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P25071

- CML12_ARATH

UniProt

P25071 - CML12_ARATH

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Protein

Calmodulin-like protein 12

Gene

CML12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Potential calcium sensor that binds calcium in vitro.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi21 – 32121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi57 – 68122PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi110 – 121123PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi146 – 157124PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi200 – 211125PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi236 – 247126PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: TAIR

GO - Biological processi

  1. response to absence of light Source: TAIR
  2. response to mechanical stimulus Source: TAIR
  3. thigmotropism Source: TAIR
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calmodulin-like protein 12
Alternative name(s):
Touch-induced calmodulin-related protein 3
Gene namesi
Name:CML12
Synonyms:CAL4, TCH3
Ordered Locus Names:At2g41100
ORF Names:T3K9.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G41100.

Subcellular locationi

GO - Cellular componenti

  1. plasmodesma Source: TAIR
  2. vacuolar membrane Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 324324Calmodulin-like protein 12PRO_0000073657Add
BLAST

Proteomic databases

PaxDbiP25071.
PRIDEiP25071.

Expressioni

Inductioni

By auxin, rain-, wind-, and touch (thigmomorphogenesis) and during darkness conditions.2 Publications

Gene expression databases

ExpressionAtlasiP25071. baseline and differential.
GenevestigatoriP25071.

Interactioni

Subunit structurei

Interacts with PID.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PIDO646824EBI-1238781,EBI-1393382

Protein-protein interaction databases

BioGridi4046. 3 interactions.
IntActiP25071. 89 interactions.
STRINGi3702.AT2G41100.1-P.

Structurei

3D structure databases

ProteinModelPortaliP25071.
SMRiP25071. Positions 2-312.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 4336EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini44 – 7936EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini97 – 13236EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini133 – 16836EF-hand 4PROSITE-ProRule annotationAdd
BLAST
Domaini187 – 22236EF-hand 5PROSITE-ProRule annotationAdd
BLAST
Domaini223 – 25836EF-hand 6PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the calmodulin family.Curated
Contains 6 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5126.
HOGENOMiHOG000233018.
InParanoidiP25071.
KOiK13448.
OMAiMINEADA.
PhylomeDBiP25071.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF00036. EF-hand_1. 4 hits.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 6 hits.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 6 hits.
PS50222. EF_HAND_2. 6 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P25071-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADKLTDDQI TEYRESFRLF DKNGDGSITK KELGTMMRSI GEKPTKADLQ
60 70 80 90 100
DLMNEADLDG DGTIDFPEFL CVMAKNQGHD QAPRHTKKTM ADKLTDDQIT
110 120 130 140 150
EYRESFRLFD KNGDGSITKK ELRTVMFSLG KNRTKADLQD MMNEVDLDGD
160 170 180 190 200
GTIDFPEFLY LMAKNQGHDQ APRHTKKTMV DYQLTDDQIL EFREAFRVFD
210 220 230 240 250
KNGDGYITVN ELRTTMRSLG ETQTKAELQD MINEADADGD GTISFSEFVC
260 270 280 290 300
VMTGKMIDTQ SKKETYRVVN QGQGQVQRHT RNDRAGGTNW ERDIAVGVAS
310 320
NIIASPISDF MKDRFKDLFE ALLS
Length:324
Mass (Da):36,807
Last modified:November 2, 2001 - v3
Checksum:i94277BFBC2D2C5CE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti223 – 2264QTKA → KQKL in AAC37419. (PubMed:2302732)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L34546 Genomic DNA. Translation: AAC37419.1.
D45848 Genomic DNA. Translation: BAA08282.1.
AC004261 Genomic DNA. Translation: AAD12001.1.
CP002685 Genomic DNA. Translation: AEC09928.1.
CP002685 Genomic DNA. Translation: AEC09931.1.
AF424577 mRNA. Translation: AAL11571.1.
AY120719 mRNA. Translation: AAM53277.1.
BT000036 mRNA. Translation: AAN15355.1.
PIRiB34669.
T02109.
RefSeqiNP_001189723.1. NM_001202794.1. [P25071-1]
NP_181643.1. NM_129675.4. [P25071-1]
UniGeneiAt.12547.
At.269.

Genome annotation databases

EnsemblPlantsiAT2G41100.1; AT2G41100.1; AT2G41100. [P25071-1]
AT2G41100.4; AT2G41100.4; AT2G41100. [P25071-1]
GeneIDi818709.
KEGGiath:AT2G41100.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L34546 Genomic DNA. Translation: AAC37419.1 .
D45848 Genomic DNA. Translation: BAA08282.1 .
AC004261 Genomic DNA. Translation: AAD12001.1 .
CP002685 Genomic DNA. Translation: AEC09928.1 .
CP002685 Genomic DNA. Translation: AEC09931.1 .
AF424577 mRNA. Translation: AAL11571.1 .
AY120719 mRNA. Translation: AAM53277.1 .
BT000036 mRNA. Translation: AAN15355.1 .
PIRi B34669.
T02109.
RefSeqi NP_001189723.1. NM_001202794.1. [P25071-1 ]
NP_181643.1. NM_129675.4. [P25071-1 ]
UniGenei At.12547.
At.269.

3D structure databases

ProteinModelPortali P25071.
SMRi P25071. Positions 2-312.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 4046. 3 interactions.
IntActi P25071. 89 interactions.
STRINGi 3702.AT2G41100.1-P.

Proteomic databases

PaxDbi P25071.
PRIDEi P25071.

Protocols and materials databases

DNASUi 818709.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G41100.1 ; AT2G41100.1 ; AT2G41100 . [P25071-1 ]
AT2G41100.4 ; AT2G41100.4 ; AT2G41100 . [P25071-1 ]
GeneIDi 818709.
KEGGi ath:AT2G41100.

Organism-specific databases

TAIRi AT2G41100.

Phylogenomic databases

eggNOGi COG5126.
HOGENOMi HOG000233018.
InParanoidi P25071.
KOi K13448.
OMAi MINEADA.
PhylomeDBi P25071.

Gene expression databases

ExpressionAtlasi P25071. baseline and differential.
Genevestigatori P25071.

Family and domain databases

Gene3Di 1.10.238.10. 2 hits.
InterProi IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view ]
Pfami PF00036. EF-hand_1. 4 hits.
PF13499. EF-hand_7. 1 hit.
[Graphical view ]
SMARTi SM00054. EFh. 6 hits.
[Graphical view ]
PROSITEi PS00018. EF_HAND_1. 6 hits.
PS50222. EF_HAND_2. 6 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Rain-, wind-, and touch-induced expression of calmodulin and calmodulin-related genes in Arabidopsis."
    Braam J., Davis R.W.
    Cell 60:357-364(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
  2. "Arabidopsis TCH3 encodes a novel Ca2+ binding protein and shows environmentally induced and tissue-specific regulation."
    Sistrunk M.L., Antosiewicz D.M., Purugganan M.M., Braam J.
    Plant Cell 6:1553-1565(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION.
  3. "Touch-inducible genes for calmodulin and a calmodulin-related protein are located in tandem on a chromosome of Arabidopsis thaliana."
    Ito T., Hirano M., Akama K., Shimura Y., Okada K.
    Plant Cell Physiol. 36:1369-1373(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Landsberg erecta.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  5. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  7. "Calmodulins and related potential calcium sensors of Arabidopsis."
    McCormack E., Braam J.
    New Phytol. 159:585-598(2003)
    Cited for: GENE FAMILY, NOMENCLATURE.
  8. "PINOID-mediated signaling involves calcium-binding proteins."
    Benjamins R., Ampudia C.S., Hooykaas P.J., Offringa R.
    Plant Physiol. 132:1623-1630(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PID, INDUCTION BY AUXIN.
  9. "Genome-wide identification of touch- and darkness-regulated Arabidopsis genes: a focus on calmodulin-like and XTH genes."
    Lee D., Polisensky D.H., Braam J.
    New Phytol. 165:429-444(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiCML12_ARATH
AccessioniPrimary (citable) accession number: P25071
Secondary accession number(s): Q38972, Q39064, Q8H1A0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: November 2, 2001
Last modified: October 29, 2014
This is version 124 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3