Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Vancomycin/teicoplanin A-type resistance protein VanA

Gene

vanA

Organism
Enterococcus faecium (Streptococcus faecium)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for high-level resistance to glycopeptide antibiotics. D-Ala--D-Ala ligase of altered specificity which catalyzes ester bond formation between D-Ala and various D-hydroxy acids; produces a peptidoglycan which does not terminate in D-alanine but in D-lactate, thus preventing vancomycin or teicoplanin binding.1 Publication

Catalytic activityi

D-alanine + (R)-lactate + ATP = D-alanyl-(R)-lactate + ADP + phosphate.1 Publication

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Kineticsi

  1. KM=7.1 mM for D-lactate1 Publication
  2. KM=3.8 mM for D-alanine1 Publication
  3. KM=0.6 mM for 2-hydroxybutyrate1 Publication
  4. KM=3.2 mM for 2-hydroxyvalerate1 Publication
  5. KM=11 mM for 2-hydroxycaproate1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei133ATP1
    Binding sitei241ATP; via amide nitrogen1
    Binding sitei244Substrate1
    Metal bindingi305Magnesium 11 Publication1
    Metal bindingi305Magnesium 21 Publication1
    Metal bindingi307Magnesium 21 Publication1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi169 – 171ATP3
    Nucleotide bindingi177 – 178ATP2
    Nucleotide bindingi207 – 214ATP8
    Nucleotide bindingi304 – 305ATP2

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Antibiotic resistance, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-15466.
    BRENDAi6.1.2.1. 2096.
    SABIO-RKP25051.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vancomycin/teicoplanin A-type resistance protein VanA (EC:6.1.2.1)
    Alternative name(s):
    D-alanine--D-lactate ligase
    VanA ligase
    Gene namesi
    Name:vanA
    Encoded oniPlasmid pIP8160 Publication
    OrganismiEnterococcus faecium (Streptococcus faecium)
    Taxonomic identifieri1352 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Chemistry databases

    ChEMBLiCHEMBL6037.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001779171 – 343Vancomycin/teicoplanin A-type resistance protein VanAAdd BLAST343

    Expressioni

    Inductioni

    By vancomycin, mediated by VanS/VanR.

    Interactioni

    Chemistry databases

    BindingDBiP25051.

    Structurei

    Secondary structure

    1343
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi4 – 11Combined sources8
    Helixi17 – 30Combined sources14
    Turni33 – 35Combined sources3
    Beta strandi36 – 43Combined sources8
    Beta strandi49 – 53Combined sources5
    Beta strandi65 – 69Combined sources5
    Turni73 – 75Combined sources3
    Beta strandi77 – 82Combined sources6
    Beta strandi85 – 90Combined sources6
    Beta strandi92 – 96Combined sources5
    Turni101 – 103Combined sources3
    Beta strandi104 – 106Combined sources3
    Helixi107 – 115Combined sources9
    Beta strandi119 – 121Combined sources3
    Helixi124 – 131Combined sources8
    Helixi133 – 142Combined sources10
    Beta strandi150 – 153Combined sources4
    Helixi161 – 163Combined sources3
    Beta strandi168 – 174Combined sources7
    Turni177 – 180Combined sources4
    Beta strandi182 – 184Combined sources3
    Helixi187 – 189Combined sources3
    Helixi190 – 197Combined sources8
    Turni198 – 200Combined sources3
    Beta strandi202 – 208Combined sources7
    Beta strandi212 – 222Combined sources11
    Beta strandi232 – 239Combined sources8
    Helixi243 – 245Combined sources3
    Beta strandi246 – 248Combined sources3
    Helixi249 – 251Combined sources3
    Beta strandi254 – 258Combined sources5
    Helixi266 – 282Combined sources17
    Beta strandi286 – 295Combined sources10
    Beta strandi301 – 309Combined sources9
    Helixi317 – 324Combined sources8
    Helixi329 – 341Combined sources13

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1E4EX-ray2.50A/B1-343[»]
    ProteinModelPortaliP25051.
    SMRiP25051.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP25051.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini137 – 338ATP-graspAdd BLAST202

    Sequence similaritiesi

    Belongs to the D-alanine--D-alanine ligase family.Curated
    Contains 1 ATP-grasp domain.Curated

    Phylogenomic databases

    KOiK15739.

    Family and domain databases

    Gene3Di3.30.1490.20. 1 hit.
    3.30.470.20. 2 hits.
    3.40.50.20. 1 hit.
    HAMAPiMF_00047. Dala_Dala_lig. 1 hit.
    InterProiIPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR000291. D-Ala_lig_Van_CS.
    IPR005905. D_ala_D_ala.
    IPR011095. Dala_Dala_lig_C.
    IPR011127. Dala_Dala_lig_N.
    IPR016185. PreATP-grasp_dom.
    [Graphical view]
    PfamiPF07478. Dala_Dala_lig_C. 1 hit.
    PF01820. Dala_Dala_lig_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF039102. Ddl/VanB. 1 hit.
    SUPFAMiSSF52440. SSF52440. 1 hit.
    TIGRFAMsiTIGR01205. D_ala_D_alaTIGR. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 1 hit.
    PS00843. DALA_DALA_LIGASE_1. 1 hit.
    PS00844. DALA_DALA_LIGASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P25051-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MNRIKVAILF GGCSEEHDVS VKSAIEIAAN INKEKYEPLY IGITKSGVWK
    60 70 80 90 100
    MCEKPCAEWE NDNCYSAVLS PDKKMHGLLV KKNHEYEINH VDVAFSALHG
    110 120 130 140 150
    KSGEDGSIQG LFELSGIPFV GCDIQSSAIC MDKSLTYIVA KNAGIATPAF
    160 170 180 190 200
    WVINKDDRPV AATFTYPVFV KPARSGSSFG VKKVNSADEL DYAIESARQY
    210 220 230 240 250
    DSKILIEQAV SGCEVGCAVL GNSAALVVGE VDQIRLQYGI FRIHQEVEPE
    260 270 280 290 300
    KGSENAVITV PADLSAEERG RIQETAKKIY KALGCRGLAR VDMFLQDNGR
    310 320 330 340
    IVLNEVNTLP GFTSYSRYPR MMAAAGIALP ELIDRLIVLA LKG
    Length:343
    Mass (Da):37,443
    Last modified:May 1, 1992 - v1
    Checksum:iAAA48E3B2AD48E03
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X56895 Genomic DNA. Translation: CAA40215.1.
    M97297 Genomic DNA. Translation: AAA65956.1.
    PIRiS12254. CESOVM.
    RefSeqiWP_001079845.1. NZ_LXWZ01000240.1.
    YP_001019035.1. NC_008821.1.
    YP_001974796.1. NC_010980.1.
    YP_002128399.1. NC_011140.1.
    YP_976077.1. NC_008768.1.

    Genome annotation databases

    GeneIDi4670249.
    4783144.
    6385877.
    6779647.
    KEGGiag:CAA40215.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X56895 Genomic DNA. Translation: CAA40215.1.
    M97297 Genomic DNA. Translation: AAA65956.1.
    PIRiS12254. CESOVM.
    RefSeqiWP_001079845.1. NZ_LXWZ01000240.1.
    YP_001019035.1. NC_008821.1.
    YP_001974796.1. NC_010980.1.
    YP_002128399.1. NC_011140.1.
    YP_976077.1. NC_008768.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1E4EX-ray2.50A/B1-343[»]
    ProteinModelPortaliP25051.
    SMRiP25051.
    ModBaseiSearch...
    MobiDBiSearch...

    Chemistry databases

    BindingDBiP25051.
    ChEMBLiCHEMBL6037.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    GeneIDi4670249.
    4783144.
    6385877.
    6779647.
    KEGGiag:CAA40215.

    Phylogenomic databases

    KOiK15739.

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-15466.
    BRENDAi6.1.2.1. 2096.
    SABIO-RKP25051.

    Miscellaneous databases

    EvolutionaryTraceiP25051.
    PROiP25051.

    Family and domain databases

    Gene3Di3.30.1490.20. 1 hit.
    3.30.470.20. 2 hits.
    3.40.50.20. 1 hit.
    HAMAPiMF_00047. Dala_Dala_lig. 1 hit.
    InterProiIPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR000291. D-Ala_lig_Van_CS.
    IPR005905. D_ala_D_ala.
    IPR011095. Dala_Dala_lig_C.
    IPR011127. Dala_Dala_lig_N.
    IPR016185. PreATP-grasp_dom.
    [Graphical view]
    PfamiPF07478. Dala_Dala_lig_C. 1 hit.
    PF01820. Dala_Dala_lig_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF039102. Ddl/VanB. 1 hit.
    SUPFAMiSSF52440. SSF52440. 1 hit.
    TIGRFAMsiTIGR01205. D_ala_D_alaTIGR. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 1 hit.
    PS00843. DALA_DALA_LIGASE_1. 1 hit.
    PS00844. DALA_DALA_LIGASE_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiVANA_ENTFC
    AccessioniPrimary (citable) accession number: P25051
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: May 1, 1992
    Last modified: November 30, 2016
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing, Plasmid

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.