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Protein

Elongation factor G, mitochondrial

Gene

MEF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.UniRule annotation

Miscellaneous

Present with 6348 molecules/cell in log phase SD medium.1 Publication

Pathwayi: polypeptide chain elongation

This protein is involved in the pathway polypeptide chain elongation, which is part of Protein biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway polypeptide chain elongation and in Protein biosynthesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi77 – 84GTPUniRule annotation8
Nucleotide bindingi148 – 152GTPUniRule annotation5
Nucleotide bindingi202 – 205GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

  • mitochondrial translation Source: SGD
  • mitochondrial translational elongation Source: SGD

Keywordsi

Molecular functionElongation factor
Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32222-MONOMER
UniPathwayiUPA00345

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor G, mitochondrialUniRule annotation
Short name:
EF-GmtUniRule annotation
Alternative name(s):
Elongation factor G 1, mitochondrialUniRule annotation
Short name:
mEF-G 1UniRule annotation
Elongation factor G1UniRule annotation
Gene namesi
Name:MEF1UniRule annotation
Ordered Locus Names:YLR069C
ORF Names:L2195
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR069C
SGDiS000004059 MEF1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 42MitochondrionUniRule annotationAdd BLAST42
ChainiPRO_000000744943 – 761Elongation factor G, mitochondrialAdd BLAST719

Post-translational modificationi

The precursor is processed in two steps involving mitochondrial intermediate peptidase (MIP) and mitochondrial processing peptidase (MPP).UniRule annotation

Proteomic databases

MaxQBiP25039
PaxDbiP25039
PRIDEiP25039

PTM databases

iPTMnetiP25039

Interactioni

Protein-protein interaction databases

BioGridi31343, 67 interactors
IntActiP25039, 3 interactors
MINTiP25039
STRINGi4932.YLR069C

Structurei

3D structure databases

ProteinModelPortaliP25039
SMRiP25039
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini68 – 349tr-type GAdd BLAST282

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00550000074911
HOGENOMiHOG000231585
InParanoidiP25039
KOiK02355
OMAiISRIYQM
OrthoDBiEOG092C1RRE

Family and domain databases

CDDicd16262 EFG_III, 1 hit
cd01434 EFG_mtEFG1_IV, 1 hit
cd04097 mtEFG1_C, 1 hit
Gene3Di3.30.230.10, 2 hits
HAMAPiMF_00054_B EF_G_EF_2_B, 1 hit
InterProiView protein in InterPro
IPR009022 EFG_III
IPR035647 EFG_III/V
IPR035649 EFG_V
IPR000640 EFG_V-like
IPR004161 EFTu-like_2
IPR031157 G_TR_CS
IPR027417 P-loop_NTPase
IPR020568 Ribosomal_S5_D2-typ_fold
IPR014721 Ribosomal_S5_D2-typ_fold_subgr
IPR005225 Small_GTP-bd_dom
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
IPR004540 Transl_elong_EFG/EF2
IPR005517 Transl_elong_EFG/EF2_IV
PfamiView protein in Pfam
PF00679 EFG_C, 1 hit
PF03764 EFG_IV, 1 hit
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PRINTSiPR00315 ELONGATNFCT
SMARTiView protein in SMART
SM00838 EFG_C, 1 hit
SM00889 EFG_IV, 1 hit
SUPFAMiSSF50447 SSF50447, 1 hit
SSF52540 SSF52540, 1 hit
SSF54211 SSF54211, 1 hit
SSF54980 SSF54980, 2 hits
TIGRFAMsiTIGR00484 EF-G, 1 hit
TIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25039-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVQKMMWVP RKMVGGRIPF FTCSKVFSGF SRRSFHESPL ARSTYEEEKV
60 70 80 90 100
LVDEIKQKLT PDDIGRCNKL RNIGISAHID SGKTTFTERV LYYTKRIKAI
110 120 130 140 150
HEVRGRDNVG AKMDSMDLER EKGITIQSAA TYCSWDKEGK NYHFNLIDTP
160 170 180 190 200
GHIDFTIEVE RALRVLDGAV LVVCAVSGVQ SQTVTVDRQM RRYNVPRVTF
210 220 230 240 250
INKMDRMGSD PFRAIEQLNS KLKIPAAAVQ IPIGSESSLS GVVDLINRVA
260 270 280 290 300
IYNKGDNGEI IEKGPVPENL KPLMEEKRQL LIETLADVDD EMAEMFLEEK
310 320 330 340 350
EPTTQQIKDA IRRSTIARSF TPVLMGSALA NTGIQPVLDA IVDYLPNPSE
360 370 380 390 400
VLNTALDVSN NEAKVNLVPA VQQPFVGLAF KLEEGKYGQL TYVRVYQGRL
410 420 430 440 450
RKGNYITNVK TGKKVKVARL VRMHSSEMED VDEVGSGEIC ATFGIDCASG
460 470 480 490 500
DTFTDGSVQY SMSSMYVPDA VVSLSITPNS KDASNFSKAL NRFQKEDPTF
510 520 530 540 550
RVKFDPESKE TIISGMGELH LEIYVERMRR EYNVDCVTGK PQVSYRESIT
560 570 580 590 600
IPADFDYTHK KQSGGAGQYG RVIGTLSPVD DITKGNIFET AIVGGRIPDK
610 620 630 640 650
YLAACGKGFE EVCEKGPLIG HRVLDVKMLI NDGAIHAVDS NELSFKTATM
660 670 680 690 700
SAFRDAFLRA QPVIMEPIMN VSVTSPNEFQ GNVIGLLNKL QAVIQDTENG
710 720 730 740 750
HDEFTLKAEC ALSTMFGFAT SLRASTQGKG EFSLEFSHYA PTAPHVQKEL
760
ISEFQKKQAK K
Length:761
Mass (Da):84,574
Last modified:July 15, 1998 - v2
Checksum:i3E2C534509B09103
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2S → C in AAT92963 (PubMed:17322287).Curated1
Sequence conflicti66R → I in CAA41267 (PubMed:1935960).Curated1
Sequence conflicti233I → V in CAA41267 (PubMed:1935960).Curated1
Sequence conflicti478P → S in CAA41267 (PubMed:1935960).Curated1
Sequence conflicti629L → P in CAA41267 (PubMed:1935960).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58378 Genomic DNA Translation: CAA41267.1
X94607 Genomic DNA Translation: CAA64315.1
Z73241 Genomic DNA Translation: CAA97626.1
AY692944 Genomic DNA Translation: AAT92963.1
BK006945 Genomic DNA Translation: DAA09386.1
PIRiS61642
RefSeqiNP_013170.1, NM_001181956.1

Genome annotation databases

EnsemblFungiiYLR069C; YLR069C; YLR069C
GeneIDi850758
KEGGisce:YLR069C

Similar proteinsi

Entry informationi

Entry nameiEFGM_YEAST
AccessioniPrimary (citable) accession number: P25039
Secondary accession number(s): D6VY70, Q6B1Y6, Q99360
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: July 15, 1998
Last modified: May 23, 2018
This is version 163 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

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