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P25023 (BKRB2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
B2 bradykinin receptor

Short name=B2R
Short name=BK-2 receptor
Gene names
Name:Bdkrb2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length396 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for bradykinin. It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.

Subunit structure

Forms a complex with PECAM1 and GNAQ. Interacts with PECAM1 By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Uterus, vas deferens, kidney, ileum, heart, testis, lung and brain.

Post-translational modification

Diphosphorylation at Ser-365 and Ser-371, at Ser-378 and Ser-380, and at Thr-374 and Ser-380 seem to be correlated pairwise.

Palmitoylation at Cys-356 and phosphorylation at Tyr-352 seem to be mutually exclusive. Ref.7

Sequence similarities

Belongs to the G-protein coupled receptor 1 family. Bradykinin receptor subfamily. BDKRB2 sub-subfamily.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
Lipoprotein
Palmitate
Phosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processacute inflammatory response to antigenic stimulus

Inferred from mutant phenotype PubMed 7791078. Source: RGD

cellular response to hypoxia

Inferred from expression pattern PubMed 19565561. Source: RGD

maintenance of blood-brain barrier

Inferred from direct assay PubMed 20080302. Source: RGD

negative regulation of blood pressure

Inferred from mutant phenotype PubMed 16507603. Source: RGD

negative regulation of cell proliferation

Inferred from mutant phenotype PubMed 12177051. Source: RGD

positive regulation of cytosolic calcium ion concentration

Inferred from mutant phenotype PubMed 15576455. Source: RGD

response to drug

Inferred from expression pattern PubMed 18190998. Source: RGD

sensory perception of pain

Traceable author statement Ref.1. Source: RGD

smooth muscle contraction

Inferred from electronic annotation. Source: InterPro

vasoconstriction

Inferred from electronic annotation. Source: InterPro

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from direct assay PubMed 16754789. Source: RGD

   Molecular_functionbeta-2 adrenergic receptor binding

Inferred from physical interaction PubMed 20150590. Source: RGD

bradykinin receptor activity

Inferred from direct assay Ref.1. Source: RGD

protein binding

Inferred from physical interaction PubMed 12177051PubMed 15607753PubMed 16754789. Source: RGD

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: P25023-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: P25023-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-30: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 396396B2 bradykinin receptor
PRO_0000069194

Regions

Topological domain1 – 6565Extracellular Potential
Transmembrane66 – 8924Helical; Name=1; Potential
Topological domain90 – 989Cytoplasmic Potential
Transmembrane99 – 12325Helical; Name=2; Potential
Topological domain124 – 13613Extracellular Potential
Transmembrane137 – 15822Helical; Name=3; Potential
Topological domain159 – 18022Cytoplasmic Potential
Transmembrane181 – 20323Helical; Name=4; Potential
Topological domain204 – 22623Extracellular Potential
Transmembrane227 – 25327Helical; Name=5; Potential
Topological domain254 – 27219Cytoplasmic Potential
Transmembrane273 – 29725Helical; Name=6; Potential
Topological domain298 – 31619Extracellular Potential
Transmembrane317 – 34024Helical; Name=7; Potential
Topological domain341 – 39656Cytoplasmic Potential

Amino acid modifications

Modified residue1611Phosphotyrosine Ref.7
Modified residue3521Phosphotyrosine Ref.7
Modified residue3651Phosphoserine Ref.7
Modified residue3711Phosphoserine Ref.7
Modified residue3741Phosphothreonine Ref.7
Modified residue3781Phosphoserine; by GRK6 Ref.7
Modified residue3801Phosphoserine; by GRK6 Ref.7
Lipidation3561S-palmitoyl cysteine Ref.7
Glycosylation331N-linked (GlcNAc...) Potential
Glycosylation441N-linked (GlcNAc...) Potential
Glycosylation2121N-linked (GlcNAc...) Potential
Disulfide bond135 ↔ 216 By similarity

Natural variations

Alternative sequence1 – 3030Missing in isoform Short.
VSP_001867

Experimental info

Sequence conflict1061A → G Ref.2
Sequence conflict1061A → G Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified December 15, 1998. Version 4.
Checksum: 535676FCA74E92B3

FASTA39644,930
        10         20         30         40         50         60 
MDTRSSLCPK TQAVVAVFWG PGCHLSTCIE MFNITTQALG SAHNGTFSEV NCPDTEWWSW 

        70         80         90        100        110        120 
LNAIQAPFLW VLFLLAALEN IFVLSVFCLH KTNCTVAEIY LGNLAAADLI LACGLPFWAI 

       130        140        150        160        170        180 
TIANNFDWLF GEVLCRVVNT MIYMNLYSSI CFLMLVSIDR YLALVKTMSM GRMRGVRWAK 

       190        200        210        220        230        240 
LYSLVIWSCT LLLSSPMLVF RTMKDYREEG HNVTACVIVY PSRSWEVFTN MLLNLVGFLL 

       250        260        270        280        290        300 
PLSIITFCTV RIMQVLRNNE MKKFKEVQTE KKATVLVLAV LGLFVLCWFP FQISTFLDTL 

       310        320        330        340        350        360 
LRLGVLSGCW NERAVDIVTQ ISSYVAYSNS CLNPLVYVIV GKRFRKKSRE VYQAICRKGG 

       370        380        390 
CMGESVQMEN SMGTLRTSIS VDRQIHKLQD WAGNKQ 

« Hide

Isoform Short [UniParc].

Checksum: AD2AAF8840869FF5
Show »

FASTA36641,710

References

[1]"Expression cloning of a rat B2 bradykinin receptor."
McEachern A.E., Shelton E.R., Bhakta S., Obernolte R., Bach C., Zuppan P., Fujisaki J., Aldrich R.W., Jarnagin K.
Proc. Natl. Acad. Sci. U.S.A. 88:7724-7728(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Uterus.
[2]"Molecular structure and expression of rat bradykinin B2 receptor gene. Evidence for alternative splicing."
Pesquero J.B., Lindsey C.J., Zeh K., Paiva A.C.M., Ganten D., Bader M.
J. Biol. Chem. 269:26920-26925(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM SHORT).
Strain: Sprague-Dawley.
[3]Bader M.
Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[4]"Molecular cloning and sequence analysis of rat bradykinin B2 receptor gene."
Wang D.Z., Ma J.X., Chao L., Chao J.
Biochim. Biophys. Acta 1219:171-174(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM SHORT).
Strain: Sprague-Dawley.
[5]"Cloned murine bradykinin receptor exhibits a mixed B1 and B2 pharmacological selectivity."
McIntyre P., Phillips E., Skidmore E., Brown M., Webb M.
Mol. Pharmacol. 44:346-355(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
Strain: Sprague-Dawley.
Tissue: Lung.
[6]"Structure of the bradykinin B2 receptors' amino terminus."
Abdalla S., Godovac-Zimmermann J., Braun A., Roscher A.A., Mueller-Esterl W., Quitterer U.
Biochemistry 35:7514-7519(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-18.
[7]"Correlations in palmitoylation and multiple phosphorylation of rat bradykinin B2 receptor in Chinese hamster ovary cells."
Soskic V., Nyakatura E., Roos M., Mueller-Esterl W., Godovac-Zimmermann J.
J. Biol. Chem. 274:8539-8545(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: PALMITOYLATION AT CYS-356, PHOSPHORYLATION AT TYR-161; TYR-352; SER-365; SER-371; THR-374; SER-378 AND SER-380, IDENTIFICATION BY MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M59967 mRNA. Translation: AAA16425.1.
M59967 mRNA. Translation: AAA16426.1.
X80187 Genomic DNA. No translation available.
X80188 Genomic DNA. No translation available.
X80189 Genomic DNA. No translation available.
X80190 Genomic DNA. No translation available.
L26173 Genomic DNA. Translation: AAA62492.1.
X69681 mRNA. Translation: CAA49361.1.
PIROORTB2. A41283.
RefSeqNP_775123.2. NM_173100.2.
UniGeneRn.9845.

3D structure databases

ProteinModelPortalP25023.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000035441.

Chemistry

BindingDBP25023.
ChEMBLCHEMBL2501.
GuidetoPHARMACOLOGY42.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP25023.

Proteomic databases

PaxDbP25023.
PRIDEP25023.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000071735; ENSRNOP00000064715; ENSRNOG00000047300. [P25023-2]
GeneID25245.
KEGGrno:25245.
UCSCRGD:2201. rat. [P25023-1]

Organism-specific databases

CTD624.
RGD2201. Bdkrb2.

Phylogenomic databases

eggNOGNOG148810.
GeneTreeENSGT00720000108671.
HOGENOMHOG000010314.
HOVERGENHBG103183.
InParanoidP25023.
KOK03916.
OrthoDBEOG7HHWSV.
PhylomeDBP25023.

Gene expression databases

GenevestigatorP25023.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR001504. Brdyknn_2_rcpt.
IPR000496. Brdyknn_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00425. BRADYKININR.
PR00994. BRADYKINNB2R.
PR00237. GPCRRHODOPSN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio605845.
PROP25023.

Entry information

Entry nameBKRB2_RAT
AccessionPrimary (citable) accession number: P25023
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: December 15, 1998
Last modified: June 11, 2014
This is version 130 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries