Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Excitatory amino acid transporter 1

Gene

Slc1a3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.

GO - Molecular functioni

  • high-affinity glutamate transmembrane transporter activity Source: RGD
  • L-glutamate transmembrane transporter activity Source: RGD
  • sodium:dicarboxylate symporter activity Source: InterPro

GO - Biological processi

  • L-glutamate transmembrane transport Source: GOC
  • L-glutamate transport Source: RGD
  • malate-aspartate shuttle Source: RGD
  • neurotransmitter transport Source: RGD
  • response to inorganic substance Source: MGI
Complete GO annotation...

Keywords - Biological processi

Symport, Transport

Protein family/group databases

TCDBi2.A.23.2.1. the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Excitatory amino acid transporter 1
Alternative name(s):
Glial glutamate transporter
Sodium-dependent glutamate/aspartate transporter 1
Short name:
GLAST-1
Solute carrier family 1 member 3
Gene namesi
Name:Slc1a3
Synonyms:Eaat1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3698. Slc1a3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4747CytoplasmicSequence analysisAdd
BLAST
Transmembranei48 – 6821HelicalSequence analysisAdd
BLAST
Transmembranei91 – 11121HelicalSequence analysisAdd
BLAST
Transmembranei123 – 14523HelicalSequence analysisAdd
BLAST
Topological domaini146 – 23792ExtracellularSequence analysisAdd
BLAST
Transmembranei238 – 26023HelicalSequence analysisAdd
BLAST
Transmembranei281 – 30222HelicalSequence analysisAdd
BLAST
Transmembranei319 – 34022HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • basolateral plasma membrane Source: RGD
  • dendritic spine Source: RGD
  • integral component of membrane Source: UniProtKB-KW
  • mitochondrial inner membrane Source: RGD
  • neuron projection Source: RGD
  • plasma membrane Source: RGD
  • synapse Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL4190.
GuidetoPHARMACOLOGYi868.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 543543Excitatory amino acid transporter 1PRO_0000202059Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei512 – 5121PhosphoserineCombined sources

Post-translational modificationi

Glycosylated.

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP24942.
PRIDEiP24942.

PTM databases

iPTMnetiP24942.
PhosphoSiteiP24942.
SwissPalmiP24942.

Expressioni

Tissue specificityi

Both isoforms are expressed in bone and brain. In brain isoform GLAST-1 is highly enriched in the Purkinje cell layer in cerebellum.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi248125. 1 interaction.
STRINGi10116.ENSRNOP00000022319.

Chemistry

BindingDBiP24942.

Structurei

3D structure databases

ProteinModelPortaliP24942.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3787. Eukaryota.
COG1301. LUCA.
HOGENOMiHOG000208776.
HOVERGENiHBG000080.
InParanoidiP24942.
KOiK05614.
PhylomeDBiP24942.

Family and domain databases

Gene3Di1.10.3860.10. 2 hits.
InterProiIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 2 hits.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 2 hits.
PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform GLAST-1 (identifier: P24942-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTKSNGEEPR MGSRMERFQQ GVRKRTLLAK KKVQNITKED VKSYLFRNAF
60 70 80 90 100
VLLTVSAVIV GTILGFALRP YKMSYREVKY FSFPGELLMR MLQMLVLPLI
110 120 130 140 150
ISSLVTGMAA LDSKASGKMG MRAVVYYMTT TIIAVVIGII IVIIIHPGKG
160 170 180 190 200
TKENMYREGK IVQVTAADAF LDLIRNMFPP NLVEACFKQF KTSYEKRSFK
210 220 230 240 250
VPIQANETLL GAVINNVSEA METLTRIREE MVPVPGSVNG VNALGLVVFS
260 270 280 290 300
MCFGFVIGNM KEQGQALREF FDSLNEAIMR LVAVIMWYAP LGILFLIAGK
310 320 330 340 350
ILEMEDMGVI GGQLAMYTVT VIVGLLIHAV IVLPLLYFLV TRKNPWVFIG
360 370 380 390 400
GLLQALITAL GTSSSSATLP ITFKCLEENN GVDKRITRFV LPVGATINMD
410 420 430 440 450
GTALYEALAA IFIAQVNNFD LNFGQIITIS ITATAASIGA AGIPQAGLVT
460 470 480 490 500
MVIVLTSVGL PTDDITLIIA VDWFLDRLRT TTNVLGDSLG AGIVEHLSRH
510 520 530 540
ELKNRDVEMG NSVIEENEMK KPYQLIAQDN EPEKPVADSE TKM
Length:543
Mass (Da):59,697
Last modified:April 1, 1993 - v2
Checksum:i5B01C220CA873BFD
GO
Isoform GLAST-1A (identifier: P24942-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     62-107: Missing.

Show »
Length:497
Mass (Da):54,391
Checksum:i125FFE15EB33F8CC
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti302 – 3021L → V.1 Publication

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei62 – 10746Missing in isoform GLAST-1A. CuratedVSP_006263Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63744 mRNA. Translation: CAA45276.1.
S59158 mRNA. Translation: AAB26422.1.
AF265360 mRNA. Translation: AAF73069.1.
S75687 mRNA. Translation: AAB32664.1.
PIRiS26609.
RefSeqiNP_001276870.1. NM_001289941.1.
NP_001276871.1. NM_001289942.1.
NP_001276872.1. NM_001289943.1.
NP_062098.1. NM_019225.2.
UniGeneiRn.34134.

Genome annotation databases

GeneIDi29483.
KEGGirno:29483.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63744 mRNA. Translation: CAA45276.1.
S59158 mRNA. Translation: AAB26422.1.
AF265360 mRNA. Translation: AAF73069.1.
S75687 mRNA. Translation: AAB32664.1.
PIRiS26609.
RefSeqiNP_001276870.1. NM_001289941.1.
NP_001276871.1. NM_001289942.1.
NP_001276872.1. NM_001289943.1.
NP_062098.1. NM_019225.2.
UniGeneiRn.34134.

3D structure databases

ProteinModelPortaliP24942.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248125. 1 interaction.
STRINGi10116.ENSRNOP00000022319.

Chemistry

BindingDBiP24942.
ChEMBLiCHEMBL4190.
GuidetoPHARMACOLOGYi868.

Protein family/group databases

TCDBi2.A.23.2.1. the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family.

PTM databases

iPTMnetiP24942.
PhosphoSiteiP24942.
SwissPalmiP24942.

Proteomic databases

PaxDbiP24942.
PRIDEiP24942.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29483.
KEGGirno:29483.

Organism-specific databases

CTDi6507.
RGDi3698. Slc1a3.

Phylogenomic databases

eggNOGiKOG3787. Eukaryota.
COG1301. LUCA.
HOGENOMiHOG000208776.
HOVERGENiHBG000080.
InParanoidiP24942.
KOiK05614.
PhylomeDBiP24942.

Miscellaneous databases

PROiP24942.

Family and domain databases

Gene3Di1.10.3860.10. 2 hits.
InterProiIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 2 hits.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 2 hits.
PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure, expression, and functional analysis of a Na(+)-dependent glutamate/aspartate transporter from rat brain."
    Storck T., Schulte S., Hofmann K.O., Stoffel W.
    Proc. Natl. Acad. Sci. U.S.A. 89:10955-10959(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. Hofmann K.O.
    Submitted (JUN-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 16.
  3. "Expression cloning of a rat glutamate transporter."
    Tanaka K.
    Neurosci. Res. 16:149-153(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  4. "The open reading frame of the Na(+)-dependent glutamate transporter GLAST-1 is expressed in bone and a splice variant of this molecule is expressed in bone and brain."
    Huggett J., Vaughan-Thomas A., Mason D.
    FEBS Lett. 485:13-18(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, VARIANT VAL-302, ALTERNATIVE SPLICING.
    Strain: Wistar.
    Tissue: Bone and Cerebellum.
  5. "UDP galactose:ceramide galactosyltransferase and glutamate/aspartate transporter. Copurification, separation and characterization of the two glycoproteins."
    Schulte S., Stoffel W.
    Eur. J. Biochem. 233:947-953(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 18-23; 115-121; 159-170; 231-243 AND 506-534.
    Tissue: Brain.
  6. "Identification of a glutamate/aspartate transporter in the rat cochlea."
    Li H.S., Niedzielski A.S., Beisel K.W., Hiel H., Wenthold R.J., Morley B.J.
    Hear. Res. 78:235-242(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 172-501.
  7. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-512, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiEAA1_RAT
AccessioniPrimary (citable) accession number: P24942
Secondary accession number(s): Q9JK43
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: April 1, 1993
Last modified: June 8, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.