Reviewed, UniProtKB/Swiss-Prot P25017 (TR2M2_AGRVI)

Names and origin

Protein names

Recommended name:
    Tryptophan 2-monooxygenase
    EC=1.13.12.3

Gene names

Name:

iaaM

Encoded on

Plasmid pTiTM4

Organism

Agrobacterium vitis (Rhizobium vitis)

Taxonomic identifier

373 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	755 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-tryptophan + O2 = (indol-3-yl)acetamide + CO2 + H2O.
Pathway	Plant hormone metabolism; auxin biosynthesis.
Sequence similarities	Belongs to the tryptophan 2-monooxygenase family.
Caution	The plasmid pTiTM4 carries two T-regions, the TA and TB region, both of which have a functional iaaM gene, with low homology between them.

Ontologies

Keywords
Biological process	Auxin biosynthesis
Disease	Crown gall tumor
Molecular function	Monooxygenase Oxidoreductase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	auxin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro tryptophan 2-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 755	755	Tryptophan 2-monooxygenase		PRO_0000065591

Sequences

	Sequence		Length	Mass (Da)
	P25017-1 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: 6FA63E502343136F	FASTA	755	83,973
	10 20 30 40 50 60 MSASSLLDKQ CDHFSTKIVD LIMVDKADEL DRRVAAAFSE REASRERRIS QISGECNAGL 70 80 90 100 110 120 ACKRLADGRF PEISAGQRVA VLSAYIYVGE EILRWILEPE ASVRTRVSGL VAIDLAPSCM 130 140 150 160 170 180 DISRAQLLQT MNLLSGKRCA PSDLSHFVAI SISETARSRT LQMAPYEEGS LKSVTGFTVI 190 200 210 220 230 240 IEEAVPFDMV AYGRNLMLKA SAGSFPTIDL LYDYRLFLDK CSDSGRIGFF PEDVPRPKVA 250 260 270 280 290 300 VIGAGISGLV VASELLHAGV DDVTIYEAGD RVGGKLWSHA FKDAPGVVAE MGAMRFPPAA 310 320 330 340 350 360 SCLFFFLERY GLSSMRPFPN PGTVDTDLVY EGCRYMWKAG QQPPKLFHRV YSGWHAFLKD 370 380 390 400 410 420 GFLEGDIVLA SPDAITEALK SGDIRRAHDS WQIWLNRFGR ESFSSAIERI FLGTHPPGGE 430 440 450 460 470 480 TWSFPHDWDL FKLMGIGSGG FGPVFESGFT EILRLVINGY EENQRMCSEG ISELPRRIAS 490 500 510 520 530 540 QVVNGVSVSQ RIRHVQVRAI EKEKTKIKIR LKSGISELYD KVVVTSGLAN IQLRHCLTCD 550 560 570 580 590 600 TTIFRAPVNQ AVDNSHMTGS SKLFLLTERK FWFDHMLPSC VLMDGFAKAV YCLDYEPQDP 610 620 630 640 650 660 NGKGLVLISY TWEDDSHKLL AVPDKKERLC LLRDAISKSF PVFAQHLVPA CADYDQNVVQ 670 680 690 700 710 720 HDWLTDENAG GRFKLNRRGE DFYSEELFFQ ALDTTNDTGV YLAGCSCSFT GGWVEGAIQT 730 740 750 ACNAVCAIIH NCGGILAKDN PLKHPWKRYN YRNRN

References

[1]	"Sequence of Agrobacterium tumefaciens biotype III auxin genes." Bonnard G., Vincent F., Otten L. Plant Mol. Biol. 16:733-738(1991) [PubMed: 1868204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: TM4.
[2]	Otten L., de Ruffray P. Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CG474.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X56185 Genomic DNA. Translation: CAA39646.1. U83987 Genomic DNA. Translation: AAB41874.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.13.12.3. 97084.
Family and domain databases
InterPro	IPR000759. Adrndx_reductase. IPR002937. Amino_oxidase. IPR006064. Glycosidase. IPR012142. Trp_2-mOase. [Graphical view]
Pfam	PF01593. Amino_oxidase. 1 hit. PF02027. RolB_RolC. 1 hit. [Graphical view]
PIRSF	PIRSF000319. Trp_2-mono_O2ase. 1 hit.
PRINTS	PR00419. ADXRDTASE.
ProtoNet	Search...

Entry information

Entry name

TR2M2_AGRVI

Accession

Primary (citable) accession number: P25017

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	June 16, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)