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Protein

Malate dehydrogenase

Gene

mdh

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei34NADUniRule annotation1
Binding sitei81SubstrateUniRule annotation1
Binding sitei87SubstrateUniRule annotation1
Binding sitei94NADUniRule annotation1
Binding sitei119SubstrateUniRule annotation1
Binding sitei153SubstrateUniRule annotation1
Active sitei177Proton acceptorUniRule annotation1
Binding sitei227NADUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi7 – 13NADUniRule annotation7
Nucleotide bindingi117 – 119NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processTricarboxylic acid cycle
LigandNAD

Enzyme and pathway databases

BioCyciSENT99287:G1FZD-3393-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
Gene namesi
Name:mdhUniRule annotation
Ordered Locus Names:STM3359
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001133251 – 312Malate dehydrogenaseAdd BLAST312

Proteomic databases

PaxDbiP25077
PRIDEiP25077

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi99287.STM3359

Structurei

3D structure databases

ProteinModelPortaliP25077
SMRiP25077
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 1 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C80 Bacteria
COG0039 LUCA
HOGENOMiHOG000213792
KOiK00024
OMAiQCTPKVE
PhylomeDBiP25077

Family and domain databases

Gene3Di3.90.110.10, 1 hit
HAMAPiMF_01516 Malate_dehydrog_1, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR001252 Malate_DH_AS
IPR010097 Malate_DH_type1
IPR023958 Malate_DH_type1_bac
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR11540:SF22 PTHR11540:SF22, 1 hit
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01772 MDH_euk_gproteo, 1 hit
PROSITEiView protein in PROSITE
PS00068 MDH, 1 hit

Sequencei

Sequence statusi: Complete.

P25077-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVAVLGAAG GIGQALALLL KNQLPSGSEL SLYDIAPVTP GVAVDLSHIP
60 70 80 90 100
TAVKIKGFSG EDATPALEGA DVVLISAGVA RKPGMDRSDL FNVNAGIVKN
110 120 130 140 150
LVQQIAKTCP KACVGIITNP VNTTVAIAAE VLKKAGVYDK NKLFGVTTLD
160 170 180 190 200
IIRSNTFVAE LKGKLPTEVE VPVIGGHSGV TILPLLSQIP GVSFTEQEAA
210 220 230 240 250
ELTKRIQNAG TEVVEAKAGG GSATLSMGQA AARFGLSLVR ALQGEKGVVE
260 270 280 290 300
CAYVEGDGQY ARFFSQPLLL GKNGVEERKS IGTLSAFEQH SLDAMLDTLK
310
KDIQLGEDFI NK
Length:312
Mass (Da):32,476
Last modified:January 23, 2002 - v2
Checksum:i123BA51986AA838B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7G → A in AAA27158 (PubMed:8422999).Curated1
Sequence conflicti7G → A in CAA43363 (PubMed:8422999).Curated1
Sequence conflicti156T → P in AAA27158 (PubMed:8422999).Curated1
Sequence conflicti156T → P in CAA43363 (PubMed:8422999).Curated1
Sequence conflicti236L → V in AAA27158 (PubMed:8422999).Curated1
Sequence conflicti236L → V in CAA43363 (PubMed:8422999).Curated1
Sequence conflicti308 – 309DF → EI in AAA27158 (PubMed:8422999).Curated2
Sequence conflicti308 – 309DF → EI in CAA43363 (PubMed:8422999).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95049 Genomic DNA Translation: AAA27158.1
X61029 Genomic DNA Translation: CAA43363.1
AE006468 Genomic DNA Translation: AAL22228.1
PIRiS16143 DEEBM
RefSeqiNP_462269.1, NC_003197.2
WP_000861586.1, NC_003197.2

Genome annotation databases

EnsemblBacteriaiAAL22228; AAL22228; STM3359
GeneIDi1254882
KEGGistm:STM3359
PATRICifig|99287.12.peg.3560

Similar proteinsi

Entry informationi

Entry nameiMDH_SALTY
AccessioniPrimary (citable) accession number: P25077
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2002
Last modified: March 28, 2018
This is version 130 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health