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P25077

- MDH_SALTY

UniProt

P25077 - MDH_SALTY

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Protein

Malate dehydrogenase

Gene

mdh

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei34 – 341NADUniRule annotation
Binding sitei81 – 811SubstrateUniRule annotation
Binding sitei87 – 871SubstrateUniRule annotation
Binding sitei94 – 941NADUniRule annotation
Binding sitei119 – 1191SubstrateUniRule annotation
Binding sitei153 – 1531SubstrateUniRule annotation
Active sitei177 – 1771Proton acceptorUniRule annotation
Binding sitei227 – 2271NADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi7 – 137NADUniRule annotation
Nucleotide bindingi117 – 1193NADUniRule annotation

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. malate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciSENT99287:GCTI-3380-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
Gene namesi
Name:mdhUniRule annotation
Ordered Locus Names:STM3359
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000001014: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 312312Malate dehydrogenasePRO_0000113325Add
BLAST

Proteomic databases

PRIDEiP25077.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi99287.STM3359.

Structurei

3D structure databases

ProteinModelPortaliP25077.
SMRiP25077. Positions 1-312.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 1 family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000213792.
KOiK00024.
OMAiKARGVYN.
OrthoDBiEOG6091FG.
PhylomeDBiP25077.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01516. Malate_dehydrog_1.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR023958. Malate_DH_type1_bac.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25077-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKVAVLGAAG GIGQALALLL KNQLPSGSEL SLYDIAPVTP GVAVDLSHIP
60 70 80 90 100
TAVKIKGFSG EDATPALEGA DVVLISAGVA RKPGMDRSDL FNVNAGIVKN
110 120 130 140 150
LVQQIAKTCP KACVGIITNP VNTTVAIAAE VLKKAGVYDK NKLFGVTTLD
160 170 180 190 200
IIRSNTFVAE LKGKLPTEVE VPVIGGHSGV TILPLLSQIP GVSFTEQEAA
210 220 230 240 250
ELTKRIQNAG TEVVEAKAGG GSATLSMGQA AARFGLSLVR ALQGEKGVVE
260 270 280 290 300
CAYVEGDGQY ARFFSQPLLL GKNGVEERKS IGTLSAFEQH SLDAMLDTLK
310
KDIQLGEDFI NK
Length:312
Mass (Da):32,476
Last modified:January 23, 2002 - v2
Checksum:i123BA51986AA838B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti7 – 71G → A in AAA27158. (PubMed:8422999)Curated
Sequence conflicti7 – 71G → A in CAA43363. (PubMed:8422999)Curated
Sequence conflicti156 – 1561T → P in AAA27158. (PubMed:8422999)Curated
Sequence conflicti156 – 1561T → P in CAA43363. (PubMed:8422999)Curated
Sequence conflicti236 – 2361L → V in AAA27158. (PubMed:8422999)Curated
Sequence conflicti236 – 2361L → V in CAA43363. (PubMed:8422999)Curated
Sequence conflicti308 – 3092DF → EI in AAA27158. (PubMed:8422999)Curated
Sequence conflicti308 – 3092DF → EI in CAA43363. (PubMed:8422999)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95049 Genomic DNA. Translation: AAA27158.1.
X61029 Genomic DNA. Translation: CAA43363.1.
AE006468 Genomic DNA. Translation: AAL22228.1.
PIRiS16143. DEEBM.
RefSeqiNP_462269.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL22228; AAL22228; STM3359.
GeneIDi1254882.
KEGGistm:STM3359.
PATRICi32385513. VBISalEnt20916_3560.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95049 Genomic DNA. Translation: AAA27158.1 .
X61029 Genomic DNA. Translation: CAA43363.1 .
AE006468 Genomic DNA. Translation: AAL22228.1 .
PIRi S16143. DEEBM.
RefSeqi NP_462269.1. NC_003197.1.

3D structure databases

ProteinModelPortali P25077.
SMRi P25077. Positions 1-312.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 99287.STM3359.

Proteomic databases

PRIDEi P25077.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL22228 ; AAL22228 ; STM3359 .
GeneIDi 1254882.
KEGGi stm:STM3359.
PATRICi 32385513. VBISalEnt20916_3560.

Phylogenomic databases

HOGENOMi HOG000213792.
KOi K00024.
OMAi KARGVYN.
OrthoDBi EOG6091FG.
PhylomeDBi P25077.

Enzyme and pathway databases

BioCyci SENT99287:GCTI-3380-MONOMER.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPi MF_01516. Malate_dehydrog_1.
InterProi IPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR023958. Malate_DH_type1_bac.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR11540. PTHR11540. 1 hit.
Pfami PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMi SSF56327. SSF56327. 1 hit.
TIGRFAMsi TIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEi PS00068. MDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequence of the Salmonella typhimurium gene encoding malate dehydrogenase."
    Lu C.-D., Abdelal A.T.
    Gene 123:143-144(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: LT2.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.

Entry informationi

Entry nameiMDH_SALTY
AccessioniPrimary (citable) accession number: P25077
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2002
Last modified: October 1, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3