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Protein

Thiazole tautomerase

Gene

tenI

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the irreversible aromatization of 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate (cThz*-P) to 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate (cThz-P), a step in the biosynthesis of the thiazole phosphate moiety of thiamine. Cannot use cThz* as substrate, indicating that the phosphate group is essential. Has no thiamine phosphate synthase activity, despite a high sequence similarity with ThiE.1 Publication

Caution

Was originally described as a regulatory protein involved in the regulation of the production of extracellular enzymes.1 PublicationCurated

Catalytic activityi

2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate = 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate.1 Publication

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.1 Publication
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei102Substrate1 Publication1
Active sitei122Proton acceptor1 Publication1
Binding sitei156Substrate; via amide nitrogen1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processThiamine biosynthesis

Enzyme and pathway databases

BioCyciBSUB:BSU11660-MONOMER
MetaCyc:BSU11660-MONOMER
UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Thiazole tautomerase1 Publication (EC:5.3.99.101 Publication)
Gene namesi
Name:tenI1 Publication
Ordered Locus Names:BSU11660
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Disruption phenotypei

Inactivation of this gene causes a delay in sporulation, but does not affect cell growth and the production of extracellular enzymes.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi122H → Q or A: Loss of enzymatic activity. 1 Publication1

Chemistry databases

DrugBankiDB03570 Tris-Hydroxymethyl-Methyl-Ammonium

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001570871 – 205Thiazole tautomeraseAdd BLAST205

Proteomic databases

PaxDbiP25053
PRIDEiP25053

Expressioni

Inductioni

Strongly repressed by thiamine.1 Publication

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100006446

Structurei

Secondary structure

1205
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 6Combined sources5
Helixi13 – 23Combined sources11
Helixi24 – 26Combined sources3
Beta strandi28 – 32Combined sources5
Helixi39 – 51Combined sources13
Helixi56 – 58Combined sources3
Beta strandi59 – 63Combined sources5
Helixi65 – 69Combined sources5
Turni70 – 72Combined sources3
Beta strandi75 – 78Combined sources4
Helixi85 – 91Combined sources7
Beta strandi96 – 101Combined sources6
Helixi104 – 112Combined sources9
Beta strandi116 – 121Combined sources6
Helixi136 – 146Combined sources11
Beta strandi151 – 156Combined sources6
Helixi159 – 161Combined sources3
Helixi162 – 167Combined sources6
Beta strandi171 – 176Combined sources6
Helixi177 – 180Combined sources4
Beta strandi182 – 184Combined sources3
Helixi185 – 199Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YADX-ray2.10A/B/C/D1-205[»]
3QH2X-ray2.23A/B/C/D1-205[»]
ProteinModelPortaliP25053
SMRiP25053
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25053

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni176 – 177Substrate binding1 Publication2

Sequence similaritiesi

Belongs to the thiazole tautomerase family.Curated

Phylogenomic databases

eggNOGiENOG4108VEU Bacteria
COG0352 LUCA
HOGENOMiHOG000155781
InParanoidiP25053
KOiK10810
OMAiVDFIHIR
PhylomeDBiP25053

Family and domain databases

CDDicd00564 TMP_TenI, 1 hit
Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR036206 ThiamineP_synth_sf
IPR022998 ThiamineP_synth_TenI
PfamiView protein in Pfam
PF02581 TMP-TENI, 1 hit
SUPFAMiSSF51391 SSF51391, 1 hit

Sequencei

Sequence statusi: Complete.

P25053-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELHAITDDS KPVEELARII ITIQNEVDFI HIRERSKSAA DILKLLDLIF
60 70 80 90 100
EGGIDKRKLV MNGRVDIALF STIHRVQLPS GSFSPKQIRA RFPHLHIGRS
110 120 130 140 150
VHSLEEAVQA EKEDADYVLF GHVFETDCKK GLEGRGVSLL SDIKQRISIP
160 170 180 190 200
VIAIGGMTPD RLRDVKQAGA DGIAVMSGIF SSAEPLEAAR RYSRKLKEMR

YEKAL
Length:205
Mass (Da):22,929
Last modified:May 1, 1992 - v1
Checksum:iB04749ED70F0A088
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73546 Genomic DNA Translation: AAA22849.1
AL009126 Genomic DNA Translation: CAB13023.1
PIRiB39184 XMBSTI
RefSeqiNP_389048.1, NC_000964.3
WP_003232908.1, NZ_JNCM01000035.1

Genome annotation databases

EnsemblBacteriaiCAB13023; CAB13023; BSU11660
GeneIDi936411
KEGGibsu:BSU11660
PATRICifig|224308.179.peg.1255

Similar proteinsi

Entry informationi

Entry nameiTENI_BACSU
AccessioniPrimary (citable) accession number: P25053
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: May 23, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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