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P25071

- CML12_ARATH

UniProt

P25071 - CML12_ARATH

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Protein
Calmodulin-like protein 12
Gene
CML12, CAL4, TCH3, At2g41100, T3K9.13
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Potential calcium sensor that binds calcium in vitro.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi21 – 32121 Reviewed prediction
Add
BLAST
Calcium bindingi57 – 68122 Reviewed prediction
Add
BLAST
Calcium bindingi110 – 121123 Reviewed prediction
Add
BLAST
Calcium bindingi146 – 157124 Reviewed prediction
Add
BLAST
Calcium bindingi200 – 211125 Reviewed prediction
Add
BLAST
Calcium bindingi236 – 247126 Reviewed prediction
Add
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: TAIR
  2. protein binding Source: IntAct

GO - Biological processi

  1. response to absence of light Source: TAIR
  2. response to mechanical stimulus Source: TAIR
  3. thigmotropism Source: TAIR
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calmodulin-like protein 12
Alternative name(s):
Touch-induced calmodulin-related protein 3
Gene namesi
Name:CML12
Synonyms:CAL4, TCH3
Ordered Locus Names:At2g41100
ORF Names:T3K9.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G41100.

Subcellular locationi

GO - Cellular componenti

  1. plasmodesma Source: TAIR
  2. vacuolar membrane Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 324324Calmodulin-like protein 12
PRO_0000073657Add
BLAST

Proteomic databases

PaxDbiP25071.
PRIDEiP25071.

Expressioni

Inductioni

By auxin, rain-, wind-, and touch (thigmomorphogenesis) and during darkness conditions.2 Publications

Gene expression databases

ArrayExpressiP25071.
GenevestigatoriP25071.

Interactioni

Subunit structurei

Interacts with PID.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PIDO646824EBI-1238781,EBI-1393382

Protein-protein interaction databases

BioGridi4046. 3 interactions.
IntActiP25071. 89 interactions.
STRINGi3702.AT2G41100.1-P.

Structurei

3D structure databases

ProteinModelPortaliP25071.
SMRiP25071. Positions 2-312.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 4336EF-hand 1
Add
BLAST
Domaini44 – 7936EF-hand 2
Add
BLAST
Domaini97 – 13236EF-hand 3
Add
BLAST
Domaini133 – 16836EF-hand 4
Add
BLAST
Domaini187 – 22236EF-hand 5
Add
BLAST
Domaini223 – 25836EF-hand 6
Add
BLAST

Sequence similaritiesi

Belongs to the calmodulin family.
Contains 6 EF-hand domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5126.
HOGENOMiHOG000233018.
InParanoidiP25071.
KOiK13448.
OMAiMINEADA.
PhylomeDBiP25071.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF00036. EF-hand_1. 4 hits.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 6 hits.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 6 hits.
PS50222. EF_HAND_2. 6 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P25071-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MADKLTDDQI TEYRESFRLF DKNGDGSITK KELGTMMRSI GEKPTKADLQ    50
DLMNEADLDG DGTIDFPEFL CVMAKNQGHD QAPRHTKKTM ADKLTDDQIT 100
EYRESFRLFD KNGDGSITKK ELRTVMFSLG KNRTKADLQD MMNEVDLDGD 150
GTIDFPEFLY LMAKNQGHDQ APRHTKKTMV DYQLTDDQIL EFREAFRVFD 200
KNGDGYITVN ELRTTMRSLG ETQTKAELQD MINEADADGD GTISFSEFVC 250
VMTGKMIDTQ SKKETYRVVN QGQGQVQRHT RNDRAGGTNW ERDIAVGVAS 300
NIIASPISDF MKDRFKDLFE ALLS 324
Length:324
Mass (Da):36,807
Last modified:November 2, 2001 - v3
Checksum:i94277BFBC2D2C5CE
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti223 – 2264QTKA → KQKL in AAC37419. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L34546 Genomic DNA. Translation: AAC37419.1.
D45848 Genomic DNA. Translation: BAA08282.1.
AC004261 Genomic DNA. Translation: AAD12001.1.
CP002685 Genomic DNA. Translation: AEC09928.1.
CP002685 Genomic DNA. Translation: AEC09931.1.
AF424577 mRNA. Translation: AAL11571.1.
AY120719 mRNA. Translation: AAM53277.1.
BT000036 mRNA. Translation: AAN15355.1.
PIRiB34669.
T02109.
RefSeqiNP_001189723.1. NM_001202794.1. [P25071-1]
NP_181643.1. NM_129675.4. [P25071-1]
UniGeneiAt.12547.
At.269.

Genome annotation databases

EnsemblPlantsiAT2G41100.1; AT2G41100.1; AT2G41100. [P25071-1]
AT2G41100.4; AT2G41100.4; AT2G41100. [P25071-1]
GeneIDi818709.
KEGGiath:AT2G41100.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L34546 Genomic DNA. Translation: AAC37419.1 .
D45848 Genomic DNA. Translation: BAA08282.1 .
AC004261 Genomic DNA. Translation: AAD12001.1 .
CP002685 Genomic DNA. Translation: AEC09928.1 .
CP002685 Genomic DNA. Translation: AEC09931.1 .
AF424577 mRNA. Translation: AAL11571.1 .
AY120719 mRNA. Translation: AAM53277.1 .
BT000036 mRNA. Translation: AAN15355.1 .
PIRi B34669.
T02109.
RefSeqi NP_001189723.1. NM_001202794.1. [P25071-1 ]
NP_181643.1. NM_129675.4. [P25071-1 ]
UniGenei At.12547.
At.269.

3D structure databases

ProteinModelPortali P25071.
SMRi P25071. Positions 2-312.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 4046. 3 interactions.
IntActi P25071. 89 interactions.
STRINGi 3702.AT2G41100.1-P.

Proteomic databases

PaxDbi P25071.
PRIDEi P25071.

Protocols and materials databases

DNASUi 818709.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G41100.1 ; AT2G41100.1 ; AT2G41100 . [P25071-1 ]
AT2G41100.4 ; AT2G41100.4 ; AT2G41100 . [P25071-1 ]
GeneIDi 818709.
KEGGi ath:AT2G41100.

Organism-specific databases

TAIRi AT2G41100.

Phylogenomic databases

eggNOGi COG5126.
HOGENOMi HOG000233018.
InParanoidi P25071.
KOi K13448.
OMAi MINEADA.
PhylomeDBi P25071.

Gene expression databases

ArrayExpressi P25071.
Genevestigatori P25071.

Family and domain databases

Gene3Di 1.10.238.10. 2 hits.
InterProi IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view ]
Pfami PF00036. EF-hand_1. 4 hits.
PF13499. EF-hand_7. 1 hit.
[Graphical view ]
SMARTi SM00054. EFh. 6 hits.
[Graphical view ]
PROSITEi PS00018. EF_HAND_1. 6 hits.
PS50222. EF_HAND_2. 6 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Rain-, wind-, and touch-induced expression of calmodulin and calmodulin-related genes in Arabidopsis."
    Braam J., Davis R.W.
    Cell 60:357-364(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
  2. "Arabidopsis TCH3 encodes a novel Ca2+ binding protein and shows environmentally induced and tissue-specific regulation."
    Sistrunk M.L., Antosiewicz D.M., Purugganan M.M., Braam J.
    Plant Cell 6:1553-1565(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION.
  3. "Touch-inducible genes for calmodulin and a calmodulin-related protein are located in tandem on a chromosome of Arabidopsis thaliana."
    Ito T., Hirano M., Akama K., Shimura Y., Okada K.
    Plant Cell Physiol. 36:1369-1373(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Landsberg erecta.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  5. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  7. "Calmodulins and related potential calcium sensors of Arabidopsis."
    McCormack E., Braam J.
    New Phytol. 159:585-598(2003)
    Cited for: GENE FAMILY, NOMENCLATURE.
  8. "PINOID-mediated signaling involves calcium-binding proteins."
    Benjamins R., Ampudia C.S., Hooykaas P.J., Offringa R.
    Plant Physiol. 132:1623-1630(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PID, INDUCTION BY AUXIN.
  9. "Genome-wide identification of touch- and darkness-regulated Arabidopsis genes: a focus on calmodulin-like and XTH genes."
    Lee D., Polisensky D.H., Braam J.
    New Phytol. 165:429-444(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiCML12_ARATH
AccessioniPrimary (citable) accession number: P25071
Secondary accession number(s): Q38972, Q39064, Q8H1A0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: November 2, 2001
Last modified: June 11, 2014
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi