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P25023

- BKRB2_RAT

UniProt

P25023 - BKRB2_RAT

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Protein

B2 bradykinin receptor

Gene
Bdkrb2
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Receptor for bradykinin. It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.

GO - Molecular functioni

  1. beta-2 adrenergic receptor binding Source: RGD
  2. bradykinin receptor activity Source: RGD
  3. protein binding Source: RGD

GO - Biological processi

  1. acute inflammatory response to antigenic stimulus Source: RGD
  2. cellular response to hypoxia Source: RGD
  3. maintenance of blood-brain barrier Source: RGD
  4. negative regulation of blood pressure Source: RGD
  5. negative regulation of cell proliferation Source: RGD
  6. positive regulation of cytosolic calcium ion concentration Source: RGD
  7. response to drug Source: RGD
  8. sensory perception of pain Source: RGD
  9. smooth muscle contraction Source: InterPro
  10. vasoconstriction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
B2 bradykinin receptor
Short name:
B2R
Short name:
BK-2 receptor
Gene namesi
Name:Bdkrb2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 6

Organism-specific databases

RGDi2201. Bdkrb2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6565Extracellular Reviewed predictionAdd
BLAST
Transmembranei66 – 8924Helical; Name=1; Reviewed predictionAdd
BLAST
Topological domaini90 – 989Cytoplasmic Reviewed prediction
Transmembranei99 – 12325Helical; Name=2; Reviewed predictionAdd
BLAST
Topological domaini124 – 13613Extracellular Reviewed predictionAdd
BLAST
Transmembranei137 – 15822Helical; Name=3; Reviewed predictionAdd
BLAST
Topological domaini159 – 18022Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei181 – 20323Helical; Name=4; Reviewed predictionAdd
BLAST
Topological domaini204 – 22623Extracellular Reviewed predictionAdd
BLAST
Transmembranei227 – 25327Helical; Name=5; Reviewed predictionAdd
BLAST
Topological domaini254 – 27219Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei273 – 29725Helical; Name=6; Reviewed predictionAdd
BLAST
Topological domaini298 – 31619Extracellular Reviewed predictionAdd
BLAST
Transmembranei317 – 34024Helical; Name=7; Reviewed predictionAdd
BLAST
Topological domaini341 – 39656Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 396396B2 bradykinin receptorPRO_0000069194Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi33 – 331N-linked (GlcNAc...) Reviewed prediction
Glycosylationi44 – 441N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi135 ↔ 216 By similarity
Modified residuei161 – 1611Phosphotyrosine1 Publication
Glycosylationi212 – 2121N-linked (GlcNAc...) Reviewed prediction
Modified residuei352 – 3521Phosphotyrosine1 Publication
Lipidationi356 – 3561S-palmitoyl cysteine1 Publication
Modified residuei365 – 3651Phosphoserine1 Publication
Modified residuei371 – 3711Phosphoserine1 Publication
Modified residuei374 – 3741Phosphothreonine1 Publication
Modified residuei378 – 3781Phosphoserine; by GRK61 Publication
Modified residuei380 – 3801Phosphoserine; by GRK61 Publication

Post-translational modificationi

Diphosphorylation at Ser-365 and Ser-371, at Ser-378 and Ser-380, and at Thr-374 and Ser-380 seem to be correlated pairwise.
Palmitoylation at Cys-356 and phosphorylation at Tyr-352 seem to be mutually exclusive.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP25023.
PRIDEiP25023.

PTM databases

PhosphoSiteiP25023.

Expressioni

Tissue specificityi

Uterus, vas deferens, kidney, ileum, heart, testis, lung and brain.

Gene expression databases

GenevestigatoriP25023.

Interactioni

Subunit structurei

Forms a complex with PECAM1 and GNAQ. Interacts with PECAM1 By similarity.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000035441.

Structurei

3D structure databases

ProteinModelPortaliP25023.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG148810.
GeneTreeiENSGT00720000108671.
HOGENOMiHOG000010314.
HOVERGENiHBG103183.
InParanoidiP25023.
KOiK03916.
OrthoDBiEOG7HHWSV.
PhylomeDBiP25023.

Family and domain databases

Gene3Di1.20.1070.10. 1 hit.
InterProiIPR001504. Brdyknn_2_rcpt.
IPR000496. Brdyknn_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00425. BRADYKININR.
PR00994. BRADYKINNB2R.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Long (identifier: P25023-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MDTRSSLCPK TQAVVAVFWG PGCHLSTCIE MFNITTQALG SAHNGTFSEV    50
NCPDTEWWSW LNAIQAPFLW VLFLLAALEN IFVLSVFCLH KTNCTVAEIY 100
LGNLAAADLI LACGLPFWAI TIANNFDWLF GEVLCRVVNT MIYMNLYSSI 150
CFLMLVSIDR YLALVKTMSM GRMRGVRWAK LYSLVIWSCT LLLSSPMLVF 200
RTMKDYREEG HNVTACVIVY PSRSWEVFTN MLLNLVGFLL PLSIITFCTV 250
RIMQVLRNNE MKKFKEVQTE KKATVLVLAV LGLFVLCWFP FQISTFLDTL 300
LRLGVLSGCW NERAVDIVTQ ISSYVAYSNS CLNPLVYVIV GKRFRKKSRE 350
VYQAICRKGG CMGESVQMEN SMGTLRTSIS VDRQIHKLQD WAGNKQ 396
Length:396
Mass (Da):44,930
Last modified:December 15, 1998 - v4
Checksum:i535676FCA74E92B3
GO
Isoform Short (identifier: P25023-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-30: Missing.

Show »
Length:366
Mass (Da):41,710
Checksum:iAD2AAF8840869FF5
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 3030Missing in isoform Short. VSP_001867Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti106 – 1061A → G1 Publication
Sequence conflicti106 – 1061A → G1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M59967 mRNA. Translation: AAA16425.1.
M59967 mRNA. Translation: AAA16426.1.
X80187 Genomic DNA. No translation available.
X80188 Genomic DNA. No translation available.
X80189 Genomic DNA. No translation available.
X80190 Genomic DNA. No translation available.
L26173 Genomic DNA. Translation: AAA62492.1.
X69681 mRNA. Translation: CAA49361.1.
PIRiA41283. OORTB2.
RefSeqiNP_775123.2. NM_173100.2.
UniGeneiRn.9845.

Genome annotation databases

EnsembliENSRNOT00000071735; ENSRNOP00000064715; ENSRNOG00000047300. [P25023-2]
GeneIDi25245.
KEGGirno:25245.
UCSCiRGD:2201. rat. [P25023-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M59967 mRNA. Translation: AAA16425.1 .
M59967 mRNA. Translation: AAA16426.1 .
X80187 Genomic DNA. No translation available.
X80188 Genomic DNA. No translation available.
X80189 Genomic DNA. No translation available.
X80190 Genomic DNA. No translation available.
L26173 Genomic DNA. Translation: AAA62492.1 .
X69681 mRNA. Translation: CAA49361.1 .
PIRi A41283. OORTB2.
RefSeqi NP_775123.2. NM_173100.2.
UniGenei Rn.9845.

3D structure databases

ProteinModelPortali P25023.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000035441.

Chemistry

BindingDBi P25023.
ChEMBLi CHEMBL2501.
GuidetoPHARMACOLOGYi 42.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei P25023.

Proteomic databases

PaxDbi P25023.
PRIDEi P25023.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000071735 ; ENSRNOP00000064715 ; ENSRNOG00000047300 . [P25023-2 ]
GeneIDi 25245.
KEGGi rno:25245.
UCSCi RGD:2201. rat. [P25023-1 ]

Organism-specific databases

CTDi 624.
RGDi 2201. Bdkrb2.

Phylogenomic databases

eggNOGi NOG148810.
GeneTreei ENSGT00720000108671.
HOGENOMi HOG000010314.
HOVERGENi HBG103183.
InParanoidi P25023.
KOi K03916.
OrthoDBi EOG7HHWSV.
PhylomeDBi P25023.

Miscellaneous databases

NextBioi 605845.
PROi P25023.

Gene expression databases

Genevestigatori P25023.

Family and domain databases

Gene3Di 1.20.1070.10. 1 hit.
InterProi IPR001504. Brdyknn_2_rcpt.
IPR000496. Brdyknn_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view ]
Pfami PF00001. 7tm_1. 1 hit.
[Graphical view ]
PRINTSi PR00425. BRADYKININR.
PR00994. BRADYKINNB2R.
PR00237. GPCRRHODOPSN.
PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Uterus.
  2. "Molecular structure and expression of rat bradykinin B2 receptor gene. Evidence for alternative splicing."
    Pesquero J.B., Lindsey C.J., Zeh K., Paiva A.C.M., Ganten D., Bader M.
    J. Biol. Chem. 269:26920-26925(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM SHORT).
    Strain: Sprague-Dawley.
  3. Bader M.
    Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  4. "Molecular cloning and sequence analysis of rat bradykinin B2 receptor gene."
    Wang D.Z., Ma J.X., Chao L., Chao J.
    Biochim. Biophys. Acta 1219:171-174(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM SHORT).
    Strain: Sprague-Dawley.
  5. "Cloned murine bradykinin receptor exhibits a mixed B1 and B2 pharmacological selectivity."
    McIntyre P., Phillips E., Skidmore E., Brown M., Webb M.
    Mol. Pharmacol. 44:346-355(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
    Strain: Sprague-Dawley.
    Tissue: Lung.
  6. Cited for: PROTEIN SEQUENCE OF 1-18.
  7. "Correlations in palmitoylation and multiple phosphorylation of rat bradykinin B2 receptor in Chinese hamster ovary cells."
    Soskic V., Nyakatura E., Roos M., Mueller-Esterl W., Godovac-Zimmermann J.
    J. Biol. Chem. 274:8539-8545(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: PALMITOYLATION AT CYS-356, PHOSPHORYLATION AT TYR-161; TYR-352; SER-365; SER-371; THR-374; SER-378 AND SER-380, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiBKRB2_RAT
AccessioniPrimary (citable) accession number: P25023
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: December 15, 1998
Last modified: June 11, 2014
This is version 130 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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