P25046 (MED19_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mediator of RNA polymerase II transcription subunit 19

Alternative name(s):
Hypoxic gene repressor protein 3
Mediator complex subunit 19
Negative regulator of URS2 protein 3
SNF1 suppressor protein 7

Gene names

Name:	ROX3
Synonyms:	MED19, NUT3, SSN7
Ordered Locus Names:	YBL093C
ORF Names:	YBL0837

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	220 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins. Ref.7 Ref.13 Ref.14 Ref.16
Subunit structure	Component of the Mediator complex, which is composed of at least 21 subunits that form three structurally distinct submodules. The Mediator head module contains MED6, MED8, MED11, SRB4/MED17, SRB5/MED18, ROX3/MED19, SRB2/MED20 and SRB6/MED22, the middle module contains MED1, MED4, NUT1/MED5, MED7, CSE2/MED9, NUT2/MED10, SRB7/MED21 and SOH1/MED31, and the tail module contains MED2, PGD1/MED3, RGR1/MED14, GAL11/MED15 and SIN4/MED16. The head and the middle modules interact directly with RNA polymerase II, whereas the elongated tail module interacts with gene-specific regulatory proteins. Ref.7 Ref.16
Subcellular location	Nucleus Ref.8 Ref.15.
Miscellaneous	Present with 6116 molecules/cell in log phase SD medium. Ref.9
Sequence similarities	Belongs to the Mediator complex subunit 19 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Molecular function	Activator
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	RNA polymerase II transcriptional preinitiation complex assembly Inferred from mutant phenotype. Source: SGD negative regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype. Source: SGD positive regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype. Source: SGD transcriptional open complex formation at RNA polymerase II promoter Inferred from mutant phenotype. Source: SGD
Cellular component	core mediator complex Inferred from direct assay. Source: SGD mediator complex Inferred from electronic annotation. Source: InterPro
Molecular function	RNA polymerase II transcription cofactor activity Inferred from electronic annotation. Source: InterPro RNA polymerase II transcription factor recruiting transcription factor activity Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 220	220	Mediator of RNA polymerase II transcription subunit 19		PRO_0000096370
Experimental info
	Sequence conflict	18	1	D → V in CAA56009. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P25046 [UniParc]. Last modified November 1, 1997. Version 3. Checksum: 1400C6554D233144	FASTA	220	24,857
	10 20 30 40 50 60 MASRVDETTV PSYYYYVDPE TTYTYQQPNP LQDLISVYGL DDISRQVART NLDGTKAVKL 70 80 90 100 110 120 RKSYKNQIAD LSGKFSTIPT RENGKGGQIA HILFQNNPDM MIQPPQQGQN MSEQQWREQL 130 140 150 160 170 180 RNRDIALFQP PNFDWDLCSS VLSQFERSYP SEFANQNQGG AQAPFDIDDL AFDLDGTGKS 190 200 210 220 QSGSNSGNNS KKRKNKSSGS SMATPTHSDS HEDMKRRRLE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The ROX3 gene encodes an essential nuclear protein involved in CYC7 gene expression in Saccharomyces cerevisiae." Rosenblum-Vos L.S., Rhodes L., Evangelista C.C. Jr., Boakye K.A., Zitomer R.S. Mol. Cell. Biol. 11:5639-5647(1991) [PubMed: 1656237] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces cerevisiae chromosome II." Obermaier B., Gassenhuber J., Piravandi E., Domdey H. Yeast 11:1103-1112(1995) [PubMed: 7502586] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Identification of Rox3 as a component of mediator and RNA polymerase II holoenzyme." Gustafsson C.M., Myers L.C., Li Y., Redd M.J., Lui M., Erdjument-Bromage H., Tempst P., Kornberg R.D. J. Biol. Chem. 272:48-50(1997) [PubMed: 8995225] [Abstract] Cited for: PROTEIN SEQUENCE OF 148-172, COMPONENT OF MEDIATOR COMPLEX.
[7]	"The structural and functional organization of the yeast mediator complex." Kang J.S., Kim S.H., Hwang M.S., Han S.J., Lee Y.C., Kim Y.-J. J. Biol. Chem. 276:42003-42010(2001) [PubMed: 11555651] [Abstract] Cited for: INTERACTION WITH SRB4, FUNCTION OF THE MEDIATOR COMPLEX, INTERACTION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
[8]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]	"A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II." Bourbon H.-M., Aguilera A., Ansari A.Z., Asturias F.J., Berk A.J., Bjoerklund S., Blackwell T.K., Borggrefe T., Carey M., Carlson M., Conaway J.W., Conaway R.C., Emmons S.W., Fondell J.D., Freedman L.P., Fukasawa T., Gustafsson C.M., Han M. , He X., Herman P.K., Hinnebusch A.G., Holmberg S., Holstege F.C.P., Jaehning J.A., Kim Y.-J., Kuras L., Leutz A., Lis J.T., Meisterernest M., Naeaer A.M., Nasmyth K., Parvin J.D., Ptashne M., Reinberg D., Ronne H., Sadowski I., Sakurai H., Sipiczki M., Sternberg P.W., Stillman D.J., Strich R., Struhl K., Svejstrup J.Q., Tuck S., Winston F., Roeder R.G., Kornberg R.D. Mol. Cell 14:553-557(2004) [PubMed: 15175151] [Abstract] Cited for: NOMENCLATURE.
[11]	"A high resolution protein interaction map of the yeast Mediator complex." Guglielmi B., van Berkum N.L., Klapholz B., Bijma T., Boube M., Boschiero C., Bourbon H.-M., Holstege F.C.P., Werner M. Nucleic Acids Res. 32:5379-5391(2004) [PubMed: 15477388] [Abstract] Cited for: TOPOLOGY OF THE MEDIATOR COMPLEX.
[12]	"Preponderance of free mediator in the yeast Saccharomyces cerevisiae." Takagi Y., Chadick J.Z., Davis J.A., Asturias F.J. J. Biol. Chem. 280:31200-31207(2005) [PubMed: 16002404] [Abstract] Cited for: CHARACTERIZATION OF THE MEDIATOR COMPLEX.
[13]	"Mediator and TFIIH govern carboxyl-terminal domain-dependent transcription in yeast extracts." Nair D., Kim Y., Myers L.C. J. Biol. Chem. 280:33739-33748(2005) [PubMed: 16076843] [Abstract] Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
[14]	"Mediator as a general transcription factor." Takagi Y., Kornberg R.D. J. Biol. Chem. 281:80-89(2006) [PubMed: 16263706] [Abstract] Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
[15]	"Genome-wide location of the coactivator mediator: binding without activation and transient Cdk8 interaction on DNA." Andrau J.-C., van de Pasch L., Lijnzaad P., Bijma T., Koerkamp M.G., van de Peppel J., Werner M., Holstege F.C.P. Mol. Cell 22:179-192(2006) [PubMed: 16630888] [Abstract] Cited for: SUBCELLULAR LOCATION.
[16]	"Med19(Rox3) regulates intermodule interactions in the Saccharomyces cerevisiae mediator complex." Baidoobonso S.M., Guidi B.W., Myers L.C. J. Biol. Chem. 282:5551-5559(2007) [PubMed: 17192271] [Abstract] Cited for: FUNCTION, CHARACTERIZATION OF THE MEDIATOR COMPLEX, INTERACTION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
[17]	"Structure of the yeast RNA polymerase II holoenzyme: mediator conformation and polymerase interaction." Davis J.A., Takagi Y., Kornberg R.D., Asturias F.J. Mol. Cell 10:409-415(2002) [PubMed: 12191485] [Abstract] Cited for: ELECTRON MICROSCOPY OF MEDIATOR COMPLEX IN COMPLEX WITH RNA POLYMERASE II.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X58300 Genomic DNA. Translation: CAA41233.1. X79489 Genomic DNA. Translation: CAA56009.1. Z35854 Genomic DNA. Translation: CAA84915.1. AY558292 Genomic DNA. Translation: AAS56618.1. BK006936 Genomic DNA. Translation: DAA07030.1.
PIR	S45409.
RefSeq	NP_009459.1. NM_001178333.1.
3D structure databases
ProteinModelPortal	P25046.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2336N.
IntAct	P25046. 42 interactions.
MINT	MINT-601707.
STRING	P25046.
Proteomic databases
PeptideAtlas	P25046.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBL093C; YBL093C; YBL093C.
GeneID	852184.
KEGG	sce:YBL093C.
NMPDR	fig|4932.3.peg.145.
Organism-specific databases
CYGD	YBL093c.
SGD	S000000189. ROX3.
Phylogenomic databases
eggNOG	fuNOG09306.
HOGENOM	HBG204148.
OMA	RENGKGG.
OrthoDB	EOG43247K.
Gene expression databases
ArrayExpress	P25046.
Genevestigator	P25046.
GermOnline	YBL093C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013942. Mediator_Med19_fun. [Graphical view]
KO	K15138.
Pfam	PF08633. Rox3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	970649.

Entry information

Entry name

MED19_YEAST

Accession

Primary (citable) accession number: P25046
Secondary accession number(s): D6VPR0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	November 1, 1997
Last modified:	December 14, 2011
This is version 100 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program