P25071 (CML12_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Calmodulin-like protein 12

Alternative name(s):
Touch-induced calmodulin-related protein 3

Gene names

Name:	CML12
Synonyms:	CAL4, TCH3
Ordered Locus Names:	At2g41100
ORF Names:	T3K9.13

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Potential calcium sensor that binds calcium in vitro.
Subunit structure	Interacts with PID. Ref.8
Induction	By auxin, rain-, wind-, and touch (thigmomorphogenesis) and during darkness conditions. Ref.8 Ref.9
Sequence similarities	Belongs to the calmodulin family. Contains 6 EF-hand domains.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	Calcium Metal-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to absence of light Inferred from expression pattern Ref.2. Source: TAIR thigmotropism Inferred from expression pattern Ref.3. Source: TAIR
Cellular_component	plasmodesma Inferred from direct assay PubMed 21533090. Source: TAIR vacuolar membrane Inferred from direct assay PubMed 17151019. Source: TAIR
Molecular_function	calcium ion binding Inferred from direct assay Ref.2. Source: TAIR
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PID	O64682	4	EBI-1238781,EBI-1393382

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P25071-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	Calmodulin-like protein 12		PRO_0000073657
Regions
	Domain	8 – 43	36	EF-hand 1
	Domain	44 – 79	36	EF-hand 2
	Domain	97 – 132	36	EF-hand 3
	Domain	133 – 168	36	EF-hand 4
	Domain	187 – 222	36	EF-hand 5
	Domain	223 – 258	36	EF-hand 6
	Calcium binding	21 – 32	12	1 Potential
	Calcium binding	57 – 68	12	2 Potential
	Calcium binding	110 – 121	12	3 Potential
	Calcium binding	146 – 157	12	4 Potential
	Calcium binding	200 – 211	12	5 Potential
	Calcium binding	236 – 247	12	6 Potential
Experimental info
	Sequence conflict	223 – 226	4	QTKA → KQKL in AAC37419. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 2, 2001. Version 3. Checksum: 94277BFBC2D2C5CE	FASTA	324	36,807
	10 20 30 40 50 60 MADKLTDDQI TEYRESFRLF DKNGDGSITK KELGTMMRSI GEKPTKADLQ DLMNEADLDG 70 80 90 100 110 120 DGTIDFPEFL CVMAKNQGHD QAPRHTKKTM ADKLTDDQIT EYRESFRLFD KNGDGSITKK 130 140 150 160 170 180 ELRTVMFSLG KNRTKADLQD MMNEVDLDGD GTIDFPEFLY LMAKNQGHDQ APRHTKKTMV 190 200 210 220 230 240 DYQLTDDQIL EFREAFRVFD KNGDGYITVN ELRTTMRSLG ETQTKAELQD MINEADADGD 250 260 270 280 290 300 GTISFSEFVC VMTGKMIDTQ SKKETYRVVN QGQGQVQRHT RNDRAGGTNW ERDIAVGVAS 310 320 NIIASPISDF MKDRFKDLFE ALLS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rain-, wind-, and touch-induced expression of calmodulin and calmodulin-related genes in Arabidopsis." Braam J., Davis R.W. Cell 60:357-364(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Arabidopsis TCH3 encodes a novel Ca2+ binding protein and shows environmentally induced and tissue-specific regulation." Sistrunk M.L., Antosiewicz D.M., Purugganan M.M., Braam J. Plant Cell 6:1553-1565(1994) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION.
[3]	"Touch-inducible genes for calmodulin and a calmodulin-related protein are located in tandem on a chromosome of Arabidopsis thaliana." Ito T., Hirano M., Akama K., Shimura Y., Okada K. Plant Cell Physiol. 36:1369-1373(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Landsberg erecta.
[4]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[5]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[6]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[7]	"Calmodulins and related potential calcium sensors of Arabidopsis." McCormack E., Braam J. New Phytol. 159:585-598(2003) Cited for: GENE FAMILY, NOMENCLATURE.
[8]	"PINOID-mediated signaling involves calcium-binding proteins." Benjamins R., Ampudia C.S., Hooykaas P.J., Offringa R. Plant Physiol. 132:1623-1630(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PID, INDUCTION BY AUXIN.
[9]	"Genome-wide identification of touch- and darkness-regulated Arabidopsis genes: a focus on calmodulin-like and XTH genes." Lee D., Polisensky D.H., Braam J. New Phytol. 165:429-444(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L34546 Genomic DNA. Translation: AAC37419.1. D45848 Genomic DNA. Translation: BAA08282.1. AC004261 Genomic DNA. Translation: AAD12001.1. CP002685 Genomic DNA. Translation: AEC09928.1. CP002685 Genomic DNA. Translation: AEC09931.1. AF424577 mRNA. Translation: AAL11571.1. AY120719 mRNA. Translation: AAM53277.1. BT000036 mRNA. Translation: AAN15355.1.
IPI	IPI00518580.
PIR	B34669. T02109.
RefSeq	NP_001189723.1. NM_001202794.1. NP_181643.1. NM_129675.4.
UniGene	At.12547. At.269.
3D structure databases
ProteinModelPortal	P25071.
SMR	P25071. Positions 2-278.
ModBase	Search...
Protein-protein interaction databases
IntAct	P25071. 89 interactions.
STRING	3702.AT2G41100.1-P.
Proteomic databases
PaxDb	P25071.
PRIDE	P25071.
Protocols and materials databases
DNASU	818709.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G41100.1; AT2G41100.1; AT2G41100. AT2G41100.4; AT2G41100.4; AT2G41100.
GeneID	818709.
KEGG	ath:AT2G41100.
Organism-specific databases
TAIR	At2g41100.
Phylogenomic databases
eggNOG	COG5126.
HOGENOM	HOG000233018.
InParanoid	P25071.
KO	K13448.
OMA	MINEADA.
PhylomeDB	P25071.
ProtClustDB	CLSN2683659.
Gene expression databases
ArrayExpress	P25071.
Genevestigator	P25071.
Family and domain databases
Gene3D	1.10.238.10. 2 hits.
InterPro	IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. [Graphical view]
Pfam	PF13499. EF_hand_5. 1 hit. PF00036. efhand. 4 hits. [Graphical view]
SMART	SM00054. EFh. 6 hits. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 6 hits. PS50222. EF_HAND_2. 6 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CML12_ARATH

Accession

Primary (citable) accession number: P25071
Secondary accession number(s): Q38972, Q39064, Q8H1A0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	November 2, 2001
Last modified:	May 1, 2013
This is version 113 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program