Reviewed, UniProtKB/Swiss-Prot P25055 (PGKA_CRIFA)

Names and origin

Protein names

Recommended name:
    Phosphoglycerate kinase, glycosomal
      Short name=Phosphoglycerate kinase A
    EC=2.7.2.3

Gene names

Name:	PGKA
Synonyms:	PGK-A

Organism

Crithidia fasciculata

Taxonomic identifier

5656 [NCBI]

Taxonomic lineage

Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Crithidia

Protein attributes

Sequence length	505 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.
Subunit structure	Monomer.
Subcellular location	Glycosome.
Sequence similarities	Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Glycosome Peroxisome
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	glycosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycerate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 505	505	Phosphoglycerate kinase, glycosomal		PRO_0000145848
Regions
	Nucleotide binding	460 – 463	4	ATP By similarity
	Region	30 – 32	3	Substrate binding By similarity
	Region	68 – 71	4	Substrate binding By similarity
Sites
	Binding site	45	1	Substrate By similarity
	Binding site	220	1	Substrate By similarity
	Binding site	257	1	Substrate By similarity
	Binding site	308	1	ATP By similarity
	Binding site	399	1	ATP; via carbonyl oxygen By similarity
	Binding site	430	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P25055-1 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: E184277AAB86D355	FASTA	505	54,758
	10 20 30 40 50 60 MLLSTQSIPI KKSVDDIWPV AGKRVLIRVD FNVPTSSGGI DDDFRIRSAI PTIRRIVDQG 70 80 90 100 110 120 GICILISHLG RPTGVDYDTA HAEQEKRQTP VQLPNSRGKT AFFSGLRGDA KATILAWSSQ 130 140 150 160 170 180 REKAATLSNP YGSGKTAVFA RLPEEEKRRL LLRFMSEHKE ELFPQLGSAG YEKEFSLEPV 190 200 210 220 230 240 AVKLAELLDQ HVYFGHDCLG AQADIAKLRC GEVMLLENLR FYTNENSSDE RKRMLMARIL 250 260 270 280 290 300 ASYADVYIND AFGTAHRDSA SLTGIPRVLQ QGAAGYLMEK EISYFSKVLN NPPRPLLAII 310 320 330 340 350 360 GGAKLSDKMQ VLENLLDCVD SLFIGGGLAY TFLRSQGYSI GTSHFEEAFV PFAQALPLAG 370 380 390 400 410 420 SGRQVRVVLP EDHVCHAHTR PAEAPLTTTS ANIPDGYAGL DIGPLTIQSI THLVRQCSSV 430 440 450 460 470 480 IWNGPLGMFE MPYYAFGTFS IARVVSQSSA AKGTTSIVGG GDTASAMMKS GEAAHISHIS 490 500 TGGNASLELL EGKVLAAVAV LDNKE

References

[1]	"A phosphoglycerate kinase-related gene conserved between Trypanosoma brucei and Crithidia fasciculata." Swinkels B.W., Loiseau A., Opperdoes F.R., Borst P.A. Mol. Biochem. Parasitol. 50:69-78(1992) [PubMed: 1542317] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X17251 Genomic DNA. Translation: CAA35114.1.
PIR	KICRGF. S14463.
3D structure databases
SMR	P25055. Positions 11-504.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.2.3. 571.
Family and domain databases
InterPro	IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015911. Phosphoglycerate_kinase_CS. IPR015824. Phosphoglycerate_kinase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.1270. Phosphoglycerate_kinase_C. 1 hit. G3DSA:3.40.50.1260. Phosphoglycerate_kinase_N. 1 hit.
PANTHER	PTHR11406. PGK. 1 hit.
Pfam	PF00162. PGK. 1 hit. [Graphical view]
PRINTS	PR00477. PHGLYCKINASE.
PROSITE	PS00111. PGLYCERATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGKA_CRIFA

Accession

Primary (citable) accession number: P25055

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	February 9, 2010
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)