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P25080 (HUTU_PSEPU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Urocanate hydratase
Short name=Urocanase
EC=4.2.1.49
Alternative name(s):
Imidazolonepropionate hydrolase
Gene names
Name:hutU
OrganismPseudomonas putida (Arthrobacter siderocapsulatus)
Taxonomic identifier303 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas ›

Protein attributes

Sequence length557 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate = urocanate + H2O. HAMAP-Rule MF_00577

Cofactor

Binds 1 NAD per subunit.

Pathway

Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 2/3. HAMAP-Rule MF_00577

Subunit structure

Homodimer.

Subcellular location

Cytoplasm HAMAP-Rule MF_00577.

Sequence similarities

Belongs to the urocanase family.

Mass spectrometry

Molecular mass is 60686±15 Da from positions 2 - 557. Determined by ESI. Ref.3

Ontologies

Keywords
   Biological processHistidine metabolism
   Cellular componentCytoplasm
   LigandNAD
   Molecular functionLyase
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Biological_processhistidine catabolic process to glutamate and formamide

Inferred from electronic annotation. Source: UniProtKB-UniPathway

histidine catabolic process to glutamate and formate

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionurocanate hydratase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1
Chain2 – 557556Urocanate hydratase HAMAP-Rule MF_00577
PRO_0000207344

Sites

Active site4111

Experimental info

Mutagenesis641C → A: No loss of activity.
Mutagenesis1921C → A: No loss of activity.
Mutagenesis1981C → A: No loss of activity.
Mutagenesis3551C → A: Minor loss in activity.
Mutagenesis4111C → A: Loss of activity.
Mutagenesis5441C → A: No loss of activity.
Sequence conflict164 – 1674TVKA → SLKG in AAA50311. Ref.2

Secondary structure

1 ........................................................................................... 557
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P25080 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 5AFD1E3307F34434

FASTA55760,804
        10         20         30         40         50         60 
MTDNNKYRDV EIRAPRGNKL TAKSWLTEAP LRMLMNNLDP QVAENPKELV VYGGIGRAAR 

        70         80         90        100        110        120 
NWECYDKIVE TLTRLEDDET LLVQSGKPVG VFKTHSNAPR VLIANSNLVP HWANWEHFNE 

       130        140        150        160        170        180 
LDAKGLAMYG QMTAGSWIYI GSQGIVQGTY ETFVEAGRQH YGGTVKAKWV LTAGLGGMGG 

       190        200        210        220        230        240 
AQPLAATLAG ACSLNIECQQ SRIDFRLETR YVDEQATDLD DALVRIAKYT AEGKAISIAL 

       250        260        270        280        290        300 
HGNAAEILPE LVKRGVRPDM VTDQTSAHDP LNGYLPAGWT WEQYRDRAQT EPAAVVKAAK 

       310        320        330        340        350        360 
QSMAVHVQAM LDFQKQGVPT FDYGNNIRQM AKEEGVANAF DFPGFVPAYI RPLFCRGVGP 

       370        380        390        400        410        420 
FRWAALSGEA EDIYKTDAKV KELIPDDAHL HRWLDMARER ISFQGLPARI CWVGLGLRAK 

       430        440        450        460        470        480 
LGLAFNEMVR SGELSAPVVI GRDHLDSGSV SSPNRETEAM RDGSDAVSDW PLLNALLNTA 

       490        500        510        520        530        540 
GGATWVSLHH GGGVGMGFSQ HSGMVIVCDG TDEAAERIAR VLTNDPGTGV MRHADAGYDI 

       550 
AIDCAKEQGL DLPMITG

« Hide

References

[1]"Cloning and sequencing the urocanase gene (hutU) from Pseudomonas putida."
Fessemaier M., Frank R., Retey J., Schubert C.
FEBS Lett. 286:55-57(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-23.
Strain: NIC II.
[2]Allison S.L., Phillips A.T.
Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 12633 / DSM 291 / JCM 13063 / NCIMB 9494 / NCTC 10936 / VKM B-2187 / Stanier 90.
[3]"Cloning, expression and mutational analysis of the urocanase gene (hutU) from Pseudomonas putida."
Lenz M., Retey J.
Eur. J. Biochem. 217:429-434(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS, MASS SPECTROMETRY.
Strain: NIC II.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X58483 Genomic DNA. Translation: CAA41392.1.
M33923 Genomic DNA. Translation: AAA50311.1.
PIRDWPSUP. S17184.
T45008.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1UWKX-ray1.19A/B1-557[»]
1UWLX-ray1.76A/B1-557[»]
1W1UX-ray2.23A/B2-556[»]
2V7GX-ray2.00A/B/C/D2-556[»]
ProteinModelPortalP25080.
SMRP25080. Positions 5-554.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-11616.
UniPathwayUPA00379; UER00550.

Family and domain databases

HAMAPMF_00577. HutU.
InterProIPR000193. Urocanase.
IPR023636. Urocanase_CS.
IPR023637. Urocanase_dom.
[Graphical view]
PANTHERPTHR32312. PTHR32312. 1 hit.
PfamPF01175. Urocanase. 1 hit.
[Graphical view]
PIRSFPIRSF001423. Urocanate_hydrat. 1 hit.
SUPFAMSSF111326. Urocanase. 1 hit.
TIGRFAMsTIGR01228. hutU. 1 hit.
PROSITEPS01233. UROCANASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP25080.

Entry information

Entry nameHUTU_PSEPU
AccessionPrimary (citable) accession number: P25080
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2007
Last modified: April 3, 2013
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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