P25008 (CCNC_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cyclin-C

Gene names

Name:	CycC
Synonyms:	CLND
ORF Names:	CG7281

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Binds to and activates cyclin-dependent kinase Cdk8 that phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Required for leg and eye development and macrochaete specification or differentiation. Ref.1 Ref.2 Ref.6 Ref.7
Subunit structure	Component of the Cdk8 module of the Mediator complex, composed of CycC, Cdk8, kto and skd.
Subcellular location	Nucleus Ref.6.
Developmental stage	Expressed both maternally and zygotically during developmental periods of maximal cell division; most abundant in early embryos and low levels in larvae, pupae and adults. Ref.1
Sequence similarities	Belongs to the cyclin family. Cyclin C subfamily. Contains 1 cyclin N-terminal domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Molecular function	Cyclin Developmental protein Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	chaeta development Inferred from mutant phenotype Ref.7. Source: FlyBase imaginal disc-derived leg segmentation Inferred from mutant phenotype Ref.7. Source: FlyBase mitotic cell cycle G2/M transition DNA damage checkpoint Inferred from genetic interaction. Source: FlyBase regulation of cyclin-dependent protein kinase activity Inferred from electronic annotation. Source: InterPro sex comb development Inferred from mutant phenotype Ref.7. Source: FlyBase
Cellular component	DNA-directed RNA polymerase II, holoenzyme Inferred from electronic annotation. Source: InterPro mediator complex Inferred from direct assay Ref.7. Source: UniProtKB
Molecular function	RNA polymerase II transcription cofactor activity Inferred from mutant phenotype Ref.7. Source: UniProtKB cyclin-dependent protein kinase regulator activity Inferred from direct assay Ref.2. Source: FlyBase protein kinase binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
cdc2	P23572	4	EBI-195485,EBI-108689
Cdk5	P48609	4	EBI-195485,EBI-94216
Cdk8	Q9VT57	2	EBI-195485,EBI-163640
kto	Q9VW47	2	EBI-195485,EBI-139710
skd	Q7KTX8	2	EBI-195485,EBI-110730

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 267	267	Cyclin-C		PRO_0000080424
Regions
	Domain	48 – 151	104	Cyclin N-terminal

Sequences

	Sequence		Length	Mass (Da)
	P25008 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: DA73CB7EA89149FE	FASTA	267	31,292
	10 20 30 40 50 60 MAGNFWQSSH SQQWILDKPD LLRERQHDLL ALNEDEYQKV FIFFANVIQV LGEQLKLRQQ 70 80 90 100 110 120 VIATATVYFK RFYARNSLKN IDPLLLAPTC ILLASKVEEF GVISNSRLIS ICQSAIKTKF 130 140 150 160 170 180 SYAYAQEFPY RTNHILECEF YLLENLDCCL IVYQPYRPLL QLVQDMGQED QLLTLSWRIV 190 200 210 220 230 240 NDSLRTDVCL LYPPYQIAIA CLQIACVILQ KDATKQWFAE LNVDLDKVQE IVRAIVNLYE 250 260 LWKDWKEKDE IQMLLSKIPK PKPPPQR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel cyclin gene from Drosophila complements CLN function in yeast." Lahue E.E., Smith A.V., Orr-Weaver T.L. Genes Dev. 5:2166-2175(1991) [PubMed: 1836192] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE.
[2]	"An evolutionarily conserved cyclin homolog from Drosophila rescues yeast deficient in G1 cyclins." Leopold P., O'Farrell P.H. Cell 66:1207-1216(1991) [PubMed: 1833067] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[5]	Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[6]	"Drosophila Cdk8, a kinase partner of cyclin C that interacts with the large subunit of RNA polymerase II." Leclerc V., Tassan J.-P., O'Farrell P.H., Nigg E.A., Leopold P. Mol. Biol. Cell 7:505-513(1996) [PubMed: 8730095] [Abstract] Cited for: FUNCTION, INTERACTION, SUBCELLULAR LOCATION.
[7]	"Distinct roles for Mediator Cdk8 module subunits in Drosophila development." Loncle N., Boube M., Joulia L., Boschiero C., Werner M., Cribbs D.L., Bourbon H.-M. EMBO J. 26:1045-1054(2007) [PubMed: 17290221] [Abstract] Cited for: FUNCTION, INTERACTION WITH CYCC; KTO AND SKD.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X62948 mRNA. Translation: CAA44720.1. M74906 mRNA. No translation available. AE014297 Genomic DNA. Translation: AAF55109.1. AY061425 mRNA. Translation: AAL28973.1.
PIR	A40269.
RefSeq	NP_476848.1. NM_057500.3.
UniGene	Dm.20187.
3D structure databases
ProteinModelPortal	P25008.
SMR	P25008. Positions 1-258.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-17706N.
IntAct	P25008. 27 interactions.
MINT	MINT-280784.
STRING	P25008.
Proteomic databases
PRIDE	P25008.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0083010; FBpp0082469; FBgn0004597.
GeneID	41801.
KEGG	dme:Dmel_CG7281.
NMPDR	fig|7227.3.peg.12825.
Organism-specific databases
CTD	41801.
FlyBase	FBgn0004597. CycC.
Phylogenomic databases
eggNOG	inNOG06552.
GeneTree	EMGT00050000000363.
InParanoid	P25008.
OMA	FFANVIQ.
OrthoDB	EOG4GF1X0.
PhylomeDB	P25008.
Gene expression databases
Bgee	P25008.
GermOnline	CG7281. Drosophila melanogaster.
Family and domain databases
InterPro	IPR013763. Cyclin-like. IPR004367. Cyclin_C. IPR023598. Cyclin_C/H. IPR015429. Cyclin_C/H/T/L. IPR006671. Cyclin_N. [Graphical view]
Gene3D	G3DSA:1.10.472.10. Cyclin_related. 1 hit.
KO	K15161.
PANTHER	PTHR10026. Trans_reg_cyclin. 1 hit.
Pfam	PF02984. Cyclin_C. 1 hit. PF00134. Cyclin_N. 1 hit. [Graphical view]
PIRSF	PIRSF028758. PIRSF028758. 1 hit.
SMART	SM00385. CYCLIN. 2 hits. [Graphical view]
SUPFAM	SSF47954. Cyclin_like. 2 hits.
PROSITE	PS00292. CYCLINS. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	825634.

Entry information

Entry name

CCNC_DROME

Accession

Primary (citable) accession number: P25008
Secondary accession number(s): Q9VFF1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	January 25, 2012
This is version 110 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project