Reviewed, UniProtKB/Swiss-Prot P25044 (PTP1_YEAST)

Names and origin

Protein names

Recommended name:
    Tyrosine-protein phosphatase 1
    EC=3.1.3.48
Alternative name(s):
    Protein-tyrosine phosphatase 1
      Short name=PTPase 1

Gene names

Name:	PTP1
Ordered Locus Names:	YDL230W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Is not required for vegetative growth.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Subcellular location	Cytoplasm.
Miscellaneous	Present with 2690 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid dephosphorylation Inferred from direct assay. Source: SGD
Cellular component	mitochondrion Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct protein tyrosine phosphatase activity Ref.1 Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P43582	1	EBI-14183,EBI-22766
PRP40	P33203	1	EBI-14183,EBI-701
RSP5	P39940	1	EBI-14183,EBI-16219
SSM4	P40318	1	EBI-14183,EBI-18208
URN1	Q06525	1	EBI-14183,EBI-35138

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	Tyrosine-protein phosphatase 1		PRO_0000094855
Regions
	Domain	15 – 328	314	Tyrosine-protein phosphatase
Sites
	Active site	252	1	Phosphocysteine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P25044-1 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 15F71E50694BE562	FASTA	335	38,868
	10 20 30 40 50 60 MAAAPWYIRQ RDTDLLGKFK FIQNQEDGRL REATNGTVNS RWSLGVSIEP RNDARNRYVN 70 80 90 100 110 120 IMPYERNRVH LKTLSGNDYI NASYVKVNVP GQSIEPGYYI ATQGPTRKTW DQFWQMCYHN 130 140 150 160 170 180 CPLDNIVIVM VTPLVEYNRE KCYQYWPRGG VDDTVRIASK WESPGGANDM TQFPSDLKIE 190 200 210 220 230 240 FVNVHKVKDY YTVTDIKLTP TDPLVGPVKT VHHFYFDLWK DMNKPEEVVP IMELCAHSHS 250 260 270 280 290 300 LNSRGNPIIV HCSAGVGRTG TFIALDHLMH DTLDFKNITE RSRHSDRATE EYTRDLIEQI 310 320 330 VLQLRSQRMK MVQTKDQFLF IYHAAKYLNS LSVNQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of a yeast protein tyrosine phosphatase." Guan K., Deschenes R.J., Qiu H., Dixon J.E. J. Biol. Chem. 266:12964-12970(1991) [PubMed: 1649172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M64062 Genomic DNA. Translation: AAA34923.1. Z74278 Genomic DNA. Translation: CAA98809.1.
PIR	A39862.
RefSeq	NP_010051.1.
3D structure databases
HSSP	HSSP built from PDB template 1LAR based on UniProtKB P10586.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:2765N.
IntAct	P25044. 10 interactions.
Proteomic databases
PeptideAtlas	P25044.
Genome annotation databases
Ensembl	YDL230W. Saccharomyces cerevisiae. [Contig view]
GeneID	851368.
GenomeReviews	Gene locus YDL230W in contig Z71256_GR.
KEGG	sce:YDL230W.
NMPDR	fig|4932.3.peg.784.
Organism-specific databases
CYGD	YDL230w.
SGD	S000002389. PTP1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P25044.
OMA	P25044. NDARNRY.
Enzyme and pathway databases
BRENDA	3.1.3.48. 250.
Gene expression databases
ArrayExpress	P25044.
GermOnline	YDL230W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016277. Non-rcpt_Tyr_Pase_T1_fun. IPR000387. Tyr_Pase. IPR016130. Tyr_Pase_AS. IPR000242. Tyr_Pase_rcpt/non-rcpt. [Graphical view]
Pfam	PF00102. Y_phosphatase. 1 hit. [Graphical view]
PIRSF	PIRSF000938. PTPN1_yeast. 1 hit.
PRINTS	PR00700. PRTYPHPHTASE.
SMART	SM00194. PTPc. 1 hit. [Graphical view]
PROSITE	PS00383. TYR_PHOSPHATASE_1. 1 hit. PS50056. TYR_PHOSPHATASE_2. 1 hit. PS50055. TYR_PHOSPHATASE_PTP. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	968486.

Entry information

Entry name

PTP1_YEAST

Accession

Primary (citable) accession number: P25044

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	June 16, 2009
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)