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Protein

Excitatory amino acid transporter 1

Gene

Slc1a3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:1279699, PubMed:8387171). Functions as a symporter that transports one amino acid molecule together with two or three Na+ ions and one proton, in parallel with the counter-transport of one K+ ion (By similarity). Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (By similarity).By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi394Sodium 1; via carbonyl oxygenBy similarity1
Metal bindingi396Sodium 2; via carbonyl oxygenBy similarity1
Metal bindingi398Sodium 1By similarity1
Binding sitei402AspartateBy similarity1
Binding sitei476AspartateBy similarity1
Metal bindingi483Sodium 1; via carbonyl oxygenBy similarity1
Binding sitei483AspartateBy similarity1
Metal bindingi487Sodium 1By similarity1

GO - Molecular functioni

  • glutamate:sodium symporter activity Source: UniProtKB
  • high-affinity glutamate transmembrane transporter activity Source: UniProtKB
  • L-glutamate transmembrane transporter activity Source: RGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • chloride transmembrane transport Source: UniProtKB
  • D-aspartate import across plasma membrane Source: UniProtKB
  • L-aspartate import across plasma membrane Source: UniProtKB
  • L-glutamate import across plasma membrane Source: UniProtKB
  • L-glutamate transport Source: RGD
  • malate-aspartate shuttle Source: RGD
  • neurotransmitter transport Source: RGD
  • potassium ion transmembrane transport Source: UniProtKB
  • response to inorganic substance Source: MGI

Keywordsi

Biological processAmino-acid transport, Symport, Transport
LigandChloride, Metal-binding, Potassium, Sodium

Protein family/group databases

TCDBi2.A.23.2.1. the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Excitatory amino acid transporter 1
Alternative name(s):
Glial glutamate transporter
Sodium-dependent glutamate/aspartate transporter 11 Publication
Short name:
GLAST1 Publication
Short name:
GLAST-11 Publication
Solute carrier family 1 member 3
Gene namesi
Name:Slc1a3
Synonyms:Eaat1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3698. Slc1a3.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 47CytoplasmicCuratedAdd BLAST47
Transmembranei48 – 68Helical; Name=1By similarityAdd BLAST21
Topological domaini69 – 86ExtracellularCuratedAdd BLAST18
Transmembranei87 – 108Helical; Name=2By similarityAdd BLAST22
Topological domaini109 – 122CytoplasmicCuratedAdd BLAST14
Transmembranei123 – 145Helical; Name=3By similarityAdd BLAST23
Topological domaini146 – 236ExtracellularCuratedAdd BLAST91
Transmembranei237 – 260Helical; Name=4By similarityAdd BLAST24
Topological domaini261 – 269CytoplasmicCurated9
Transmembranei270 – 297Helical; Name=5By similarityAdd BLAST28
Topological domaini298 – 318ExtracellularCuratedAdd BLAST21
Transmembranei319 – 340Helical; Name=6By similarityAdd BLAST22
Topological domaini341 – 345CytoplasmicCurated5
Intramembranei346 – 376Discontinuously helicalBy similarityAdd BLAST31
Topological domaini377 – 385CytoplasmicCurated9
Transmembranei386 – 412Helical; Name=7By similarityAdd BLAST27
Topological domaini413 – 425ExtracellularCuratedAdd BLAST13
Intramembranei426 – 459Discontinuously helicalBy similarityAdd BLAST34
Topological domaini460 – 472ExtracellularCuratedAdd BLAST13
Transmembranei473 – 494Helical; Name=8By similarityAdd BLAST22
Topological domaini495 – 543CytoplasmicCuratedAdd BLAST49

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4190.
GuidetoPHARMACOLOGYi868.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002020591 – 543Excitatory amino acid transporter 1Add BLAST543

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei512PhosphoserineCombined sources1

Post-translational modificationi

Glycosylated.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP24942.
PRIDEiP24942.

PTM databases

iPTMnetiP24942.
PhosphoSitePlusiP24942.
SwissPalmiP24942.

Expressioni

Tissue specificityi

Detected in brain and cerebellum (PubMed:1279699, PubMed:8387171). Both isoform GLAST-1 and GLAST-1A are expressed in bone and brain (PubMed:11086157). In brain isoform GLAST-1 is highly enriched in the Purkinje cell layer in cerebellum (PubMed:11086157).3 Publications

Interactioni

Subunit structurei

Homotrimer.By similarity

Protein-protein interaction databases

BioGridi248125. 1 interactor.
STRINGi10116.ENSRNOP00000022319.

Chemistry databases

BindingDBiP24942.

Structurei

3D structure databases

ProteinModelPortaliP24942.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni363 – 365Aspartate bindingBy similarity3
Regioni443 – 447Aspartate bindingBy similarity5

Domaini

Contains eight transmembrane regions plus two helical hairpins that dip into the membrane. These helical hairpin structures play an important role in the transport process. The first enters the membrane from the cytoplasmic side, the second one from the extracellular side. During the transport cycle, the regions involved in amino acid transport, and especially the helical hairpins, move vertically by about 15-18 Angstroms, alternating between exposure to the aqueous phase and reinsertion in the lipid bilayer. In contrast, the regions involved in trimerization do not move.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3787. Eukaryota.
COG1301. LUCA.
HOGENOMiHOG000208776.
HOVERGENiHBG000080.
InParanoidiP24942.
KOiK05614.
PhylomeDBiP24942.

Family and domain databases

Gene3Di1.10.3860.10. 1 hit.
InterProiView protein in InterPro
IPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
PfamiView protein in Pfam
PF00375. SDF. 1 hit.
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 2 hits.
PROSITEiView protein in PROSITE
PS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform GLAST-1 (identifier: P24942-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTKSNGEEPR MGSRMERFQQ GVRKRTLLAK KKVQNITKED VKSYLFRNAF
60 70 80 90 100
VLLTVSAVIV GTILGFALRP YKMSYREVKY FSFPGELLMR MLQMLVLPLI
110 120 130 140 150
ISSLVTGMAA LDSKASGKMG MRAVVYYMTT TIIAVVIGII IVIIIHPGKG
160 170 180 190 200
TKENMYREGK IVQVTAADAF LDLIRNMFPP NLVEACFKQF KTSYEKRSFK
210 220 230 240 250
VPIQANETLL GAVINNVSEA METLTRIREE MVPVPGSVNG VNALGLVVFS
260 270 280 290 300
MCFGFVIGNM KEQGQALREF FDSLNEAIMR LVAVIMWYAP LGILFLIAGK
310 320 330 340 350
ILEMEDMGVI GGQLAMYTVT VIVGLLIHAV IVLPLLYFLV TRKNPWVFIG
360 370 380 390 400
GLLQALITAL GTSSSSATLP ITFKCLEENN GVDKRITRFV LPVGATINMD
410 420 430 440 450
GTALYEALAA IFIAQVNNFD LNFGQIITIS ITATAASIGA AGIPQAGLVT
460 470 480 490 500
MVIVLTSVGL PTDDITLIIA VDWFLDRLRT TTNVLGDSLG AGIVEHLSRH
510 520 530 540
ELKNRDVEMG NSVIEENEMK KPYQLIAQDN EPEKPVADSE TKM
Length:543
Mass (Da):59,697
Last modified:April 1, 1993 - v2
Checksum:i5B01C220CA873BFD
GO
Isoform GLAST-1A (identifier: P24942-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     62-107: Missing.

Show »
Length:497
Mass (Da):54,391
Checksum:i125FFE15EB33F8CC
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti302L → V1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00626362 – 107Missing in isoform GLAST-1A. CuratedAdd BLAST46

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63744 mRNA. Translation: CAA45276.1.
S59158 mRNA. Translation: AAB26422.1.
AF265360 mRNA. Translation: AAF73069.1.
S75687 mRNA. Translation: AAB32664.1.
PIRiS26609.
RefSeqiNP_001276870.1. NM_001289941.1.
NP_001276871.1. NM_001289942.1.
NP_001276872.1. NM_001289943.1.
NP_062098.1. NM_019225.2.
UniGeneiRn.34134.

Genome annotation databases

GeneIDi29483.
KEGGirno:29483.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiEAA1_RAT
AccessioniPrimary (citable) accession number: P24942
Secondary accession number(s): Q9JK43
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: April 1, 1993
Last modified: August 30, 2017
This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families