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Protein

Pre-hexon-linking protein VIII

Gene

L4

Organism
Human adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hexon-linking protein-N: Structural component of the virion that acts as a cement protein on the capsid interior and which glue the peripentonal hexons and group-of-nine hexons together.UniRule annotation1 Publication
Hexon-linking protein-C: Structural component of the virion that acts as a cement protein on the capsid interior and which glue the peripentonal hexons and group-of-nine hexons together.UniRule annotation1 Publication

Miscellaneous

All late proteins expressed from the major late promoter are produced by alternative splicing and alternative polyadenylation of the same gene giving rise to non-overlapping ORFs. A leader sequence is present in the N-terminus of all these mRNAs and is recognized by the viral shutoff protein to provide expression although conventional translation via ribosome scanning from the cap has been shut off in the host cell.UniRule annotation

GO - Molecular functioni

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-hexon-linking protein VIIIUniRule annotation
Alternative name(s):
Pre-protein VIIIUniRule annotation
Short name:
pVIIIUniRule annotation
Cleaved into the following 2 chains:
Hexon-linking protein-NUniRule annotation
Alternative name(s):
12.1 kDa protein VIIIUniRule annotation
Protein VIII-NUniRule annotation
Hexon-linking protein-CUniRule annotation
Alternative name(s):
7.6 kDa protein VIIIUniRule annotation
Protein VIII-CUniRule annotation
Gene namesi
Name:L4UniRule annotation
OrganismiHuman adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5)
Taxonomic identifieri28285 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAdenoviridaeMastadenovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000004992 Componenti: Genome

Subcellular locationi

Hexon-linking protein-C :
  • Virion UniRule annotation1 Publication

  • Note: Located on the inner side of the capsid shell. Present in 120 copies per virion.UniRule annotation1 Publication
Pre-hexon-linking protein VIII :
  • Host nucleus UniRule annotationCurated
Hexon-linking protein-N :
  • Virion UniRule annotation1 Publication

  • Note: Located on the inner side of the capsid shell. Present in 120 copies per virion.UniRule annotation1 Publication

GO - Cellular componenti

  • host cell nucleus Source: UniProtKB-SubCell
  • viral capsid Source: UniProtKB-KW
  • virion Source: UniProtKB

Keywords - Cellular componenti

Capsid protein, Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004211311 – 227Pre-hexon-linking protein VIIIUniRule annotationAdd BLAST227
PeptideiPRO_00000364951 – 111Hexon-linking protein-NUniRule annotationAdd BLAST111
PropeptideiPRO_0000421132112 – 157UniRule annotationAdd BLAST46
PeptideiPRO_0000036496158 – 227Hexon-linking protein-CUniRule annotationAdd BLAST70

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei64Phosphothreonine; by hostUniRule annotation1
Modified residuei118Phosphoserine; by hostUniRule annotation1
Modified residuei174Phosphoserine; by hostUniRule annotation1

Post-translational modificationi

Cleaved by the viral protease during virion maturation. May cause the middle segment to be shed from the capsid.UniRule annotation3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei111 – 112Cleavage; by viral proteaseUniRule annotation2
Sitei157 – 158Cleavage; by viral proteaseUniRule annotation2

Keywords - PTMi

Phosphoprotein

Expressioni

Inductioni

Expressed in the late phase of the viral replicative cycle.UniRule annotation

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Interacts with the peripentonal hexons as well as the hexons in the facets (PubMed:20798312, PubMed:25071205). Part of a complex composed of the core-capsid bridging protein, the endosome lysis protein VI and the hexon-linking protein VIII; these interactions bridge the virus core to the capsid (PubMed:25071205).UniRule annotation2 Publications

GO - Molecular functioni

Structurei

Secondary structure

1227
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi40 – 43Combined sources4
Helixi45 – 47Combined sources3
Helixi51 – 57Combined sources7
Beta strandi58 – 61Combined sources4
Beta strandi67 – 70Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3IYNelectron microscopy-O/P1-227[»]
4CWUX-ray3.80X/Y1-227[»]
SMRiP24936.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP24936.

Family & Domainsi

Sequence similaritiesi

Belongs to the adenoviridae hexon-linking protein family.UniRule annotationCurated

Phylogenomic databases

OrthoDBiVOG090000MV.

Family and domain databases

HAMAPiMF_04049. ADV_CAP8. 1 hit.
InterProiView protein in InterPro
IPR000646. Adeno_PVIII.
PfamiView protein in Pfam
PF01310. Adeno_PVIII. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24936-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKEIPTPYM WSYQPQMGLA AGAAQDYSTR INYMSAGPHM ISRVNGIRAH
60 70 80 90 100
RNRILLEQAA ITTTPRNNLN PRSWPAALVY QESPAPTTVV LPRDAQAEVQ
110 120 130 140 150
MTNSGAQLAG GFRHRVRSPG QGITHLTIRG RGIQLNDESV SSSLGLRPDG
160 170 180 190 200
TFQIGGAGRP SFTPRQAILT LQTSSSEPRS GGIGTLQFIE EFVPSVYFNP
210 220
FSGPPGHYPD QFIPNFDAVK DSADGYD
Length:227
Mass (Da):24,687
Last modified:March 1, 1992 - v1
Checksum:iC1CACF31E6F5E5D5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti160P → S no nucleotide entry (PubMed:23142869).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73260 Genomic DNA. Translation: AAA96413.1.
PIRiE39449. SXAD85.
RefSeqiAP_000217.1. AC_000008.1.

Similar proteinsi

Entry informationi

Entry nameiCAP8_ADE05
AccessioniPrimary (citable) accession number: P24936
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: August 30, 2017
This is version 59 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Caution

An interaction between hexon-linking protein IIIa and hexon-linking protein VIII as been described in PubMed:20798312. However, the subcellular location of hexon-linking protein IIIa was incorrectly assigned to the capsid interior.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families