P25028 (FSYA_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitosis initiation protein fs(1)Ya

Gene names

Name:	fs(1)Ya
ORF Names:	CG2707

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	696 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cell cycle-dependent nuclear envelope component required for embryonic mitosis.
Subcellular location	Nucleus envelope. Nucleus › nucleoplasm. Cytoplasm. Note: In the nuclear envelope during interphase to metaphase. And in the nucleoplasm and cytoplasm during anaphase and telophase.
Developmental stage	Maternal protein synthesized during postoogenic maturation and persisting throughout embryogenesis.
Miscellaneous	The OPA repeat-containing and the Ser/Thr-rich regions might be involved in protein-protein interactions with the major protein-rich layer of the nuclear envelope.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Cytoplasm Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from genetic interaction. Source: FlyBase cell division Inferred from electronic annotation. Source: UniProtKB-KW chromatin assembly or disassembly Inferred from mutant phenotype. Source: FlyBase chromosome condensation Inferred from mutant phenotype. Source: FlyBase mitosis Inferred from mutant phenotype Ref.1. Source: FlyBase pronuclear fusion Inferred from mutant phenotype. Source: FlyBase
Cellular component	cytoplasm Inferred from direct assay Ref.1. Source: FlyBase nuclear envelope Inferred from direct assay Ref.1. Source: FlyBase nucleoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: FlyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 696	696	Mitosis initiation protein fs(1)Ya		PRO_0000087358
Regions
	Region	448 – 696	249	Rich in charged AA
	Motif	512 – 520	9	Nuclear localization signal Probable
	Motif	534 – 538	5	Nuclear localization signal Probable
	Compositional bias	270 – 281	12	Gln-rich (OPA repeat)
Amino acid modifications
	Modified residue	478	1	Phosphothreonine Ref.7
	Modified residue	484	1	Phosphothreonine Ref.7
	Modified residue	489	1	Phosphothreonine Ref.7
Experimental info
	Sequence conflict	270	1	Missing in CAA16819. Ref.5
	Sequence conflict	412	1	K → E in CAA16819. Ref.5
	Sequence conflict	673	1	L → P in AAA65184. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P25028 [UniParc]. Last modified January 27, 2003. Version 4. Checksum: C938FFFB3D9A5481	FASTA	696	77,726
	10 20 30 40 50 60 MSFSNVLIMR QPDEGKCHIC KRVFCCGKCR QKHQFKAHAI AVREPLGLRS AGGGIIEHRH 70 80 90 100 110 120 QMESGATTIY VFCPICERRP LLLREEMHGE LLAHIETCHL PLRCRKCQRN YTRVDDLREF 130 140 150 160 170 180 SKCVDQQQSC TDVTGATETS KATLKKAANS TAISTQTSPS VTPISLINMR WKAKSRVTHE 190 200 210 220 230 240 EFISDSVSSI RNLSSFSNSS IRRSIGQLGV NPSETMEKGK VIRSTSTPLH VESVFAKPKE 250 260 270 280 290 300 PITFNASTGG HVSSIYHEEP SPTPESNPVQ QQQQQQQPLQ QRAWKMGARN KMSAATPLRQ 310 320 330 340 350 360 VMSKSIQKAF VEHGGMMVHQ PPSAVVQRRV RLDLSEHSSH EAAGSSALDL RLSPAMRRTQ 370 380 390 400 410 420 SESSASEVNS GSSSSYSTSR NADLCKRQFL LSAQKLTTES IIITRTNSSS QKTSSTVYNS 430 440 450 460 470 480 CESVEIIRST SESAEVCHVP AITPIRVTGA GINKKQIKFE TPPKSSQQMR SNGEGDETKD 490 500 510 520 530 540 QFFTPEPGTP EIPERRHRQA IVPRQLSGEF SPKKDKPKEK GLAVMALISP PLQQPRVRPP 550 560 570 580 590 600 LRECRQQRVY SGVQDVGEPE VVDAEEEDEV FRPTNASTCN DKKLEAPNSG RLWSLMSSMM 610 620 630 640 650 660 RLPASLRGER EKDRDRDRDS DKENAGSGSL IRRCASIAGS LVRPSARDSS MEDQQCLKRK 670 680 690 RTQTLDSQYC SPLSPSSSSK RYRIRPREPI ERMRRQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Drosophila maternal-effect gene fs(1)Ya encodes a cell cycle-dependent nuclear envelope component required for embryonic mitosis." Lin H., Wolfner M.F. Cell 64:49-62(1991) [PubMed: 1986869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Embryo.
[2]	Liu J., Wolfner M.F. Submitted (JAN-1993) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[5]	"From sequence to chromosome: the tip of the X chromosome of D. melanogaster." Benos P.V., Gatt M.K., Ashburner M., Murphy L., Harris D., Barrell B.G., Ferraz C., Vidal S., Brun C., Demailles J., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Borkova D., Minana B. , Kafatos F.C., Louis C., Siden-Kiamos I., Bolshakov S., Papagiannakis G., Spanos L., Cox S., Madueno E., de Pablos B., Modolell J., Peter A., Schoettler P., Werner M., Mourkioti F., Beinert N., Dowe G., Schaefer U., Jaeckle H., Bucheton A., Callister D.M., Campbell L.A., Darlamitsou A., Henderson N.S., McMillan P.J., Salles C., Tait E.A., Valenti P., Saunders R.D.C., Glover D.M. Science 287:2220-2222(2000) [PubMed: 10731137] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Oregon-R.
[6]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[7]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-478; THR-484 AND THR-489, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M38442 mRNA. Translation: AAA65184.1. AE014298 Genomic DNA. Translation: AAF45816.2. AL021728 Genomic DNA. Translation: CAA16819.1. AY069542 mRNA. Translation: AAL39687.1.
PIR	A38436. T13648.
RefSeq	NP_477493.2. NM_058145.3.
UniGene	Dm.2083.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-115N.
MINT	MINT-235388.
STRING	P25028.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0070485; FBpp0070463; FBgn0000927.
GeneID	31263.
KEGG	dme:Dmel_CG2707.
NMPDR	fig|7227.3.peg.16298.
Organism-specific databases
CTD	31263.
FlyBase	FBgn0000927. fs(1)Ya.
Phylogenomic databases
eggNOG	inNOG11478.
GeneTree	EMGT00050000007538.
InParanoid	P25028.
OMA	CASIAGS.
OrthoDB	EOG4CRJF9.
PhylomeDB	P25028.
Gene expression databases
Bgee	P25028.
GermOnline	CG2707. Drosophila melanogaster.
Family and domain databases
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 1 hit. Uncertain. [Graphical view]
ProtoNet	Search...
Other
NextBio	772737.

Entry information

Entry name

FSYA_DROME

Accession

Primary (citable) accession number: P25028
Secondary accession number(s): Q8T057, Q9W4W0, Q9W4W1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	January 27, 2003
Last modified:	January 25, 2012
This is version 102 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project