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Protein

Transcriptional activator protein LasR

Gene

lasR

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator of elastase structural gene (LasB). Binds to the PAI autoinducer.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi194 – 21320H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • transcription factor activity, sequence-specific DNA binding Source: PseudoCAP

GO - Biological processi

  • pathogenesis Source: PseudoCAP
  • positive regulation of elastin biosynthetic process Source: PseudoCAP
  • positive regulation of transcription, DNA-templated Source: CACAO
  • quorum sensing Source: UniProtKB-KW
  • regulation of elastin catabolic process Source: PseudoCAP
  • regulation of gene expression Source: CACAO
  • regulation of transcription, DNA-templated Source: PseudoCAP
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Quorum sensing, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional activator protein LasR
Gene namesi
Name:lasR
Ordered Locus Names:PA1430
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA1430.

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL1075207.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 239239Transcriptional activator protein LasRPRO_0000184160Add
BLAST

Proteomic databases

PaxDbiP25084.

Expressioni

Gene expression databases

CollecTFiEXPREG_000009b0.

Interactioni

Protein-protein interaction databases

STRINGi208964.PA1430.

Chemistry

BindingDBiP25084.

Structurei

Secondary structure

1
239
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi8 – 125Combined sources
Helixi15 – 2915Combined sources
Beta strandi33 – 408Combined sources
Helixi47 – 493Combined sources
Beta strandi50 – 545Combined sources
Helixi58 – 669Combined sources
Helixi69 – 713Combined sources
Helixi74 – 818Combined sources
Helixi90 – 923Combined sources
Helixi96 – 10712Combined sources
Beta strandi112 – 1198Combined sources
Beta strandi125 – 1317Combined sources
Helixi137 – 1459Combined sources
Helixi148 – 16720Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2UV0X-ray1.80E/F/G/H1-173[»]
3IX3X-ray1.40A/B1-173[»]
3IX4X-ray1.80A/B/C/D/E/F/G/H1-173[»]
3IX8X-ray1.80A/B/C/D1-173[»]
3JPUX-ray2.30A/B/C/D/E1-173[»]
4NG2X-ray2.41A/B/C/D1-170[»]
ProteinModelPortaliP25084.
SMRiP25084. Positions 1-237.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25084.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini170 – 23566HTH luxR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HTH luxR-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105VJY. Bacteria.
COG2771. LUCA.
HOGENOMiHOG000111053.
InParanoidiP25084.
KOiK18304.
OMAiMAPLLNA.
OrthoDBiEOG69D3BB.
PhylomeDBiP25084.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.30.450.80. 1 hit.
InterProiIPR016032. Sig_transdc_resp-reg_C-effctor.
IPR005143. TF_LuxR_autoind-bd_dom.
IPR000792. Tscrpt_reg_LuxR_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF03472. Autoind_bind. 1 hit.
PF00196. GerE. 1 hit.
[Graphical view]
PRINTSiPR00038. HTHLUXR.
SMARTiSM00421. HTH_LUXR. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
SSF75516. SSF75516. 1 hit.
PROSITEiPS00622. HTH_LUXR_1. 1 hit.
PS50043. HTH_LUXR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25084-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALVDGFLEL ERSSGKLEWS AILQKMASDL GFSKILFGLL PKDSQDYENA
60 70 80 90 100
FIVGNYPAAW REHYDRAGYA RVDPTVSHCT QSVLPIFWEP SIYQTRKQHE
110 120 130 140 150
FFEEASAAGL VYGLTMPLHG ARGELGALSL SVEAENRAEA NRFMESVLPT
160 170 180 190 200
LWMLKDYALQ SGAGLAFEHP VSKPVVLTSR EKEVLQWCAI GKTSWEISVI
210 220 230
CNCSEANVNF HMGNIRRKFG VTSRRVAAIM AVNLGLITL
Length:239
Mass (Da):26,619
Last modified:May 1, 1992 - v1
Checksum:i28B6E06E9187924A
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti144 – 1441M → I in strain: IFO 3455 and PA103.
Natural varianti180 – 1801R → W in strain: PA103.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59425 Genomic DNA. Translation: AAA25874.1.
D30813 Genomic DNA. Translation: BAA06490.1.
D30812 Genomic DNA. Translation: BAA06489.1.
AE004091 Genomic DNA. Translation: AAG04819.1.
PIRiA43660.
S53692.
S53693.
RefSeqiNP_250121.1. NC_002516.2.
WP_003082999.1. NZ_ASJY01000215.1.

Genome annotation databases

EnsemblBacteriaiAAG04819; AAG04819; PA1430.
GeneIDi881789.
KEGGipae:PA1430.
PATRICi19837188. VBIPseAer58763_1480.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59425 Genomic DNA. Translation: AAA25874.1.
D30813 Genomic DNA. Translation: BAA06490.1.
D30812 Genomic DNA. Translation: BAA06489.1.
AE004091 Genomic DNA. Translation: AAG04819.1.
PIRiA43660.
S53692.
S53693.
RefSeqiNP_250121.1. NC_002516.2.
WP_003082999.1. NZ_ASJY01000215.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2UV0X-ray1.80E/F/G/H1-173[»]
3IX3X-ray1.40A/B1-173[»]
3IX4X-ray1.80A/B/C/D/E/F/G/H1-173[»]
3IX8X-ray1.80A/B/C/D1-173[»]
3JPUX-ray2.30A/B/C/D/E1-173[»]
4NG2X-ray2.41A/B/C/D1-170[»]
ProteinModelPortaliP25084.
SMRiP25084. Positions 1-237.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA1430.

Chemistry

BindingDBiP25084.
ChEMBLiCHEMBL1075207.

Proteomic databases

PaxDbiP25084.

Protocols and materials databases

DNASUi881789.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG04819; AAG04819; PA1430.
GeneIDi881789.
KEGGipae:PA1430.
PATRICi19837188. VBIPseAer58763_1480.

Organism-specific databases

PseudoCAPiPA1430.

Phylogenomic databases

eggNOGiENOG4105VJY. Bacteria.
COG2771. LUCA.
HOGENOMiHOG000111053.
InParanoidiP25084.
KOiK18304.
OMAiMAPLLNA.
OrthoDBiEOG69D3BB.
PhylomeDBiP25084.

Miscellaneous databases

EvolutionaryTraceiP25084.
PROiP25084.

Gene expression databases

CollecTFiEXPREG_000009b0.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.30.450.80. 1 hit.
InterProiIPR016032. Sig_transdc_resp-reg_C-effctor.
IPR005143. TF_LuxR_autoind-bd_dom.
IPR000792. Tscrpt_reg_LuxR_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF03472. Autoind_bind. 1 hit.
PF00196. GerE. 1 hit.
[Graphical view]
PRINTSiPR00038. HTHLUXR.
SMARTiSM00421. HTH_LUXR. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
SSF75516. SSF75516. 1 hit.
PROSITEiPS00622. HTH_LUXR_1. 1 hit.
PS50043. HTH_LUXR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of the Pseudomonas aeruginosa lasR gene, a transcriptional activator of elastase expression."
    Gambello M.J., Iglewski B.H.
    J. Bacteriol. 173:3000-3009(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  2. "Intracellular receptor-type transcription factor, LasR, contains a highly conserved amphipathic region which precedes the putative helix-turn-helix DNA binding motif."
    Fukushima J., Ishiwata T., Kurata M., You Z., Okuda K.
    Nucleic Acids Res. 22:3706-3707(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 29260 / PA103 and NBRC 13130 / JCM 20134.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiLASR_PSEAE
AccessioniPrimary (citable) accession number: P25084
Secondary accession number(s): Q51475, Q51476
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: June 8, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

LasR in strain PA103 is not active, this is probably due to the change in position 180 of the sequence.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.