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Protein

Mannose-6-phosphate isomerase

Gene

manA

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the conversion of glucose to GDP-L-fucose, which can be converted to L-fucose, a capsular polysaccharide.

Catalytic activityi

D-mannose 6-phosphate = D-fructose 6-phosphate.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi97ZincBy similarity1
Metal bindingi99ZincBy similarity1
Metal bindingi134ZincBy similarity1
Metal bindingi255ZincBy similarity1
Active sitei274By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi5.3.1.8. 5542.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannose-6-phosphate isomerase (EC:5.3.1.8)
Alternative name(s):
Phosphohexomutase
Phosphomannose isomerase
Short name:
PMI
Gene namesi
Name:manA
Synonyms:pmi
Ordered Locus Names:STM1467
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001942311 – 391Mannose-6-phosphate isomeraseAdd BLAST391

Proteomic databases

PaxDbiP25081.
PRIDEiP25081.

Interactioni

Protein-protein interaction databases

STRINGi99287.STM1467.

Structurei

Secondary structure

1391
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi1 – 3Combined sources3
Beta strandi7 – 9Combined sources3
Beta strandi14 – 17Combined sources4
Helixi18 – 23Combined sources6
Beta strandi35 – 39Combined sources5
Beta strandi47 – 49Combined sources3
Beta strandi55 – 57Combined sources3
Helixi58 – 64Combined sources7
Helixi66 – 70Combined sources5
Helixi72 – 77Combined sources6
Beta strandi84 – 92Combined sources9
Helixi102 – 114Combined sources13
Beta strandi134 – 140Combined sources7
Beta strandi142 – 148Combined sources7
Helixi151 – 158Combined sources8
Helixi159 – 164Combined sources6
Helixi166 – 173Combined sources8
Helixi177 – 188Combined sources12
Helixi192 – 208Combined sources17
Helixi214 – 223Combined sources10
Helixi228 – 231Combined sources4
Helixi232 – 235Combined sources4
Beta strandi236 – 241Combined sources6
Beta strandi246 – 249Combined sources4
Beta strandi255 – 266Combined sources12
Beta strandi276 – 280Combined sources5
Helixi284 – 289Combined sources6
Helixi298 – 300Combined sources3
Beta strandi306 – 308Combined sources3
Beta strandi311 – 313Combined sources3
Beta strandi317 – 320Combined sources4
Beta strandi322 – 327Combined sources6
Beta strandi333 – 335Combined sources3
Beta strandi341 – 354Combined sources14
Beta strandi357 – 361Combined sources5
Beta strandi366 – 369Combined sources4
Helixi371 – 373Combined sources3
Beta strandi376 – 388Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WFPX-ray1.67A1-391[»]
3H1MX-ray2.50A1-391[»]
3H1WX-ray1.94A1-391[»]
3H1YX-ray2.04A1-391[»]
ProteinModelPortaliP25081.
SMRiP25081.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25081.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105EIP. Bacteria.
COG1482. LUCA.
HOGENOMiHOG000241278.
KOiK01809.
OMAiQGEPWDT.
PhylomeDBiP25081.

Family and domain databases

Gene3Di2.60.120.10. 3 hits.
InterProiIPR001250. Man6P_Isoase-1.
IPR016305. Mannose-6-P_Isomerase.
IPR018050. Pmannose_isomerase-type1_CS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR10309. PTHR10309. 1 hit.
PfamiPF01238. PMI_typeI. 1 hit.
[Graphical view]
PIRSFiPIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTSiPR00714. MAN6PISMRASE.
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR00218. manA. 1 hit.
PROSITEiPS00965. PMI_I_1. 1 hit.
PS00966. PMI_I_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25081-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQKLINSVQN YAWGSKTALT ELYGIANPQQ QPMAELWMGA HPKSSSRITT
60 70 80 90 100
ANGETVSLRD AIEKNKTAML GEAVANRFGE LPFLFKVLCA AQPLSIQVHP
110 120 130 140 150
NKRNSEIGFA KENAAGIPMD AAERNYKDPN HKPELVFALT PFLAMNAFRE
160 170 180 190 200
FSDIVSLLQP VAGAHSAIAH FLQVPNAERL SQLFASLLNM QGEEKSRALA
210 220 230 240 250
VLKAALNSQQ GEPWQTIRVI SEYYPDDSGL FSPLLLNVVK LNPGEAMFLF
260 270 280 290 300
AETPHAYLQG VALEVMANSD NVLRAGLTPK YIDIPELVAN VKFEPKPAGE
310 320 330 340 350
LLTAPVKSGA ELDFPIPVDD FAFSLHDLAL QETSIGQHSA AILFCVEGEA
360 370 380 390
VLRKDEQRLV LKPGESAFIG ADESPVNASG TGRLARVYNK L
Length:391
Mass (Da):42,591
Last modified:January 23, 2002 - v2
Checksum:i61E7355146DBEF5A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti92Q → K in CAA40399 (PubMed:1879695).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57117 Genomic DNA. Translation: CAA40399.1.
AE006468 Genomic DNA. Translation: AAL20387.1.
PIRiJQ1192.
RefSeqiNP_460428.1. NC_003197.1.
WP_001170627.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL20387; AAL20387; STM1467.
GeneIDi1252985.
KEGGistm:STM1467.
PATRICi32381417. VBISalEnt20916_1549.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57117 Genomic DNA. Translation: CAA40399.1.
AE006468 Genomic DNA. Translation: AAL20387.1.
PIRiJQ1192.
RefSeqiNP_460428.1. NC_003197.1.
WP_001170627.1. NC_003197.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WFPX-ray1.67A1-391[»]
3H1MX-ray2.50A1-391[»]
3H1WX-ray1.94A1-391[»]
3H1YX-ray2.04A1-391[»]
ProteinModelPortaliP25081.
SMRiP25081.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM1467.

Proteomic databases

PaxDbiP25081.
PRIDEiP25081.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL20387; AAL20387; STM1467.
GeneIDi1252985.
KEGGistm:STM1467.
PATRICi32381417. VBISalEnt20916_1549.

Phylogenomic databases

eggNOGiENOG4105EIP. Bacteria.
COG1482. LUCA.
HOGENOMiHOG000241278.
KOiK01809.
OMAiQGEPWDT.
PhylomeDBiP25081.

Enzyme and pathway databases

BRENDAi5.3.1.8. 5542.

Miscellaneous databases

EvolutionaryTraceiP25081.

Family and domain databases

Gene3Di2.60.120.10. 3 hits.
InterProiIPR001250. Man6P_Isoase-1.
IPR016305. Mannose-6-P_Isomerase.
IPR018050. Pmannose_isomerase-type1_CS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR10309. PTHR10309. 1 hit.
PfamiPF01238. PMI_typeI. 1 hit.
[Graphical view]
PIRSFiPIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTSiPR00714. MAN6PISMRASE.
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR00218. manA. 1 hit.
PROSITEiPS00965. PMI_I_1. 1 hit.
PS00966. PMI_I_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMANA_SALTY
AccessioniPrimary (citable) accession number: P25081
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2002
Last modified: November 30, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.