Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ubiquitin carboxyl-terminal hydrolase 1

Gene

UBP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety in natural or engineered linear fusion proteins, irrespective of their size or the presence of an N-terminal extension to ubiquitin.

Miscellaneous

Present with 8970 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei110NucleophilePROSITE-ProRule annotation1
Active sitei697Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

  • thiol-dependent ubiquitin-specific protease activity Source: SGD

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-29521-MONOMER

Protein family/group databases

MEROPSiC19.002

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 1 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 1
Ubiquitin thioesterase 1
Ubiquitin-specific-processing protease 1
Gene namesi
Name:UBP1
Ordered Locus Names:YDL122W
ORF Names:D2250
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL122W
SGDiS000002280 UBP1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000805851 – 809Ubiquitin carboxyl-terminal hydrolase 1Add BLAST809

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei530PhosphoserineCombined sources1
Modified residuei531PhosphoserineCombined sources1
Modified residuei555PhosphoserineCombined sources1
Modified residuei618PhosphoserineCombined sources1
Modified residuei638PhosphoserineCombined sources1
Modified residuei652PhosphothreonineCombined sources1
Modified residuei653PhosphoserineCombined sources1
Modified residuei654PhosphoserineCombined sources1
Modified residuei670PhosphoserineCombined sources1
Modified residuei755PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP25037
PaxDbiP25037
PRIDEiP25037

PTM databases

iPTMnetiP25037

Interactioni

Protein-protein interaction databases

BioGridi31941, 196 interactors
IntActiP25037, 23 interactors
MINTiP25037
STRINGi4932.YDL122W

Structurei

3D structure databases

ProteinModelPortaliP25037
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini101 – 738USPAdd BLAST638

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated

Phylogenomic databases

HOGENOMiHOG000093934
InParanoidiP25037
KOiK11870
OMAiDPRTYMI
OrthoDBiEOG092C3TMU

Family and domain databases

InterProiView protein in InterPro
IPR001394 Peptidase_C19_UCH
IPR018200 USP_CS
IPR028889 USP_dom
PfamiView protein in Pfam
PF00443 UCH, 1 hit
PROSITEiView protein in PROSITE
PS00972 USP_1, 1 hit
PS00973 USP_2, 1 hit
PS50235 USP_3, 1 hit

Sequencei

Sequence statusi: Complete.

P25037-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLFIESKIN SLLQFLFGSR QDFLRNFKTW SNNNNNLSIY LLIFGIVVFF
60 70 80 90 100
YKKPDHLNYI VESVSEMTTN FRNNNSLSRW LPRSKFTHLD EEILKRGGFI
110 120 130 140 150
AGLVNDGNTC FMNSVLQSLA SSRELMEFLD NNVIRTYEEI EQNEHNEEGN
160 170 180 190 200
GQESAQDEAT HKKNTRKGGK VYGKHKKKLN RKSSSKEDEE KSQEPDITFS
210 220 230 240 250
VALRDLLSAL NAKYYRDKPY FKTNSLLKAM SKSPRKNILL GYDQEDAQEF
260 270 280 290 300
FQNILAELES NVKSLNTEKL DTTPVAKSEL PDDALVGQLN LGEVGTVYIP
310 320 330 340 350
TEQIDPNSIL HDKSIQNFTP FKLMTPLDGI TAERIGCLQC GENGGIRYSV
360 370 380 390 400
FSGLSLNLPN ENIGSTLKLS QLLSDWSKPE IIEGVECNRC ALTAAHSHLF
410 420 430 440 450
GQLKEFEKKP EGSIPEKLIN AVKDRVHQIE EVLAKPVIDD EDYKKLHTAN
460 470 480 490 500
MVRKCSKSKQ ILISRPPPLL SIHINRSVFD PRTYMIRKNN SKVLFKSRLN
510 520 530 540 550
LAPWCCDINE INLDARLPMS KKEKAAQQDS SEDENIGGEY YTKLHERFEQ
560 570 580 590 600
EFEDSEEEKE YDDAEGNYAS HYNHTKDISN YDPLNGEVDG VTSDDEDEYI
610 620 630 640 650
EETDALGNTI KKRIIEHSDV ENENVKDNEE LQEIDNVSLD EPKINVEDQL
660 670 680 690 700
ETSSDEEDVI PAPPINYARS FSTVPATPLT YSLRSVIVHY GTHNYGHYIA
710 720 730 740 750
FRKYRGCWWR ISDETVYVVD EAEVLSTPGV FMLFYEYDFD EETGKMKDDL
760 770 780 790 800
EAIQSNNEED DEKEQEQKGV QEPKESQEQG EGEEQEEGQE QMKFERTEDH

RDISGKDVN
Length:809
Mass (Da):92,769
Last modified:October 5, 2010 - v2
Checksum:i07BA266FB43C2C04
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti418L → P in AAA35189 (PubMed:2050695).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63484 Genomic DNA Translation: AAA35189.1
Z74170 Genomic DNA Translation: CAA98690.1
BK006938 Genomic DNA Translation: DAA11738.1
PIRiS67665
RefSeqiNP_010161.1, NM_001180181.1

Genome annotation databases

EnsemblFungiiYDL122W; YDL122W; YDL122W
GeneIDi851435
KEGGisce:YDL122W

Similar proteinsi

Entry informationi

Entry nameiUBP1_YEAST
AccessioniPrimary (citable) accession number: P25037
Secondary accession number(s): D6VRM8, Q07543
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: October 5, 2010
Last modified: May 23, 2018
This is version 156 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health