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P25020

- SRC_RSVH1

UniProt

P25020 - SRC_RSVH1

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Protein

Tyrosine-protein kinase transforming protein Src

Gene

V-SRC

Organism
Rous sarcoma virus (strain H-19)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

This phosphoprotein, required for both the initiation and the maintenance of neoplastic transformation, is a protein kinase that catalyzes the phosphorylation of tyrosine residues in vitro.

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Cofactori

Manganese.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei295 – 2951ATPPROSITE-ProRule annotation
Active sitei386 – 3861Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi273 – 2819ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2. 5464.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase transforming protein Src (EC:2.7.10.2)
Alternative name(s):
pp60v-src
Short name:
p60-Src
Short name:
v-Src
Gene namesi
Name:V-SRC
OrganismiRous sarcoma virus (strain H-19)
Taxonomic identifieri11887 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeAlpharetrovirus
Virus hostiGallus gallus (Chicken) [TaxID: 9031]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed; by hostBy similarity
Chaini2 – 526525Tyrosine-protein kinase transforming protein SrcPRO_0000088155Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine; by host1 Publication
Modified residuei416 – 4161Phosphotyrosine; by autocatalysisBy similarity

Post-translational modificationi

The phosphorylated form is termed pp60v-src.

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
PDGFRBP096194EBI-8636140,EBI-641237From a different organism.

Protein-protein interaction databases

IntActiP25020. 1 interaction.
MINTiMINT-8013578.

Structurei

3D structure databases

ProteinModelPortaliP25020.
SMRiP25020. Positions 83-516.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini81 – 14262SH3PROSITE-ProRule annotationAdd
BLAST
Domaini148 – 24598SH2PROSITE-ProRule annotationAdd
BLAST
Domaini267 – 517251Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 SH2 domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH2 domain, SH3 domain

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
PR00109. TYRKINASE.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25020-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGSSKSKPKD PSQRRRSLEP PDSTHHGGFP ASQTPDETAA PDAHRNPSRS
60 70 80 90 100
FGTVATEPKL FWGFNTSDTV TSPQRAGALA GGVTTFVALY DYESWTETDL
110 120 130 140 150
SFKKGERLQI VNNTEGDWWL AHSLTTGQTG YIPSNYVAPS DSIQAEEWYF
160 170 180 190 200
GKITRRESER LLLNPENPRG TFLVRKSETA KGAYCLSVSD FDNAKGPNVK
210 220 230 240 250
HYKICKLYSG GFYITSRTQF GSLQQLVAYY SKHADGLCHR LTNVCPTSKP
260 270 280 290 300
QTQGLAKDAW EIPRESLRLE AKLGQGCFGE VWMGTWNGTT RVAIKTLKPG
310 320 330 340 350
TMSPEAFLQE AQVMKKLRHE KLVQLYAVVS EEPIYIVIEY MSKGSLLDFL
360 370 380 390 400
KGEMGKYLRL PQLVDMAAQI ASGMAYVERM NYVHRDLRAA NILVGENLVC
410 420 430 440 450
KVADFGLARL IEDNEYTARQ GAKFPIKWTA PEAALYGRFT IKSDVWSFGI
460 470 480 490 500
LLTELTTKGR VPYPGMVNRE VLDQVERAYR MPCPPECPES LHDLMCQCWR
510 520
KDPEERPTFK YLQAQLLPAC VLEVAE
Length:526
Mass (Da):59,075
Last modified:January 23, 2007 - v3
Checksum:i33B212BCC943C6AF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X15345 mRNA. Translation: CAA33404.1.
PIRiS09609. OKFVYR.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X15345 mRNA. Translation: CAA33404.1 .
PIRi S09609. OKFVYR.

3D structure databases

ProteinModelPortali P25020.
SMRi P25020. Positions 83-516.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P25020. 1 interaction.
MINTi MINT-8013578.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BRENDAi 2.7.10.2. 5464.

Family and domain databases

Gene3Di 3.30.505.10. 1 hit.
InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view ]
Pfami PF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view ]
PRINTSi PR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
PR00109. TYRKINASE.
SMARTi SM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view ]
SUPFAMi SSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete nucleotide sequence of LTR, v-src, LTR provirus H-19."
    Bodor J., Poliak E., Pichrtova J., Geryk J., Svoboda J.
    Nucleic Acids Res. 17:8869-8869(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Antibodies to an NH2-terminal myristoyl glycine moiety can detect NH2-terminal myristoylated proteins in the retrovirus-infected cells."
    Shoji S., Tashiro A., Furuishi K., Takenaka O., Kida Y., Horiuchi S., Funakoshi T., Kubota Y.
    Biochem. Biophys. Res. Commun. 162:724-732(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-9, MYRISTOYLATION AT GLY-2.
    Strain: Isolate tsNY68.

Entry informationi

Entry nameiSRC_RSVH1
AccessioniPrimary (citable) accession number: P25020
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 105 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3