P25059 (POL_BLVAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pol polyprotein

Cleaved into the following 2 chains:

1. Reverse transcriptase/ribonuclease H
   Short name=RT
   EC=2.7.7.49
   EC=2.7.7.7
   EC=3.1.26.4
2. Integrase
   Short name=IN

Gene names

Name:

pol

Organism

Bovine leukemia virus (isolate Australian) (BLV)

Taxonomic identifier

11903 [NCBI]

Taxonomic lineage

Viruses › Retro-transcribing viruses › Retroviridae › Orthoretrovirinae › Deltaretrovirus

Virus host

Bos taurus (Bovine) [TaxID: 9913]

Protein attributes

Sequence length	852 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). Endonucleolytic cleavage to 5'-phosphomonoester.
Post-translational modification	Specific enzymatic cleavages in vivo yield mature proteins.
Sequence similarities	Belongs to the retroviral Pol polyprotein family. Contains 1 integrase catalytic domain. Contains 1 integrase-type DNA-binding domain. Contains 1 reverse transcriptase domain. Contains 1 RNase H domain.

Ontologies

Keywords
Biological process	DNA integration DNA recombination Initiation of viral infection Viral genome integration
Ligand	DNA-binding
Molecular function	Endonuclease Hydrolase Nuclease Nucleotidyltransferase RNA-directed DNA polymerase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	DNA integration Inferred from electronic annotation. Source: UniProtKB-KW DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW RNA-dependent DNA replication Inferred from electronic annotation. Source: InterPro
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: EC RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease H activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 852	852	Pol polyprotein		PRO_0000125481
Regions
	Domain	22 – 212	191	Reverse transcriptase
	Domain	432 – 562	131	RNase H
	Domain	615 – 779	165	Integrase catalytic
	DNA binding	788 – 836	49	Integrase-type

Sequences

	Sequence		Length	Mass (Da)
	P25059 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: CD69FE59E1208A08	FASTA	852	95,441
	10 20 30 40 50 60 GTSIPFKLER LQALQDLVHR SLEAGYISPW DGPGNNPVFP VRKPNGAWRF VHDLRVTNAL 70 80 90 100 110 120 TKPIPALSPG PPDLTAIPTH LPHIICLDLK DAFFQIPVED RFRSYFAFTL PTPGGLQPHR 130 140 150 160 170 180 RFAWRVLPQG FINSPALFER ALQEPLRQVS AAFSQSLLVS YMDDILYVSP TEEQRLQCYQ 190 200 210 220 230 240 TMAAHLRDLG FQVASEKTRQ TPSPVPFLGQ MVHERMVTYQ SLPTLQISSP ISLHQLQTVL 250 260 270 280 290 300 GDLQWVSRGT PTTRRPLQLL YSSLKGIDDP RAIIHLSPEQ QQGIAELRQA LSHNARSRYN 310 320 330 340 350 360 EQEPLLAYVH LTRAGSTLVL FQKGAQFPLA YFQTPLTDNQ ASPWGLLLLL GCQYLQAQAL 370 380 390 400 410 420 SSYAKTILKY YHNLPKTSLD NWIQSSEDPR VQELLQLWPQ ISSQGIQPPG PWKTLVTRAE 430 440 450 460 470 480 VFLTPQFSPE PIPAALCLFS DGAARRGAYC LWKDHLLDFQ AVPAPESAQK GELAGLLAGL 490 500 510 520 530 540 AAAPPEPLNI WVDSKYLYSL LRTLVLGAWL QPDPVPSYAL LYKSLLRHPA IFVGHVRSHS 550 560 570 580 590 600 SASHPIASLN NYVDQLLPLE TPEQWHKLTH CNSRALSRWP NPRISAWDPR SPATLCETCQ 610 620 630 640 650 660 RLNPTGGGKM RTIQRGWAPN HIWQADITHY KYKQFTYALH VFVDTYSGAT HASAKRGLTT 670 680 690 700 710 720 QTTIEGLLEA IVHLGRPKKL NTDQGANYTS KTFVRFCQQF GISLSHHVPY NPTSSGLVER 730 740 750 760 770 780 TNGLLKLLLS KYHLDEPHLP MTQALSRALW THNQINLLPI LKTRWELHHS PPLAVISEGG 790 800 810 820 830 840 ETPKGSDKLF LYKLPGQNNR RWLGPLPALV EASGGALLAT NPPVWVPWRL LKAFKCLKND 850 GPEDAPNRSS DG

References

[1]

"Molecular cloning and sequencing of an Australian isolate of proviral bovine leukaemia virus DNA: comparison with other isolates." Coulston J., Naif H., Brandon R., Kumar S., Khan S., Daniel R.C.W., Lavin M.F. J. Gen. Virol. 71:1737-1746(1990) [PubMed: 2167927] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	D00647 Genomic DNA. Translation: BAA00544.1.
PIR	GNLJGA. JQ0555.
3D structure databases
ProteinModelPortal	P25059.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001037. Integrase_C_retrovir. IPR001584. Integrase_cat-core. IPR012337. RNaseH-like_dom. IPR002156. RNaseH_domain. IPR000477. RVT. [Graphical view]
Pfam	PF00552. IN_DBD_C. 1 hit. PF00075. RNase_H. 1 hit. PF00665. rve. 1 hit. PF00078. RVT_1. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
PROSITE	PS50994. INTEGRASE. 1 hit. PS51027. INTEGRASE_DBD. 1 hit. PS50879. RNASE_H. 1 hit. PS50878. RT_POL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

POL_BLVAU

Accession

Primary (citable) accession number: P25059

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	December 14, 2011
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program