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P25071

- CML12_ARATH

UniProt

P25071 - CML12_ARATH

Protein

Calmodulin-like protein 12

Gene

CML12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 3 (02 Nov 2001)
      Previous versions | rss
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    Functioni

    Potential calcium sensor that binds calcium in vitro.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi21 – 32121PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi57 – 68122PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi110 – 121123PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi146 – 157124PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi200 – 211125PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi236 – 247126PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: TAIR
    2. protein binding Source: IntAct

    GO - Biological processi

    1. response to absence of light Source: TAIR
    2. response to mechanical stimulus Source: TAIR
    3. thigmotropism Source: TAIR

    Keywords - Ligandi

    Calcium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calmodulin-like protein 12
    Alternative name(s):
    Touch-induced calmodulin-related protein 3
    Gene namesi
    Name:CML12
    Synonyms:CAL4, TCH3
    Ordered Locus Names:At2g41100
    ORF Names:T3K9.13
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G41100.

    Subcellular locationi

    GO - Cellular componenti

    1. plasmodesma Source: TAIR
    2. vacuolar membrane Source: TAIR

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 324324Calmodulin-like protein 12PRO_0000073657Add
    BLAST

    Proteomic databases

    PaxDbiP25071.
    PRIDEiP25071.

    Expressioni

    Inductioni

    By auxin, rain-, wind-, and touch (thigmomorphogenesis) and during darkness conditions.2 Publications

    Gene expression databases

    ArrayExpressiP25071.
    GenevestigatoriP25071.

    Interactioni

    Subunit structurei

    Interacts with PID.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PIDO646824EBI-1238781,EBI-1393382

    Protein-protein interaction databases

    BioGridi4046. 3 interactions.
    IntActiP25071. 89 interactions.
    STRINGi3702.AT2G41100.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliP25071.
    SMRiP25071. Positions 2-312.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini8 – 4336EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini44 – 7936EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini97 – 13236EF-hand 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini133 – 16836EF-hand 4PROSITE-ProRule annotationAdd
    BLAST
    Domaini187 – 22236EF-hand 5PROSITE-ProRule annotationAdd
    BLAST
    Domaini223 – 25836EF-hand 6PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the calmodulin family.Curated
    Contains 6 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG5126.
    HOGENOMiHOG000233018.
    InParanoidiP25071.
    KOiK13448.
    OMAiMINEADA.
    PhylomeDBiP25071.

    Family and domain databases

    Gene3Di1.10.238.10. 2 hits.
    InterProiIPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    [Graphical view]
    PfamiPF00036. EF-hand_1. 4 hits.
    PF13499. EF-hand_7. 1 hit.
    [Graphical view]
    SMARTiSM00054. EFh. 6 hits.
    [Graphical view]
    PROSITEiPS00018. EF_HAND_1. 6 hits.
    PS50222. EF_HAND_2. 6 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: P25071-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MADKLTDDQI TEYRESFRLF DKNGDGSITK KELGTMMRSI GEKPTKADLQ    50
    DLMNEADLDG DGTIDFPEFL CVMAKNQGHD QAPRHTKKTM ADKLTDDQIT 100
    EYRESFRLFD KNGDGSITKK ELRTVMFSLG KNRTKADLQD MMNEVDLDGD 150
    GTIDFPEFLY LMAKNQGHDQ APRHTKKTMV DYQLTDDQIL EFREAFRVFD 200
    KNGDGYITVN ELRTTMRSLG ETQTKAELQD MINEADADGD GTISFSEFVC 250
    VMTGKMIDTQ SKKETYRVVN QGQGQVQRHT RNDRAGGTNW ERDIAVGVAS 300
    NIIASPISDF MKDRFKDLFE ALLS 324
    Length:324
    Mass (Da):36,807
    Last modified:November 2, 2001 - v3
    Checksum:i94277BFBC2D2C5CE
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti223 – 2264QTKA → KQKL in AAC37419. (PubMed:2302732)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L34546 Genomic DNA. Translation: AAC37419.1.
    D45848 Genomic DNA. Translation: BAA08282.1.
    AC004261 Genomic DNA. Translation: AAD12001.1.
    CP002685 Genomic DNA. Translation: AEC09928.1.
    CP002685 Genomic DNA. Translation: AEC09931.1.
    AF424577 mRNA. Translation: AAL11571.1.
    AY120719 mRNA. Translation: AAM53277.1.
    BT000036 mRNA. Translation: AAN15355.1.
    PIRiB34669.
    T02109.
    RefSeqiNP_001189723.1. NM_001202794.1. [P25071-1]
    NP_181643.1. NM_129675.4. [P25071-1]
    UniGeneiAt.12547.
    At.269.

    Genome annotation databases

    EnsemblPlantsiAT2G41100.1; AT2G41100.1; AT2G41100. [P25071-1]
    AT2G41100.4; AT2G41100.4; AT2G41100. [P25071-1]
    GeneIDi818709.
    KEGGiath:AT2G41100.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L34546 Genomic DNA. Translation: AAC37419.1 .
    D45848 Genomic DNA. Translation: BAA08282.1 .
    AC004261 Genomic DNA. Translation: AAD12001.1 .
    CP002685 Genomic DNA. Translation: AEC09928.1 .
    CP002685 Genomic DNA. Translation: AEC09931.1 .
    AF424577 mRNA. Translation: AAL11571.1 .
    AY120719 mRNA. Translation: AAM53277.1 .
    BT000036 mRNA. Translation: AAN15355.1 .
    PIRi B34669.
    T02109.
    RefSeqi NP_001189723.1. NM_001202794.1. [P25071-1 ]
    NP_181643.1. NM_129675.4. [P25071-1 ]
    UniGenei At.12547.
    At.269.

    3D structure databases

    ProteinModelPortali P25071.
    SMRi P25071. Positions 2-312.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 4046. 3 interactions.
    IntActi P25071. 89 interactions.
    STRINGi 3702.AT2G41100.1-P.

    Proteomic databases

    PaxDbi P25071.
    PRIDEi P25071.

    Protocols and materials databases

    DNASUi 818709.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G41100.1 ; AT2G41100.1 ; AT2G41100 . [P25071-1 ]
    AT2G41100.4 ; AT2G41100.4 ; AT2G41100 . [P25071-1 ]
    GeneIDi 818709.
    KEGGi ath:AT2G41100.

    Organism-specific databases

    TAIRi AT2G41100.

    Phylogenomic databases

    eggNOGi COG5126.
    HOGENOMi HOG000233018.
    InParanoidi P25071.
    KOi K13448.
    OMAi MINEADA.
    PhylomeDBi P25071.

    Gene expression databases

    ArrayExpressi P25071.
    Genevestigatori P25071.

    Family and domain databases

    Gene3Di 1.10.238.10. 2 hits.
    InterProi IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    [Graphical view ]
    Pfami PF00036. EF-hand_1. 4 hits.
    PF13499. EF-hand_7. 1 hit.
    [Graphical view ]
    SMARTi SM00054. EFh. 6 hits.
    [Graphical view ]
    PROSITEi PS00018. EF_HAND_1. 6 hits.
    PS50222. EF_HAND_2. 6 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Rain-, wind-, and touch-induced expression of calmodulin and calmodulin-related genes in Arabidopsis."
      Braam J., Davis R.W.
      Cell 60:357-364(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Columbia.
    2. "Arabidopsis TCH3 encodes a novel Ca2+ binding protein and shows environmentally induced and tissue-specific regulation."
      Sistrunk M.L., Antosiewicz D.M., Purugganan M.M., Braam J.
      Plant Cell 6:1553-1565(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: SEQUENCE REVISION.
    3. "Touch-inducible genes for calmodulin and a calmodulin-related protein are located in tandem on a chromosome of Arabidopsis thaliana."
      Ito T., Hirano M., Akama K., Shimura Y., Okada K.
      Plant Cell Physiol. 36:1369-1373(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Landsberg erecta.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    5. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    7. "Calmodulins and related potential calcium sensors of Arabidopsis."
      McCormack E., Braam J.
      New Phytol. 159:585-598(2003)
      Cited for: GENE FAMILY, NOMENCLATURE.
    8. "PINOID-mediated signaling involves calcium-binding proteins."
      Benjamins R., Ampudia C.S., Hooykaas P.J., Offringa R.
      Plant Physiol. 132:1623-1630(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PID, INDUCTION BY AUXIN.
    9. "Genome-wide identification of touch- and darkness-regulated Arabidopsis genes: a focus on calmodulin-like and XTH genes."
      Lee D., Polisensky D.H., Braam J.
      New Phytol. 165:429-444(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.

    Entry informationi

    Entry nameiCML12_ARATH
    AccessioniPrimary (citable) accession number: P25071
    Secondary accession number(s): Q38972, Q39064, Q8H1A0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: November 2, 2001
    Last modified: October 1, 2014
    This is version 123 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3