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Protein

Phosphoglycerate kinase, glycosomal

Gene

PGKA

Organism
Crithidia fasciculata
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

Pathway:iglycolysis

This protein is involved in step 2 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase, glycosomal (GAPDG)
  2. Phosphoglycerate kinase, glycosomal (PGKA), Phosphoglycerate kinase, glycosomal (PGKC), Phosphoglycerate kinase, cytosolic (PGKB)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei45 – 451SubstrateBy similarity
Binding sitei220 – 2201SubstrateBy similarity
Binding sitei257 – 2571SubstrateBy similarity
Binding sitei308 – 3081ATPBy similarity
Binding sitei399 – 3991ATP; via carbonyl oxygenBy similarity
Binding sitei430 – 4301ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi460 – 4634ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00185.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinase, glycosomal (EC:2.7.2.3)
Short name:
Phosphoglycerate kinase A
Gene namesi
Name:PGKA
Synonyms:PGK-A
OrganismiCrithidia fasciculata
Taxonomic identifieri5656 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeLeishmaniinaeCrithidia

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Glycosome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 505505Phosphoglycerate kinase, glycosomalPRO_0000145848Add
BLAST

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP25055.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni30 – 323Substrate bindingBy similarity
Regioni68 – 714Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.Curated

Family and domain databases

Gene3Di3.40.50.1260. 2 hits.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase.
InterProiIPR027250. Pgk_euglenozoa.
IPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PIRSF500126. Pgk_euglenozoa. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 2 hits.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25055-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLSTQSIPI KKSVDDIWPV AGKRVLIRVD FNVPTSSGGI DDDFRIRSAI
60 70 80 90 100
PTIRRIVDQG GICILISHLG RPTGVDYDTA HAEQEKRQTP VQLPNSRGKT
110 120 130 140 150
AFFSGLRGDA KATILAWSSQ REKAATLSNP YGSGKTAVFA RLPEEEKRRL
160 170 180 190 200
LLRFMSEHKE ELFPQLGSAG YEKEFSLEPV AVKLAELLDQ HVYFGHDCLG
210 220 230 240 250
AQADIAKLRC GEVMLLENLR FYTNENSSDE RKRMLMARIL ASYADVYIND
260 270 280 290 300
AFGTAHRDSA SLTGIPRVLQ QGAAGYLMEK EISYFSKVLN NPPRPLLAII
310 320 330 340 350
GGAKLSDKMQ VLENLLDCVD SLFIGGGLAY TFLRSQGYSI GTSHFEEAFV
360 370 380 390 400
PFAQALPLAG SGRQVRVVLP EDHVCHAHTR PAEAPLTTTS ANIPDGYAGL
410 420 430 440 450
DIGPLTIQSI THLVRQCSSV IWNGPLGMFE MPYYAFGTFS IARVVSQSSA
460 470 480 490 500
AKGTTSIVGG GDTASAMMKS GEAAHISHIS TGGNASLELL EGKVLAAVAV

LDNKE
Length:505
Mass (Da):54,758
Last modified:May 1, 1992 - v1
Checksum:iE184277AAB86D355
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17251 Genomic DNA. Translation: CAA35114.1.
PIRiS14463. KICRGF.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17251 Genomic DNA. Translation: CAA35114.1.
PIRiS14463. KICRGF.

3D structure databases

ProteinModelPortaliP25055.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00109; UER00185.

Family and domain databases

Gene3Di3.40.50.1260. 2 hits.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase.
InterProiIPR027250. Pgk_euglenozoa.
IPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PIRSF500126. Pgk_euglenozoa. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 2 hits.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A phosphoglycerate kinase-related gene conserved between Trypanosoma brucei and Crithidia fasciculata."
    Swinkels B.W., Loiseau A., Opperdoes F.R., Borst P.A.
    Mol. Biochem. Parasitol. 50:69-78(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiPGKA_CRIFA
AccessioniPrimary (citable) accession number: P25055
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: January 7, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.