P25053 (TENI_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Regulatory protein tenI

Gene names

Name:	tenI
Ordered Locus Names:	BSU11660

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	205 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Reduces the stimulatory effect of the tenA transcriptional activator on production of certain extracellular enzymes. Is not an essential protein, but affects the sporulation frequency.
Pathway	Cofactor metabolism; thiamine degradation. [regulation]
Subunit structure	Dimer of dimers.
Induction	Repressed by thiamine.
Sequence similarities	Belongs to the TMP-PPase family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Molecular function	Repressor
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW thiamine biosynthetic process Inferred from electronic annotation. Source: InterPro transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	thiamine-phosphate diphosphorylase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 205

205

Regulatory protein tenI

PRO_0000157087

Secondary structure

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205

Helix Strand Turn

Details...

Beta strand

2 – 6

5

Helix

13 – 23

11

Helix

24 – 26

3

Beta strand

28 – 32

5

Helix

39 – 51

13

Helix

56 – 58

3

Beta strand

59 – 63

5

Helix

65 – 69

5

Turn

70 – 72

3

Beta strand

75 – 78

4

Helix

85 – 91

7

Beta strand

96 – 101

6

Helix

104 – 112

9

Beta strand

116 – 121

6

Helix

136 – 146

11

Beta strand

151 – 156

6

Helix

159 – 161

3

Helix

162 – 167

6

Beta strand

171 – 176

6

Helix

177 – 180

4

Beta strand

182 – 184

3

Helix

185 – 199

15

Sequences

	Sequence		Length	Mass (Da)
	P25053 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: B04749ED70F0A088	FASTA	205	22,929
	10 20 30 40 50 60 MELHAITDDS KPVEELARII ITIQNEVDFI HIRERSKSAA DILKLLDLIF EGGIDKRKLV 70 80 90 100 110 120 MNGRVDIALF STIHRVQLPS GSFSPKQIRA RFPHLHIGRS VHSLEEAVQA EKEDADYVLF 130 140 150 160 170 180 GHVFETDCKK GLEGRGVSLL SDIKQRISIP VIAIGGMTPD RLRDVKQAGA DGIAVMSGIF 190 200 SSAEPLEAAR RYSRKLKEMR YEKAL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of a pair of novel genes that regulate production of extracellular enzymes in Bacillus subtilis." Pang A.S.-H., Nathoo S., Wong S.-L. J. Bacteriol. 173:46-54(1991) [PubMed: 1898926] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"Structural characterization of the regulatory proteins TenA and TenI from Bacillus subtilis and identification of TenA as a thiaminase II." Toms A.V., Haas A.L., Park J.-H., Begley T.P., Ealick S.E. Biochemistry 44:2319-2329(2005) [PubMed: 15709744] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF APOPROTEIN.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M73546 Genomic DNA. Translation: AAA22849.1.
AL009126 Genomic DNA. Translation: CAB13023.1.

PIR

XMBSTI. B39184.

RefSeq

NP_389048.1. NC_000964.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1YAD	X-ray	2.10	A/B/C/D	1-205	[»]
3QH2	X-ray	2.23	A/B/C/D	1-205	[»]

ProteinModelPortal

P25053.

SMR

P25053. Positions 1-200.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBBACT00000001742; EBBACP00000001742; EBBACG00000001740.

GeneID

936411.

GenomeReviews

Gene locus BSU11660 in contig AL009126_GR.

KEGG

bsu:BSU11660.

NMPDR

fig|224308.1.peg.1167.

PATRIC

18974049. VBIBacSub10457_1218.

Organism-specific databases

GenoList

BSU11660. [Micado]

Phylogenomic databases

GeneTree

EBGT00050000001882.

HOGENOM

HBG754477.

OMA

ELVNVAM.

PhylomeDB

P25053.

ProtClustDB

PRK07695.

Enzyme and pathway databases

BioCyc

BSUB:BSU11660-MONOMER.

Family and domain databases

InterPro

IPR013785. Aldolase_TIM.
IPR022998. ThiaminP_synth_SF.
IPR003733. TMP_synthase.
[Graphical view]

Gene3D

G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.

KO

K10810.

Pfam

PF02581. TMP-TENI. 1 hit.
[Graphical view]

SUPFAM

SSF51391. TMP_synthase. 1 hit.

ProtoNet

Search...

Entry information

Entry name

TENI_BACSU

Accession

Primary (citable) accession number: P25053

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	January 25, 2012
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program