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Protein

Urocanate hydratase

Gene

hutU

Organism
Pseudomonas putida (Arthrobacter siderocapsulatus)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate = urocanate + H2O.

Cofactori

NAD+Note: Binds 1 NAD+ per subunit.

Pathwayi: L-histidine degradation into L-glutamate

This protein is involved in step 2 of the subpathway that synthesizes N-formimidoyl-L-glutamate from L-histidine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Histidine ammonia-lyase (DW66_2585), Histidine ammonia-lyase (hutH), Histidine ammonia-lyase (PU99_10260), Histidine ammonia-lyase (hutH), Histidine ammonia-lyase (HB13667_15065), Histidine ammonia-lyase (hutH), Histidine ammonia-lyase (hutH), Histidine ammonia-lyase (HB4184_14930), Histidine ammonia-lyase (hutH), Histidine ammonia-lyase (hutH), Histidine ammonia-lyase (hutH), Histidine ammonia-lyase (HA62_16700), Histidine ammonia-lyase (hutH)
  2. Urocanate hydratase (hutU), Urocanate hydratase (hutU), Urocanate hydratase (hutU), Urocanate hydratase (hutU), Urocanate hydratase (hutU), Urocanate hydratase (hutU), Urocanate hydratase (hutU), Urocanate hydratase (hutU), Urocanate hydratase (hutU)
  3. Imidazolonepropionase (hutI), Imidazolonepropionase (hutI), Imidazolonepropionase (hutI), Imidazolonepropionase (hutI), Imidazolonepropionase (hutI), Imidazolonepropionase (hutI), Imidazolonepropionase (hutI), Imidazolonepropionase (hutI)
This subpathway is part of the pathway L-histidine degradation into L-glutamate, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N-formimidoyl-L-glutamate from L-histidine, the pathway L-histidine degradation into L-glutamate and in Amino-acid degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei411 – 4111

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Histidine metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-11616.
BRENDAi4.2.1.49. 5092.
UniPathwayiUPA00379; UER00550.

Names & Taxonomyi

Protein namesi
Recommended name:
Urocanate hydratase (EC:4.2.1.49)
Short name:
Urocanase
Alternative name(s):
Imidazolonepropionate hydrolase
Gene namesi
Name:hutU
OrganismiPseudomonas putida (Arthrobacter siderocapsulatus)
Taxonomic identifieri303 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi64 – 641C → A: No loss of activity. 1 Publication
Mutagenesisi192 – 1921C → A: No loss of activity. 1 Publication
Mutagenesisi198 – 1981C → A: No loss of activity. 1 Publication
Mutagenesisi355 – 3551C → A: Minor loss in activity. 1 Publication
Mutagenesisi411 – 4111C → A: Loss of activity. 1 Publication
Mutagenesisi544 – 5441C → A: No loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 557556Urocanate hydratasePRO_0000207344Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi160488.PP_5033.

Structurei

Secondary structure

1
557
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi21 – 244Combined sources
Helixi25 – 3713Combined sources
Turni40 – 423Combined sources
Helixi46 – 483Combined sources
Beta strandi50 – 523Combined sources
Turni53 – 553Combined sources
Beta strandi56 – 616Combined sources
Helixi62 – 7413Combined sources
Beta strandi79 – 846Combined sources
Beta strandi87 – 937Combined sources
Beta strandi100 – 1067Combined sources
Helixi110 – 1123Combined sources
Helixi115 – 1239Combined sources
Turni131 – 1377Combined sources
Helixi143 – 16018Combined sources
Beta strandi161 – 1633Combined sources
Beta strandi169 – 1735Combined sources
Turni177 – 1804Combined sources
Helixi181 – 1888Combined sources
Beta strandi192 – 1987Combined sources
Helixi200 – 2089Combined sources
Helixi219 – 23113Combined sources
Beta strandi237 – 2426Combined sources
Helixi244 – 25411Combined sources
Beta strandi259 – 2613Combined sources
Turni270 – 2723Combined sources
Helixi281 – 29010Combined sources
Helixi292 – 31524Combined sources
Helixi327 – 3337Combined sources
Helixi339 – 3413Combined sources
Helixi345 – 3484Combined sources
Helixi351 – 3544Combined sources
Turni355 – 3573Combined sources
Beta strandi361 – 3655Combined sources
Helixi370 – 38314Combined sources
Helixi388 – 40013Combined sources
Beta strandi408 – 4103Combined sources
Helixi417 – 43014Combined sources
Beta strandi433 – 4364Combined sources
Beta strandi438 – 4425Combined sources
Beta strandi449 – 4513Combined sources
Turni453 – 4597Combined sources
Helixi469 – 48113Combined sources
Beta strandi484 – 4918Combined sources
Turni492 – 4943Combined sources
Beta strandi500 – 5089Combined sources
Helixi512 – 53524Combined sources
Helixi539 – 5479Combined sources
Turni553 – 5553Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1UWKX-ray1.19A/B1-557[»]
1UWLX-ray1.76A/B1-557[»]
1W1UX-ray2.23A/B2-557[»]
2V7GX-ray2.00A/B/C/D2-557[»]
ProteinModelPortaliP25080.
SMRiP25080. Positions 5-554.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25080.

Family & Domainsi

Sequence similaritiesi

Belongs to the urocanase family.Curated

Phylogenomic databases

eggNOGiENOG4105CGP. Bacteria.
COG2987. LUCA.

Family and domain databases

HAMAPiMF_00577. HutU.
InterProiIPR023637. Urocanase.
IPR023636. Urocanase_CS.
[Graphical view]
PfamiPF01175. Urocanase. 1 hit.
[Graphical view]
PIRSFiPIRSF001423. Urocanate_hydrat. 1 hit.
SUPFAMiSSF111326. SSF111326. 1 hit.
TIGRFAMsiTIGR01228. hutU. 1 hit.
PROSITEiPS01233. UROCANASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25080-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDNNKYRDV EIRAPRGNKL TAKSWLTEAP LRMLMNNLDP QVAENPKELV
60 70 80 90 100
VYGGIGRAAR NWECYDKIVE TLTRLEDDET LLVQSGKPVG VFKTHSNAPR
110 120 130 140 150
VLIANSNLVP HWANWEHFNE LDAKGLAMYG QMTAGSWIYI GSQGIVQGTY
160 170 180 190 200
ETFVEAGRQH YGGTVKAKWV LTAGLGGMGG AQPLAATLAG ACSLNIECQQ
210 220 230 240 250
SRIDFRLETR YVDEQATDLD DALVRIAKYT AEGKAISIAL HGNAAEILPE
260 270 280 290 300
LVKRGVRPDM VTDQTSAHDP LNGYLPAGWT WEQYRDRAQT EPAAVVKAAK
310 320 330 340 350
QSMAVHVQAM LDFQKQGVPT FDYGNNIRQM AKEEGVANAF DFPGFVPAYI
360 370 380 390 400
RPLFCRGVGP FRWAALSGEA EDIYKTDAKV KELIPDDAHL HRWLDMARER
410 420 430 440 450
ISFQGLPARI CWVGLGLRAK LGLAFNEMVR SGELSAPVVI GRDHLDSGSV
460 470 480 490 500
SSPNRETEAM RDGSDAVSDW PLLNALLNTA GGATWVSLHH GGGVGMGFSQ
510 520 530 540 550
HSGMVIVCDG TDEAAERIAR VLTNDPGTGV MRHADAGYDI AIDCAKEQGL

DLPMITG
Length:557
Mass (Da):60,804
Last modified:January 23, 2007 - v2
Checksum:i5AFD1E3307F34434
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti164 – 1674TVKA → SLKG in AAA50311 (Ref. 2) Curated

Mass spectrometryi

Molecular mass is 60686±15 Da from positions 2 - 557. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58483 Genomic DNA. Translation: CAA41392.1.
M33923 Genomic DNA. Translation: AAA50311.1.
PIRiS17184. DWPSUP.
T45008.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58483 Genomic DNA. Translation: CAA41392.1.
M33923 Genomic DNA. Translation: AAA50311.1.
PIRiS17184. DWPSUP.
T45008.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1UWKX-ray1.19A/B1-557[»]
1UWLX-ray1.76A/B1-557[»]
1W1UX-ray2.23A/B2-557[»]
2V7GX-ray2.00A/B/C/D2-557[»]
ProteinModelPortaliP25080.
SMRiP25080. Positions 5-554.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi160488.PP_5033.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105CGP. Bacteria.
COG2987. LUCA.

Enzyme and pathway databases

UniPathwayiUPA00379; UER00550.
BioCyciMetaCyc:MONOMER-11616.
BRENDAi4.2.1.49. 5092.

Miscellaneous databases

EvolutionaryTraceiP25080.

Family and domain databases

HAMAPiMF_00577. HutU.
InterProiIPR023637. Urocanase.
IPR023636. Urocanase_CS.
[Graphical view]
PfamiPF01175. Urocanase. 1 hit.
[Graphical view]
PIRSFiPIRSF001423. Urocanate_hydrat. 1 hit.
SUPFAMiSSF111326. SSF111326. 1 hit.
TIGRFAMsiTIGR01228. hutU. 1 hit.
PROSITEiPS01233. UROCANASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and sequencing the urocanase gene (hutU) from Pseudomonas putida."
    Fessemaier M., Frank R., Retey J., Schubert C.
    FEBS Lett. 286:55-57(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-23.
    Strain: NIC II.
  2. Allison S.L., Phillips A.T.
    Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 12633 / DSM 291 / JCM 13063 / NCIMB 9494 / NCTC 10936 / VKM B-2187 / Stanier 90.
  3. "Cloning, expression and mutational analysis of the urocanase gene (hutU) from Pseudomonas putida."
    Lenz M., Retey J.
    Eur. J. Biochem. 217:429-434(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS, MASS SPECTROMETRY.
    Strain: NIC II.

Entry informationi

Entry nameiHUTU_PSEPU
AccessioniPrimary (citable) accession number: P25080
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2007
Last modified: December 9, 2015
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.