475, Q51476
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: November 2, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

LasR in strain PA103 is not active, this is probably due to the change in position 180 of the sequence.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.