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Protein

Thiazole tautomerase

Gene

tenI

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the irreversible aromatization of 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate (cThz*-P) to 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate (cThz-P), a step in the biosynthesis of the thiazole phosphate moiety of thiamine. Cannot use cThz* as substrate, indicating that the phosphate group is essential. Has no thiamine phosphate synthase activity, despite a high sequence similarity with ThiE.1 Publication

Catalytic activityi

2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate = 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate.1 Publication

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.1 Publication
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei102 – 1021Substrate1 Publication
Active sitei122 – 1221Proton acceptor1 Publication
Binding sitei156 – 1561Substrate; via amide nitrogen1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Thiamine biosynthesis

Enzyme and pathway databases

BioCyciBSUB:BSU11660-MONOMER.
MetaCyc:BSU11660-MONOMER.
UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiazole tautomerase1 Publication (EC:5.3.99.101 Publication)
Gene namesi
Name:tenI1 Publication
Ordered Locus Names:BSU11660
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Inactivation of this gene causes a delay in sporulation, but does not affect cell growth and the production of extracellular enzymes.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi122 – 1221H → Q or A: Loss of enzymatic activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 205205Thiazole tautomerasePRO_0000157087Add
BLAST

Proteomic databases

PaxDbiP25053.

Expressioni

Inductioni

Strongly repressed by thiamine.1 Publication

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100006446.

Structurei

Secondary structure

1
205
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 65Combined sources
Helixi13 – 2311Combined sources
Helixi24 – 263Combined sources
Beta strandi28 – 325Combined sources
Helixi39 – 5113Combined sources
Helixi56 – 583Combined sources
Beta strandi59 – 635Combined sources
Helixi65 – 695Combined sources
Turni70 – 723Combined sources
Beta strandi75 – 784Combined sources
Helixi85 – 917Combined sources
Beta strandi96 – 1016Combined sources
Helixi104 – 1129Combined sources
Beta strandi116 – 1216Combined sources
Helixi136 – 14611Combined sources
Beta strandi151 – 1566Combined sources
Helixi159 – 1613Combined sources
Helixi162 – 1676Combined sources
Beta strandi171 – 1766Combined sources
Helixi177 – 1804Combined sources
Beta strandi182 – 1843Combined sources
Helixi185 – 19915Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YADX-ray2.10A/B/C/D1-205[»]
3QH2X-ray2.23A/B/C/D1-205[»]
ProteinModelPortaliP25053.
SMRiP25053. Positions 1-200.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25053.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni176 – 1772Substrate binding1 Publication

Sequence similaritiesi

Belongs to the thiazole tautomerase family.Curated

Phylogenomic databases

eggNOGiENOG4108VEU. Bacteria.
COG0352. LUCA.
HOGENOMiHOG000155781.
InParanoidiP25053.
KOiK10810.
OMAiVDFIHIR.
OrthoDBiEOG6W19NW.
PhylomeDBiP25053.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR022998. ThiaminP_synth_SF.
IPR003733. TMP_synthase.
[Graphical view]
PfamiPF02581. TMP-TENI. 1 hit.
[Graphical view]
SUPFAMiSSF51391. SSF51391. 1 hit.

Sequencei

Sequence statusi: Complete.

P25053-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELHAITDDS KPVEELARII ITIQNEVDFI HIRERSKSAA DILKLLDLIF
60 70 80 90 100
EGGIDKRKLV MNGRVDIALF STIHRVQLPS GSFSPKQIRA RFPHLHIGRS
110 120 130 140 150
VHSLEEAVQA EKEDADYVLF GHVFETDCKK GLEGRGVSLL SDIKQRISIP
160 170 180 190 200
VIAIGGMTPD RLRDVKQAGA DGIAVMSGIF SSAEPLEAAR RYSRKLKEMR

YEKAL
Length:205
Mass (Da):22,929
Last modified:May 1, 1992 - v1
Checksum:iB04749ED70F0A088
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73546 Genomic DNA. Translation: AAA22849.1.
AL009126 Genomic DNA. Translation: CAB13023.1.
PIRiB39184. XMBSTI.
RefSeqiNP_389048.1. NC_000964.3.
WP_003232908.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB13023; CAB13023; BSU11660.
GeneIDi936411.
KEGGibsu:BSU11660.
PATRICi18974049. VBIBacSub10457_1218.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73546 Genomic DNA. Translation: AAA22849.1.
AL009126 Genomic DNA. Translation: CAB13023.1.
PIRiB39184. XMBSTI.
RefSeqiNP_389048.1. NC_000964.3.
WP_003232908.1. NZ_JNCM01000035.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YADX-ray2.10A/B/C/D1-205[»]
3QH2X-ray2.23A/B/C/D1-205[»]
ProteinModelPortaliP25053.
SMRiP25053. Positions 1-200.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100006446.

Proteomic databases

PaxDbiP25053.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13023; CAB13023; BSU11660.
GeneIDi936411.
KEGGibsu:BSU11660.
PATRICi18974049. VBIBacSub10457_1218.

Phylogenomic databases

eggNOGiENOG4108VEU. Bacteria.
COG0352. LUCA.
HOGENOMiHOG000155781.
InParanoidiP25053.
KOiK10810.
OMAiVDFIHIR.
OrthoDBiEOG6W19NW.
PhylomeDBiP25053.

Enzyme and pathway databases

UniPathwayiUPA00060.
BioCyciBSUB:BSU11660-MONOMER.
MetaCyc:BSU11660-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP25053.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR022998. ThiaminP_synth_SF.
IPR003733. TMP_synthase.
[Graphical view]
PfamiPF02581. TMP-TENI. 1 hit.
[Graphical view]
SUPFAMiSSF51391. SSF51391. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of a pair of novel genes that regulate production of extracellular enzymes in Bacillus subtilis."
    Pang A.S.-H., Nathoo S., Wong S.-L.
    J. Bacteriol. 173:46-54(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  3. "RNA expression analysis using an antisense Bacillus subtilis genome array."
    Lee J.M., Zhang S., Saha S., Santa Anna S., Jiang C., Perkins J.
    J. Bacteriol. 183:7371-7380(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  4. "Structural characterization of the regulatory proteins TenA and TenI from Bacillus subtilis and identification of TenA as a thiaminase II."
    Toms A.V., Haas A.L., Park J.-H., Begley T.P., Ealick S.E.
    Biochemistry 44:2319-2329(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS), SUBUNIT.
  5. "A missing enzyme in thiamin thiazole biosynthesis: identification of TenI as a thiazole tautomerase."
    Hazra A.B., Han Y., Chatterjee A., Zhang Y., Lai R.Y., Ealick S.E., Begley T.P.
    J. Am. Chem. Soc. 133:9311-9319(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.23 ANGSTROMS) IN COMPLEX WITH REACTION PRODUCT, FUNCTION, CATALYTIC ACTIVITY, REACTION MECHANISM, ACTIVE SITE, MUTAGENESIS OF HIS-122.

Entry informationi

Entry nameiTENI_BACSU
AccessioniPrimary (citable) accession number: P25053
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: February 17, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally described as a regulatory protein involved in the regulation of the production of extracellular enzymes.1 PublicationCurated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.