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P25071 (CML12_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Calmodulin-like protein 12
Alternative name(s):
Touch-induced calmodulin-related protein 3
Gene names
Name:CML12
Synonyms:CAL4, TCH3
Ordered Locus Names:At2g41100
ORF Names:T3K9.13
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length324 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Potential calcium sensor that binds calcium in vitro.

Subunit structure

Interacts with PID. Ref.8

Induction

By auxin, rain-, wind-, and touch (thigmomorphogenesis) and during darkness conditions. Ref.8 Ref.9

Sequence similarities

Belongs to the calmodulin family.

Contains 6 EF-hand domains.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PIDO646824EBI-1238781,EBI-1393382

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P25071-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 324324Calmodulin-like protein 12
PRO_0000073657

Regions

Domain8 – 4336EF-hand 1
Domain44 – 7936EF-hand 2
Domain97 – 13236EF-hand 3
Domain133 – 16836EF-hand 4
Domain187 – 22236EF-hand 5
Domain223 – 25836EF-hand 6
Calcium binding21 – 32121 Potential
Calcium binding57 – 68122 Potential
Calcium binding110 – 121123 Potential
Calcium binding146 – 157124 Potential
Calcium binding200 – 211125 Potential
Calcium binding236 – 247126 Potential

Experimental info

Sequence conflict223 – 2264QTKA → KQKL in AAC37419. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 2, 2001. Version 3.
Checksum: 94277BFBC2D2C5CE

FASTA32436,807
        10         20         30         40         50         60 
MADKLTDDQI TEYRESFRLF DKNGDGSITK KELGTMMRSI GEKPTKADLQ DLMNEADLDG 

        70         80         90        100        110        120 
DGTIDFPEFL CVMAKNQGHD QAPRHTKKTM ADKLTDDQIT EYRESFRLFD KNGDGSITKK 

       130        140        150        160        170        180 
ELRTVMFSLG KNRTKADLQD MMNEVDLDGD GTIDFPEFLY LMAKNQGHDQ APRHTKKTMV 

       190        200        210        220        230        240 
DYQLTDDQIL EFREAFRVFD KNGDGYITVN ELRTTMRSLG ETQTKAELQD MINEADADGD 

       250        260        270        280        290        300 
GTISFSEFVC VMTGKMIDTQ SKKETYRVVN QGQGQVQRHT RNDRAGGTNW ERDIAVGVAS 

       310        320 
NIIASPISDF MKDRFKDLFE ALLS 

« Hide

References

« Hide 'large scale' references
[1]"Rain-, wind-, and touch-induced expression of calmodulin and calmodulin-related genes in Arabidopsis."
Braam J., Davis R.W.
Cell 60:357-364(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Columbia.
[2]"Arabidopsis TCH3 encodes a novel Ca2+ binding protein and shows environmentally induced and tissue-specific regulation."
Sistrunk M.L., Antosiewicz D.M., Purugganan M.M., Braam J.
Plant Cell 6:1553-1565(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION.
[3]"Touch-inducible genes for calmodulin and a calmodulin-related protein are located in tandem on a chromosome of Arabidopsis thaliana."
Ito T., Hirano M., Akama K., Shimura Y., Okada K.
Plant Cell Physiol. 36:1369-1373(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Landsberg erecta.
[4]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[5]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[6]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[7]"Calmodulins and related potential calcium sensors of Arabidopsis."
McCormack E., Braam J.
New Phytol. 159:585-598(2003)
Cited for: GENE FAMILY, NOMENCLATURE.
[8]"PINOID-mediated signaling involves calcium-binding proteins."
Benjamins R., Ampudia C.S., Hooykaas P.J., Offringa R.
Plant Physiol. 132:1623-1630(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PID, INDUCTION BY AUXIN.
[9]"Genome-wide identification of touch- and darkness-regulated Arabidopsis genes: a focus on calmodulin-like and XTH genes."
Lee D., Polisensky D.H., Braam J.
New Phytol. 165:429-444(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L34546 Genomic DNA. Translation: AAC37419.1.
D45848 Genomic DNA. Translation: BAA08282.1.
AC004261 Genomic DNA. Translation: AAD12001.1.
CP002685 Genomic DNA. Translation: AEC09928.1.
CP002685 Genomic DNA. Translation: AEC09931.1.
AF424577 mRNA. Translation: AAL11571.1.
AY120719 mRNA. Translation: AAM53277.1.
BT000036 mRNA. Translation: AAN15355.1.
PIRB34669.
T02109.
RefSeqNP_001189723.1. NM_001202794.1. [P25071-1]
NP_181643.1. NM_129675.4. [P25071-1]
UniGeneAt.12547.
At.269.

3D structure databases

ProteinModelPortalP25071.
SMRP25071. Positions 2-312.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid4046. 3 interactions.
IntActP25071. 89 interactions.
STRING3702.AT2G41100.1-P.

Proteomic databases

PaxDbP25071.
PRIDEP25071.

Protocols and materials databases

DNASU818709.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G41100.1; AT2G41100.1; AT2G41100. [P25071-1]
AT2G41100.4; AT2G41100.4; AT2G41100. [P25071-1]
GeneID818709.
KEGGath:AT2G41100.

Organism-specific databases

TAIRAT2G41100.

Phylogenomic databases

eggNOGCOG5126.
HOGENOMHOG000233018.
InParanoidP25071.
KOK13448.
OMAMINEADA.
PhylomeDBP25071.

Gene expression databases

ArrayExpressP25071.
GenevestigatorP25071.

Family and domain databases

Gene3D1.10.238.10. 2 hits.
InterProIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamPF00036. EF-hand_1. 4 hits.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTSM00054. EFh. 6 hits.
[Graphical view]
PROSITEPS00018. EF_HAND_1. 6 hits.
PS50222. EF_HAND_2. 6 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCML12_ARATH
AccessionPrimary (citable) accession number: P25071
Secondary accession number(s): Q38972, Q39064, Q8H1A0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: November 2, 2001
Last modified: June 11, 2014
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names