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Protein

B2 bradykinin receptor

Gene

Bdkrb2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for bradykinin. It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.

GO - Molecular functioni

  • beta-2 adrenergic receptor binding Source: RGD
  • bradykinin receptor activity Source: RGD

GO - Biological processi

  • acute inflammatory response to antigenic stimulus Source: RGD
  • cellular response to hypoxia Source: RGD
  • maintenance of blood-brain barrier Source: RGD
  • negative regulation of blood pressure Source: RGD
  • negative regulation of cell proliferation Source: RGD
  • positive regulation of cytosolic calcium ion concentration Source: RGD
  • response to drug Source: RGD
  • sensory perception of pain Source: RGD
  • smooth muscle contraction Source: InterPro
  • vasoconstriction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
B2 bradykinin receptor
Short name:
B2R
Short name:
BK-2 receptor
Gene namesi
Name:Bdkrb2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2201. Bdkrb2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 65ExtracellularSequence analysisAdd BLAST65
Transmembranei66 – 89Helical; Name=1Sequence analysisAdd BLAST24
Topological domaini90 – 98CytoplasmicSequence analysis9
Transmembranei99 – 123Helical; Name=2Sequence analysisAdd BLAST25
Topological domaini124 – 136ExtracellularSequence analysisAdd BLAST13
Transmembranei137 – 158Helical; Name=3Sequence analysisAdd BLAST22
Topological domaini159 – 180CytoplasmicSequence analysisAdd BLAST22
Transmembranei181 – 203Helical; Name=4Sequence analysisAdd BLAST23
Topological domaini204 – 226ExtracellularSequence analysisAdd BLAST23
Transmembranei227 – 253Helical; Name=5Sequence analysisAdd BLAST27
Topological domaini254 – 272CytoplasmicSequence analysisAdd BLAST19
Transmembranei273 – 297Helical; Name=6Sequence analysisAdd BLAST25
Topological domaini298 – 316ExtracellularSequence analysisAdd BLAST19
Transmembranei317 – 340Helical; Name=7Sequence analysisAdd BLAST24
Topological domaini341 – 396CytoplasmicSequence analysisAdd BLAST56

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2501.
GuidetoPHARMACOLOGYi42.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000691941 – 396B2 bradykinin receptorAdd BLAST396

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi33N-linked (GlcNAc...)Sequence analysis1
Glycosylationi44N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi135 ↔ 216PROSITE-ProRule annotation
Modified residuei161Phosphotyrosine1 Publication1
Glycosylationi212N-linked (GlcNAc...)Sequence analysis1
Modified residuei352Phosphotyrosine1 Publication1
Lipidationi356S-palmitoyl cysteine1 Publication1
Modified residuei365Phosphoserine1 Publication1
Modified residuei371Phosphoserine1 Publication1
Modified residuei374Phosphothreonine1 Publication1
Modified residuei378Phosphoserine; by GRK61 Publication1
Modified residuei380Phosphoserine; by GRK61 Publication1

Post-translational modificationi

Diphosphorylation at Ser-365 and Ser-371, at Ser-378 and Ser-380, and at Thr-374 and Ser-380 seem to be correlated pairwise.
Palmitoylation at Cys-356 and phosphorylation at Tyr-352 seem to be mutually exclusive.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP25023.
PRIDEiP25023.

PTM databases

iPTMnetiP25023.
PhosphoSitePlusiP25023.
SwissPalmiP25023.

Expressioni

Tissue specificityi

Uterus, vas deferens, kidney, ileum, heart, testis, lung and brain.

Gene expression databases

BgeeiENSRNOG00000047300.
GenevisibleiP25023. RN.

Interactioni

Subunit structurei

Forms a complex with PECAM1 and GNAQ. Interacts with PECAM1 (By similarity).By similarity

GO - Molecular functioni

  • beta-2 adrenergic receptor binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000064990.

Chemistry databases

BindingDBiP25023.

Structurei

3D structure databases

ProteinModelPortaliP25023.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Bradykinin receptor subfamily. BDKRB2 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410INT3. Eukaryota.
ENOG41117RA. LUCA.
HOGENOMiHOG000010314.
HOVERGENiHBG103183.
InParanoidiP25023.
KOiK03916.
PhylomeDBiP25023.

Family and domain databases

InterProiIPR001504. Brdyknn_2_rcpt.
IPR000496. Brdyknn_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00425. BRADYKININR.
PR00994. BRADYKINNB2R.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P25023-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDTRSSLCPK TQAVVAVFWG PGCHLSTCIE MFNITTQALG SAHNGTFSEV
60 70 80 90 100
NCPDTEWWSW LNAIQAPFLW VLFLLAALEN IFVLSVFCLH KTNCTVAEIY
110 120 130 140 150
LGNLAAADLI LACGLPFWAI TIANNFDWLF GEVLCRVVNT MIYMNLYSSI
160 170 180 190 200
CFLMLVSIDR YLALVKTMSM GRMRGVRWAK LYSLVIWSCT LLLSSPMLVF
210 220 230 240 250
RTMKDYREEG HNVTACVIVY PSRSWEVFTN MLLNLVGFLL PLSIITFCTV
260 270 280 290 300
RIMQVLRNNE MKKFKEVQTE KKATVLVLAV LGLFVLCWFP FQISTFLDTL
310 320 330 340 350
LRLGVLSGCW NERAVDIVTQ ISSYVAYSNS CLNPLVYVIV GKRFRKKSRE
360 370 380 390
VYQAICRKGG CMGESVQMEN SMGTLRTSIS VDRQIHKLQD WAGNKQ
Length:396
Mass (Da):44,930
Last modified:December 15, 1998 - v4
Checksum:i535676FCA74E92B3
GO
Isoform Short (identifier: P25023-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-30: Missing.

Show »
Length:366
Mass (Da):41,710
Checksum:iAD2AAF8840869FF5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti106A → G (PubMed:7929432).Curated1
Sequence conflicti106A → G (PubMed:8086459).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0018671 – 30Missing in isoform Short. 1 PublicationAdd BLAST30

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59967 mRNA. Translation: AAA16425.1.
M59967 mRNA. Translation: AAA16426.1.
X80187 Genomic DNA. No translation available.
X80188 Genomic DNA. No translation available.
X80189 Genomic DNA. No translation available.
X80190 Genomic DNA. No translation available.
L26173 Genomic DNA. Translation: AAA62492.1.
X69681 mRNA. Translation: CAA49361.1.
PIRiA41283. OORTB2.
RefSeqiNP_775123.2. NM_173100.2.
UniGeneiRn.9845.

Genome annotation databases

GeneIDi25245.
KEGGirno:25245.
UCSCiRGD:2201. rat. [P25023-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59967 mRNA. Translation: AAA16425.1.
M59967 mRNA. Translation: AAA16426.1.
X80187 Genomic DNA. No translation available.
X80188 Genomic DNA. No translation available.
X80189 Genomic DNA. No translation available.
X80190 Genomic DNA. No translation available.
L26173 Genomic DNA. Translation: AAA62492.1.
X69681 mRNA. Translation: CAA49361.1.
PIRiA41283. OORTB2.
RefSeqiNP_775123.2. NM_173100.2.
UniGeneiRn.9845.

3D structure databases

ProteinModelPortaliP25023.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000064990.

Chemistry databases

BindingDBiP25023.
ChEMBLiCHEMBL2501.
GuidetoPHARMACOLOGYi42.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP25023.
PhosphoSitePlusiP25023.
SwissPalmiP25023.

Proteomic databases

PaxDbiP25023.
PRIDEiP25023.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25245.
KEGGirno:25245.
UCSCiRGD:2201. rat. [P25023-1]

Organism-specific databases

CTDi624.
RGDi2201. Bdkrb2.

Phylogenomic databases

eggNOGiENOG410INT3. Eukaryota.
ENOG41117RA. LUCA.
HOGENOMiHOG000010314.
HOVERGENiHBG103183.
InParanoidiP25023.
KOiK03916.
PhylomeDBiP25023.

Miscellaneous databases

PROiP25023.

Gene expression databases

BgeeiENSRNOG00000047300.
GenevisibleiP25023. RN.

Family and domain databases

InterProiIPR001504. Brdyknn_2_rcpt.
IPR000496. Brdyknn_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00425. BRADYKININR.
PR00994. BRADYKINNB2R.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBKRB2_RAT
AccessioniPrimary (citable) accession number: P25023
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: December 15, 1998
Last modified: November 2, 2016
This is version 146 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.