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P25077

- MDH_SALTY

UniProt

P25077 - MDH_SALTY

Protein

Malate dehydrogenase

Gene

mdh

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 2 (23 Jan 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

    Catalytic activityi

    (S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei34 – 341NADUniRule annotation
    Binding sitei81 – 811SubstrateUniRule annotation
    Binding sitei87 – 871SubstrateUniRule annotation
    Binding sitei94 – 941NADUniRule annotation
    Binding sitei119 – 1191SubstrateUniRule annotation
    Binding sitei153 – 1531SubstrateUniRule annotation
    Active sitei177 – 1771Proton acceptorUniRule annotation
    Binding sitei227 – 2271NADUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi7 – 137NADUniRule annotation
    Nucleotide bindingi117 – 1193NADUniRule annotation

    GO - Molecular functioni

    1. L-malate dehydrogenase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cellular carbohydrate metabolic process Source: InterPro
    2. malate metabolic process Source: InterPro
    3. tricarboxylic acid cycle Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciSENT99287:GCTI-3380-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
    Gene namesi
    Name:mdhUniRule annotation
    Ordered Locus Names:STM3359
    OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
    Taxonomic identifieri99287 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
    ProteomesiUP000001014: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 312312Malate dehydrogenasePRO_0000113325Add
    BLAST

    Proteomic databases

    PRIDEiP25077.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    STRINGi99287.STM3359.

    Structurei

    3D structure databases

    ProteinModelPortaliP25077.
    SMRiP25077. Positions 1-312.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 1 family.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000213792.
    KOiK00024.
    OMAiKARGVYN.
    OrthoDBiEOG6091FG.
    PhylomeDBiP25077.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPiMF_01516. Malate_dehydrog_1.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR010097. Malate_DH_type1.
    IPR023958. Malate_DH_type1_bac.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11540. PTHR11540. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMiSSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
    PROSITEiPS00068. MDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P25077-1 [UniParc]FASTAAdd to Basket

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    MKVAVLGAAG GIGQALALLL KNQLPSGSEL SLYDIAPVTP GVAVDLSHIP    50
    TAVKIKGFSG EDATPALEGA DVVLISAGVA RKPGMDRSDL FNVNAGIVKN 100
    LVQQIAKTCP KACVGIITNP VNTTVAIAAE VLKKAGVYDK NKLFGVTTLD 150
    IIRSNTFVAE LKGKLPTEVE VPVIGGHSGV TILPLLSQIP GVSFTEQEAA 200
    ELTKRIQNAG TEVVEAKAGG GSATLSMGQA AARFGLSLVR ALQGEKGVVE 250
    CAYVEGDGQY ARFFSQPLLL GKNGVEERKS IGTLSAFEQH SLDAMLDTLK 300
    KDIQLGEDFI NK 312
    Length:312
    Mass (Da):32,476
    Last modified:January 23, 2002 - v2
    Checksum:i123BA51986AA838B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti7 – 71G → A in AAA27158. (PubMed:8422999)Curated
    Sequence conflicti7 – 71G → A in CAA43363. (PubMed:8422999)Curated
    Sequence conflicti156 – 1561T → P in AAA27158. (PubMed:8422999)Curated
    Sequence conflicti156 – 1561T → P in CAA43363. (PubMed:8422999)Curated
    Sequence conflicti236 – 2361L → V in AAA27158. (PubMed:8422999)Curated
    Sequence conflicti236 – 2361L → V in CAA43363. (PubMed:8422999)Curated
    Sequence conflicti308 – 3092DF → EI in AAA27158. (PubMed:8422999)Curated
    Sequence conflicti308 – 3092DF → EI in CAA43363. (PubMed:8422999)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M95049 Genomic DNA. Translation: AAA27158.1.
    X61029 Genomic DNA. Translation: CAA43363.1.
    AE006468 Genomic DNA. Translation: AAL22228.1.
    PIRiS16143. DEEBM.
    RefSeqiNP_462269.1. NC_003197.1.

    Genome annotation databases

    EnsemblBacteriaiAAL22228; AAL22228; STM3359.
    GeneIDi1254882.
    KEGGistm:STM3359.
    PATRICi32385513. VBISalEnt20916_3560.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M95049 Genomic DNA. Translation: AAA27158.1 .
    X61029 Genomic DNA. Translation: CAA43363.1 .
    AE006468 Genomic DNA. Translation: AAL22228.1 .
    PIRi S16143. DEEBM.
    RefSeqi NP_462269.1. NC_003197.1.

    3D structure databases

    ProteinModelPortali P25077.
    SMRi P25077. Positions 1-312.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 99287.STM3359.

    Proteomic databases

    PRIDEi P25077.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL22228 ; AAL22228 ; STM3359 .
    GeneIDi 1254882.
    KEGGi stm:STM3359.
    PATRICi 32385513. VBISalEnt20916_3560.

    Phylogenomic databases

    HOGENOMi HOG000213792.
    KOi K00024.
    OMAi KARGVYN.
    OrthoDBi EOG6091FG.
    PhylomeDBi P25077.

    Enzyme and pathway databases

    BioCyci SENT99287:GCTI-3380-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPi MF_01516. Malate_dehydrog_1.
    InterProi IPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR010097. Malate_DH_type1.
    IPR023958. Malate_DH_type1_bac.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11540. PTHR11540. 1 hit.
    Pfami PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMi SSF56327. SSF56327. 1 hit.
    TIGRFAMsi TIGR01772. MDH_euk_gproteo. 1 hit.
    PROSITEi PS00068. MDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of the Salmonella typhimurium gene encoding malate dehydrogenase."
      Lu C.-D., Abdelal A.T.
      Gene 123:143-144(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: LT2.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: LT2 / SGSC1412 / ATCC 700720.

    Entry informationi

    Entry nameiMDH_SALTY
    AccessioniPrimary (citable) accession number: P25077
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: January 23, 2002
    Last modified: October 1, 2014
    This is version 112 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3