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P25081 (MANA_SALTY) Reviewed, UniProtKB/Swiss-Prot

Last modified January 22, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mannose-6-phosphate isomerase

EC=5.3.1.8
Alternative name(s):
Phosphohexomutase
Phosphomannose isomerase
Short name=PMI
Gene names
Name:manA
Synonyms:pmi
Ordered Locus Names:STM1467
OrganismSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]
Taxonomic identifier99287 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

Protein attributes

Sequence length391 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the conversion of glucose to GDP-L-fucose, which can be converted to L-fucose, a capsular polysaccharide.

Catalytic activity

D-mannose 6-phosphate = D-fructose 6-phosphate.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subcellular location

Cytoplasm Probable.

Sequence similarities

Belongs to the mannose-6-phosphate isomerase type 1 family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandMetal-binding
Zinc
   Molecular functionIsomerase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processGDP-mannose biosynthetic process

Inferred from electronic annotation. Source: InterPro

carbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmannose-6-phosphate isomerase activity

Inferred from electronic annotation. Source: UniProtKB-EC

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 391391Mannose-6-phosphate isomerase
PRO_0000194231

Sites

Active site2741 By similarity
Metal binding971Zinc By similarity
Metal binding991Zinc By similarity
Metal binding1341Zinc By similarity
Metal binding2551Zinc By similarity

Experimental info

Sequence conflict921Q → K in CAA40399. Ref.1

Secondary structure

....................................................................... 391
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P25081 [UniParc].

Last modified January 23, 2002. Version 2.
Checksum: 61E7355146DBEF5A

FASTA39142,591
        10         20         30         40         50         60 
MQKLINSVQN YAWGSKTALT ELYGIANPQQ QPMAELWMGA HPKSSSRITT ANGETVSLRD 

        70         80         90        100        110        120 
AIEKNKTAML GEAVANRFGE LPFLFKVLCA AQPLSIQVHP NKRNSEIGFA KENAAGIPMD 

       130        140        150        160        170        180 
AAERNYKDPN HKPELVFALT PFLAMNAFRE FSDIVSLLQP VAGAHSAIAH FLQVPNAERL 

       190        200        210        220        230        240 
SQLFASLLNM QGEEKSRALA VLKAALNSQQ GEPWQTIRVI SEYYPDDSGL FSPLLLNVVK 

       250        260        270        280        290        300 
LNPGEAMFLF AETPHAYLQG VALEVMANSD NVLRAGLTPK YIDIPELVAN VKFEPKPAGE 

       310        320        330        340        350        360 
LLTAPVKSGA ELDFPIPVDD FAFSLHDLAL QETSIGQHSA AILFCVEGEA VLRKDEQRLV 

       370        380        390 
LKPGESAFIG ADESPVNASG TGRLARVYNK L 

« Hide

References

« Hide 'large scale' references
[1]"Sequence of the phosphomannose isomerase-encoding gene of Salmonella typhimurium."
Collins L.V., Hackett J.
Gene 103:135-136(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: C5.
[2]"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2."
McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. expand/collapse author list , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K.
Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LT2 / SGSC1412 / ATCC 700720.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X57117 Genomic DNA. Translation: CAA40399.1.
AE006468 Genomic DNA. Translation: AAL20387.1.
PIRJQ1192.
RefSeqNP_460428.1. NC_003197.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2WFPX-ray1.67A1-391[»]
3H1MX-ray2.50A1-391[»]
3H1WX-ray1.94A1-391[»]
3H1YX-ray2.04A1-391[»]
ProteinModelPortalP25081.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING99287.STM1467.

Proteomic databases

PaxDbP25081.
PRIDEP25081.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL20387; AAL20387; STM1467.
GeneID1252985.
KEGGstm:STM1467.
PATRIC32381417. VBISalEnt20916_1549.

Phylogenomic databases

eggNOGCOG1482.
HOGENOMHOG000241278.
KOK01809.
OMAFKVLCAD.
OrthoDBEOG6130B2.
ProtClustDBPRK15131.

Enzyme and pathway databases

BioCycSENT99287:GCTI-1477-MONOMER.

Family and domain databases

Gene3D2.60.120.10. 3 hits.
InterProIPR001250. Man6P_Isoase-1.
IPR016305. Mannose-6-P_Isomerase.
IPR018050. Pmannose_isomerase-type1_CS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERPTHR10309. PTHR10309. 1 hit.
PfamPF01238. PMI_typeI. 1 hit.
[Graphical view]
PIRSFPIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTSPR00714. MAN6PISMRASE.
SUPFAMSSF51182. SSF51182. 1 hit.
TIGRFAMsTIGR00218. manA. 1 hit.
PROSITEPS00965. PMI_I_1. 1 hit.
PS00966. PMI_I_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP25081.

Entry information

Entry nameMANA_SALTY
AccessionPrimary (citable) accession number: P25081
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2002
Last modified: January 22, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references