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P25023

- BKRB2_RAT

UniProt

P25023 - BKRB2_RAT

Protein

B2 bradykinin receptor

Gene

Bdkrb2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 4 (15 Dec 1998)
      Previous versions | rss
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    Functioni

    Receptor for bradykinin. It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.

    GO - Molecular functioni

    1. beta-2 adrenergic receptor binding Source: RGD
    2. bradykinin receptor activity Source: RGD
    3. protein binding Source: RGD

    GO - Biological processi

    1. acute inflammatory response to antigenic stimulus Source: RGD
    2. cellular response to hypoxia Source: RGD
    3. maintenance of blood-brain barrier Source: RGD
    4. negative regulation of blood pressure Source: RGD
    5. negative regulation of cell proliferation Source: RGD
    6. positive regulation of cytosolic calcium ion concentration Source: RGD
    7. response to drug Source: RGD
    8. sensory perception of pain Source: RGD
    9. smooth muscle contraction Source: InterPro
    10. vasoconstriction Source: InterPro

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    B2 bradykinin receptor
    Short name:
    B2R
    Short name:
    BK-2 receptor
    Gene namesi
    Name:Bdkrb2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 6

    Organism-specific databases

    RGDi2201. Bdkrb2.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: RGD

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 396396B2 bradykinin receptorPRO_0000069194Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi33 – 331N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi44 – 441N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi135 ↔ 216PROSITE-ProRule annotation
    Modified residuei161 – 1611Phosphotyrosine1 Publication
    Glycosylationi212 – 2121N-linked (GlcNAc...)Sequence Analysis
    Modified residuei352 – 3521Phosphotyrosine1 Publication
    Lipidationi356 – 3561S-palmitoyl cysteine1 Publication
    Modified residuei365 – 3651Phosphoserine1 Publication
    Modified residuei371 – 3711Phosphoserine1 Publication
    Modified residuei374 – 3741Phosphothreonine1 Publication
    Modified residuei378 – 3781Phosphoserine; by GRK61 Publication
    Modified residuei380 – 3801Phosphoserine; by GRK61 Publication

    Post-translational modificationi

    Diphosphorylation at Ser-365 and Ser-371, at Ser-378 and Ser-380, and at Thr-374 and Ser-380 seem to be correlated pairwise.
    Palmitoylation at Cys-356 and phosphorylation at Tyr-352 seem to be mutually exclusive.1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

    Proteomic databases

    PaxDbiP25023.
    PRIDEiP25023.

    PTM databases

    PhosphoSiteiP25023.

    Expressioni

    Tissue specificityi

    Uterus, vas deferens, kidney, ileum, heart, testis, lung and brain.

    Gene expression databases

    GenevestigatoriP25023.

    Interactioni

    Subunit structurei

    Forms a complex with PECAM1 and GNAQ. Interacts with PECAM1 By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000035441.

    Structurei

    3D structure databases

    ProteinModelPortaliP25023.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 6565ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini90 – 989CytoplasmicSequence Analysis
    Topological domaini124 – 13613ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini159 – 18022CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini204 – 22623ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini254 – 27219CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini298 – 31619ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini341 – 39656CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei66 – 8924Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei99 – 12325Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei137 – 15822Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei181 – 20323Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei227 – 25327Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei273 – 29725Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei317 – 34024Helical; Name=7Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family. Bradykinin receptor subfamily. BDKRB2 sub-subfamily.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG148810.
    GeneTreeiENSGT00720000108671.
    HOGENOMiHOG000010314.
    HOVERGENiHBG103183.
    InParanoidiP25023.
    KOiK03916.
    OrthoDBiEOG7HHWSV.
    PhylomeDBiP25023.

    Family and domain databases

    Gene3Di1.20.1070.10. 1 hit.
    InterProiIPR001504. Brdyknn_2_rcpt.
    IPR000496. Brdyknn_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view]
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR00425. BRADYKININR.
    PR00994. BRADYKINNB2R.
    PR00237. GPCRRHODOPSN.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Long (identifier: P25023-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDTRSSLCPK TQAVVAVFWG PGCHLSTCIE MFNITTQALG SAHNGTFSEV    50
    NCPDTEWWSW LNAIQAPFLW VLFLLAALEN IFVLSVFCLH KTNCTVAEIY 100
    LGNLAAADLI LACGLPFWAI TIANNFDWLF GEVLCRVVNT MIYMNLYSSI 150
    CFLMLVSIDR YLALVKTMSM GRMRGVRWAK LYSLVIWSCT LLLSSPMLVF 200
    RTMKDYREEG HNVTACVIVY PSRSWEVFTN MLLNLVGFLL PLSIITFCTV 250
    RIMQVLRNNE MKKFKEVQTE KKATVLVLAV LGLFVLCWFP FQISTFLDTL 300
    LRLGVLSGCW NERAVDIVTQ ISSYVAYSNS CLNPLVYVIV GKRFRKKSRE 350
    VYQAICRKGG CMGESVQMEN SMGTLRTSIS VDRQIHKLQD WAGNKQ 396
    Length:396
    Mass (Da):44,930
    Last modified:December 15, 1998 - v4
    Checksum:i535676FCA74E92B3
    GO
    Isoform Short (identifier: P25023-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-30: Missing.

    Show »
    Length:366
    Mass (Da):41,710
    Checksum:iAD2AAF8840869FF5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti106 – 1061A → G(PubMed:7929432)Curated
    Sequence conflicti106 – 1061A → G(PubMed:8086459)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 3030Missing in isoform Short. 1 PublicationVSP_001867Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M59967 mRNA. Translation: AAA16425.1.
    M59967 mRNA. Translation: AAA16426.1.
    X80187 Genomic DNA. No translation available.
    X80188 Genomic DNA. No translation available.
    X80189 Genomic DNA. No translation available.
    X80190 Genomic DNA. No translation available.
    L26173 Genomic DNA. Translation: AAA62492.1.
    X69681 mRNA. Translation: CAA49361.1.
    PIRiA41283. OORTB2.
    RefSeqiNP_775123.2. NM_173100.2.
    UniGeneiRn.9845.

    Genome annotation databases

    EnsembliENSRNOT00000071735; ENSRNOP00000064715; ENSRNOG00000047300. [P25023-2]
    GeneIDi25245.
    KEGGirno:25245.
    UCSCiRGD:2201. rat. [P25023-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M59967 mRNA. Translation: AAA16425.1 .
    M59967 mRNA. Translation: AAA16426.1 .
    X80187 Genomic DNA. No translation available.
    X80188 Genomic DNA. No translation available.
    X80189 Genomic DNA. No translation available.
    X80190 Genomic DNA. No translation available.
    L26173 Genomic DNA. Translation: AAA62492.1 .
    X69681 mRNA. Translation: CAA49361.1 .
    PIRi A41283. OORTB2.
    RefSeqi NP_775123.2. NM_173100.2.
    UniGenei Rn.9845.

    3D structure databases

    ProteinModelPortali P25023.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000035441.

    Chemistry

    BindingDBi P25023.
    ChEMBLi CHEMBL2501.
    GuidetoPHARMACOLOGYi 42.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P25023.

    Proteomic databases

    PaxDbi P25023.
    PRIDEi P25023.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000071735 ; ENSRNOP00000064715 ; ENSRNOG00000047300 . [P25023-2 ]
    GeneIDi 25245.
    KEGGi rno:25245.
    UCSCi RGD:2201. rat. [P25023-1 ]

    Organism-specific databases

    CTDi 624.
    RGDi 2201. Bdkrb2.

    Phylogenomic databases

    eggNOGi NOG148810.
    GeneTreei ENSGT00720000108671.
    HOGENOMi HOG000010314.
    HOVERGENi HBG103183.
    InParanoidi P25023.
    KOi K03916.
    OrthoDBi EOG7HHWSV.
    PhylomeDBi P25023.

    Miscellaneous databases

    NextBioi 605845.
    PROi P25023.

    Gene expression databases

    Genevestigatori P25023.

    Family and domain databases

    Gene3Di 1.20.1070.10. 1 hit.
    InterProi IPR001504. Brdyknn_2_rcpt.
    IPR000496. Brdyknn_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view ]
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00425. BRADYKININR.
    PR00994. BRADYKINNB2R.
    PR00237. GPCRRHODOPSN.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Uterus.
    2. "Molecular structure and expression of rat bradykinin B2 receptor gene. Evidence for alternative splicing."
      Pesquero J.B., Lindsey C.J., Zeh K., Paiva A.C.M., Ganten D., Bader M.
      J. Biol. Chem. 269:26920-26925(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM SHORT).
      Strain: Sprague-Dawley.
    3. Bader M.
      Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
    4. "Molecular cloning and sequence analysis of rat bradykinin B2 receptor gene."
      Wang D.Z., Ma J.X., Chao L., Chao J.
      Biochim. Biophys. Acta 1219:171-174(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM SHORT).
      Strain: Sprague-Dawley.
    5. "Cloned murine bradykinin receptor exhibits a mixed B1 and B2 pharmacological selectivity."
      McIntyre P., Phillips E., Skidmore E., Brown M., Webb M.
      Mol. Pharmacol. 44:346-355(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
      Strain: Sprague-Dawley.
      Tissue: Lung.
    6. Cited for: PROTEIN SEQUENCE OF 1-18.
    7. "Correlations in palmitoylation and multiple phosphorylation of rat bradykinin B2 receptor in Chinese hamster ovary cells."
      Soskic V., Nyakatura E., Roos M., Mueller-Esterl W., Godovac-Zimmermann J.
      J. Biol. Chem. 274:8539-8545(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: PALMITOYLATION AT CYS-356, PHOSPHORYLATION AT TYR-161; TYR-352; SER-365; SER-371; THR-374; SER-378 AND SER-380, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiBKRB2_RAT
    AccessioniPrimary (citable) accession number: P25023
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: December 15, 1998
    Last modified: October 1, 2014
    This is version 131 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3