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Protein

Elongation factor G, mitochondrial

Gene

MEF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Mitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.UniRule annotation

Pathwayi: polypeptide chain elongation

This protein is involved in the pathway polypeptide chain elongation, which is part of Protein biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway polypeptide chain elongation and in Protein biosynthesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi77 – 84GTPUniRule annotation8
Nucleotide bindingi148 – 152GTPUniRule annotation5
Nucleotide bindingi202 – 205GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

  • mitochondrial translation Source: SGD
  • mitochondrial translational elongation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32222-MONOMER.
UniPathwayiUPA00345.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor G, mitochondrialUniRule annotation
Short name:
EF-GmtUniRule annotation
Alternative name(s):
Elongation factor G 1, mitochondrialUniRule annotation
Short name:
mEF-G 1UniRule annotation
Elongation factor G1UniRule annotation
Gene namesi
Name:MEF1UniRule annotation
Ordered Locus Names:YLR069C
ORF Names:L2195
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR069C.
SGDiS000004059. MEF1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 42MitochondrionUniRule annotationAdd BLAST42
ChainiPRO_000000744943 – 761Elongation factor G, mitochondrialAdd BLAST719

Post-translational modificationi

The precursor is processed in two steps involving mitochondrial intermediate peptidase (MIP) and mitochondrial processing peptidase (MPP).UniRule annotation

Proteomic databases

MaxQBiP25039.
PRIDEiP25039.

PTM databases

iPTMnetiP25039.

Interactioni

Protein-protein interaction databases

BioGridi31343. 49 interactors.
IntActiP25039. 3 interactors.
MINTiMINT-2788695.

Structurei

3D structure databases

ProteinModelPortaliP25039.
SMRiP25039.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini68 – 349tr-type GAdd BLAST282

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00550000074911.
HOGENOMiHOG000231585.
InParanoidiP25039.
KOiK02355.
OMAiKLGVAIQ.
OrthoDBiEOG092C1RRE.

Family and domain databases

CDDicd01434. EFG_mtEFG1_IV. 1 hit.
Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00054_B. EF_G_EF_2_B. 1 hit.
InterProiIPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004540. Transl_elong_EFG/EF2.
IPR005517. Transl_elong_EFG/EF2_IV.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00484. EF-G. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25039-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVQKMMWVP RKMVGGRIPF FTCSKVFSGF SRRSFHESPL ARSTYEEEKV
60 70 80 90 100
LVDEIKQKLT PDDIGRCNKL RNIGISAHID SGKTTFTERV LYYTKRIKAI
110 120 130 140 150
HEVRGRDNVG AKMDSMDLER EKGITIQSAA TYCSWDKEGK NYHFNLIDTP
160 170 180 190 200
GHIDFTIEVE RALRVLDGAV LVVCAVSGVQ SQTVTVDRQM RRYNVPRVTF
210 220 230 240 250
INKMDRMGSD PFRAIEQLNS KLKIPAAAVQ IPIGSESSLS GVVDLINRVA
260 270 280 290 300
IYNKGDNGEI IEKGPVPENL KPLMEEKRQL LIETLADVDD EMAEMFLEEK
310 320 330 340 350
EPTTQQIKDA IRRSTIARSF TPVLMGSALA NTGIQPVLDA IVDYLPNPSE
360 370 380 390 400
VLNTALDVSN NEAKVNLVPA VQQPFVGLAF KLEEGKYGQL TYVRVYQGRL
410 420 430 440 450
RKGNYITNVK TGKKVKVARL VRMHSSEMED VDEVGSGEIC ATFGIDCASG
460 470 480 490 500
DTFTDGSVQY SMSSMYVPDA VVSLSITPNS KDASNFSKAL NRFQKEDPTF
510 520 530 540 550
RVKFDPESKE TIISGMGELH LEIYVERMRR EYNVDCVTGK PQVSYRESIT
560 570 580 590 600
IPADFDYTHK KQSGGAGQYG RVIGTLSPVD DITKGNIFET AIVGGRIPDK
610 620 630 640 650
YLAACGKGFE EVCEKGPLIG HRVLDVKMLI NDGAIHAVDS NELSFKTATM
660 670 680 690 700
SAFRDAFLRA QPVIMEPIMN VSVTSPNEFQ GNVIGLLNKL QAVIQDTENG
710 720 730 740 750
HDEFTLKAEC ALSTMFGFAT SLRASTQGKG EFSLEFSHYA PTAPHVQKEL
760
ISEFQKKQAK K
Length:761
Mass (Da):84,574
Last modified:July 15, 1998 - v2
Checksum:i3E2C534509B09103
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2S → C in AAT92963 (PubMed:17322287).Curated1
Sequence conflicti66R → I in CAA41267 (PubMed:1935960).Curated1
Sequence conflicti233I → V in CAA41267 (PubMed:1935960).Curated1
Sequence conflicti478P → S in CAA41267 (PubMed:1935960).Curated1
Sequence conflicti629L → P in CAA41267 (PubMed:1935960).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58378 Genomic DNA. Translation: CAA41267.1.
X94607 Genomic DNA. Translation: CAA64315.1.
Z73241 Genomic DNA. Translation: CAA97626.1.
AY692944 Genomic DNA. Translation: AAT92963.1.
BK006945 Genomic DNA. Translation: DAA09386.1.
PIRiS61642.
RefSeqiNP_013170.1. NM_001181956.1.

Genome annotation databases

EnsemblFungiiYLR069C; YLR069C; YLR069C.
GeneIDi850758.
KEGGisce:YLR069C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58378 Genomic DNA. Translation: CAA41267.1.
X94607 Genomic DNA. Translation: CAA64315.1.
Z73241 Genomic DNA. Translation: CAA97626.1.
AY692944 Genomic DNA. Translation: AAT92963.1.
BK006945 Genomic DNA. Translation: DAA09386.1.
PIRiS61642.
RefSeqiNP_013170.1. NM_001181956.1.

3D structure databases

ProteinModelPortaliP25039.
SMRiP25039.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31343. 49 interactors.
IntActiP25039. 3 interactors.
MINTiMINT-2788695.

PTM databases

iPTMnetiP25039.

Proteomic databases

MaxQBiP25039.
PRIDEiP25039.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR069C; YLR069C; YLR069C.
GeneIDi850758.
KEGGisce:YLR069C.

Organism-specific databases

EuPathDBiFungiDB:YLR069C.
SGDiS000004059. MEF1.

Phylogenomic databases

GeneTreeiENSGT00550000074911.
HOGENOMiHOG000231585.
InParanoidiP25039.
KOiK02355.
OMAiKLGVAIQ.
OrthoDBiEOG092C1RRE.

Enzyme and pathway databases

UniPathwayiUPA00345.
BioCyciYEAST:G3O-32222-MONOMER.

Miscellaneous databases

PROiP25039.

Family and domain databases

CDDicd01434. EFG_mtEFG1_IV. 1 hit.
Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00054_B. EF_G_EF_2_B. 1 hit.
InterProiIPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004540. Transl_elong_EFG/EF2.
IPR005517. Transl_elong_EFG/EF2_IV.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00484. EF-G. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEFGM_YEAST
AccessioniPrimary (citable) accession number: P25039
Secondary accession number(s): D6VY70, Q6B1Y6, Q99360
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: July 15, 1998
Last modified: November 2, 2016
This is version 149 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6348 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.