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Protein

Calmodulin-like protein 12

Gene

CML12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Potential calcium sensor that binds calcium in vitro.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi21 – 321PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi57 – 682PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi110 – 1213PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi146 – 1574PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi200 – 2115PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi236 – 2476PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

  • calcium ion binding Source: TAIR

GO - Biological processi

  • response to absence of light Source: TAIR
  • response to mechanical stimulus Source: TAIR
  • thigmotropism Source: TAIR

Keywordsi

LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-ATH-114608. Platelet degranulation.
R-ATH-2514859. Inactivation, recovery and regulation of the phototransduction cascade.
R-ATH-418359. Reduction of cytosolic Ca++ levels.
R-ATH-425561. Sodium/Calcium exchangers.
R-ATH-5578775. Ion homeostasis.
R-ATH-8876725. Protein methylation.

Names & Taxonomyi

Protein namesi
Recommended name:
Calmodulin-like protein 12
Alternative name(s):
Touch-induced calmodulin-related protein 3
Gene namesi
Name:CML12
Synonyms:CAL4, TCH3
Ordered Locus Names:At2g41100
ORF Names:T3K9.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G41100.
TAIRilocus:2063230. AT2G41100.

Subcellular locationi

GO - Cellular componenti

  • plasmodesma Source: TAIR
  • vacuolar membrane Source: TAIR

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000736571 – 324Calmodulin-like protein 12Add BLAST324

Proteomic databases

PaxDbiP25071.
PRIDEiP25071.

PTM databases

iPTMnetiP25071.

Expressioni

Inductioni

By auxin, rain-, wind-, and touch (thigmomorphogenesis) and during darkness conditions.2 Publications

Gene expression databases

ExpressionAtlasiP25071. baseline and differential.
GenevisibleiP25071. AT.

Interactioni

Subunit structurei

Interacts with PID.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PIDO646824EBI-1238781,EBI-1393382

Protein-protein interaction databases

BioGridi4046. 4 interactors.
IntActiP25071. 89 interactors.
STRINGi3702.AT2G41100.1.

Structurei

3D structure databases

ProteinModelPortaliP25071.
SMRiP25071.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 43EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini44 – 79EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini97 – 132EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini133 – 168EF-hand 4PROSITE-ProRule annotationAdd BLAST36
Domaini187 – 222EF-hand 5PROSITE-ProRule annotationAdd BLAST36
Domaini223 – 258EF-hand 6PROSITE-ProRule annotationAdd BLAST36

Sequence similaritiesi

Belongs to the calmodulin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0027. Eukaryota.
COG5126. LUCA.
HOGENOMiHOG000233018.
InParanoidiP25071.
KOiK13448.
OMAiQITEYRE.
OrthoDBiEOG09360QIJ.
PhylomeDBiP25071.

Family and domain databases

CDDicd00051. EFh. 3 hits.
InterProiView protein in InterPro
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
PfamiView protein in Pfam
PF13499. EF-hand_7. 3 hits.
SMARTiView protein in SMART
SM00054. EFh. 6 hits.
SUPFAMiSSF47473. SSF47473. 2 hits.
PROSITEiView protein in PROSITE
PS00018. EF_HAND_1. 6 hits.
PS50222. EF_HAND_2. 6 hits.

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P25071-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADKLTDDQI TEYRESFRLF DKNGDGSITK KELGTMMRSI GEKPTKADLQ
60 70 80 90 100
DLMNEADLDG DGTIDFPEFL CVMAKNQGHD QAPRHTKKTM ADKLTDDQIT
110 120 130 140 150
EYRESFRLFD KNGDGSITKK ELRTVMFSLG KNRTKADLQD MMNEVDLDGD
160 170 180 190 200
GTIDFPEFLY LMAKNQGHDQ APRHTKKTMV DYQLTDDQIL EFREAFRVFD
210 220 230 240 250
KNGDGYITVN ELRTTMRSLG ETQTKAELQD MINEADADGD GTISFSEFVC
260 270 280 290 300
VMTGKMIDTQ SKKETYRVVN QGQGQVQRHT RNDRAGGTNW ERDIAVGVAS
310 320
NIIASPISDF MKDRFKDLFE ALLS
Length:324
Mass (Da):36,807
Last modified:November 2, 2001 - v3
Checksum:i94277BFBC2D2C5CE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti223 – 226QTKA → KQKL in AAC37419 (PubMed:2302732).Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34546 Genomic DNA. Translation: AAC37419.1.
D45848 Genomic DNA. Translation: BAA08282.1.
AC004261 Genomic DNA. Translation: AAD12001.1.
CP002685 Genomic DNA. Translation: AEC09928.1.
CP002685 Genomic DNA. Translation: AEC09931.1.
AF424577 mRNA. Translation: AAL11571.1.
AY120719 mRNA. Translation: AAM53277.1.
BT000036 mRNA. Translation: AAN15355.1.
PIRiB34669.
T02109.
RefSeqiNP_001189723.1. NM_001202794.1. [P25071-1]
NP_181643.1. NM_129675.5. [P25071-1]
UniGeneiAt.12547.
At.269.

Genome annotation databases

EnsemblPlantsiAT2G41100.1; AT2G41100.1; AT2G41100. [P25071-1]
AT2G41100.4; AT2G41100.4; AT2G41100. [P25071-1]
GeneIDi818709.
GrameneiAT2G41100.1; AT2G41100.1; AT2G41100.
AT2G41100.4; AT2G41100.4; AT2G41100.
KEGGiath:AT2G41100.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCML12_ARATH
AccessioniPrimary (citable) accession number: P25071
Secondary accession number(s): Q38972, Q39064, Q8H1A0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: November 2, 2001
Last modified: August 30, 2017
This is version 146 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families